LOCUS       sequence-490596-       10396 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  different combination of A. thaliana and N. tabacum Rubisco assembly
            factors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Li S, Lee B, Lao Y, Lertwiriyapiti S, Huang X, Wang T
  TITLE     Directed evolution of a plant Rubisco chaperone with altered client 
            recognition.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Sep 16;122(37):e2510701122. doi: 
            10.1073/pnas.2510701122. Epub 2025 Sep 11.
  PUBMED    40932770
REFERENCE   2  (bases 1 to 10396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-09-16"; volume: "122(37):e2510701122. 
            doi"; pages: " 10.1073/pnas.2510701122. Epub 2025 Sep 11"
COMMENT     Sequence Label: pTW411d
FEATURES             Location/Qualifiers
     source          1..10396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             86..877
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        join(1194..1199,1200..1215,1216..1222)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1230..1246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4531..4553
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             5820..5828
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             6246..6268
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    7990..8006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8014..8020,8021..8036,8037..8042))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        8285..8362
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8363..9445
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8382..8401)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      9602..10340
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        join(10390..10396,1..85)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
       61 tcaataatat tgaaaaagga agagtatgag ggaagcggtg atcgccgaag tatcgactca
      121 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      181 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      241 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      301 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      361 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      421 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      481 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      541 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      601 atggaactcg ccgcccgact gggctggaga tgagcgaaat gtagtgctta cgttgtcccg
      661 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
      721 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
      781 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
      841 cgtgaaaggc gagatcacca aggtagtcgg caaataaacg ccatgggcat taaaaaatgc
      901 cctcttgggt tatcaagagg gtcattatat ttgctgagga gtgccgttaa tgcgcaacgc
      961 aattaatgta agttagctca ctcattaggc acaattctca tgtttgacag cttatcatcg
     1021 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg
     1081 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa
     1141 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa
     1201 ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     1261 cagccagtcc gtttaggtgt tttcacgagc acttcaccaa caacgcgtac aactaggaga
     1321 tataccatgg gtatgcaaca actttatcag ccttttagac ctcctcctcc acctctccca
     1381 cccaaattcc gcaatcttga cacaaatgca aagctcgaag ttctatctaa tcgccttggt
     1441 ctttggtatg aatatgctcc tttgatacct tatttaacaa gtgaagggtt tactccttca
     1501 actcttgagg aaatcactgg ccttactgga gttgaacaga atcggttggt tgttgctgcc
     1561 caagttcgtg atactcttgt tgagtctgct gctttagatg aagaaacgtt gtcgtatttt
