LOCUS       sequence-490601-       10405 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  different combination of A. thaliana and N. tabacum Rubisco assembly
            factors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Li S, Lee B, Lao Y, Lertwiriyapiti S, Huang X, Wang T
  TITLE     Directed evolution of a plant Rubisco chaperone with altered client 
            recognition.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Sep 16;122(37):e2510701122. doi: 
            10.1073/pnas.2510701122. Epub 2025 Sep 11.
  PUBMED    40932770
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-09-16"; volume: "122(37):e2510701122. 
            doi"; pages: " 10.1073/pnas.2510701122. Epub 2025 Sep 11"
COMMENT     Sequence Label: pTW411a
FEATURES             Location/Qualifiers
     source          1..10405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             86..877
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        join(1194..1199,1200..1215,1216..1222)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1230..1246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4540..4562
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             5829..5837
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             6255..6277
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    7999..8015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8023..8029,8030..8045,8046..8051))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        8294..8371
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8372..9454
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8391..8410)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      9611..10349
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        join(10399..10405,1..85)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
       61 tcaataatat tgaaaaagga agagtatgag ggaagcggtg atcgccgaag tatcgactca
      121 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      181 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      241 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      301 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      361 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      421 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      481 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      541 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      601 atggaactcg ccgcccgact gggctggaga tgagcgaaat gtagtgctta cgttgtcccg
      661 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
      721 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
      781 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
      841 cgtgaaaggc gagatcacca aggtagtcgg caaataaacg ccatgggcat taaaaaatgc
      901 cctcttgggt tatcaagagg gtcattatat ttgctgagga gtgccgttaa tgcgcaacgc
      961 aattaatgta agttagctca ctcattaggc acaattctca tgtttgacag cttatcatcg
     1021 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg
     1081 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa
     1141 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa
     1201 ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     1261 cagccagtcc gtttaggtgt tttcacgagc acttcaccaa caacgcgtac aactaggaga
     1321 tataccatgg gtatgcaaca gctctaccaa ccgttccgac caccgtcatc tcccattcca
     1381 acccaattcc gttcactcga ctccgccggt aaaatcgaaa tcctcgccgg tcgaatggct
     1441 ctctggttcg aatacgcacc tcttatctct tctctttaca ccgatggttt cactcctcca
     1501 accatcgaag aactcaccgg aatctcaagc atcgaacaga accgtttaat cgtcggtgcg
     1561 caagttcgtg actcaattct tcaatctatc catgaaccgg aacttatttc tgcgttcgac
