LOCUS       sequence-490604-       10399 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  different combination of A. thaliana and N. tabacum Rubisco assembly
            factors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Li S, Lee B, Lao Y, Lertwiriyapiti S, Huang X, Wang T
  TITLE     Directed evolution of a plant Rubisco chaperone with altered client 
            recognition.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Sep 16;122(37):e2510701122. doi: 
            10.1073/pnas.2510701122. Epub 2025 Sep 11.
  PUBMED    40932770
REFERENCE   2  (bases 1 to 10399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-09-16"; volume: "122(37):e2510701122. 
            doi"; pages: " 10.1073/pnas.2510701122. Epub 2025 Sep 11"
COMMENT     Sequence Label: pTW411c
FEATURES             Location/Qualifiers
     source          1..10399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             86..877
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        join(1194..1199,1200..1215,1216..1222)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1230..1246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4534..4556
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             5823..5831
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             6249..6271
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    7993..8009
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8017..8023,8024..8039,8040..8045))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        8288..8365
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8366..9448
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8385..8404)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      9605..10343
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        join(10393..10399,1..85)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
       61 tcaataatat tgaaaaagga agagtatgag ggaagcggtg atcgccgaag tatcgactca
      121 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      181 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      241 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      301 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      361 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      421 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      481 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      541 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      601 atggaactcg ccgcccgact gggctggaga tgagcgaaat gtagtgctta cgttgtcccg
      661 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
      721 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
      781 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
      841 cgtgaaaggc gagatcacca aggtagtcgg caaataaacg ccatgggcat taaaaaatgc
      901 cctcttgggt tatcaagagg gtcattatat ttgctgagga gtgccgttaa tgcgcaacgc
      961 aattaatgta agttagctca ctcattaggc acaattctca tgtttgacag cttatcatcg
     1021 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg
     1081 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa
     1141 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa
     1201 ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     1261 cagccagtcc gtttaggtgt tttcacgagc acttcaccaa caacgcgtac aactaggaga
     1321 tataccatgg gtatgcaaca actttatcag ccttttagac ctcctcctcc acctctccca
     1381 cccaaattcc gcaatcttga cacaaatgca aagctcgaag ttctatctaa tcgccttggt
     1441 ctttggtatg aatatgctcc tttgatacct tatttaacaa gtgaagggtt tactccttca
     1501 actcttgagg aaatcactgg ccttactgga gttgaacaga atcggttggt tgttgctgcc
     1561 caagttcgtg atactcttgt tgagtctgct gctttagatg aagaaacgtt gtcgtatttt
