LOCUS       sequence-490599-       10093 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  Golden gate acceptor for AtRaf1 library cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10093)
  AUTHORS   Li S, Lee B, Lao Y, Lertwiriyapiti S, Huang X, Wang T
  TITLE     Directed evolution of a plant Rubisco chaperone with altered client 
            recognition.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Sep 16;122(37):e2510701122. doi: 
            10.1073/pnas.2510701122. Epub 2025 Sep 11.
  PUBMED    40932770
REFERENCE   2  (bases 1 to 10093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-09-16"; volume: "122(37):e2510701122. 
            doi"; pages: " 10.1073/pnas.2510701122. Epub 2025 Sep 11"
COMMENT     Sequence Label: pTW368z
FEATURES             Location/Qualifiers
     source          1..10093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             86..877
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        join(1194..1199,1200..1215,1216..1222)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1230..1246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2124..2178
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    2125..2143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2155..2173
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2179..2197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             4228..4250
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             5517..5525
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             5943..5965
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    7687..7703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7711..7717,7718..7733,7734..7739))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        7982..8059
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8060..9142
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8079..8098)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      9299..10037
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        join(10087..10093,1..85)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
       61 tcaataatat tgaaaaagga agagtatgag ggaagcggtg atcgccgaag tatcgactca
      121 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      181 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      241 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      301 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      361 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      421 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      481 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      541 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      601 atggaactcg ccgcccgact gggctggaga tgagcgaaat gtagtgctta cgttgtcccg
      661 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
      721 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
      781 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
      841 cgtgaaaggc gagatcacca aggtagtcgg caaataaacg ccatgggcat taaaaaatgc
      901 cctcttgggt tatcaagagg gtcattatat ttgctgagga gtgccgttaa tgcgcaacgc
      961 aattaatgta agttagctca ctcattaggc acaattctca tgtttgacag cttatcatcg
     1021 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg
     1081 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa
     1141 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa
     1201 ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     1261 cagccagtcc gtttaggtgt tttcacgagc acttcaccaa caacgcgtac aactaggaga
     1321 tataccatgg gtagagacct tatgagcctt tatacagttc gtccataccg tgcgtgatgc