     1621 accggtggtg cagagcttct atacgaaatt cgtctcctta gcaccacgca gcgtgttgca
     1681 gccgccacat tcatcattga tcgtaatatt gattcgaaag gagcacagga tttggcacga
     1741 gccatcaagg attatcctaa ccgtcgtgga gacgttggat ggttggattt tgattacaac
     1801 ttaccaggag attgtctttc attcttgtat tacaggcaga gcagagagaa taagaatcca
     1861 tcggatcaga gaacctcgat gcttctacaa gcattggggg ttgcagaatc agaaaaggct
     1921 aagaacaggt tgaacacaga gctatacggg gacaaggaag cagagaagga gaaggagaag
     1981 aagaagaagg aagaggaagt caaagctatt cggattccag tggtgaggct taagtttgga
     2041 gaagtagcag aagcgacctc ggtggtagtt ttaccggttt gtaaagcaga ggaaggggaa
     2101 aagaagattc ttgaagctcc aatggagatc atagccggag gggattttaa ggtggttgag
     2161 gcggagaaag gatggaagag atgggtagtg cttccgtcgt ggaacccagt ggcagccatt
     2221 gggaaaggcg gtgtggcggt ttctttcagg gatgatagaa aagtgctgcc ttgggatgga
     2281 aaggaggagc ctttactggt agtggccgat agggtgagga atgttgtgga ggctgatgac
     2341 gggtattatc tcgtggtggc tgagaacgga cttaagctag agaaaggatc agatttgaag
     2401 gcgagagagg tgaaggagag tttagggatg gttgttttgg tggtgaggcc gccaagagaa
     2461 gatgatgatg attggcagac aagtcatcag aactgggact gaattacaaa aattaggaga
     2521 tatacatatg atgtctaatc tggcgcagga ttttcttggt gacttcggag ctcgtgaccc
     2581 ttacccggag gagatagcga gtcaattcgg agataaagtg ttgggatgcc aaagcactga
     2641 gcacaagatt ttgataccaa acgcatctgt tttgtctctc tcccagcttc agtgttcccc
     2701 tgtttcgtct tcacagcctc ctttgtccgg cgatgatgcc agaactctcc tccacaaggt
     2761 tttgggatgg agtatagtgg ataatgaagc gggtggtctg aaaataaggt gtatgtggaa
     2821 agtgagggat tttgggtgcg gtgttgaact cataaacagg atccataagg ttgctgaagc
     2881 ttctggtcat tacccttctc ttcacttgga aagtcctacc caagttcgag ctgaactatt
     2941 tacctcttct atcggagggt tgagcatgaa tgatttcata atggcggcta aaatagatga
     3001 tatcaagact tctgatcttt cccctaggaa aagagcttgg gcgtgacggc tgaaattaat
     3061 gaggagatat acatatggca aaagcattta agggtaatta caagaattta aagaaggaga
     3121 aaaagagtag gaggaatctt gtggttgtca gtgatttagg tggacagtat gaatatggct
     3181 ttgatgatat caaaacgcaa attcttaact acttcacatt taaagcagtg aggactattt
     3241 tgaaccagct taatgaaatg gactctccac aatatcaatg gttcaatgat tatgttacag
     3301 taaataagct tagggatggg aagcgtttta ttcgcaacct tgctaaggag aagcaagaac
     3361 tcgctgaaaa agtgatggta acacggctca gtctgtatgc gaaatggata aaaaaatgtg
     3421 atcacgagga gatctacaac cgaatatctg atcagaatgt ggaagtgatg cgtgagcggc
     3481 tcatggaaac tgtgatttgg ccatcagatg acacgaactc ggagagagtt gggtaattca
     3541 gctaagacac tgcacaggag atatacatat gatggccgca aacaataatc ctcagggcac
     3601 taaaccaaac agtttagttt gtgcaaattg tgaaggggaa ggttgtgtgg catgttctca
     3661 atgcaaagga ggtggtgtga atttgattga tcatttcaat ggtcagttta aagctggtgc
     3721 actctgttgg ctttgcagag gtaaaaagga ggtgttgtgt ggggactgca atggagctgg
     3781 tttcattggt gggttcttga gcaccttcga tgagtagagt cagtagggcc ctaaaaaaaa
     3841 accccgcccc tgacagggca gggttttttt tggcgaaaaa accccgctga agcggggttt
     3901 tttgcgtaca ttggctccgt ctaagaaagt atagccatca catctgaagc tctgagggca
     3961 atgtagttgg aaccatcttt gcccttgaag tcgttaccag catacttgga gtaaagtact
     4021 gtgcttccgg ttgatactgg tagaggcgta attttacctt cctcgtctag ggaacccggt
     4081 ccaactgcta tcacggtgcc aatagaaggc ttctctttgg tagtctcggt taacaacaac
     4141 cctccagctg ttttctcctc cgcctcagca accttaataa agactcggtc attcaaaggt
     4201 ttgagatctt tgatgtcctc tgtctcaaga atgccaacaa tatcatcttc cttgaggata
     4261 agatgcttca catcattgaa ctccacctca gttcctgcgt atttggagta gataatttgt
     4321 gctccagtag ggacagtgat atcaattttg ttcttcccaa tagttcttcc ttcacccaca
     4381 gcaacgactt cacctccttg aggttttgat tgagcagtgg atggaagtaa gataccacct
     4441 aaagtcttct cctctgcctc cttgatcttc accaaaactc gatctcccaa tggcttaatt
