LOCUS       sequence-490593-       10417 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  different combination of A. thaliana and N. tabacum Rubisco assembly
            factors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Li S, Lee B, Lao Y, Lertwiriyapiti S, Huang X, Wang T
  TITLE     Directed evolution of a plant Rubisco chaperone with altered client 
            recognition.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Sep 16;122(37):e2510701122. doi: 
            10.1073/pnas.2510701122. Epub 2025 Sep 11.
  PUBMED    40932770
REFERENCE   2  (bases 1 to 10417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-09-16"; volume: "122(37):e2510701122. 
            doi"; pages: " 10.1073/pnas.2510701122. Epub 2025 Sep 11"
COMMENT     Sequence Label: pTW411b
FEATURES             Location/Qualifiers
     source          1..10417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             86..877
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        join(1194..1199,1200..1215,1216..1222)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1230..1246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4552..4574
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             5841..5849
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             6267..6289
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    8011..8027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8035..8041,8042..8057,8058..8063))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        8306..8383
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8384..9466
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8403..8422)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      9623..10361
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        join(10411..10417,1..85)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
       61 tcaataatat tgaaaaagga agagtatgag ggaagcggtg atcgccgaag tatcgactca
      121 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      181 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      241 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      301 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      361 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      421 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      481 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      541 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      601 atggaactcg ccgcccgact gggctggaga tgagcgaaat gtagtgctta cgttgtcccg
      661 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
      721 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
      781 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
      841 cgtgaaaggc gagatcacca aggtagtcgg caaataaacg ccatgggcat taaaaaatgc
      901 cctcttgggt tatcaagagg gtcattatat ttgctgagga gtgccgttaa tgcgcaacgc
      961 aattaatgta agttagctca ctcattaggc acaattctca tgtttgacag cttatcatcg
     1021 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg
     1081 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa
     1141 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa
     1201 ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     1261 cagccagtcc gtttaggtgt tttcacgagc acttcaccaa caacgcgtac aactaggaga
     1321 tataccatgg gtatgcaaca actttatcag ccttttagac ctcctcctcc acctctccca
     1381 cccaaattcc gcaatcttga cacaaatgca aagctcgaag ttctatctaa tcgccttggt
     1441 ctttggtatg aatatgctcc tttgatacct tatttaacaa gtgaagggtt tactccttca
     1501 actcttgagg aaatcactgg ccttactgga gttgaacaga atcggttggt tgttgctgcc
     1561 caagttcgtg atactcttgt tgagtctgct gctttagatg aagaaacgtt gtcgtatttt