     1621 gaatctggag gtgctgaatt attatatgaa attcgtttgc ttagtgctag gcaacgaact
     1681 gatgcagcaa catttttagt gaagaatggg tttgatgcaa aacaagctca agatttagct
     1741 agagcaatta aagattatcc gcgtagacgt gtggattatg gttgggacaa gtttaatggt
     1801 gattctcctg gtgattgttt agcttttatg tactttagat tagctcaaga atatgctgcg
     1861 gcagcttctg aggatttgag gaggtcgtca atggagaagg cattagaagt tgttgagtct
     1921 gaaagtgcga ggaatttgct tgtgatggag ctagaaggac gtgaggttgc taaggagagt
     1981 gtacttgatg atggggtgac agtgcctttg gtgagaatga agttagggga ggtagctgag
     2041 tctacgattg tagtcgtttt gcctgtttgt aaggcggagg gaagggacgt ggaggtggag
     2101 gcggcgccgt gggaatgtgg tggcgtaggg gattttggta tagtggaggc ggagaaggat
     2161 tggaggagat gggttgtttt gcccggatgg caacctatcg cggggttaga gagaggtgga
     2221 gttgcagtgt cgttcaagag cgggaacttt ttgccgtgga gggagaagag taagtacaag
     2281 caagagccgg ttttggtagt ggcggatagg ggaaggacag aagtggcgtc ggaggacggg
     2341 ttttacttgg tggttgatgg tggagacggg agcaatgagg agggattgaa agtggaaaga
     2401 gggtcaacat tgaagaaaag aggggttgaa cagagcttag gaattgtgct tatagtagtt
     2461 aggccaccaa ggtgggaaga tgaagaacaa ttaggtgaag aagattggga ttaaattaca
     2521 aaaattagga gatatacata tgatgctatt gggcgatttt ggggcgagag atccattccc
     2581 agctgaaata gagagtaaat tcggtgaaaa agttttgggt aatactgaca cggagcacaa
     2641 aattctcatc ccagctgctt ctgctctgtc ccttgctcag caagactgca ttgtcatctc
     2701 acctggtcaa actcctctct cggaatatga agctcgacag ctcctattca aggttgttgg
     2761 ctggagactg gcacatgaag atggggtgct caagctacaa tgtacatgga aattaagaga
     2821 cttcgaatgt ggggttgaac ttatcaatcg aataggcaaa gtggtggaag gcactgggca
     2881 cgttcccaca cttcttcact tagagcagtc caatcaagtt cgtgctgagc tatggactgc
     2941 ttccatcgga ggtttgagca taaacgattt cattgtagct gctaaaatag atcagataaa
     3001 gacatcggac cttgtcccaa gaaaaagagt ttgggcataa cggctgaaat taatgaggag
     3061 atatacatat ggcaaaagca tttaagggta attacaagaa tttaaagaag gagaaaaaga
     3121 gtaggaggaa tcttgtggtt gtcagtgatt taggtggaca gtatgaatat ggctttgatg
     3181 atatcaaaac gcaaattctt aactacttca catttaaagc agtgaggact attttgaacc
     3241 agcttaatga aatggactct ccacaatatc aatggttcaa tgattatgtt acagtaaata
     3301 agcttaggga tgggaagcgt tttattcgca accttgctaa ggagaagcaa gaactcgctg
     3361 aaaaagtgat ggtaacacgg ctcagtctgt atgcgaaatg gataaaaaaa tgtgatcacg
     3421 aggagatcta caaccgaata tctgatcaga atgtggaagt gatgcgtgag cggctcatgg
     3481 aaactgtgat ttggccatca gatgacacga actcggagag agttgggtaa ttcagctaag
     3541 acactgcaca ggagatatac atatgatggc cgcaaacaat aatcctcagg gcactaaacc
     3601 aaacagttta gtttgtgcaa attgtgaagg ggaaggttgt gtggcatgtt ctcaatgcaa
     3661 aggaggtggt gtgaatttga ttgatcattt caatggtcag tttaaagctg gtgcactctg
     3721 ttggctttgc agaggtaaaa aggaggtgtt gtgtggggac tgcaatggag ctggtttcat
     3781 tggtgggttc ttgagcacct tcgatgagta gagtcagtag ggccctaaaa aaaaaccccg
     3841 cccctgacag ggcagggttt tttttggcga aaaaaccccg ctgaagcggg gttttttgcg
     3901 tacattggct ccgtctaaga aagtatagcc atcacatctg aagctctgag ggcaatgtag
     3961 ttggaaccat ctttgccctt gaagtcgtta ccagcatact tggagtaaag tactgtgctt
     4021 ccggttgata ctggtagagg cgtaatttta ccttcctcgt ctagggaacc cggtccaact
     4081 gctatcacgg tgccaataga aggcttctct ttggtagtct cggttaacaa caaccctcca
     4141 gctgttttct cctccgcctc agcaacctta ataaagactc ggtcattcaa aggtttgaga
     4201 tctttgatgt cctctgtctc aagaatgcca acaatatcat cttccttgag gataagatgc
     4261 ttcacatcat tgaactccac ctcagttcct gcgtatttgg agtagataat ttgtgctcca
     4321 gtagggacag tgatatcaat tttgttcttc ccaatagttc ttccttcacc cacagcaacg
     4381 acttcacctc cttgaggttt tgattgagca gtggatggaa gtaagatacc acctaaagtc
     4441 ttctcctctg cctccttgat cttcaccaaa actcgatctc ccaatggctt aattgaagta