     4501 gaagtatact taggggcaac aacagaggcc atatgtatat ctccttctta aagttaaaca
     4561 aaattatttc tagagggcaa ttgttatccg gtcgacgtcg accttagtat ccgtaacctg
     4621 agttgtccat tgggttgccg acgggaactg gttcaggttc cttgatctca acaacaacgc
     4681 agtccgacat caagaaagtc tttgcaaccg aagctgcgtg ttccaagcaa catctcacaa
     4741 cctttgttgg atcgatgatt cctgcagcca tcaaatcctc gtacttgccg gttgcagcat
     4801 tgtaaccgaa tttcacgtta tcgttggaaa gcaccttctc gctaactaca cttccattaa
     4861 caccagcatt cttggcgatt agttttaggg ggtaacttag tgctctttta actatatccg
     4921 ctccaacctt ttcttcatca ttatcaagcg tggctttaat ggcatcaacc ttggaagcaa
     4981 gacgaagcag agtgcaacca ccaccaacaa caataccttc ctcgacagcg gcctttgttg
     5041 cattaagagc atcttcaact ctcaacttct tctctttgag ttctgtctca gtttgtgctc
     5101 caacctgaat cacagcaact ccaccagaga gctttgcaat tctctcattg agtttctcct
     5161 tctcgtaatc ttgctccgct tgctcaataa gatttttaat ctgtgtaaca cgctttttca
     5221 ctgcatcctg tgtgcttcca tcacccacta tggtcgaggt ttcctttgtg aggacaacct
     5281 ttgaagcatt accaagaacc tcttttccag ctttgtcaag tgaaagacca acttcctcac
     5341 ggatcacagt tgctccagtt agaatggcaa tatcatcaag gtattggctc ttgcgctctc
     5401 caaaccctgg agctctgaga gctgctatct tcagtgtacc tctaagcttg tttacaacaa
     5461 gagtcgctaa agcttcttgt tcaatgtctt cagcaataat taatattggg tatccgcctc
     5521 taattgcatc ctccaaaaca ccaacaagat cccttgcatt ggtaattttc ttgtcaacga
     5581 gaagtaactt gcaattgtcg aactcgacgg acattttctc gctgtctgtc acaaagtaag
     5641 gagagatata accacggtca aattgcattc cttctacgac gtagaggttg ttctcggcac
     5701 ttttaccctc ctcaagggta accacacctt tcctgcccac tttgctcatt gcttcagcaa
     5761 tcatatttcc aatttcgtcg ttgttacccg cactaacagc tgcaacatct gcaagttcac
     5821 tgtcttcaac ctccttagac attttcttga gctcggtaac caaagccttt gctgtcttct
     5881 caatacctct ggtgatcaaa acagggtttg caccagcagc taccaccttg acaccctcag
     5941 cgataaaacc ttgtgcaaga acaacagatg ttgttgtacc gtcaccagcc aagtcattgg
     6001 ttttggcagc cgcttgcctc acaagcttag cgccaatgtt ctcaaccggg tcttccaact
     6061 caacctctct tgcaacagtc acaccatcat taacaattcg tggtgatcca tacttgctct
     6121 caagaacaac atttcgtcct ttgggtccaa gtgtaacgcc aacaagatct gcgagcttgt
     6181 tgacaccagc ttgaagtctc ctaatggtag tcccatcttt gttgaaatgt aattcctttg
     6241 ctgccatatg tatatctcct tcttaaagtt aaacaaaatt atttctagct ccttacacca
     6301 tgaggccctc aggagcagca gcagcaggag ccttgggttt cggtttgtca acaacaatgg
     6361 cctgagtggt cagtaccatt cctgctacgg aagcagcgtt ctgtagcgca catcttgtca
     6421 ctttagccgg atcaatcact ccagcttcga acagattctc gtaagtatca gtcattgcgt
     6481 tgtacccatt ctcccaatcg ctgaacataa tcttttccac aacaacttct ccttcaactc
     6541 cagcgttctg agcaataaga gcagctggtg acagcaatgc cttttgtact atgtcagctc
     6601 ccaaacgttc atcagcatcc tcaaaagtct ccttaatggc gggaataaca gtggagagat
     6661 gcaccaaagc agcaccacca cctggaacta tgccttcctc gatagcagca aatgttgcgt
     6721 tctttgcatc ctcgatacga agcttacggt cctcaagctc agtttcagtt gctgctccga
     6781 ctttaatgac agcaacacct ccagatagct tcgcaattct ctcagcgagc ttctctgagt
     6841 catacacaga atcagtctca aatagttctt tcttcagctg agcaatccga gcttgtaatt
     6901 cgtccttgga agctgcatct gcaatgaggg tagtcgaatc tttgctaatc gtgacttttc
     6961 gggcaatacc caattgatct atggttgcgt tttcaaccag taagctcatg tccatggcta
     7021 ggtactcagc tcctgtcaag attgcaatat cttgaagcat ggcttttctt ctttctccaa
     7081 atcctggcgc tttaacggca acaacattga ggacaccacg aagcttgttc acgacaaggg
     7141 tagctaaggc ttcaccagta acatcctctg caataatcaa caacggagca cgaagctgag
     7201 tggtcttctc caaaataggg atgatgtctt tgatcgcagt gatcttctga tcagtgatca
     7261 ataccctagc attctcaaac tcagctagta gtttctcagg gtttgtaaca aactgaggcg
     7321 agatgtaacc tctgtcaatc tccatccctt cttcaacttc gaccgtggtt tcaaaagaag
