LOCUS       sequence-490786-        8544 bp    DNA     circular SYN 27-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_182, derived from human fusion protein SS18_SSX1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8544)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 27, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_182
FEATURES             Location/Qualifiers
     source          1..8544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(2077..2787,2788..2790,2791..2793))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2119..2140)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2468..2487
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2727..2748
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2790..2799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2875..3078)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2904..2924)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3079..3382
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3639..3683)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3868..4101
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4461..4578
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5070..5195
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5242..5499
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        5501..5880
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5911..6015
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6016..6084,6085..6876)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6234..6253)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7047..7635
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7536..7555
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7789..7806
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7874..8008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7898..7917)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7952..7971
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(8014..8209)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8028..8163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    8268..8289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8304..8309,8310..8327,8328..8334)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8342..8358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8347..8369
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8366..8382
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8366..8382
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca ttcatcatct tcctctggat cagaaatctc
      601 ttcatagatt actaattgtt tacgttcacg aaggcgatgt gtccacgcat gcttgccacg
      661 ttttgggcct gaacgcttat taattttttc gctaatattt gctttaccag gaggatgtaa
      721 ctgtttacca tcattctgtg ggccactagc ctctgagact cctttagagt cgttttcgtc
      781 ttctgccggc ttttttggca tgatctggtc gtacccgtat gggcgctgct gcggtggttg
      841 aggtggccca ggttgcgtgg gacggtatcc accatactgc tggccttgtc cttgaggata
      901 gttggggtat tgcggtcctg gtccaccttg tgacggtccg tagccctgct gttgacctgg
      961 gtagccttgt tgacccggat attgctgttg ctggggagga tatccctgtt gaggcggagg
     1021 cccttggtag gcgtcttgct gctgcccata ttgggagttt cctccctcat agtagtgctg
     1081 ggaactatct tcatagggac gatcgtagcc ctgttcgggg tacgaaggtt gctggtagcc
     1141 atagtcgttg tgaccgtctg gataatactg ttggttcatc ccttccggcg gcccctgacc
     1201 cccgtgacta tattgatctc catagtagtc ctcttggcct gaatactgtt gtggcggtcc
     1261 ctgttgggga gggcgataag gagggatctg gcgttggccc atcatgtgat tgccctggtt
     1321 cacttgcccc atcatcccca tgggaggctg ctgaccttga taatgttgtc caccaccctg
     1381 tggcatgttg tactgttggg agggaggttg ctgatgcatc atcggaccct gatttggttg
     1441 catggacata ttcgggcgtg gcccgtaatt acccataggc tggccctgcg acattgtcat
     1501 ttggttttga accggcatag actgtgagga tggtaccgag tggttgtagc cacccatgga
     1561 gccatgcgag ctggaaggca tattcatact cgaattagtc atgttaagtt ggttaggacc
     1621 aggtccctgc atgggcatgt ggttggggcc gggcatctga ccgttcatct ggttctgcat
     1681 gtgcggtgct gggggtccgc caccgaccat cccatcggat ggcatgttat gtgagcgagg
     1741 gggaggtggt ggacccgact gattcatgcc cccagggccc atgggcatat tttgcgtagg
     1801 tggcgctggc aacaacgatt gcatgttctg gttgctgtct gcgatagtag ccaagtaaac
     1861 aagattcgta tgcaacatct gttgatattg agaacattcc gaggttttgc ccttgttttg
     1921 actatccatg atgcattgga ttaagtggtt attgtcatca agcatctttt ggatcgcagc
     1981 gggcgtaatc tcgccttttc cacgctgacg cggggccgcg aacgcgactg acatccggga
     2041 agcggccgca cttcccccgg agcctccgct tccgcccttg tacagctcgt ccatgccgag
     2101 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
     2161 gtctttgctc agcttggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
     2221 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
     2281 gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata
     2341 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
     2401 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
     2461 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
     2521 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa
     2581 gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
     2641 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
     2701 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
     2761 cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtggat cccgggcccg
     2821 cggtaccgtc gactgcagaa ttcggttcac taaacgagcc cggttcacta aacgagctct
     2881 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     2941 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     3001 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     3061 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     3121 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     3181 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     3241 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     3301 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     3361 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     3421 aattgattac tattaataac tagcacgcgt tcgaagcttg agctcggtcc cctcggggtt
     3481 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
     3541 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
     3601 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
     3661 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
     3721 attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa
     3781 atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg
     3841 agttttccag agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca
     3901 gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt
     3961 tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt
     4021 ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag
     4081 aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc
     4141 tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat
     4201 tcacttctcc aattgtccct catatctcct ccttatggtg ttttactaaa cttttccatg
     4261 ttctaatcct catcctgtct acttgccaca caatcatcac ctgccatctg ttttccataa
     4321 tccctaatga tctttgcttt tcttcttggc actactttta tgtcactatt atcttgtatt
     4381 actactgccc cttcaccttt ccagaggagc tttgctggtc ctttccaaag tggatttctg
     4441 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
     4501 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
     4561 cccccttttc ttttaaaatt gtggatgaat actgccattt gtactgctgt cttaagatgt
     4621 tcagcctgat ctcttacctg tcctataatt ttctttaatt ctttattcat agattctact
     4681 actccttgac tttggggatt gtagggaatt tccaggtctg aagatcctgc tgcttgctgt
     4741 gcttttttct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct
     4801 tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta
     4861 agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct
     4921 tctgatgttt ctaacaggcc agtattaact gcgaatcgtt ctagctccct gcttgcccat
     4981 actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat
     5041 cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag
     5101 cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg
     5161 cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc
     5221 aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     5281 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     5341 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     5401 tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat gcaggatctg
     5461 agggctcgcc actccccagt cccgcccagg ccacgcctcc catggtaata gcgatgacta
     5521 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     5581 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     5641 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     5701 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     5761 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     5821 ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc
     5881 gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5941 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6001 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6061 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6121 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6181 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6241 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6301 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6361 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6421 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6481 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6541 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6601 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6661 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6721 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6781 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6841 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6901 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6961 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7021 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7081 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7141 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7201 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7261 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7321 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7381 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7441 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7501 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7561 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7621 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7681 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7741 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7801 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7861 cattaatgca gcggatccag acatgataag atacattgat gagtttggac aaaccacaac
     7921 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     7981 aaccattata agctgcaata aacaagttag ctttttgcaa aagcctaggc ctccaaaaaa
     8041 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     8101 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     8161 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgcg gatccgctgg
     8221 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     8281 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     8341 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
     8401 gggctgcagg tcgagggatc tccataagag aagagggaca gctatgactg ggagtagtca
     8461 ggagaggagg aaaaatctgg ctagtaaaac atgtaaggaa aattttaggg atgttaaaga
     8521 aaaaaataac acaaaacaaa atat
//
