LOCUS       sequence-490860-        8605 bp    DNA     circular SYN 28-OCT-2025
DEFINITION  Rat bipolar mutant CAPS1 R994L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8605)
  TITLE     Martin lab plasmids
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CAPS1 R944L mKate2
FEATURES             Location/Qualifiers
     source          1..8605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     614..630
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     615..631
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      4567..4576
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4573..5271
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    5393..5514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5430..5449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5484..5503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5521..5976)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5658..5679)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5870..5889
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6003..6107
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6109..6466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6109..6300
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6132..6152)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6317..6452
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6501..7295
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6501..7295
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6555..6574)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7165..7184
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7483..7502)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7527..7574
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7903..8491
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8392..8411
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggt cgacggtatc gccaccatgc tggacccttc gtccagcgaa
      661 gaggaatcgg atgagatcct ggaagaggag agcggcaagg aggtgctggg ctcggccgcg
      721 tccggagcgc gcctgtctcc aagccgcacc aacgagggct cggcgggcag cgccggcatg
      781 gggggcagtg gcgccggggc cggggtgggc gccggcggcg gtgggggtag cggcgcgagc
      841 agcggcggcg gggccggggg gctgcagccc agcagccgcg ccggcggcgg ccggccctcc
      901 agccccagcc cgtcggtggt gagcgagaag gagaaggaag agttggagcg gctgcagaag
      961 gaagaggagg agaggaagaa gaggctgcag ctgtacgtgt tcgtgatgcg ctgcattgcc
     1021 taccccttca acgccaagca gcccaccgac atggctcggc ggcagcagaa gatcagcaaa
     1081 cagcagctgc agactgtcaa ggaccgattc caggctttcc tcaatgggga gacccaaatc
     1141 gtggcagatg aagcctttat gaatgctgtc cagagctact atgaggtgtt tctcaagagt
     1201 gaccgtgtgg cccgcatggt acagagtggg ggctgctcag ccaacgactc tcgggaggtc
     1261 ttcaagaaac acatcgagaa gagggtacgc agcctacctg agattgatgg cctcagcaag
     1321 gagactgtgc taagctcctg gatggctaag tttgacgcca tctaccgtgg tgaggaggac
     1381 cccaggaagc agcaagcccg gatgacagcc agtgcagctt ctgagttgat tctgagcaag
     1441 gaacagctct atgagatgtt ccagaacatt cttgggatca agaaatttga acatcaactc
     1501 ctatataatg cgtgtcagct ggacaatcca gatgagcagg cagcccagat cagacgagag
     1561 ctggacggac gtctccaaat ggcagaccaa atagccaggg aacgcaaatt tcctaagttc
     1621 gtatccaaag aaatggaaaa tatgtacatc gaggagctga agtcctccgt caacctactc
     1681 atggccaact tggagagcat gccggtgtcc aagggtggag agttcaagct gcagaaactg
     1741 aaacgcagtc acaacgcgtc catcattgac atgggtgagg agagcgagaa ccagctgtcc
     1801 aagtcggatg tcctgctgtc cttctccttg gaggttgtca tcatggaagt ccaaggcctt
     1861 aagtccttgg ctccaaaccg aatcgtgtac tgcacaatgg aggtggaggg tggagagaaa
     1921 cttcagactg atcaagcaga ggcttctaaa ccaacctggg gcactcaagg cgacttctct
     1981 acaacccacg cgctgccagc tgttaaagta aagctattca cagagagcac tggtgtcctg
     2041 gccttggaag acaaggaact cgggcgggtt atcctccatc ccaccccaaa cagccccaaa
     2101 caatccgagt ggcacaaaat gacagtctcc aaaaactgcc ccgatcaaga cctcaaaatc
     2161 aaactcgctg tccgaatgga taagcctcaa aacatgaagc actctgggta cttatggacc
     2221 attggtaaga atgtgtggaa gaggtggaag aaaaggttct ttgtgttggt gcaggtcagc
     2281 cagtacactt ttgccatgtg cagctatcga gaaaagaaag cagaacctca ggaactccta
     2341 cagctggatg gctacaccgt ggattacacc gaccctcagc caggtttgga gggtggccga
     2401 gccttcttca atgcagtcaa agaaggagac acggtgatat ttgccagtga cgatgagcaa
     2461 gatcgaatcc tgtgggtcca agctatgtac agggccaccg ggcagtcgca caaacctgtg
     2521 ccccctaccc aggtgcagaa gctcaacgcc aagggcggaa acgtgcctca gctggatgcc
     2581 cccatctccc agttttacgc agatagagct caaaagcatg gcatggatga atttatctcg
     2641 tccaacccct gtaactttga ccacgcttcc ctctttgaga tggtacagcg gctgactttg
     2701 gatcacagac taaacgattc ctattcttgc ctgggctggt tcagtcctgg ccaggtgttt
     2761 gtgttagacg agtactgcgc tcggaatgga gtccgaggat gtcatcggca tctctgttac
     2821 cttagagact tgctggaacg ggcagaaaat ggcgccatga tcgaccccac gcttcttcat
     2881 tacagctttg ccttctgcgc atcccatgtc cacgggaaca ggcctgatgg aatcggaact
     2941 gtgactgttg aagaaaaaga gcgctttgaa gaaatcaaag agagactccg agttctgctg
     3001 gaaaatcaaa ttacacattt caggtactgc tttccgtttg gtcgacctga aggtgctttg
     3061 aaagcgactc tctcactttt ggaacgggtt ttgatgaaag atattgtcac tccagtaccc
     3121 caagaggagg tcaaaactgt catccgtaaa tgcctagagc aggctgctct agtcaactac
     3181 tctcgcctat ctgagtatgc caaaatcgaa gaaaatgtag gccgcttaat cactcccgcc
     3241 aaaaagctcg aagacacaat ccgtcttgct gaactagtca ttgaagtgct gcaacaaaac
     3301 gaagagcacc acgccgaggc ctttgcgtgg tggtcagact taatggtgga gcacgcggag
     3361 acgttcctgt cactctttgc agtggacatg gatgcagcat tagaggtgca acccccagac