     7381 atgaagattc aatggacaaa acaccatcag gtccaacttt gtcgatggca tcagcaatca
     7441 ttgaccctat aaggtcatca tttccagcag agatagaagc cacagctctg atgtcatcac
     7501 gacctttcac gggcctagct ttcttctgaa gctcttcgat cagaccttga acagtcttat
     7561 caattcccct cttgagtgaa acgggattcg caccagaagt gacgctcaac aatccatgtt
     7621 tgattatctc ccgagcaagg atagacgcag tggttgtccc atcaccagca gagtcattag
     7681 tcttactcgc aacctcacga ataagcgctg caccagcatt ttccatggca ttaggtaact
     7741 caatagctct agcaatggtg actccatcat tcacaacctt aggacttcca aattcatcca
     7801 acacaacatt cctccctctg gggccaagcg taagaccaac acaatcagca agcttatcaa
     7861 taccagcttg tagagcagct ctagagtgct ggtcaaaagc tatttccttt acattcatat
     7921 gtatatctcc tactgcacct attgttggtg aagtgctcgt gaaaacacct aaacggactg
     7981 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacattatac gagccgatga
     8041 ttaattgtca acagctcatt tcagaatatt tgccagaacc gttatgatgt cggcgcaaaa
     8101 aacattatcc agaacgggag tgcgccttga gcgacacgaa ttatgcagtg atttacgacc
     8161 tgcacagcca taccacagct tccgatggct gcctgacgcc agaagcattg gtgcaccgtg
     8221 cagtcgatga taagctgtca aacatgagaa ttgtgcctaa tgagtgagct aacttacatt
     8281 aattgcgttg cgcgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg
     8341 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc
     8401 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
     8461 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
     8521 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag
     8581 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
     8641 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
     8701 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
     8761 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt
     8821 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg
     8881 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag
     8941 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat
     9001 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat
     9061 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc
     9121 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt
     9181 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa
     9241 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     9301 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct
     9361 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     9421 acgacaggtt tcccgactgg aaagcgggca gtgaagggtc cctaagtctc ctcagcaaaa
     9481 cgaaaggccc agtctttcga ctgagccttt cgttttattt gaccggatgt cctcttcctc
     9541 ctgggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc tagagcggtt
     9601 cagtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atcttttgcc
     9661 ctgtaaacga aaaaaccacc tggggaggtg gtttgatcga aggttaagtc agttggggaa
     9721 ctgcttaacc gtggtaactg gctttcgcag agcacagcaa ccaaatctgt ccttccagtg
     9781 tagccggact ttggcgcaca cttcaagagc aaccgcgtgt ttagctaaac aaatcctctg
     9841 cgaactccca gttaccaatg gctgctgcca gtggcgtttt accgtgcttt tccgggttgg
     9901 actcaagtga acagttaccg gataaggcgc agcagtcggg ctgaacgggg agttcttgct
     9961 tacagcccag cttggagcga acgacctaca ccgagccgag ataccagtgt gtgagctatg
    10021 agaaagcgcc acacttcccg taagggagaa aggcggaaca ggtatccggt aaacggcagg
    10081 gtcggaacag gagagcgcaa gagggagcga cccgccggaa acggtgggga tctttaagtc
    10141 ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc ctcacggctc ccacagatgc
    10201 accggaaaag cgtctgttta tgtgaactct ggcaggaggg cggagcctat ggaaaaacgc
    10261 caccggcgcg gccctgctgt tttgcctcac atgttagtcc cctgcttatc cacggaatct
    10321 gtgggtaact ttgtatgtgt ccgcagcgcc cgccgcagtc tcacgcccgg agcgtagcga
    10381 ccgagtgacg gccttaattt ggctt
//
