LOCUS       sequence-490783-        7377 bp    DNA     circular SYN 27-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_139, derived from human fusion protein PAX5_FOXP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7377)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 27, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_139
FEATURES             Location/Qualifiers
     source          1..7377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(910..1620,1621..1623,1624..1626))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(952..973)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1301..1320
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1560..1581
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1623..1632
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1708..1911)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1737..1757)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1912..2215
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2472..2516)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2701..2934
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3294..3411
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3903..4028
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4075..4332
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4334..4713
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4744..4848
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4849..4917,4918..5709)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5067..5086)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5880..6468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6369..6388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6622..6639
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6707..6841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6731..6750)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6785..6804
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(6847..7042)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6861..6996
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7101..7122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7137..7142,7143..7160,7161..7167)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7175..7191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7180..7202
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7199..7215
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7199..7215
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca ctccatgtct tcgttaacgg gttcgtcttc
      601 ataatcgcga tcatgatcga aatccgggga atggttcgct gtagtcacca gcgacagggg
      661 gccctcggct tcctccggat ctaaaggctc ctcctttacg tgaactgggt gaaccgcctg
      721 cattgggcta cgacctgggg aggagtcaga ttcattggaa tttgtgtgct ccattgctcc
      781 gtttaattct tcgcggattg cgctagctaa attaccaagg gttgggtttc ccatagatgc
      841 agtagtatat aacggaattg aattttcccg ggaagcggcc gcacttcccc cggagcctcc
      901 gcttccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      961 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg actgggtgct
     1021 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
     1081 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt
     1141 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
     1201 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
     1261 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
     1321 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
     1381 gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt
     1441 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
     1501 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
     1561 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
     1621 caccatggtg gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca gaattcggtt
     1681 cactaaacga gcccggttca ctaaacgagc tctgcttata tagacctccc accgtacacg
     1741 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
     1801 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
     1861 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
     1921 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg
     1981 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat
     2041 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg
     2101 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg
     2161 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa
     2221 ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagcacg
     2281 cgttcgaagc ttgagctcgg tcccctcggg gttgggaggt gggtctgaaa cgataatggt
     2341 gaatatccct gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct
     2401 accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta
     2461 aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc attcttttct
     2521 tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct
     2581 ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa
     2641 ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc
     2701 aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg
     2761 atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg
     2821 ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac
     2881 gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc
     2941 actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat
     3001 ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct
     3061 cctccttatg gtgttttact aaacttttcc atgttctaat cctcatcctg tctacttgcc
     3121 acacaatcat cacctgccat ctgttttcca taatccctaa tgatctttgc ttttcttctt
     3181 ggcactactt ttatgtcact attatcttgt attactactg ccccttcacc tttccagagg
     3241 agctttgctg gtcctttcca aagtggattt ctgctgtccc tgtaataaac ccgaaaattt
     3301 tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct
     3361 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg
     3421 aatactgcca tttgtactgc tgtcttaaga tgttcagcct gatctcttac ctgtcctata
     3481 attttcttta attctttatt catagattct actactcctt gactttgggg attgtaggga
     3541 atttccaggt ctgaagatcc tgctgcttgc tgtgcttttt tcttactttt gttttgctct
     3601 tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa
     3661 tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat
     3721 ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag gccagtatta
     3781 actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt atattttttc
     3841 tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata
     3901 ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta
     3961 ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt
     4021 cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag
     4081 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
     4141 gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt
     4201 tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac
     4261 aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc agtcccgccc
     4321 aggccacgcc tcccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     4381 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     4441 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     4501 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     4561 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     4621 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     4681 attactatta ataactagtc aataatcaat gtcgacccag gtggcacttt tcggggaaat
     4741 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4801 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4861 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4921 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4981 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5041 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5101 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5161 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5221 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5281 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5341 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5401 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5461 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5521 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5581 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5641 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5701 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5761 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5821 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5881 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6001 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6541 ttatcccctg cttctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6601 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6661 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcggatc cagacatgat
     6721 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     6781 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     6841 tagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     6901 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     6961 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     7021 ggttgctgac taattgagat gcggatccgc tggcacgaca ggtttcccga ctggaaagcg
     7081 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7141 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7201 ggaaacagct atgaccatga ttacgccaag cttgggctgc aggtcgaggg atctccataa
     7261 gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc tggctagtaa
     7321 aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac aaaatat
//
