LOCUS       sequence-490726-        8362 bp    DNA     circular SYN 27-OCT-2025
DEFINITION  AAV vector expressing both SOD2 and mitochondrial targeted Catalase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Xiong W, MacColl Garfinkel AE, Li Y, Benowitz LI, Cepko CL
  TITLE     NRF2 promotes neuronal survival in neurodegeneration and acute nerve
            damage.
  JOURNAL   J Clin Invest. 2015 Apr;125(4):1433-45. doi: 10.1172/JCI79735. Epub 
            2015 Mar 23.
  PUBMED    25798616
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 27, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI79735"; 
            journalName: "J Clin Invest"; date: "2015-04"; volume: "125"; issue:
            "4"; pages: "1433-45"
COMMENT     Sequence Label: AAV-CMV-SOD2-2A-Catalase-WPRE
FEATURES             Location/Qualifiers
     source          1..8362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(569..588)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     688..710
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(748..766)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        834..938
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(939..1007,1008..1799)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1157..1176)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1970..2558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2459..2478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2850..3229
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3230..3433
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3384..3404
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3430..3454
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3567..4042
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             4777..4830
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(6535..6555)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6953..6964)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7031..7048)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7037..7244
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7436..7565
                     /label=AAV2 ITR
     rep_origin      join(7936..8362,1..87)
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8023..8042)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8233..8254
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttaacaaa atattaacgc ttacaattta aatatttgct tatacaatct tcctgttttt
       61 ggggcttttc tgattatcaa ccggggtaca tatgattgac atgctagttt tacgattacc
      121 gttcatcgat tctcttgttt gctccagact ctcaggcaat gacctgatag cctttgtaga
      181 gacctctcaa aaatagctac cctctccggc atgaatttat cagctagaac ggttgaatat
      241 catattgatg gtgatttgac tgtctccggc ctttctcacc cgtttgaatc tttacctaca
      301 cattactcag gcattgcatt taaaatatat gagggttcta aaaattttta tccttgcgtt
      361 gaaataaagg cttctcccgc aaaagtatta cagggtcata atgtttttgg tacaaccgat
      421 ttagctttat gctctgaggc tttattgctt aattttgcta attctttgcc ttgcctgtat
      481 gatttattgg atgttggaat cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
      541 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
      601 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
      661 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
      721 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
      781 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
      841 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      901 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      961 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1021 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1081 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1141 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1201 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1261 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1321 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1381 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1441 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1501 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1561 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1621 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1681 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1741 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1801 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1861 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1921 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1981 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2041 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2101 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2161 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2221 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2281 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2341 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2401 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2461 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2521 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2581 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2641 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2701 cgaccgagcg cagcgagtca gtgagcgagc gcgcagagag ggagtggcca actccatcac
     2761 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt acatttatat
     2821 tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta
     2881 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2941 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     3001 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     3061 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     3121 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     3181 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     3241 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     3301 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     3361 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     3421 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     3481 tgacctccat agaagacacc gggaccgatc cagcctcccc tcgaagctta catgtggtac
