LOCUS       sequence-490794-       10545 bp    DNA     circular SYN 27-OCT-2025
DEFINITION  Express mEGFP-tagged fusion protein NUP98_NSD1 with an IDR deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10545)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 27, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_NUP98_NSD1-Delta-1-746
FEATURES             Location/Qualifiers
     source          1..10545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4078..4788,4789..4791,4792..4794))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4120..4141)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4469..4488
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4728..4749
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4791..4800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4876..5079)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4905..4925)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5080..5383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5640..5684)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5869..6102
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6462..6579
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7071..7196
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7243..7500
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7502..7881
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7912..8016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8017..8085,8086..8877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8235..8254)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9048..9636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9537..9556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9790..9807
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    9875..10009
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9899..9918)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9953..9972
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(10015..10210)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10029..10164
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    10269..10290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10305..10310,10311..10328,10329..10335)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10343..10359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10348..10370
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10367..10383
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10367..10383
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca ctacttctgt tctgattctg cacacttgtg
      601 actggaagga gcctggttaa gagctggaag tgtattttgc tctggtttgg ggtcttgccc
      661 tcttccaaga gaccagcaag tctgtgccaa tgtctgtgtg ctcccagcag accagcaaga
      721 ctgtgccagt gtctgtccag ccactatggg ccagagccct gcccgtgatg aggcttttcc
      781 cagggcccag ggactttgct ctgtggttac caacttcttt tcttcagtgg ggagggaggc
      841 tggggccttc ccgagagacc taaaagattg cccactcttg gcggcaagtg caggtagttg
      901 ctggcctccg accatctgct tcgcctgctt caccaggccc ggggattggc taagaggccc
      961 tggggtctgc tcagcccctg cagaagctct tcctgaggcc tgagttgttg gctcatataa
     1021 aaaggccttg gcaggaggct gagaaagaag tctggcctgg gtgagtgatt taacagcttg
     1081 ccaggggtcc tctgaagggg ctgctgcttt cccagatgtc tgaagaggat ctgacacaca
     1141 gcctttctca acagctctcg gcatatgccc cagacctttg ctggcaggcc atgaacttga
     1201 ctccaacact ggcatcttct catcagcctg tggtgtgacc tgatgaggtg aagctgcccg
     1261 ctccttctgg cgaggagtta ggtctatgag atccatcacc aaagcagctc tattttttga
     1321 ctgagtattc tggtccacag gcctcagtgc tttttcttta gctacaaggg tctggaccac
     1381 agctgatagt actttctcag gaggtgggag tctctgggac aaagaggcat ggggtttgtc
     1441 agtaggcctg tctgaagtct ggggtttggg actgctagta atgagcagtc tgtctggcgg
     1501 cggaagtctc aggccagtgg gaactgaggt tttctccagt gactggggcc ttgggctggc
     1561 agcacctatg gatttatcca gccttgggtc agccgtcccc agaggtcttt ccagtggttg
     1621 ggacctgact gagggtgagg agcttgggct ggtcactgga gagggtttgt cgcttagctg
     1681 gggtcttggt cctgccactg ctggtggcct gtccagtggc ctctggctgg aaaccaagga
     1741 ttgggatttg gtccctgacc cagcgaggtc tctgacctta tctaaaggct ggggcctgga
     1801 gtcagttctc tcaagaggtc tttcaggtag caatggcctc tgacaagtcc ctgccagagc
     1861 ttttttggag agcgacagca tctggttggt gtcagcagga ggtttatctg acattttggg
     1921 tgcctgagca gccattcctg ttgattgctc tgccaggtga gtgcttggcc ctggaggcag
     1981 cggtactggg ggaggcacat actcacggat ctccccaggt tccagaggat tgggcccaca
     2041 ggggtcatgc tcagtacaag acagacgccc atccagtttg gaaatgaaaa gcatcccttc
     2101 tcgatgctgc ttacaaaagg agctggggca catctcacag aaggaggctg cttccttccc
     2161 gcagatgtca cactgatgcc acggacattc ccatttccct gctggtcgct tggtcagatt
     2221 gagacagtct gcgtggtaaa cttttgggca gcctggtttc ttgcaggaga cgagctggcc
     2281 agcatcccca caactaaaac actcatcttc tcgctccttt gtgatttcac cctgggtcct
     2341 gcgctttccc tgttgcttct tcttgaattt ctttgacttt tcttccgtgg caatgggttg
     2401 attctttggc cttacaccca agaagccact gcagttcggg gctccacatt tgcaaacagt
     2461 ctttccattc ccaagacatt ctaggttgta gttgaaggta agttcagtgc ctgctttaat
     2521 gtcacttagt gcaaaaaggc ctacacgggt atctccattc acagaccact tctgtgtttc
     2581 acagttgggc tggcagcaat gattcatgaa ccgagcatag tttcctttgg gaccagcatc
     2641 aatgattcgg tctttgtcta gggtgagcat atagaaatta gtgatatcat gttcttgagc
     2701 atagcgaatt cgagctctgc attcttcttc atctataagc tcacccacat actcattcac
     2761 aaattcaccc tttttaatat ctgtttttgt ccgtagaccc caaccccgct gtaatgtgcg
     2821 gaaaatttca acctctggat attggcgctt ggaaaagcac tggttttgac agcgccctcc
     2881 ggcaggacac actgtggggt ggcactcata gagcagcatg cggttgatgc attcagagtc
     2941 tatcccacag gggttctcat cagtagcttt acagttgcaa cggggtattt cagataagtc
     3001 tgcagtgaag atctgtaccc tgccaatagg acggtttacc tttatatgtt tataaggtgg
     3061 tggcttcttg tcattctttc ggtcttcctg cagctgtctt agctcttttt gggcctttaa
     3121 ttcctcaaac cttgctgcag cttcctgaag agctttttta tatgtcccat ccactccttt
     3181 gcccatctta tccttgctgc tcacgtcacc ctccatgtaa gggaagactc gggcctggtg
     3241 agtccacaaa tagtcattag atccaaaaaa gaggactggg aactctccca catcatgtct
     3301 catcttatca atgttggaag gaacagctcg aggatggcag atctcagctg gccaccacct
     3361 gtatcgtcca acttttaccc agacaatctc cctgtagtgt ggctttttgc ctgctttaca
     3421 gtcattgcaa taccagtttc cttcagggat atcaatgttc aggcattcac gatgaaaagc
     3481 agcagggcaa gaatcacagc acagaaggct gcctccttct gagcacacaa agcaccagct
     3541 aacattaaca tgctcatgat ttcggcagcc ccgcctaggg gtaaagtgat tagggcagat
     3601 gatactatta gatgcaagga tctttgaccc agcagccagg caaaagtcat tggcgtggta
     3661 tgccacagga cagcggacac agcgcatcaa ccgaccttta gatgcagaaa cattggctgg
     3721 attagcagca tgacaggtta tacagatgtg gagggagcac cggaagccct tgttctgcat
     3781 aacagtgggt gggtacttct ggacacactc ttcatggtaa aactttccac acaagggtag
     3841 aaggcacctt ttaacatctt ccccactctg cttacataca aaacaggtat ggattcctgt
     3901 gcgacattca ttgcagataa attttcctct tggcatctca gtcaatccaa ggcactccag
     3961 gtggaaagcc ccacagcact gagcctcaca taacagcagc tcacccaatt tttcacaatt
     4021 ctgacagaca ttctcccggg aagcggccgc acttcccccg gagcctccgc ttccgccctt
     4081 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