     1621 gaatctggag gtgctgaatt attatatgaa attcgtttgc ttagtgctag gcaacgaact
     1681 gatgcagcaa catttttagt gaagaatggg tttgatgcaa aacaagctca agatttagct
     1741 agagcaatta aagattatcc gcgtagacgt gtggattatg gttgggacaa gtttaatggt
     1801 gattctcctg gtgattgttt agcttttatg tactttagat tagctcaaga atatgctgcg
     1861 gcagcttctg aggatttgag gaggtcgtca atggagaagg cattagaagt tgttgagtct
     1921 gaaagtgcga ggaatttgct tgtgatggag ctagaaggac gtgaggttgc taaggagagt
     1981 gtacttgatg atggggtgac agtgcctttg gtgagaatga agttagggga ggtagctgag
     2041 tctacgattg tagtcgtttt gcctgtttgt aaggcggagg gaagggacgt ggaggtggag
     2101 gcggcgccgt gggaatgtgg tggcgtaggg gattttggta tagtggaggc ggagaaggat
     2161 tggaggagat gggttgtttt gcccggatgg caacctatcg cggggttaga gagaggtgga
     2221 gttgcagtgt cgttcaagag cgggaacttt ttgccgtgga gggagaagag taagtacaag
     2281 caagagccgg ttttggtagt ggcggatagg ggaaggacag aagtggcgtc ggaggacggg
     2341 ttttacttgg tggttgatgg tggagacggg agcaatgagg agggattgaa agtggaaaga
     2401 gggtcaacat tgaagaaaag aggggttgaa cagagcttag gaattgtgct tatagtagtt
     2461 aggccaccaa ggtgggaaga tgaagaacaa ttaggtgaag aagattggga ttaaattaca
     2521 aaaattagga gatatacata tgatgtctaa tctggcgcag gattttcttg gtgacttcgg
     2581 agctcgtgac ccttacccgg aggagatagc gagtcaattc ggagataaag tgttgggatg
     2641 ccaaagcact gagcacaaga ttttgatacc aaacgcatct gttttgtctc tctcccagct
     2701 tcagtgttcc cctgtttcgt cttcacagcc tcctttgtcc ggcgatgatg ccagaactct
     2761 cctccacaag gttttgggat ggagtatagt ggataatgaa gcgggtggtc tgaaaataag
     2821 gtgtatgtgg aaagtgaggg attttgggtg cggtgttgaa ctcataaaca ggatccataa
     2881 ggttgctgaa gcttctggtc attacccttc tcttcacttg gaaagtccta cccaagttcg
     2941 agctgaacta tttacctctt ctatcggagg gttgagcatg aatgatttca taatggcggc
     3001 taaaatagat gatatcaaga cttctgatct ttcccctagg aaaagagctt gggcgtgacg
     3061 gctgaaatta atgaggagat atacatatgg caaaagcatt taagggtaat tacaagaatt
     3121 taaagaagga gaaaaagagt aggaggaatc ttgtggttgt cagtgattta ggtggacagt
     3181 atgaatatgg ctttgatgat atcaaaacgc aaattcttaa ctacttcaca tttaaagcag
     3241 tgaggactat tttgaaccag cttaatgaaa tggactctcc acaatatcaa tggttcaatg
     3301 attatgttac agtaaataag cttagggatg ggaagcgttt tattcgcaac cttgctaagg
     3361 agaagcaaga actcgctgaa aaagtgatgg taacacggct cagtctgtat gcgaaatgga
     3421 taaaaaaatg tgatcacgag gagatctaca accgaatatc tgatcagaat gtggaagtga
     3481 tgcgtgagcg gctcatggaa actgtgattt ggccatcaga tgacacgaac tcggagagag
     3541 ttgggtaatt cagctaagac actgcacagg agatatacat atgatggccg caaacaataa
     3601 tcctcagggc actaaaccaa acagtttagt ttgtgcaaat tgtgaagggg aaggttgtgt
     3661 ggcatgttct caatgcaaag gaggtggtgt gaatttgatt gatcatttca atggtcagtt
     3721 taaagctggt gcactctgtt ggctttgcag aggtaaaaag gaggtgttgt gtggggactg
     3781 caatggagct ggtttcattg gtgggttctt gagcaccttc gatgagtaga gtcagtaggg
     3841 ccctaaaaaa aaaccccgcc cctgacaggg cagggttttt tttggcgaaa aaaccccgct
     3901 gaagcggggt tttttgcgta cattggctcc gtctaagaaa gtatagccat cacatctgaa
     3961 gctctgaggg caatgtagtt ggaaccatct ttgcccttga agtcgttacc agcatacttg
     4021 gagtaaagta ctgtgcttcc ggttgatact ggtagaggcg taattttacc ttcctcgtct
     4081 agggaacccg gtccaactgc tatcacggtg ccaatagaag gcttctcttt ggtagtctcg
     4141 gttaacaaca accctccagc tgttttctcc tccgcctcag caaccttaat aaagactcgg
     4201 tcattcaaag gtttgagatc tttgatgtcc tctgtctcaa gaatgccaac aatatcatct
     4261 tccttgagga taagatgctt cacatcattg aactccacct cagttcctgc gtatttggag
     4321 tagataattt gtgctccagt agggacagtg atatcaattt tgttcttccc aatagttctt
     4381 ccttcaccca cagcaacgac ttcacctcct tgaggttttg attgagcagt ggatggaagt
     4441 aagataccac ctaaagtctt ctcctctgcc tccttgatct tcaccaaaac tcgatctccc