     3421 acgtgggaca gtttcccact cttccagctg ctgaatgact ttctccttac agactataac
     3481 ttgtgcaatg gaaagtttca caaacacctg caggatctgt tcgccccact cgttgttaga
     3541 tacgtggatc tgatggagtc ctcaattgca caatctatcc acaggggctt tgagcgggag
     3601 tcatgggaac cagtcaataa cggatcgggt acatcagaag atttgttctg gaaacttgat
     3661 gcccttcaga ctttcatccg tgacctacac tggcctgagg aagagtttgg aaagcactta
     3721 gagcagcggc taaaactaat ggcaagcgac atgattgagt cctgcgtcaa gagaaccagg
     3781 attgcatttg aagttaagct gcaaaaaacc agtcgatcaa cagattttcg agtcccacag
     3841 tcaatatgca ccatgtttaa tgttatggtt gatgccaaag ctcaatcaac aaaactttgc
     3901 agcatggaaa tgggccagga gcatcaatac cattcaaaaa tagacgaact aattgaagaa
     3961 accgttaagg aaatgatcac actcttggtg gcaaagtttg taactatatt ggaaggcgta
     4021 ctggcaaagt tatccagata cgacgagggg actttgtttt cttcgtttct gtcatttacg
     4081 gtgaaggcgg catctaagta tgtggacgta cctaaacccg ggatggatgt ggccgacgcc
     4141 tacgtgactt tcgtccgcca ctctcaggat gtccttcgtg ataaggtcaa tgaggagatg
     4201 tatatagaaa ggttatttga tcaatggtac aacagctcca tgaacgtcat ctgcacctgg
     4261 ctgaccgacc ggatggacct ccagcttcac atttatcagc tgaaaacact aattaggatg
     4321 gtaaagaaaa cctacagaga tttccgattg caaggggtcc tggattccac cttaaacagc
     4381 aagacctatg agaccattcg aaatcggctc actgtagagg aagctaccgc gtccgtgagt
     4441 gagggagggg gcttgcaagg catcagcatg aaggacagcg atgaagagga cgaggaggat
     4501 gacgaattcc accacactgg actagtggat ccgagctcgg taccgcgggc ccgggatcca
     4561 ccggtcgcca ccatggtgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg
     4621 gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac
     4681 gagggcaccc agaccatgag aatcaaggcg gtcgagggcg gccctctccc cttcgccttc
     4741 gacatcctgg ctaccagctt catgtacggc agcaaaacct tcatcaacca cacccagggc
     4801 atccccgact tctttaagca gtccttcccc gagggcttca catgggagag agtcaccaca
     4861 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
     4921 atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt gatgcagaag
     4981 aaaacactcg gctgggaggc ctccaccgag accctgtacc ccgctgacgg cggcctggaa
     5041 ggcagagccg acatggccct gaagctcgtg ggcgggggcc acctgatctg caacttgaag
     5101 accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactatgtg
     5161 gacagaagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag
     5221 gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacagatg agcggccgcg
     5281 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     5341 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     5401 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5461 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt
     5521 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     5581 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     5641 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     5701 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     5761 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     5821 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     5881 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     5941 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     6001 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6061 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     6121 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6421 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     6481 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     6541 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6601 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6661 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     6721 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6781 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6841 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6901 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6961 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     7021 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     7081 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     7141 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     7201 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     7261 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     7321 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     7381 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     7441 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     7501 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     7561 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     7621 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     7681 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     7741 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     7801 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7861 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7921 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7981 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8041 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8101 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8161 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8221 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8281 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8341 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8401 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8461 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8521 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     8581 ggataaccgt attaccgcca tgcat
//