     1621 gaatctggag gtgctgaatt attatatgaa attcgtttgc ttagtgctag gcaacgaact
     1681 gatgcagcaa catttttagt gaagaatggg tttgatgcaa aacaagctca agatttagct
     1741 agagcaatta aagattatcc gcgtagacgt gtggattatg gttgggacaa gtttaatggt
     1801 gattctcctg gtgattgttt agcttttatg tactttagat tagctcaaga atatgctgcg
     1861 gcagcttctg aggatttgag gaggtcgtca atggagaagg cattagaagt tgttgagtct
     1921 gaaagtgcga ggaatttgct tgtgatggag ctagaaggac gtgaggttgc taaggagagt
     1981 gtacttgatg atggggtgac agtgcctttg gtgagaatga agttagggga ggtagctgag
     2041 tctacgattg tagtcgtttt gcctgtttgt aaggcggagg gaagggacgt ggaggtggag
     2101 gcggcgccgt gggaatgtgg tggcgtaggg gattttggta tagtggaggc ggagaaggat
     2161 tggaggagat gggttgtttt gcccggatgg caacctatcg cggggttaga gagaggtgga
     2221 gttgcagtgt cgttcaagag cgggaacttt ttgccgtgga gggagaagag taagtacaag
     2281 caagagccgg ttttggtagt ggcggatagg ggaaggacag aagtggcgtc ggaggacggg
     2341 ttttacttgg tggttgatgg tggagacggg agcaatgagg agggattgaa agtggaaaga
     2401 gggtcaacat tgaagaaaag aggggttgaa cagagcttag gaattgtgct tatagtagtt
     2461 aggccaccaa ggtgggaaga tgaagaacaa ttaggtgaag aagattggga ttaaattaca
     2521 aaaattagga gatatacata tgatgctatt gggcgatttt ggggcgagag atccattccc
     2581 agctgaaata gagagtaaat tcggtgaaaa agttttgggt aatactgaca cggagcacaa
     2641 aattctcatc ccagctgctt ctgctctgtc ccttgctcag caagactgca ttgtcatctc
     2701 acctggtcaa actcctctct cggaatatga agctcgacag ctcctattca aggttgttgg
     2761 ctggagactg gcacatgaag atggggtgct caagctacaa tgtacatgga aattaagaga
     2821 cttcgaatgt ggggttgaac ttatcaatcg aataggcaaa gtggtggaag gcactgggca
     2881 cgttcccaca cttcttcact tagagcagtc caatcaagtt cgtgctgagc tatggactgc
     2941 ttccatcgga ggtttgagca taaacgattt cattgtagct gctaaaatag atcagataaa
     3001 gacatcggac cttgtcccaa gaaaaagagt ttgggcataa cggctgaaat taatgaggag
     3061 atatacatat ggcaaaagca tttaagggta attacaagaa tttaaagaag gagaaaaaga
     3121 gtaggaggaa tcttgtggtt gtcagtgatt taggtggaca gtatgaatat ggctttgatg
     3181 atatcaaaac gcaaattctt aactacttca catttaaagc agtgaggact attttgaacc
     3241 agcttaatga aatggactct ccacaatatc aatggttcaa tgattatgtt acagtaaata
     3301 agcttaggga tgggaagcgt tttattcgca accttgctaa ggagaagcaa gaactcgctg
     3361 aaaaagtgat ggtaacacgg ctcagtctgt atgcgaaatg gataaaaaaa tgtgatcacg
     3421 aggagatcta caaccgaata tctgatcaga atgtggaagt gatgcgtgag cggctcatgg
     3481 aaactgtgat ttggccatca gatgacacga actcggagag agttgggtaa ttcagctaag
     3541 acactgcaca ggagatatac atatgatggc cactgacagt gataaagaca cgaaagtaag
     3601 gagcatagtc tgtcaaaatt gtgatggaaa tggtgcagtg tcgtgctcac agtgcaaagg
     3661 ctctggggtt aactctgtag atcatttcaa cgggcggttc aaagctggtg gactatgttg
     3721 gttgtgcaga ggtaaaaaag atgtgttatg tggtgactgc aatggtgctg gatttcttgg
     3781 tggatttatg agtacgttcg atgagtagag tcagtagggc cctaaaaaaa aaccccgccc
     3841 ctgacagggc agggtttttt ttggcgaaaa aaccccgctg aagcggggtt ttttgcgtac
     3901 attggctccg tctaagaaag tatagccatc acatctgaag ctctgagggc aatgtagttg
     3961 gaaccatctt tgcccttgaa gtcgttacca gcatacttgg agtaaagtac tgtgcttccg
     4021 gttgatactg gtagaggcgt aattttacct tcctcgtcta gggaacccgg tccaactgct
     4081 atcacggtgc caatagaagg cttctctttg gtagtctcgg ttaacaacaa ccctccagct
     4141 gttttctcct ccgcctcagc aaccttaata aagactcggt cattcaaagg tttgagatct
     4201 ttgatgtcct ctgtctcaag aatgccaaca atatcatctt ccttgaggat aagatgcttc
     4261 acatcattga actccacctc agttcctgcg tatttggagt agataatttg tgctccagta
     4321 gggacagtga tatcaatttt gttcttccca atagttcttc cttcacccac agcaacgact
     4381 tcacctcctt gaggttttga ttgagcagtg gatggaagta agataccacc taaagtcttc
     4441 tcctctgcct ccttgatctt caccaaaact cgatctccca atggcttaat tgaagtatac
