LOCUS       sequence-491003-        5234 bp    DNA     circular SYN 29-OCT-2025
DEFINITION  Mammalian expression of N-terminal Myc-tagged rat Rab3A wild type.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5234)
  TITLE     Martin lab plasmids 2025 (Yamaga)
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 29, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEF-cMyc-Rab3A
FEATURES             Location/Qualifiers
     source          1..5234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     837..857
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      913..922
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             922..951
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1789..1910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1826..1845)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1880..1899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1917..2372)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2054..2075)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2266..2285
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2399..2503
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2528..2548)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2572..2782
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2594..2790
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2641..2776
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2825..3619
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2825..3619
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2879..2898)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3489..3508
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3807..3826)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3851..3898
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4227..4815
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4716..4735
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(4945..5234,1..889)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(5176..5234,1..880)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
ORIGIN
        1 gccttgaatt acttccacct ggcagcagta cgtgattctt gatcccgagc ttcgggttgg
       61 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
      121 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
      181 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
      241 ttttttctgg caagatagtc ttgtaaatgc gggccaagat cagcacactg gtatttcggt
      301 ttttgggggc gcgggcggcg acgggggccg tgcgtcccag cgcacatgtt cggcgaggcg
      361 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
      421 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
      481 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg gagggtgctc
      541 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
      601 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
      661 gcacctcgat tagttgtcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
      721 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
      781 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
      841 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattggcta
      901 gcgctaccgg tcgccaccat ggaacaaaaa cttatttcag aagaagattt gtccggactc
      961 agatctcgag ctcaagcttc gaattctgca gtcgacatgg cctcagccac agactctcga
     1021 tatgggcaga aggagtcctc agaccagaac ttcgactata tgttcaagat cctgatcatt
     1081 ggtaacagca gtgtgggcaa aacctcattc cttttccgct acgcagacga ctcttttact
     1141 ccagcctttg tcagcactgt gggcatagac ttcaaggtca aaaccatcta ccgcaatgac
     1201 aagaggatca agctgcagat ctgggacaca gcaggacaag agcggtaccg aaccatcacc
     1261 acagcctact accggggcgc catgggcttc attctaatgt atgacatcac caatgaggag
     1321 tccttcaatg cagtgcagga ctggtccact cagatcaaaa cttactcatg ggacaatgcc
     1381 caggtgctgc tggtggggaa caagtgcgac atggaggacg agcgagtggt gtcctcagaa
     1441 cgaggccggc agctggccga ccacctgggc tttgagttct ttgaggccag cgccaaggac
     1501 aacattaatg tcaagcagac ctttgaacgt ctggtggacg tgatctgtga gaagatgtcg
     1561 gagtccctag atactgcaga ccctgcagtc acgggtgcca agcagggccc acagctcacc
     1621 gaccagcagg caccacctca tcaggattgc gcctgctgag gatccaccgg atctagataa
     1681 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     1741 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     1801 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1861 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat
     1921 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     1981 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2041 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2101 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2161 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     2221 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     2281 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     2341 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     2401 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2461 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     2521 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     2581 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     2641 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     2701 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     2761 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     2821 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     2881 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     2941 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     3001 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     3061 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     3121 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     3181 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     3241 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     3301 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     3361 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     3421 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     3481 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     3541 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     3601 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     3661 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     3721 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     3781 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     3841 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     3901 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     3961 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     4021 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     4081 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4141 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4201 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4261 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4321 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4381 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4441 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4501 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4561 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4621 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4681 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4741 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4801 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4861 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4921 gccatgcatg cggccgcctc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4981 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     5041 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     5101 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     5161 gccgccagaa cacaggtaag tgccgtgtgt ggttccggcg ggcctggcct ctttacgggt
     5221 tatggccctt gcgt
//