     4501 tacttagggg caacaacaga ggccatatgt atatctcctt cttaaagtta aacaaaatta
     4561 tttctagagg gcaattgtta tccggtcgac gtcgacctta gtatccgtaa cctgagttgt
     4621 ccattgggtt gccgacggga actggttcag gttccttgat ctcaacaaca acgcagtccg
     4681 acatcaagaa agtctttgca accgaagctg cgtgttccaa gcaacatctc acaacctttg
     4741 ttggatcgat gattcctgca gccatcaaat cctcgtactt gccggttgca gcattgtaac
     4801 cgaatttcac gttatcgttg gaaagcacct tctcgctaac tacacttcca ttaacaccag
     4861 cattcttggc gattagtttt agggggtaac ttagtgctct tttaactata tccgctccaa
     4921 ccttttcttc atcattatca agcgtggctt taatggcatc aaccttggaa gcaagacgaa
     4981 gcagagtgca accaccacca acaacaatac cttcctcgac agcggccttt gttgcattaa
     5041 gagcatcttc aactctcaac ttcttctctt tgagttctgt ctcagtttgt gctccaacct
     5101 gaatcacagc aactccacca gagagctttg caattctctc attgagtttc tccttctcgt
     5161 aatcttgctc cgcttgctca ataagatttt taatctgtgt aacacgcttt ttcactgcat
     5221 cctgtgtgct tccatcaccc actatggtcg aggtttcctt tgtgaggaca acctttgaag
     5281 cattaccaag aacctctttt ccagctttgt caagtgaaag accaacttcc tcacggatca
     5341 cagttgctcc agttagaatg gcaatatcat caaggtattg gctcttgcgc tctccaaacc
     5401 ctggagctct gagagctgct atcttcagtg tacctctaag cttgtttaca acaagagtcg
     5461 ctaaagcttc ttgttcaatg tcttcagcaa taattaatat tgggtatccg cctctaattg
     5521 catcctccaa aacaccaaca agatcccttg cattggtaat tttcttgtca acgagaagta
     5581 acttgcaatt gtcgaactcg acggacattt tctcgctgtc tgtcacaaag taaggagaga
     5641 tataaccacg gtcaaattgc attccttcta cgacgtagag gttgttctcg gcacttttac
     5701 cctcctcaag ggtaaccaca cctttcctgc ccactttgct cattgcttca gcaatcatat
     5761 ttccaatttc gtcgttgtta cccgcactaa cagctgcaac atctgcaagt tcactgtctt
     5821 caacctcctt agacattttc ttgagctcgg taaccaaagc ctttgctgtc ttctcaatac
     5881 ctctggtgat caaaacaggg tttgcaccag cagctaccac cttgacaccc tcagcgataa
     5941 aaccttgtgc aagaacaaca gatgttgttg taccgtcacc agccaagtca ttggttttgg
     6001 cagccgcttg cctcacaagc ttagcgccaa tgttctcaac cgggtcttcc aactcaacct
     6061 ctcttgcaac agtcacacca tcattaacaa ttcgtggtga tccatacttg ctctcaagaa
     6121 caacatttcg tcctttgggt ccaagtgtaa cgccaacaag atctgcgagc ttgttgacac
     6181 cagcttgaag tctcctaatg gtagtcccat ctttgttgaa atgtaattcc tttgctgcca
     6241 tatgtatatc tccttcttaa agttaaacaa aattatttct agctccttac accatgaggc
     6301 cctcaggagc agcagcagca ggagccttgg gtttcggttt gtcaacaaca atggcctgag
     6361 tggtcagtac cattcctgct acggaagcag cgttctgtag cgcacatctt gtcactttag
     6421 ccggatcaat cactccagct tcgaacagat tctcgtaagt atcagtcatt gcgttgtacc
     6481 cattctccca atcgctgaac ataatctttt ccacaacaac ttctccttca actccagcgt
     6541 tctgagcaat aagagcagct ggtgacagca atgccttttg tactatgtca gctcccaaac
     6601 gttcatcagc atcctcaaaa gtctccttaa tggcgggaat aacagtggag agatgcacca
     6661 aagcagcacc accacctgga actatgcctt cctcgatagc agcaaatgtt gcgttctttg
     6721 catcctcgat acgaagctta cggtcctcaa gctcagtttc agttgctgct ccgactttaa
     6781 tgacagcaac acctccagat agcttcgcaa ttctctcagc gagcttctct gagtcataca
     6841 cagaatcagt ctcaaatagt tctttcttca gctgagcaat ccgagcttgt aattcgtcct
     6901 tggaagctgc atctgcaatg agggtagtcg aatctttgct aatcgtgact tttcgggcaa
     6961 tacccaattg atctatggtt gcgttttcaa ccagtaagct catgtccatg gctaggtact
     7021 cagctcctgt caagattgca atatcttgaa gcatggcttt tcttctttct ccaaatcctg
     7081 gcgctttaac ggcaacaaca ttgaggacac cacgaagctt gttcacgaca agggtagcta
     7141 aggcttcacc agtaacatcc tctgcaataa tcaacaacgg agcacgaagc tgagtggtct
     7201 tctccaaaat agggatgatg tctttgatcg cagtgatctt ctgatcagtg atcaataccc
     7261 tagcattctc aaactcagct agtagtttct cagggtttgt aacaaactga ggcgagatgt
     7321 aacctctgtc aatctccatc ccttcttcaa cttcgaccgt ggtttcaaaa gaagatgaag
