LOCUS       sequence-490663-        7677 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_023, derived from human fusion protein BRD9_NUTM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7677)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_023
FEATURES             Location/Qualifiers
     source          1..7677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1210..1920,1921..1923,1924..1926))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1252..1273)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1601..1620
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1860..1881
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1923..1932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2008..2211)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2037..2057)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2212..2515
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2772..2816)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3001..3234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3594..3711
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4203..4328
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4375..4632
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4634..5013
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5044..5148
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5149..5217,5218..6009)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5367..5386)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6180..6768
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6669..6688
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6922..6939
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7007..7141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7031..7050)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7085..7104
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7147..7342)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7161..7296
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7401..7422
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7437..7442,7443..7460,7461..7467)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7475..7491
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7480..7502
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7499..7515
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7499..7515
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca ggagcggtca cctagggaag gcttggatag
      601 agtggccaca ggacctcctt ccccggttgt cccatgtggc cctggccaag ctttttccag
      661 gggcacaatg gggaccagtt gagcaactgg tggtggaggc tgaggcggaa ggcctggagg
      721 accctcctgg ctgacaccaa cagccttaga gggcagaatg gtcttcacat tagatgctgt
      781 cacaaatgga ggtgcaggac cctcaaggcc tccacaggga gtgcccgggg cagtcgaatt
      841 gagggcagtc tgagtaagga taaagttctg agtttgagag ggctcagctg accccccttc
      901 tgtcttgact ttgacaatga ccttgccagc cccagcccca ctgaggcaag ggcccccgtc
      961 ccctgtcacc aacagtgagc tggggaaagc agagagcatc agagggttgt ctggagagaa
     1021 tactgaaggc atgatgggct gtggaggtgg ctccctgggt gggtggtctg gtgggtcaga
     1081 agttggtggg agaaagggaa gtgcagggga tggagacagg gcggcactag gtttcatgct
     1141 catatccggt cccggcaatg cagatgcccg ggaagcggcc gcacttcccc cggagcctcc
     1201 gcttccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
     1261 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg actgggtgct
     1321 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
     1381 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt
     1441 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
     1501 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
     1561 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
     1621 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
     1681 gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt
     1741 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
     1801 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
     1861 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
     1921 caccatggtg gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca gaattcggtt
     1981 cactaaacga gcccggttca ctaaacgagc tctgcttata tagacctccc accgtacacg
     2041 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
     2101 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
     2161 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
     2221 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg
     2281 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat
     2341 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg
     2401 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg
     2461 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa
     2521 ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagcacg
     2581 cgttcgaagc ttgagctcgg tcccctcggg gttgggaggt gggtctgaaa cgataatggt
     2641 gaatatccct gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct
     2701 accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta
     2761 aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc attcttttct
     2821 tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct
     2881 ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa
     2941 ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc
     3001 aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg
     3061 atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg
     3121 ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac
     3181 gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc
     3241 actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat
     3301 ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct
     3361 cctccttatg gtgttttact aaacttttcc atgttctaat cctcatcctg tctacttgcc
     3421 acacaatcat cacctgccat ctgttttcca taatccctaa tgatctttgc ttttcttctt
     3481 ggcactactt ttatgtcact attatcttgt attactactg ccccttcacc tttccagagg
     3541 agctttgctg gtcctttcca aagtggattt ctgctgtccc tgtaataaac ccgaaaattt
     3601 tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct
     3661 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg
     3721 aatactgcca tttgtactgc tgtcttaaga tgttcagcct gatctcttac ctgtcctata
     3781 attttcttta attctttatt catagattct actactcctt gactttgggg attgtaggga
     3841 atttccaggt ctgaagatcc tgctgcttgc tgtgcttttt tcttactttt gttttgctct
     3901 tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa
     3961 tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat
     4021 ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag gccagtatta
     4081 actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt atattttttc
     4141 tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata
     4201 ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta
     4261 ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt
     4321 cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag
     4381 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
     4441 gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt
     4501 tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac
     4561 aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc agtcccgccc
     4621 aggccacgcc tcccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     4681 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     4741 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     4801 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     4861 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     4921 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     4981 attactatta ataactagtc aataatcaat gtcgacccag gtggcacttt tcggggaaat
     5041 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5101 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5161 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5221 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5281 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5341 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5401 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5461 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5521 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5581 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5641 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5701 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5761 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5821 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5881 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5941 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6001 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6061 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6121 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6181 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6241 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6301 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6361 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6421 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6481 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6541 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6601 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6661 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6721 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6781 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6841 ttatcccctg cttctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6901 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6961 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcggatc cagacatgat
     7021 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     7081 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     7141 tagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     7201 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     7261 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     7321 ggttgctgac taattgagat gcggatccgc tggcacgaca ggtttcccga ctggaaagcg
     7381 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7441 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7501 ggaaacagct atgaccatga ttacgccaag cttgggctgc aggtcgaggg atctccataa
     7561 gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc tggctagtaa
     7621 aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac aaaatat
//