     4141 gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tgggtgctca ggtagtggtt
     4201 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
     4261 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
     4321 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
     4381 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
     4441 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
     4501 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
     4561 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
     4621 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
     4681 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
     4741 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc
     4801 gaccggtgga tcccgggccc gcggtaccgt cgactgcaga attcggttca ctaaacgagc
     4861 ccggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     4921 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     4981 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     5041 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     5101 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     5161 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     5221 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     5281 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     5341 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     5401 gctatgaact aatgaccccg taattgatta ctattaataa ctagcacgcg ttcgaagctt
     5461 gagctcggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc
     5521 ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct
     5581 actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac
     5641 aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc
     5701 gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc
     5761 atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa
     5821 ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga
     5881 tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact
     5941 gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc
     6001 actataccag acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata
     6061 gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt
     6121 tctctctgca ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt
     6181 actactttat atttatataa ttcacttctc caattgtccc tcatatctcc tccttatggt
     6241 gttttactaa acttttccat gttctaatcc tcatcctgtc tacttgccac acaatcatca
     6301 cctgccatct gttttccata atccctaatg atctttgctt ttcttcttgg cactactttt
     6361 atgtcactat tatcttgtat tactactgcc ccttcacctt tccagaggag ctttgctggt
     6421 cctttccaaa gtggatttct gctgtccctg taataaaccc gaaaattttg aatttttgta
     6481 atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc
     6541 cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt
     6601 tgtactgctg tcttaagatg ttcagcctga tctcttacct gtcctataat tttctttaat
     6661 tctttattca tagattctac tactccttga ctttggggat tgtagggaat ttccaggtct
     6721 gaagatcctg ctgcttgctg tgcttttttc ttacttttgt tttgctcttc ctctatcttg
     6781 tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata gagggttgct
     6841 actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg ttgtagctgt
     6901 cccagtattt gtctacagcc ttctgatgtt tctaacaggc cagtattaac tgcgaatcgt
     6961 tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc
     7021 cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg
     7081 cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc
     7141 cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc
     7201 tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca
     7261 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
     7321 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
     7381 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag gcaaaaagca
     7441 gctgcttata tgcaggatct gagggctcgc cactccccag tcccgcccag gccacgcctc
     7501 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     7561 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     7621 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     7681 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     7741 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     7801 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     7861 aactagtcaa taatcaatgt cgacccaggt ggcacttttc ggggaaatgt gcgcggaacc
     7921 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7981 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8041 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8101 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8161 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8221 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8281 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8341 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8401 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8461 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8521 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     8581 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8641 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8701 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8761 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8821 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8881 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8941 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9001 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9061 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9121 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9181 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9241 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9301 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9361 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9421 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9481 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9541 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9601 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9661 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     9721 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     9781 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     9841 ctccccgcgc gttggccgat tcattaatgc agcggatcca gacatgataa gatacattga
     9901 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     9961 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta gctttttgca
    10021 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
    10081 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
    10141 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
    10201 attgagatgc ggatccgctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    10261 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    10321 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    10381 gaccatgatt acgccaagct tgggctgcag gtcgagggat ctccataaga gaagagggac
    10441 agctatgact gggagtagtc aggagaggag gaaaaatctg gctagtaaaa catgtaagga
    10501 aaattttagg gatgttaaag aaaaaaataa cacaaaacaa aatat
//