     4501 ttaggggcaa caacagaggc catatgtata tctccttctt aaagttaaac aaaattattt
     4561 ctagagggca attgttatcc ggtcgacgtc gaccttagta tccgtaacct gagttgtcca
     4621 ttgggttgcc gacgggaact ggttcaggtt ccttgatctc aacaacaacg cagtccgaca
     4681 tcaagaaagt ctttgcaacc gaagctgcgt gttccaagca acatctcaca acctttgttg
     4741 gatcgatgat tcctgcagcc atcaaatcct cgtacttgcc ggttgcagca ttgtaaccga
     4801 atttcacgtt atcgttggaa agcaccttct cgctaactac acttccatta acaccagcat
     4861 tcttggcgat tagttttagg gggtaactta gtgctctttt aactatatcc gctccaacct
     4921 tttcttcatc attatcaagc gtggctttaa tggcatcaac cttggaagca agacgaagca
     4981 gagtgcaacc accaccaaca acaatacctt cctcgacagc ggcctttgtt gcattaagag
     5041 catcttcaac tctcaacttc ttctctttga gttctgtctc agtttgtgct ccaacctgaa
     5101 tcacagcaac tccaccagag agctttgcaa ttctctcatt gagtttctcc ttctcgtaat
     5161 cttgctccgc ttgctcaata agatttttaa tctgtgtaac acgctttttc actgcatcct
     5221 gtgtgcttcc atcacccact atggtcgagg tttcctttgt gaggacaacc tttgaagcat
     5281 taccaagaac ctcttttcca gctttgtcaa gtgaaagacc aacttcctca cggatcacag
     5341 ttgctccagt tagaatggca atatcatcaa ggtattggct cttgcgctct ccaaaccctg
     5401 gagctctgag agctgctatc ttcagtgtac ctctaagctt gtttacaaca agagtcgcta
     5461 aagcttcttg ttcaatgtct tcagcaataa ttaatattgg gtatccgcct ctaattgcat
     5521 cctccaaaac accaacaaga tcccttgcat tggtaatttt cttgtcaacg agaagtaact
     5581 tgcaattgtc gaactcgacg gacattttct cgctgtctgt cacaaagtaa ggagagatat
     5641 aaccacggtc aaattgcatt ccttctacga cgtagaggtt gttctcggca cttttaccct
     5701 cctcaagggt aaccacacct ttcctgccca ctttgctcat tgcttcagca atcatatttc
     5761 caatttcgtc gttgttaccc gcactaacag ctgcaacatc tgcaagttca ctgtcttcaa
     5821 cctccttaga cattttcttg agctcggtaa ccaaagcctt tgctgtcttc tcaatacctc
     5881 tggtgatcaa aacagggttt gcaccagcag ctaccacctt gacaccctca gcgataaaac
     5941 cttgtgcaag aacaacagat gttgttgtac cgtcaccagc caagtcattg gttttggcag
     6001 ccgcttgcct cacaagctta gcgccaatgt tctcaaccgg gtcttccaac tcaacctctc
     6061 ttgcaacagt cacaccatca ttaacaattc gtggtgatcc atacttgctc tcaagaacaa
     6121 catttcgtcc tttgggtcca agtgtaacgc caacaagatc tgcgagcttg ttgacaccag
     6181 cttgaagtct cctaatggta gtcccatctt tgttgaaatg taattccttt gctgccatat
     6241 gtatatctcc ttcttaaagt taaacaaaat tatttctagc tccttacacc atgaggccct
     6301 caggagcagc agcagcagga gccttgggtt tcggtttgtc aacaacaatg gcctgagtgg
     6361 tcagtaccat tcctgctacg gaagcagcgt tctgtagcgc acatcttgtc actttagccg
     6421 gatcaatcac tccagcttcg aacagattct cgtaagtatc agtcattgcg ttgtacccat
     6481 tctcccaatc gctgaacata atcttttcca caacaacttc tccttcaact ccagcgttct
     6541 gagcaataag agcagctggt gacagcaatg ccttttgtac tatgtcagct cccaaacgtt
     6601 catcagcatc ctcaaaagtc tccttaatgg cgggaataac agtggagaga tgcaccaaag
     6661 cagcaccacc acctggaact atgccttcct cgatagcagc aaatgttgcg ttctttgcat
     6721 cctcgatacg aagcttacgg tcctcaagct cagtttcagt tgctgctccg actttaatga
     6781 cagcaacacc tccagatagc ttcgcaattc tctcagcgag cttctctgag tcatacacag
     6841 aatcagtctc aaatagttct ttcttcagct gagcaatccg agcttgtaat tcgtccttgg
     6901 aagctgcatc tgcaatgagg gtagtcgaat ctttgctaat cgtgactttt cgggcaatac
     6961 ccaattgatc tatggttgcg ttttcaacca gtaagctcat gtccatggct aggtactcag
     7021 ctcctgtcaa gattgcaata tcttgaagca tggcttttct tctttctcca aatcctggcg
     7081 ctttaacggc aacaacattg aggacaccac gaagcttgtt cacgacaagg gtagctaagg
     7141 cttcaccagt aacatcctct gcaataatca acaacggagc acgaagctga gtggtcttct
     7201 ccaaaatagg gatgatgtct ttgatcgcag tgatcttctg atcagtgatc aataccctag
     7261 cattctcaaa ctcagctagt agtttctcag ggtttgtaac aaactgaggc gagatgtaac
     7321 ctctgtcaat ctccatccct tcttcaactt cgaccgtggt ttcaaaagaa gatgaagatt
