LOCUS       sequence-490658-        7266 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_001, derived from human fusion protein ABR_DUXA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7266)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_001
FEATURES             Location/Qualifiers
     source          1..7266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(799..1509,1510..1512,1513..1515))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(841..862)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1190..1209
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1449..1470
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1512..1521
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1597..1800)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1626..1646)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1801..2104
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2361..2405)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2590..2823
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3183..3300
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3792..3917
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3964..4221
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4223..4602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4633..4737
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4738..4806,4807..5598)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4956..4975)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5769..6357
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6258..6277
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6511..6528
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6596..6730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6620..6639)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6674..6693
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(6736..6931)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6750..6885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    6990..7011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7026..7031,7032..7049,7050..7056)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7064..7080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7069..7091
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7088..7104
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7088..7104
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca tgcctccacg ccgggtgcta acccttccgg
      601 cggcgtgggt gataccccat caccacctcc ttgagagcgg gccgaaagtt ggggactcat
      661 cgtaggtgac tcgtcaatgt aaggcattgt ctccgagcct tcaggaggcc ctttttgttc
      721 ctcatttccc tctccatcat actcatctgt accgtaccgg gaagcggccg cacttccccc
      781 ggagcctccg cttccgccct tgtacagctc gtccatgccg agagtgatcc cggcggcggt
      841 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga
      901 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
      961 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
     1021 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
     1081 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
     1141 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
     1201 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
     1261 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt
     1321 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
     1381 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
     1441 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
     1501 gcccttgctc accatggtgg cgaccggtgg atcccgggcc cgcggtaccg tcgactgcag
     1561 aattcggttc actaaacgag cccggttcac taaacgagct ctgcttatat agacctccca
     1621 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     1681 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     1741 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     1801 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     1861 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     1921 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     1981 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     2041 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     2101 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     2161 actagcacgc gttcgaagct tgagctcggt cccctcgggg ttgggaggtg ggtctgaaac
     2221 gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt
     2281 cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt
     2341 gttatgttaa accaattcca caaacttgcc catttatcta attccaataa ttcttgttca
     2401 ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg
     2461 ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta
     2521 ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc
     2581 caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg
     2641 agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc
     2701 agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc
     2761 gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct
     2821 cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct
     2881 actcctaatg gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc
     2941 ctcatatctc ctccttatgg tgttttacta aacttttcca tgttctaatc ctcatcctgt
     3001 ctacttgcca cacaatcatc acctgccatc tgttttccat aatccctaat gatctttgct
     3061 tttcttcttg gcactacttt tatgtcacta ttatcttgta ttactactgc cccttcacct
     3121 ttccagagga gctttgctgg tcctttccaa agtggatttc tgctgtccct gtaataaacc
     3181 cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct
     3241 attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa
     3301 ttgtggatga atactgccat ttgtactgct gtcttaagat gttcagcctg atctcttacc
     3361 tgtcctataa ttttctttaa ttctttattc atagattcta ctactccttg actttgggga
     3421 ttgtagggaa tttccaggtc tgaagatcct gctgcttgct gtgctttttt cttacttttg
     3481 ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga
     3541 tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc
     3601 tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg
     3661 ccagtattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta
     3721 tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc
     3781 cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt
     3841 tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga
     3901 gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc
     3961 cactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     4021 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     4081 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     4141 acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg ccactcccca
     4201 gtcccgccca ggccacgcct cccatggtaa tagcgatgac taatacgtag atgtactgcc
     4261 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     4321 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     4381 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     4441 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     4501 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     4561 ccgtaattga ttactattaa taactagtca ataatcaatg tcgacccagg tggcactttt
     4621 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4681 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4741 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4801 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4861 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4921 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4981 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5041 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5101 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5161 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5221 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5281 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5341 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5401 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5461 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5521 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5581 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5641 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5701 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5761 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5821 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5881 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5941 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6001 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6061 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6121 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6181 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6241 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6301 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6361 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6421 tttcctgcgt tatcccctgc ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6481 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     6541 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcggatcc
     6601 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6661 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     6721 taaacaagtt agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     6781 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     6841 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     6901 cgggactatg gttgctgact aattgagatg cggatccgct ggcacgacag gtttcccgac
     6961 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7021 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7081 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgggctgca ggtcgaggga
     7141 tctccataag agaagaggga cagctatgac tgggagtagt caggagagga ggaaaaatct
     7201 ggctagtaaa acatgtaagg aaaattttag ggatgttaaa gaaaaaaata acacaaaaca
     7261 aaatat
//
