LOCUS       sequence-490721-        7854 bp    DNA     circular SYN 27-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_062, derived from human fusion protein ETV6_C7ORF72.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7854)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 27, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_062
FEATURES             Location/Qualifiers
     source          1..7854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1387..2097,2098..2100,2101..2103))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1429..1450)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1778..1797
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2037..2058
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2100..2109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2185..2388)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2214..2234)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2389..2692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2949..2993)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3178..3411
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3771..3888
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4380..4505
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4552..4809
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4811..5190
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5221..5325
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5326..5394,5395..6186)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5544..5563)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6357..6945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6846..6865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7099..7116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7184..7318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7208..7227)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7262..7281
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7324..7519)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7338..7473
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7578..7599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7614..7619,7620..7637,7638..7644)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7652..7668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7657..7679
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7676..7692
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7676..7692
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca ataatcaatg gtttctttgt ctttcttgtt
      601 aaagggatta taaggacgca cggtagtgac taaacgcgac aatggggctt gatgacgaaa
      661 caggtctacg gccatctcct tttgaaatac acgatgtaac tcggaatccg gcgacggtgt
      721 ggtagatggg atgttagagt tcgctggatg tgtggcagtg aaatgcactt tccccgtgta
      781 acccggaatg ttcgctgcac ggctagtgtt agtatataac ggtttcgagt agcgctgttt
      841 ggctaaggac gcaatgtcgc ccagcggatt gtctacgtcg tccgcgtttg tactttgaac
      901 gtggcccgtg tataacggaa tgtacttgct acggctactc gatgatacaa aattgatggg
      961 tttaacaggc gcaagaaaac tacgtgtctg aaaacgatcc cataactcac ccaccatctg
     1021 agtaatggct ggtactccct tatagcgttt taacgtaaaa cactggctga tggggtcggc
     1081 ggtcttctct aaagtagtct cttctgggac cagcttcggc ggaagcattt tatcccacgg
     1141 aacgtcttcg tatgctttct gtgtcgagga tttataaaag ctatctttgg cattacactt
     1201 tttgttggtc tgtaccagag gctctccttc gtcggtgcat gtggcttctt catcaaaagt
     1261 aaagatgtga gaaagggaac gtccctcatg atgaggttgc agtggagtca ctttaagggg
     1321 actagtccac ccactatgag gggaccggga agcggccgca cttcccccgg agcctccgct
     1381 tccgcccttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
     1441 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agcttggact gggtgctcag
     1501 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
     1561 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
     1621 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
     1681 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
     1741 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
     1801 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
     1861 ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac
     1921 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
     1981 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc
     2041 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
     2101 catggtggcg accggtggat cccgggcccg cggtaccgtc gactgcagaa ttcggttcac
     2161 taaacgagcc cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct
     2221 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
     2281 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
     2341 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
     2401 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
     2461 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
     2521 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
     2581 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
     2641 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc
     2701 catatatggg ctatgaacta atgaccccgt aattgattac tattaataac tagcacgcgt
     2761 tcgaagcttg agctcggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa
     2821 tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct taaacctacc
     2881 aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac
     2941 caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc
     3001 tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg
     3061 tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta
     3121 gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg
     3181 agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg
     3241 ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc
     3301 tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct
     3361 gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc tattcccact
     3421 gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac tcctaatggt
     3481 tcaattttta ctactttata tttatataat tcacttctcc aattgtccct catatctcct
     3541 ccttatggtg ttttactaaa cttttccatg ttctaatcct catcctgtct acttgccaca
     3601 caatcatcac ctgccatctg ttttccataa tccctaatga tctttgcttt tcttcttggc
     3661 actactttta tgtcactatt atcttgtatt actactgccc cttcaccttt ccagaggagc
     3721 tttgctggtc ctttccaaag tggatttctg ctgtccctgt aataaacccg aaaattttga
     3781 atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact
     3841 attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat
     3901 actgccattt gtactgctgt cttaagatgt tcagcctgat ctcttacctg tcctataatt
     3961 ttctttaatt ctttattcat agattctact actccttgac tttggggatt gtagggaatt
     4021 tccaggtctg aagatcctgc tgcttgctgt gcttttttct tacttttgtt ttgctcttcc
     4081 tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag
     4141 agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt
     4201 tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc agtattaact
     4261 gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt
     4321 ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg
     4381 acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc
     4441 accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga
     4501 gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga
     4561 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
     4621 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
     4681 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg
     4741 caaaaagcag ctgcttatat gcaggatctg agggctcgcc actccccagt cccgcccagg
     4801 ccacgcctcc catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     4861 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     4921 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     4981 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     5041 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     5101 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     5161 actattaata actagtcaat aatcaatgtc gacccaggtg gcacttttcg gggaaatgtg
     5221 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5281 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5341 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5401 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5461 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5521 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5581 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5641 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5701 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5761 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5821 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5881 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5941 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6001 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6061 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6121 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6181 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6241 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6301 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6361 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6421 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6481 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6541 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6601 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6661 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6721 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6781 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6841 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6901 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6961 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7021 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7081 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7141 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcggatccag acatgataag
     7201 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     7261 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttag
     7321 ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     7381 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     7441 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     7501 tgctgactaa ttgagatgcg gatccgctgg cacgacaggt ttcccgactg gaaagcgggc
     7561 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7621 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7681 aacagctatg accatgatta cgccaagctt gggctgcagg tcgagggatc tccataagag
     7741 aagagggaca gctatgactg ggagtagtca ggagaggagg aaaaatctgg ctagtaaaac
     7801 atgtaaggaa aattttaggg atgttaaaga aaaaaataac acaaaacaaa atat
//