     1381 cggccgcggt cacgaattcc agcaggacca tgtggtcacg cttttcgttc ggatctttac
     1441 tcagcacaga ctgcgtgctc aggtaatgat tgtccggcag caggaccggg ccatcaccaa
     1501 tcggggtgtt ttgctgatag tggtcggcca gttgaacgct gccatcttcc acattatggc
     1561 ggatcttgaa gttagccttg ataccgttct tctgcttatc tgcggtgatg tacacgttgt
     1621 gcgaattgaa gttatattcc agtttatggc ccagaatatt accgtcttcc ttaaaatcga
     1681 tgcctttcag ttcaatacgg ttaaccagcg tatcaccttc gaatttcact tcggcgcgcg
     1741 ttttgtaggt gccgtcatct ttaaatgaga tggtacgttc ttggacataa ccttccggca
     1801 tctcggactt gaaaaagtcg tggcgtttca tgtgatccgg gtaacgactg aaacactgaa
     1861 caccatacgt cagcgtggtg accagggtcg gccacggaac cggcagttta cccgtggtgc
     1921 aaataaactt cagcgtcagt ttaccattgg ttgcatcgcc ttcaccttcg ccgcgcacgc
     1981 tgaatttgtg accattgaca tcaccatcca gttccaccag aatcggcacc acgcccgtaa
     2041 acagttcttc gcccttgctc attttttttt cctccttact gcacctgcag gtctagattt
     2101 ttgtcgaact attcatttca cttttctcta tcactgatag ggagtggtaa aataactcta
     2161 tcaatgatag agtgtcaatc tctatcactg atagggatca tctggccatt cgatggggtc
     2221 tctattacaa aaattaggag atatacatat gatgtctaat ctggcgcagg attttcttgg
     2281 tgacttcgga gctcgtgacc cttacccgga ggagatagcg agtcaattcg gagataaagt
     2341 gttgggatgc caaagcactg agcacaagat tttgatacca aacgcatctg ttttgtctct
     2401 ctcccagctt cagtgttccc ctgtttcgtc ttcacagcct cctttgtccg gcgatgatgc
     2461 cagaactctc ctccacaagg ttttgggatg gagtatagtg gataatgaag cgggtggtct
     2521 gaaaataagg tgtatgtgga aagtgaggga ttttgggtgc ggtgttgaac tcataaacag
     2581 gatccataag gttgctgaag cttctggtca ttacccttct cttcacttgg aaagtcctac
     2641 ccaagttcga gctgaactat ttacctcttc tatcggaggg ttgagcatga atgatttcat
     2701 aatggcggct aaaatagatg atatcaagac ttctgatctt tcccctagga aaagagcttg
     2761 ggcgtgacgg ctgaaattaa tgaggagata tacatatggt atcgagaatt gcgaatcgaa
     2821 agagcaaaaa gctcttgatt gtgaatgagg atgttgctgg taattacgac gataccttcg
     2881 gtgatgtaca aaagcaaatt gttaattatt ttacatacaa agctgtgagg acggttcttc
     2941 atcagctgta cgaaatgaat cctcctcaat acacttggtt ctataatcat atcataacaa
     3001 acagaccgac ggatggcaaa cgtttcctcc gtgccctcgg caaggagagt caggagcttg
     3061 cagaaagagt gatgatcacg cgtcttcact tgtatggcaa atggatcaag aaatgcgacc
     3121 atgggaagat ataccaggaa atatcggatg aaaacttggc gttgatgcgt gaacgcctga
     3181 tggagacagt gatatggcct tccgatgaca caaactcaag gtaattcagc taagacactg
     3241 cacaggagat atacatatga tggccgcaaa caataatcct cagggcacta aaccaaacag
     3301 tttagtttgt gcaaattgtg aaggggaagg ttgtgtggca tgttctcaat gcaaaggagg
     3361 tggtgtgaat ttgattgatc atttcaatgg tcagtttaaa gctggtgcac tctgttggct
     3421 ttgcagaggt aaaaaggagg tgttgtgtgg ggactgcaat ggagctggtt tcattggtgg
     3481 gttcttgagc accttcgatg agtagagtca gtagggccct aaaaaaaaac cccgcccctg
     3541 acagggcagg gttttttttg gcgaaaaaac cccgctgaag cggggttttt tgcgtacatt
     3601 ggctccgtct aagaaagtat agccatcaca tctgaagctc tgagggcaat gtagttggaa
     3661 ccatctttgc ccttgaagtc gttaccagca tacttggagt aaagtactgt gcttccggtt
     3721 gatactggta gaggcgtaat tttaccttcc tcgtctaggg aacccggtcc aactgctatc
     3781 acggtgccaa tagaaggctt ctctttggta gtctcggtta acaacaaccc tccagctgtt
     3841 ttctcctccg cctcagcaac cttaataaag actcggtcat tcaaaggttt gagatctttg
     3901 atgtcctctg tctcaagaat gccaacaata tcatcttcct tgaggataag atgcttcaca
     3961 tcattgaact ccacctcagt tcctgcgtat ttggagtaga taatttgtgc tccagtaggg
     4021 acagtgatat caattttgtt cttcccaata gttcttcctt cacccacagc aacgacttca
     4081 cctccttgag gttttgattg agcagtggat ggaagtaaga taccacctaa agtcttctcc
     4141 tctgcctcct tgatcttcac caaaactcga tctcccaatg gcttaattga agtatactta
     4201 ggggcaacaa cagaggccat atgtatatct ccttcttaaa gttaaacaaa attatttcta