     4501 aatggcttaa ttgaagtata cttaggggca acaacagagg ccatatgtat atctccttct
     4561 taaagttaaa caaaattatt tctagagggc aattgttatc cggtcgacgt cgaccttagt
     4621 atccgtaacc tgagttgtcc attgggttgc cgacgggaac tggttcaggt tccttgatct
     4681 caacaacaac gcagtccgac atcaagaaag tctttgcaac cgaagctgcg tgttccaagc
     4741 aacatctcac aacctttgtt ggatcgatga ttcctgcagc catcaaatcc tcgtacttgc
     4801 cggttgcagc attgtaaccg aatttcacgt tatcgttgga aagcaccttc tcgctaacta
     4861 cacttccatt aacaccagca ttcttggcga ttagttttag ggggtaactt agtgctcttt
     4921 taactatatc cgctccaacc ttttcttcat cattatcaag cgtggcttta atggcatcaa
     4981 ccttggaagc aagacgaagc agagtgcaac caccaccaac aacaatacct tcctcgacag
     5041 cggcctttgt tgcattaaga gcatcttcaa ctctcaactt cttctctttg agttctgtct
     5101 cagtttgtgc tccaacctga atcacagcaa ctccaccaga gagctttgca attctctcat
     5161 tgagtttctc cttctcgtaa tcttgctccg cttgctcaat aagattttta atctgtgtaa
     5221 cacgcttttt cactgcatcc tgtgtgcttc catcacccac tatggtcgag gtttcctttg
     5281 tgaggacaac ctttgaagca ttaccaagaa cctcttttcc agctttgtca agtgaaagac
     5341 caacttcctc acggatcaca gttgctccag ttagaatggc aatatcatca aggtattggc
     5401 tcttgcgctc tccaaaccct ggagctctga gagctgctat cttcagtgta cctctaagct
     5461 tgtttacaac aagagtcgct aaagcttctt gttcaatgtc ttcagcaata attaatattg
     5521 ggtatccgcc tctaattgca tcctccaaaa caccaacaag atcccttgca ttggtaattt
     5581 tcttgtcaac gagaagtaac ttgcaattgt cgaactcgac ggacattttc tcgctgtctg
     5641 tcacaaagta aggagagata taaccacggt caaattgcat tccttctacg acgtagaggt
     5701 tgttctcggc acttttaccc tcctcaaggg taaccacacc tttcctgccc actttgctca
     5761 ttgcttcagc aatcatattt ccaatttcgt cgttgttacc cgcactaaca gctgcaacat
     5821 ctgcaagttc actgtcttca acctccttag acattttctt gagctcggta accaaagcct
     5881 ttgctgtctt ctcaatacct ctggtgatca aaacagggtt tgcaccagca gctaccacct
     5941 tgacaccctc agcgataaaa ccttgtgcaa gaacaacaga tgttgttgta ccgtcaccag
     6001 ccaagtcatt ggttttggca gccgcttgcc tcacaagctt agcgccaatg ttctcaaccg
     6061 ggtcttccaa ctcaacctct cttgcaacag tcacaccatc attaacaatt cgtggtgatc
     6121 catacttgct ctcaagaaca acatttcgtc ctttgggtcc aagtgtaacg ccaacaagat
     6181 ctgcgagctt gttgacacca gcttgaagtc tcctaatggt agtcccatct ttgttgaaat
     6241 gtaattcctt tgctgccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag
     6301 ctccttacac catgaggccc tcaggagcag cagcagcagg agccttgggt ttcggtttgt
     6361 caacaacaat ggcctgagtg gtcagtacca ttcctgctac ggaagcagcg ttctgtagcg
     6421 cacatcttgt cactttagcc ggatcaatca ctccagcttc gaacagattc tcgtaagtat
     6481 cagtcattgc gttgtaccca ttctcccaat cgctgaacat aatcttttcc acaacaactt
     6541 ctccttcaac tccagcgttc tgagcaataa gagcagctgg tgacagcaat gccttttgta
     6601 ctatgtcagc tcccaaacgt tcatcagcat cctcaaaagt ctccttaatg gcgggaataa
     6661 cagtggagag atgcaccaaa gcagcaccac cacctggaac tatgccttcc tcgatagcag
     6721 caaatgttgc gttctttgca tcctcgatac gaagcttacg gtcctcaagc tcagtttcag
     6781 ttgctgctcc gactttaatg acagcaacac ctccagatag cttcgcaatt ctctcagcga
     6841 gcttctctga gtcatacaca gaatcagtct caaatagttc tttcttcagc tgagcaatcc
     6901 gagcttgtaa ttcgtccttg gaagctgcat ctgcaatgag ggtagtcgaa tctttgctaa
     6961 tcgtgacttt tcgggcaata cccaattgat ctatggttgc gttttcaacc agtaagctca
     7021 tgtccatggc taggtactca gctcctgtca agattgcaat atcttgaagc atggcttttc
     7081 ttctttctcc aaatcctggc gctttaacgg caacaacatt gaggacacca cgaagcttgt
     7141 tcacgacaag ggtagctaag gcttcaccag taacatcctc tgcaataatc aacaacggag
     7201 cacgaagctg agtggtcttc tccaaaatag ggatgatgtc tttgatcgca gtgatcttct
     7261 gatcagtgat caatacccta gcattctcaa actcagctag tagtttctca gggtttgtaa
     7321 caaactgagg cgagatgtaa cctctgtcaa tctccatccc ttcttcaact tcgaccgtgg