     7381 caatggacaa aacaccatca ggtccaactt tgtcgatggc atcagcaatc attgacccta
     7441 taaggtcatc atttccagca gagatagaag ccacagctct gatgtcatca cgacctttca
     7501 cgggcctagc tttcttctga agctcttcga tcagaccttg aacagtctta tcaattcccc
     7561 tcttgagtga aacgggattc gcaccagaag tgacgctcaa caatccatgt ttgattatct
     7621 cccgagcaag gatagacgca gtggttgtcc catcaccagc agagtcatta gtcttactcg
     7681 caacctcacg aataagcgct gcaccagcat tttccatggc attaggtaac tcaatagctc
     7741 tagcaatggt gactccatca ttcacaacct taggacttcc aaattcatcc aacacaacat
     7801 tcctccctct ggggccaagc gtaagaccaa cacaatcagc aagcttatca ataccagctt
     7861 gtagagcagc tctagagtgc tggtcaaaag ctatttcctt tacattcata tgtatatctc
     7921 ctactgcacc tattgttggt gaagtgctcg tgaaaacacc taaacggact ggctgtttcc
     7981 tgtgtgaaat tgttatccgc tcacaattcc acacattata cgagccgatg attaattgtc
     8041 aacagctcat ttcagaatat ttgccagaac cgttatgatg tcggcgcaaa aaacattatc
     8101 cagaacggga gtgcgccttg agcgacacga attatgcagt gatttacgac ctgcacagcc
     8161 ataccacagc ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt gcagtcgatg
     8221 ataagctgtc aaacatgaga attgtgccta atgagtgagc taacttacat taattgcgtt
     8281 gcgcgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
     8341 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
     8401 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
     8461 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
     8521 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
     8581 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
     8641 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
     8701 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
     8761 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
     8821 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
     8881 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
     8941 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
     9001 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
     9061 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
     9121 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
     9181 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
     9241 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     9301 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     9361 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     9421 ttcccgactg gaaagcgggc agtgaagggt ccctaagtct cctcagcaaa acgaaaggcc
     9481 cagtctttcg actgagcctt tcgttttatt tgaccggatg tcctcttcct cctgggctga
     9541 cttcaggtgc tacatttgaa gagataaatt gcactgaaat ctagagcggt tcagtagaaa
     9601 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatcttttgc cctgtaaacg
     9661 aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt cagttgggga actgcttaac
     9721 cgtggtaact ggctttcgca gagcacagca accaaatctg tccttccagt gtagccggac
     9781 tttggcgcac acttcaagag caaccgcgtg tttagctaaa caaatcctct gcgaactccc
     9841 agttaccaat ggctgctgcc agtggcgttt taccgtgctt ttccgggttg gactcaagtg
     9901 aacagttacc ggataaggcg cagcagtcgg gctgaacggg gagttcttgc ttacagccca
     9961 gcttggagcg aacgacctac accgagccga gataccagtg tgtgagctat gagaaagcgc
    10021 cacacttccc gtaagggaga aaggcggaac aggtatccgg taaacggcag ggtcggaaca
    10081 ggagagcgca agagggagcg acccgccgga aacggtgggg atctttaagt cctgtcgggt
    10141 ttcgcccgta ctgtcagatt catggttgag cctcacggct cccacagatg caccggaaaa
    10201 gcgtctgttt atgtgaactc tggcaggagg gcggagccta tggaaaaacg ccaccggcgc
    10261 ggccctgctg ttttgcctca catgttagtc ccctgcttat ccacggaatc tgtgggtaac
    10321 tttgtatgtg tccgcagcgc ccgccgcagt ctcacgcccg gagcgtagcg accgagtgac
    10381 ggccttaatt tggctt
//