     4261 gagggcaatt gttatccggt cgacgtcgac cttagtatcc gtaacctgag ttgtccattg
     4321 ggttgccgac gggaactggt tcaggttcct tgatctcaac aacaacgcag tccgacatca
     4381 agaaagtctt tgcaaccgaa gctgcgtgtt ccaagcaaca tctcacaacc tttgttggat
     4441 cgatgattcc tgcagccatc aaatcctcgt acttgccggt tgcagcattg taaccgaatt
     4501 tcacgttatc gttggaaagc accttctcgc taactacact tccattaaca ccagcattct
     4561 tggcgattag ttttaggggg taacttagtg ctcttttaac tatatccgct ccaacctttt
     4621 cttcatcatt atcaagcgtg gctttaatgg catcaacctt ggaagcaaga cgaagcagag
     4681 tgcaaccacc accaacaaca ataccttcct cgacagcggc ctttgttgca ttaagagcat
     4741 cttcaactct caacttcttc tctttgagtt ctgtctcagt ttgtgctcca acctgaatca
     4801 cagcaactcc accagagagc tttgcaattc tctcattgag tttctccttc tcgtaatctt
     4861 gctccgcttg ctcaataaga tttttaatct gtgtaacacg ctttttcact gcatcctgtg
     4921 tgcttccatc acccactatg gtcgaggttt cctttgtgag gacaaccttt gaagcattac
     4981 caagaacctc ttttccagct ttgtcaagtg aaagaccaac ttcctcacgg atcacagttg
     5041 ctccagttag aatggcaata tcatcaaggt attggctctt gcgctctcca aaccctggag
     5101 ctctgagagc tgctatcttc agtgtacctc taagcttgtt tacaacaaga gtcgctaaag
     5161 cttcttgttc aatgtcttca gcaataatta atattgggta tccgcctcta attgcatcct
     5221 ccaaaacacc aacaagatcc cttgcattgg taattttctt gtcaacgaga agtaacttgc
     5281 aattgtcgaa ctcgacggac attttctcgc tgtctgtcac aaagtaagga gagatataac
     5341 cacggtcaaa ttgcattcct tctacgacgt agaggttgtt ctcggcactt ttaccctcct
     5401 caagggtaac cacacctttc ctgcccactt tgctcattgc ttcagcaatc atatttccaa
     5461 tttcgtcgtt gttacccgca ctaacagctg caacatctgc aagttcactg tcttcaacct
     5521 ccttagacat tttcttgagc tcggtaacca aagcctttgc tgtcttctca atacctctgg
     5581 tgatcaaaac agggtttgca ccagcagcta ccaccttgac accctcagcg ataaaacctt
     5641 gtgcaagaac aacagatgtt gttgtaccgt caccagccaa gtcattggtt ttggcagccg
     5701 cttgcctcac aagcttagcg ccaatgttct caaccgggtc ttccaactca acctctcttg
     5761 caacagtcac accatcatta acaattcgtg gtgatccata cttgctctca agaacaacat
     5821 ttcgtccttt gggtccaagt gtaacgccaa caagatctgc gagcttgttg acaccagctt
     5881 gaagtctcct aatggtagtc ccatctttgt tgaaatgtaa ttcctttgct gccatatgta
     5941 tatctccttc ttaaagttaa acaaaattat ttctagctcc ttacaccatg aggccctcag
     6001 gagcagcagc agcaggagcc ttgggtttcg gtttgtcaac aacaatggcc tgagtggtca
     6061 gtaccattcc tgctacggaa gcagcgttct gtagcgcaca tcttgtcact ttagccggat
     6121 caatcactcc agcttcgaac agattctcgt aagtatcagt cattgcgttg tacccattct
     6181 cccaatcgct gaacataatc ttttccacaa caacttctcc ttcaactcca gcgttctgag
     6241 caataagagc agctggtgac agcaatgcct tttgtactat gtcagctccc aaacgttcat
     6301 cagcatcctc aaaagtctcc ttaatggcgg gaataacagt ggagagatgc accaaagcag
     6361 caccaccacc tggaactatg ccttcctcga tagcagcaaa tgttgcgttc tttgcatcct
     6421 cgatacgaag cttacggtcc tcaagctcag tttcagttgc tgctccgact ttaatgacag
     6481 caacacctcc agatagcttc gcaattctct cagcgagctt ctctgagtca tacacagaat
     6541 cagtctcaaa tagttctttc ttcagctgag caatccgagc ttgtaattcg tccttggaag
     6601 ctgcatctgc aatgagggta gtcgaatctt tgctaatcgt gacttttcgg gcaataccca
     6661 attgatctat ggttgcgttt tcaaccagta agctcatgtc catggctagg tactcagctc
     6721 ctgtcaagat tgcaatatct tgaagcatgg cttttcttct ttctccaaat cctggcgctt
     6781 taacggcaac aacattgagg acaccacgaa gcttgttcac gacaagggta gctaaggctt
     6841 caccagtaac atcctctgca ataatcaaca acggagcacg aagctgagtg gtcttctcca
     6901 aaatagggat gatgtctttg atcgcagtga tcttctgatc agtgatcaat accctagcat
     6961 tctcaaactc agctagtagt ttctcagggt ttgtaacaaa ctgaggcgag atgtaacctc
     7021 tgtcaatctc catcccttct tcaacttcga ccgtggtttc aaaagaagat gaagattcaa
