LOCUS       sequence-490775-        8142 bp    DNA     circular SYN 27-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_093, derived from human fusion protein KMT2A_MLLT10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8142)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 27, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_093
FEATURES             Location/Qualifiers
     source          1..8142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1241..1260)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1306..1361
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1360..1379)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1722..1738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1722..1738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1735..1757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1746..1762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1770..1776,1777..1794,1795..1800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1815..1836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1895..2090
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1941..2076
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2096..2230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2133..2152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2187..2206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2298..2315)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2469..3057)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2549..2568)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3228..4019,4020..4088))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3851..3870
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4089..4193)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4224..4603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             4605..4862
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4909..5034
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5526..5643
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6003..6236
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6421..6465
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        6722..7025
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7026..7229
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7180..7200
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      7305..7314
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7311..7313,7314..7316,7317..8027)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7356..7377)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7617..7636)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7964..7985
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaaaaaaaaa atacaaagag aaagacaagc acaaacagaa gcataaaaag cagcccgagc
       61 cttccccggc gctggttcca tccctgacgg ttactacaga aaaaacgtac accagcactt
      121 ccaataactc aattagcggt tcccttaagc gcttggagga caccacggct cgttttacca
      181 atgctaactt tcaggaagtg tccgcccaca ccagttccgg caaagatgtt agcgagacac
      241 gcggctctga aggtaaaggt aaaaagtcct cggcgcattc ttctggtcag cgtggccgca
      301 agccgggcgg tggccgtaat ccgggtacaa cggtgtccgc ggcctcaccg ttcccgcagg
      361 gtagcttctc cggcaccccg ggttctgtga agagcagctc cggcagcagt gtccagagtc
      421 cacaagattt cctgagcttc accgactcag acttgcgtaa tgacagctat tctcacagcc
      481 agcagtcctc cgcgaccaag gacgtgcata agggtgagag tggttcacaa gagggtggcg
      541 tgaactcttt ctctaccttg attggtctgc catccacctc agcggtgacc agtcaaccga
      601 aatctttcga aaactccccg ggcgatctgg gaaacagctc cctcccgact gcgggttata
      661 aacgcgccca aacctccggc attgaagagg aaaccgtgaa agagaagaag cgtaagggta
      721 acaaacagag taagcacggg ccgggtcgcc caaagggcaa caagaaccaa gaaaacgtga
      781 gccacctgtc tgtgtcatca gcgagcccga ccagctcggt ggccagcgct gcgggtagta
      841 taacctcatc ctctctgcag aagtccccga cccttctccg taacggttca ctccagagcc
      901 tctctgttgg ttcctccccg gtaggaagcg aaatcagtat gcaatatcgt cacgatggtg
      961 cgtgcccgac cacgacattc tccgaactgt gaaccggttc tagataagat tacggtacct
     1021 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     1081 ggactggaag ggctaattca ctcccaaaga agacaagatc tgctttttgc ctgtactggg
     1141 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1201 cttaagcctc aataaagctt cagctgctcg agctagcaga tctttttccc tctgccaaaa
     1261 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     1321 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     1381 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     1441 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     1501 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     1561 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     1621 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     1681 ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     1741 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1801 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1861 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
     1921 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     1981 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2041 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     2101 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2161 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2221 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2281 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2341 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaagcagg ggataacgca
     2401 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2461 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2521 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2581 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2641 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2701 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2761 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2821 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2881 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2941 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3001 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3061 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3121 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3181 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3241 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3301 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3361 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3421 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3481 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3541 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3601 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3661 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3721 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     3781 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3841 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3901 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3961 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4021 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4081 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4141 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4201 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat
     4261 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     4321 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     4381 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     4441 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     4501 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     4561 tacttggcag tacatctacg tattagtcat cgctattacc atgggaggcg tggcctgggc
     4621 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     4681 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4741 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4801 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4861 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     4921 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     4981 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     5041 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     5101 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     5161 actggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     5221 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     5281 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     5341 caaaacaaaa gtaagaaaaa agcacagcaa gcagcaggat cttcagacct ggaaattccc
     5401 tacaatcccc aaagtcaagg agtagtagaa tctatgaata aagaattaaa gaaaattata
     5461 ggacaggtaa gagatcaggc tgaacatctt aagacagcag tacaaatggc agtattcatc
     5521 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     5581 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     5641 tttcgggttt attacaggga cagcagaaat ccactttgga aaggaccagc aaagctcctc
     5701 tggaaaggtg aaggggcagt agtaatacaa gataatagtg acataaaagt agtgccaaga
     5761 agaaaagcaa agatcattag ggattatgga aaacagatgg caggtgatga ttgtgtggca
     5821 agtagacagg atgaggatta gaacatggaa aagtttagta aaacaccata aggaggagat
     5881 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     5941 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     6001 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     6061 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     6121 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     6181 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     6241 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     6301 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     6361 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     6421 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     6481 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     6541 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     6601 ttatcgtttc agacccacct cccaaccccg aggggaccga gctcaagctt cgaacgcgtg
     6661 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     6721 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     6781 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     6841 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     6901 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     6961 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     7021 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     7081 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     7141 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     7201 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgggctcgt ttagtgaacc
     7261 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca
     7321 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     7381 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     7441 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     7501 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     7561 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     7621 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     7681 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     7741 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     7801 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     7861 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     7921 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     7981 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc ggaagcggag
     8041 gctccggggg aagtgcggcc gcttcccggg gcagcaaccg cagctacgac cagtccctga
     8101 gcgatagcag ttctcactct caggataaac accatgaaaa ag
//