     7381 tttcaaaaga agatgaagat tcaatggaca aaacaccatc aggtccaact ttgtcgatgg
     7441 catcagcaat cattgaccct ataaggtcat catttccagc agagatagaa gccacagctc
     7501 tgatgtcatc acgacctttc acgggcctag ctttcttctg aagctcttcg atcagacctt
     7561 gaacagtctt atcaattccc ctcttgagtg aaacgggatt cgcaccagaa gtgacgctca
     7621 acaatccatg tttgattatc tcccgagcaa ggatagacgc agtggttgtc ccatcaccag
     7681 cagagtcatt agtcttactc gcaacctcac gaataagcgc tgcaccagca ttttccatgg
     7741 cattaggtaa ctcaatagct ctagcaatgg tgactccatc attcacaacc ttaggacttc
     7801 caaattcatc caacacaaca ttcctccctc tggggccaag cgtaagacca acacaatcag
     7861 caagcttatc aataccagct tgtagagcag ctctagagtg ctggtcaaaa gctatttcct
     7921 ttacattcat atgtatatct cctactgcac ctattgttgg tgaagtgctc gtgaaaacac
     7981 ctaaacggac tggctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacattat
     8041 acgagccgat gattaattgt caacagctca tttcagaata tttgccagaa ccgttatgat
     8101 gtcggcgcaa aaaacattat ccagaacggg agtgcgcctt gagcgacacg aattatgcag
     8161 tgatttacga cctgcacagc cataccacag cttccgatgg ctgcctgacg ccagaagcat
     8221 tggtgcaccg tgcagtcgat gataagctgt caaacatgag aattgtgcct aatgagtgag
     8281 ctaacttaca ttaattgcgt tgcgcgacac catcgaatgg tgcaaaacct ttcgcggtat
     8341 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
     8401 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
     8461 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     8521 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     8581 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
     8641 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
     8701 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
     8761 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
     8821 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
     8881 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
     8941 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
     9001 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
     9061 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
     9121 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
     9181 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
     9241 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
     9301 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
     9361 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9421 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgaaggg tccctaagtc
     9481 tcctcagcaa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgaccggat
     9541 gtcctcttcc tcctgggctg acttcaggtg ctacatttga agagataaat tgcactgaaa
     9601 tctagagcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9661 taatcttttg ccctgtaaac gaaaaaacca cctggggagg tggtttgatc gaaggttaag
     9721 tcagttgggg aactgcttaa ccgtggtaac tggctttcgc agagcacagc aaccaaatct
     9781 gtccttccag tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa
     9841 acaaatcctc tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt ttaccgtgct
     9901 tttccgggtt ggactcaagt gaacagttac cggataaggc gcagcagtcg ggctgaacgg
     9961 ggagttcttg cttacagccc agcttggagc gaacgaccta caccgagccg agataccagt
    10021 gtgtgagcta tgagaaagcg ccacacttcc cgtaagggag aaaggcggaa caggtatccg
    10081 gtaaacggca gggtcggaac aggagagcgc aagagggagc gacccgccgg aaacggtggg
    10141 gatctttaag tcctgtcggg tttcgcccgt actgtcagat tcatggttga gcctcacggc
    10201 tcccacagat gcaccggaaa agcgtctgtt tatgtgaact ctggcaggag ggcggagcct
    10261 atggaaaaac gccaccggcg cggccctgct gttttgcctc acatgttagt cccctgctta
    10321 tccacggaat ctgtgggtaa ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc
    10381 ggagcgtagc gaccgagtga cggccttaat ttggctt
//