     7081 tggacaaaac accatcaggt ccaactttgt cgatggcatc agcaatcatt gaccctataa
     7141 ggtcatcatt tccagcagag atagaagcca cagctctgat gtcatcacga cctttcacgg
     7201 gcctagcttt cttctgaagc tcttcgatca gaccttgaac agtcttatca attcccctct
     7261 tgagtgaaac gggattcgca ccagaagtga cgctcaacaa tccatgtttg attatctccc
     7321 gagcaaggat agacgcagtg gttgtcccat caccagcaga gtcattagtc ttactcgcaa
     7381 cctcacgaat aagcgctgca ccagcatttt ccatggcatt aggtaactca atagctctag
     7441 caatggtgac tccatcattc acaaccttag gacttccaaa ttcatccaac acaacattcc
     7501 tccctctggg gccaagcgta agaccaacac aatcagcaag cttatcaata ccagcttgta
     7561 gagcagctct agagtgctgg tcaaaagcta tttcctttac attcatatgt atatctccta
     7621 ctgcacctat tgttggtgaa gtgctcgtga aaacacctaa acggactggc tgtttcctgt
     7681 gtgaaattgt tatccgctca caattccaca cattatacga gccgatgatt aattgtcaac
     7741 agctcatttc agaatatttg ccagaaccgt tatgatgtcg gcgcaaaaaa cattatccag
     7801 aacgggagtg cgccttgagc gacacgaatt atgcagtgat ttacgacctg cacagccata
     7861 ccacagcttc cgatggctgc ctgacgccag aagcattggt gcaccgtgca gtcgatgata
     7921 agctgtcaaa catgagaatt gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg
     7981 cgacaccatc gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag
     8041 tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg
     8101 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
     8161 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
     8221 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
     8281 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
     8341 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct
     8401 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
     8461 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
     8521 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
     8581 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
     8641 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
     8701 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
     8761 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
     8821 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
     8881 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
     8941 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
     9001 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
     9061 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9121 ccgactggaa agcgggcagt gaagggtccc taagtctcct cagcaaaacg aaaggcccag
     9181 tctttcgact gagcctttcg ttttatttga ccggatgtcc tcttcctcct gggctgactt
     9241 caggtgctac atttgaagag ataaattgca ctgaaatcta gagcggttca gtagaaaaga
     9301 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat cttttgccct gtaaacgaaa
     9361 aaaccacctg gggaggtggt ttgatcgaag gttaagtcag ttggggaact gcttaaccgt
     9421 ggtaactggc tttcgcagag cacagcaacc aaatctgtcc ttccagtgta gccggacttt
     9481 ggcgcacact tcaagagcaa ccgcgtgttt agctaaacaa atcctctgcg aactcccagt
     9541 taccaatggc tgctgccagt ggcgttttac cgtgcttttc cgggttggac tcaagtgaac
     9601 agttaccgga taaggcgcag cagtcgggct gaacggggag ttcttgctta cagcccagct
     9661 tggagcgaac gacctacacc gagccgagat accagtgtgt gagctatgag aaagcgccac
     9721 acttcccgta agggagaaag gcggaacagg tatccggtaa acggcagggt cggaacagga
     9781 gagcgcaaga gggagcgacc cgccggaaac ggtggggatc tttaagtcct gtcgggtttc
     9841 gcccgtactg tcagattcat ggttgagcct cacggctccc acagatgcac cggaaaagcg
     9901 tctgtttatg tgaactctgg caggagggcg gagcctatgg aaaaacgcca ccggcgcggc
     9961 cctgctgttt tgcctcacat gttagtcccc tgcttatcca cggaatctgt gggtaacttt
    10021 gtatgtgtcc gcagcgcccg ccgcagtctc acgcccggag cgtagcgacc gagtgacggc
    10081 cttaatttgg ctt
//