     3541 cgagctcgga tcctgagaac ttcagggtga gtctatggga cccttgatgt tttctttccc
     3601 cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg gtacacatat
     3661 tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt cttttaatat
     3721 acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca gggcaataat
     3781 gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata atttctgggt
     3841 taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt aactgatgta
     3901 agaggtttca tattgctaat agcagctaca atccagctac cattctgctt ttattttatg
     3961 gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa tcatgttcat
     4021 acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc tggcccatca
     4081 ctttggcaaa gaattccgcg ggcggccgcc atgttgtgtc gggcggcgtg cagcacgggc
     4141 aggaggctgg gccctgtggc cggtgccgcg ggctcccggc acaagcacag cctcccagac
     4201 ctgccttacg actatggcgc gctggagcca cacattaacg cgcagatcat gcagctgcac
     4261 cacagcaagc accacgcggc ctacgtgaac aatctcaacg ccaccgagga gaagtaccac
     4321 gaggctctgg ccaagggaga tgttacaact caggtcgctc ttcagcctgc actgaagttc
     4381 aatggtgggg gacatattaa tcacaccatt ttctggacaa acctgagccc taagggtggt
     4441 ggagaaccca aaggagagtt gctggaggct atcaagcgtg actttgggtc ttttgagaag
     4501 tttaaggaga agctgacagc cgtgtctgtg ggagtccaag gttcaggctg gggctggctt
     4561 ggcttcaata aggagcaagg tcgcttacag attgctgcct gctctaatca ggacccattg
     4621 caaggaacaa caggccttat tccgctgctg gggattgacg tgtgggagca cgcttactac
     4681 cttcagtata aaaacgtcag acctgactat ctgaaagcta tttggaatgt aatcaactgg
     4741 gagaatgtta ctgaaagata cacagcttgc aagaaggagg gcagaggaag tctgctaaca
     4801 tgcggtgacg tcgaggagaa tcctggccca atgctgttta atctgaggat cctgttaaac
     4861 aatgcagctt ttagaaatgg tcacaacttc atggttcgaa attttcggtg tggacaacca
     4921 ctacaatcgg acagtcggga cccagccagc gaccagatga agcagtggaa ggagcagcgg
     4981 gcctcgcaga gacctgatgt cctgaccacc ggaggcggga acccaatagg agataaactt
     5041 aatatcatga ccgcggggtc ccgagggccc ctcctcgttc aggatgtggt tttcactgac
     5101 gagatggcac actttgacag agagcggatt cctgagagag tggtacacgc aaaaggagca
     5161 ggtgcttttg gatactttga ggtcacccac gatatcacca gatactccaa ggcaaaggtg
     5221 tttgagcata ttggaaagag gacccctatt gccgttcgat tctccacagt cgctggagag
     5281 tcaggctcag ctgacacagt tcgtgaccct cgggggtttg cagtgaaatt ttacactgaa
     5341 gatggtaact gggatcttgt gggaaacaac acccctattt tcttcatcag ggatgccata
     5401 ttgtttccat cctttatcca tagccagaag agaaacccac agactcacct gaaggatcct
     5461 gacatggtct gggacttctg gagtcttcgt cccgagtctc tccatcaggt ttctttcttg
     5521 ttcagtgacc gagggattcc cgatggtcac cggcacatga atggctatgg atcacacacc
     5581 ttcaagttgg ttaatgcaga tggagaggca gtctattgca agttccatta caagaccgac
     5641 cagggcatca aaaacttgcc tgttggagag gcaggaaggc ttgctcagga agatccggat
     5701 tatggcctcc gagatctttt caatgccatc gccaatggca attacccgtc ctggacgttt
     5761 tacatccagg tcatgacttt taaggaggca gaaactttcc catttaatcc atttgatctg
     5821 accaaggttt ggcctcacaa ggactaccct cttataccag ttggcaaact ggttttaaac
     5881 aaaaatccag ttaattactt tgctgaagtt gaacagatgg cttttgaccc aagcaatatg
     5941 ccccctggca tcgagcccag ccctgacaaa atgcttcagg gccgcctttt tgcctacccg
     6001 gacactcacc gccaccgcct gggacccaac tatctgcaga tacctgtgaa ctgtccctac
     6061 cgcgctcgag tggccaacta ccagcgtgat ggccccatgt gcatgcatga caaccagggt
     6121 ggtgccccca actattaccc caacagcttc agcgcaccag agcagcagcg ctcagccctg
     6181 gagcacagcg tccagtgcgc tgtagatgtg aaacgcttca acagtgctaa tgaagacaat
     6241 gtcactcagg tgcggacatt ctacacaaag gtgttgaacg aggaggagag gaaacgcctg
     6301 tgtgagaaca ttgccggcca cctgaaggac gctcagcttt tcattcagaa gaaagcggtc
     6361 aagaatttca ctgacgtcca ccctgactat ggggcccgca tccaggctct tctggacaag
     6421 tacaacgctg agaagcctaa gaacgcaatt cacacctaca cgcaggccta agcttggatc
     6481 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6541 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6601 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6661 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6721 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6781 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6841 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     6901 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6961 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7021 tcgagatctg cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7081 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7141 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7201 agcaaggggg aggattggga agacaatagc aggcatgctg gggactcgag tgctttattt
     7261 gtgaaatttg tgatactatt gctttatttg taaccattat aagctgcaat aaacaagtta
     7321 acaacaacaa ttgcattcat tttattgttt caggttcagg gggaggtgtg ggaggttttt
     7381 taaaggggga gggggtacgt agataagtag catggcgggt taatcattaa ctacaaggaa
     7441 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     7501 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     7561 cgcagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     7621 ctgaatggcg aatggcgatt ccgttgcaat ggctggcggt aatattgttc tggatattac
     7681 cagcaaggcc gatagtttga gttcttctac tcaggcaagt gatgttatta ctaatcaaag
     7741 aagtattgcg acaacggtta atttgcgtga tggacagact cttttactcg gtggcctcac
     7801 tgattataaa aacacttctc aggattctgg cgtaccgttc ctgtctaaaa tccctttaat
     7861 cggcctcctg tttagctccc gctctgattc taacgaggaa agcacgttat acgtgctcgt
     7921 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7981 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8041 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     8101 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     8161 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     8221 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     8281 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     8341 tttaacaaaa atttaacgcg aa
//
