LOCUS       sequence-490589-        5622 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  pZE12-luc containing MNX1 from Candida parapsilosis CBS604 and ubiC 
            from E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Shen X, Wang J, Wang J, Chen Z, Yuan Q, Yan Y
  TITLE     High-level De novo biosynthesis of arbutin in engineered Escherichia
            coli.
  JOURNAL   Metab Eng. 2017 Jul;42:52-58. doi: 10.1016/j.ymben.2017.06.001. Epub
            2017 Jun 2.
  PUBMED    28583673
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2017-07"; volume: "42:52-58. doi"; pages: " 
            10.1016/j.ymben.2017.06.001. Epub 2017 Jun 2"
COMMENT     Sequence Label: pSXL101
FEATURES             Location/Qualifiers
     source          1..5622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     70..89
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(334..420)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             3825..3836
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    3878..3894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3901..3917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(3940..3958)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4026..4130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4131..4199,4200..4991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4349..4368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      5021..5115
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(5203..5622,1..169)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
       61 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      121 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      181 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      241 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      301 ccgcagccga acgccctagg tctagggcgg cggatttgtc ctactcagga gagcgttcac
      361 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
      421 atgcctctag attaggtaga ggagattttg ttttcccatt tgcacgccag ttccgccagt
      481 gctttggtgg aagaaccgtc gtcagacagc gcacgggatg ccgcgtcttt caggtctttc
      541 atggtagagc ggaatttttt gccttcttcg ccttccatca ggcctttaac cgcgttcgcg
      601 atttctacgc ggccgatcag gccgttttca cccgctttcg gacgcagcgc cactttcaga
      661 ccttcagtca gcattaccgc gttcattttc tgttctgcgt acagcggcca cgcgatcagc
      721 ggcacaccgt taactacaga ttccaggata gagttccaac cgcagtgggt caggaagcca
      781 ccagtggaac cgtggctcag gatttcagtc tgcggcgccc aagaagggac cagcagacaa
      841 cgacctttgg tacgttccag gaagccttcc ggcaggtatg ccagagcgtc gttctggttt
      901 tggatgctga agtacgtggc attggcaatc ttgtcattcg ggctgcgcac aacccacagg
      961 aaacgttgtt cgctcatttc caggcccaga gccagttcaa tgaactgatt atgggacact
     1021 gcaccaccgc tgccgaaaga aatgaacaga acggaaccac gcggctggtc atccagccat
     1081 ttcagacatt cacagtcgtc aactttgctg ctggaatccg cacggatcag cgggccgatc
     1141 gggtaaaccg gcggtttacc ctggtcttcc tcttgcagag ctttcagcgg gcccggctcc
     1201 aggtcgttga aggtgttaac catgataccc tcagccaggc ggtaacgttt ggcctggtgc
     1261 agcaggcatt tgtaggcatc gtttttgcga tcctgagctg ggtccaggaa atccttaccg
     1321 tgaatcggga tgcagcctgg aatctgcagt ggttctggga cgtcacggta ttcgcaggac
     1381 accatctgat ccagcttcgg caggtgaaag aacagagaca ggcacatggc cgtcgtcgga
     1441 tagaagatat aaggggagac cttgaactca attgcaacat caaatgcatc ggtgccaaac
     1501 aggtccacca ccagagcagc cagcttggtg gtggccagca gagtcttaac tgcatcacga
     1561 acaaacggca gagagcgagt gatggtcaga caaatacggg tctcgatacg aacatcagcc
     1621 ggcaggtcat caaagctgac cggcggcagc agaacatagt ttacgcccgc cggcagagca
     1681 tccagaaaag atttctgtgc tttaggcaga ggaccatcgg ttgggataat gaaagttacg
     1741 ccgaagttgt gacgcagtac caggcgttta gcgaactcaa ccagcgggat cagatgaccc
     1801 atacccggag tcggcaccat agcgatgtgc ggggtatgct ccatggtata tctcctgcat
     1861 gcttagtaca acggtgacgc cggtaaaaac agttctgtta gcaacagcgg tttaccgctt
     1921 aatcgcaggc gggaacgtcg cccccacagc ccggcatcac ggcctatctc aataaagtcc
     1981 cgggttaatg tcgatgatgt gaacagatag cgtcctaacg gcgttttacc caatttttgt
     2041 aacgccagct ccggcccgct taacgttgac acaggaacga cggtacgacc ggcaagccac