     7381 attcaatgga caaaacacca tcaggtccaa ctttgtcgat ggcatcagca atcattgacc
     7441 ctataaggtc atcatttcca gcagagatag aagccacagc tctgatgtca tcacgacctt
     7501 tcacgggcct agctttcttc tgaagctctt cgatcagacc ttgaacagtc ttatcaattc
     7561 ccctcttgag tgaaacggga ttcgcaccag aagtgacgct caacaatcca tgtttgatta
     7621 tctcccgagc aaggatagac gcagtggttg tcccatcacc agcagagtca ttagtcttac
     7681 tcgcaacctc acgaataagc gctgcaccag cattttccat ggcattaggt aactcaatag
     7741 ctctagcaat ggtgactcca tcattcacaa ccttaggact tccaaattca tccaacacaa
     7801 cattcctccc tctggggcca agcgtaagac caacacaatc agcaagctta tcaataccag
     7861 cttgtagagc agctctagag tgctggtcaa aagctatttc ctttacattc atatgtatat
     7921 ctcctactgc acctattgtt ggtgaagtgc tcgtgaaaac acctaaacgg actggctgtt
     7981 tcctgtgtga aattgttatc cgctcacaat tccacacatt atacgagccg atgattaatt
     8041 gtcaacagct catttcagaa tatttgccag aaccgttatg atgtcggcgc aaaaaacatt
     8101 atccagaacg ggagtgcgcc ttgagcgaca cgaattatgc agtgatttac gacctgcaca
     8161 gccataccac agcttccgat ggctgcctga cgccagaagc attggtgcac cgtgcagtcg
     8221 atgataagct gtcaaacatg agaattgtgc ctaatgagtg agctaactta cattaattgc
     8281 gttgcgcgac accatcgaat ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga
     8341 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
     8401 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
     8461 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
     8521 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
     8581 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
     8641 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
     8701 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
     8761 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
     8821 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga
     8881 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
     8941 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
     9001 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
     9061 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
     9121 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
     9181 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga
     9241 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
     9301 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
     9361 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9421 ggtttcccga ctggaaagcg ggcagtgaag ggtccctaag tctcctcagc aaaacgaaag
     9481 gcccagtctt tcgactgagc ctttcgtttt atttgaccgg atgtcctctt cctcctgggc
     9541 tgacttcagg tgctacattt gaagagataa attgcactga aatctagagc ggttcagtag
     9601 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatcttt tgccctgtaa
     9661 acgaaaaaac cacctgggga ggtggtttga tcgaaggtta agtcagttgg ggaactgctt
     9721 aaccgtggta actggctttc gcagagcaca gcaaccaaat ctgtccttcc agtgtagccg
     9781 gactttggcg cacacttcaa gagcaaccgc gtgtttagct aaacaaatcc tctgcgaact
     9841 cccagttacc aatggctgct gccagtggcg ttttaccgtg cttttccggg ttggactcaa
     9901 gtgaacagtt accggataag gcgcagcagt cgggctgaac ggggagttct tgcttacagc
     9961 ccagcttgga gcgaacgacc tacaccgagc cgagatacca gtgtgtgagc tatgagaaag
    10021 cgccacactt cccgtaaggg agaaaggcgg aacaggtatc cggtaaacgg cagggtcgga
    10081 acaggagagc gcaagaggga gcgacccgcc ggaaacggtg gggatcttta agtcctgtcg
    10141 ggtttcgccc gtactgtcag attcatggtt gagcctcacg gctcccacag atgcaccgga
    10201 aaagcgtctg tttatgtgaa ctctggcagg agggcggagc ctatggaaaa acgccaccgg
    10261 cgcggccctg ctgttttgcc tcacatgtta gtcccctgct tatccacgga atctgtgggt
    10321 aactttgtat gtgtccgcag cgcccgccgc agtctcacgc ccggagcgta gcgaccgagt
    10381 gacggcctta atttggctt
//