     2101 ggttcaccat cggcacataa caaaatttca cgtaaccagt aacgagactc tttcggcagc
     2161 agcggcagtt cttcggggat ttcattctgc tcgacaaacc cttcgcggat catcgtcacg
     2221 cttaccgttt ttccctgctg ttcaaaacgt tttgtcatgg aatcctccag caacagccag
     2281 tcgagcagtt gcggatccag ggcagggatc tctttaaaat agcgcagcgc acgcagttgc
     2341 gttaacgcgg ggtgtgacat ggtatatctc ctgtcgactt agccggacgc gctcagcggc
     2401 gccgccgggg tgttacccgc cgccagggtg gcggccgcgc cgccaccgga cacgtctttt
     2461 tcaccgctgc ttttcggttt gtacaggttc gggtaggtgt aggtcgggtt accacccgcc
     2521 ttgaactcct tccacgcgcg ttccacttcg tcgaacgcgt tgtagcccca caggaagtcc
     2581 tggaacaccg ggtcagcgaa gcggttcggg cagcccagtt ccggctgttt gtcgtacagg
     2641 taagagtcgc ggatttcctg cagttcaccg tccggcaggt ggtagatgtc ctgacactgg
     2701 tgagagccgc gcaccacctg agaagagcgc catttacgca ggttctccgt catctgcagc
     2761 agctgcggga tctggtcacg cagttcgatc ttggagaaca ggccagccag cacagcaccg
     2821 tcctccaccg cctgggacgc accagaggcc aggtacggca gggtggagtg ggaagcgtca
     2881 cccaggatga taaagttgcc ggttttggag tttacccacg ttttcagttc acgggagtcc
     2941 tgcagacgcc atttagacac gctggtgatc agtttgaaaa cggtggtcag gtcctggtcc
     3001 caacctttaa ccaggtccag cagctcctct tggctggcat cctgtttcag aacgcctttc
     3061 ggcagggtgt cagggcacag ggcaacaaca ttacagattt caccttcgtg caggaaatac
     3121 attacgatgt gcatggtcgg accccaccag aagttgatgt tcgggttggc gtaaaatttc
     3181 tccaggccag gaactttctt catatcttca actttgatca gtgcacggta tgccagatca
     3241 ccagtatcgt aagcgccaga agagtcacca gtcagcagag cacgggtctg gctacgtacg
     3301 ccatcatagc ctacgattac atcaccagag tactgtttgc cagttgcagt agtcacagtt
     3361 gctttttcaa aatcgatatc aacaacacgg ctattggtgt ggatttcaac acccagttct
     3421 tcggcacgat caaccagtac tttatgataa tcagcgcggt gaatatgcag atacatgcca
     3481 tcgtattttt ccagaacata aggcaccagg ttctgcgtag acagcagttc acccttatat
     3541 ttcttgacca ggatatcatg cggatgaatg gagactttat caactgcatc cagtacgcca
     3601 attgctttca gaatcttggt gctcggcggt ggaatttgaa taccagcgcc aacttcaccc
     3661 agttcagtag ctgcttccag cagaatgaca cggtggcctg ccagacgcag gcaaatagaa
     3721 gccgcgacgc cacccagacc cgcacctaca acgacaaacg tcagttggat tggagctttt
     3781 tggaaaccat aagttttgct tggtgcctga actgccatgg tacctttctc ctctttaatg
     3841 aattcggtca gtgcgtcctg ctgatgtgct cagtatcttg ttatccgctc acaatgtcaa
     3901 ttgttatccg ctcacaattc tcgaggtgaa gacgaaaggg cctcgtgata cgcctatttt
     3961 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     4021 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4081 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4141 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4201 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4261 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4321 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4381 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4441 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4501 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4561 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4621 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4681 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4741 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4801 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4861 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4921 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4981 agcattggta actgtcagac caagtttacg agctcgcttg gactcctgtt gatagatcca
     5041 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
     5101 tttattggtg agaatccaag cactagtcat gaccaaaatc ccttaacgtg agttttcgtt
     5161 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5221 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5281 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5341 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5401 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5461 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5521 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5581 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gg
//
