LOCUS       sequence-491019-        6297 bp    DNA     circular SYN 29-OCT-2025
DEFINITION  HSPA1A signal monomer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Zheng L, Yan Y, Zhou B, Lim J, Shi D, An B, Ko B, Klyder E, 
            Pitchiaya S, Cai DJ, Boyden ES, Wei D, Lio P, Linghu C
  TITLE     Multiplexed, scalable analog recording of gene regulation dynamics 
            over weeks using intracellular protein tapes
  JOURNAL   bioRxiv 2025.05.10.653182
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 29, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.05.10.653182"
COMMENT     Sequence Label: HSPA1A-1POK(E239Y,L349K)-dMBP-V5
FEATURES             Location/Qualifiers
     source          1..6297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   72..201
                     /label=AAV2 ITR
     CDS             3283..3324
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    3349..3556
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3726..3855
                     /label=AAV2 ITR
     rep_origin      complement(4092..4680)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4172..4191)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4851..5642,5643..5711))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5474..5493
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5712..5816)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5842..6297)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5979..6000)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6191..6210
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
      121 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      181 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt
      241 agccatgctc taggaagatc gtaggttaat taaactaggg aactttccag tactttccca
      301 aacaaggacc tactgagcct ttcaggttca caatcaatca gatccctact ggctcaccta
      361 gtctcccgac gccttcgctt cagtttggaa acgtccagat tacgcagccc cagcgagtag
      421 gtgggggctc cctcaatatc aaactgcaca accggggtcc ccccaccccc caccccgtcc
      481 ctccctgcaa atttgagacg gctccaactc agtaatcttt ttccaaactg gcccatgagg
      541 tcagagacag tatctccatt gtaacgtggc cgggcggtgt caacacaaac gcccccaccc
      601 tcccctggac gcgcgtaacc cgctccccgc accagccccc tgcccacaac tgcgcaggcc
      661 cagcaagccc ccacaattaa aagcccagcg ccgacccttc ctgtcaatta ggcgctgaag
      721 cgcaggcggt cagcatcgcc atggagacca acacccttcc caccgccact cccccttcct
      781 ctcagggtcc ctgtcccctc cagtgaatcc cagaagactc tggagagttc tgagcagggg
      841 gcggcactct ggcctctgat tggtccaagg aaggctgggg ggcaggacgg gaggcgaaaa
      901 ccctggaata ttcccgacct ggcagcctca tcgagctcgg tgattggctc agaagggaaa
      961 aggcgggtct ccgtgacgac ttataaaagc ccaggggcaa gcggtccgga tacgactagt
     1021 gccaccatga ttgactatac tgctgctggt ttcaccctcc tccaaggtgc acatctgtac
     1081 gctcctgaag acagaggtat ctgcgatgtg ctggttgcca acggtaagat catcgctgtg
     1141 gcctccaaca ttcctagcga catcgttcca aactgcaccg ttgtcgatct gagcggacag
     1201 atcctctgtc caggattcat tgatcagcac gtgcacctca ttggaggagg aggcgaagct
     1261 ggtcctacta ctagaactcc cgaagtcgca ctgtctcgcc tcacagaggc aggcgttacc
     1321 tccgtcgtgg gactcctggg tactgatagc atctctaggc accctgagtc tctgctggca
     1381 aagaccagag ccctcaatga ggagggtatc tccgcttgga tgctgactgg tgcttatcac
     1441 gtcccatctc gcaccattac tggcagcgtg gagaaggacg ttgcaatcat cgacagagtg
     1501 atcggtgtta agtgcgctat ctccgaccac aggagcgctg ctcccgacgt ctatcacctg
     1561 gccaacatgg cagccgagag cagagtcggt ggtctgctcg gtggcaagcc aggagttacc
     1621 gtgttccaca tgggtgactc taagaaggca ctgcaaccca tctatgacct gctggagaac
     1681 tgtgacgtcc caatctccaa gctgctgcct acccacgtca acaggaacgt gcctctcttc
     1741 taccaagctc tggagttcgc aagaaagggt ggcaccatcg acatcacctc tagcattgat
     1801 gaacccgtcg ctccagctga gggaatcgca agggctgtcc aagcaggtat cccactggca
     1861 agggtgacac tcagctctga tggcaacggc tcccagccat tcttcgatga tgaaggaaat
     1921 ctgactcaca ttggcgtggc tggatttgaa actctcctgg aaacagttca ggttctggtt
     1981 aaggactacg acttcagcat ttctgacgct ctcagaccac tcacctctag cgtcgctggc
     2041 ttcctgaacc tgactggtaa aggcgaaatc aaacctggta atgacgctga tctgctggtg
     2101 atgacacccg aactgcgcat tgagcaggtc tatgcaagag gaaagctgat ggttaaggac
     2161 ggaaaggctt gtgtcaaggg cacattcgag actgctggca aactggttat ctggatcaat
     2221 ggcgataagg gctacaatgg cctggcagag gtcggtaaga agttcgagaa agatacaggc
     2281 atcaaagtta ctgtggaaca cccagataag ctggaggaga agttccctca ggtcgcagca
     2341 actggcgacg gtccagacat catcttctgg gcacacgata ggttcggtgg ctatgcccaa
     2401 tctggcctgc tggctgagat tactcctgat aaggctttcc aggacaagct gtatccattc
     2461 acctgggatg ctgtgagata caatggcaag ctgatcgcat accctattgc tgtggaagct
     2521 ctgagcctga tctacaacaa ggacctgctg cctaatcctc ctaagacatg ggaagaaatc
     2581 cctgcactgg acaaggaact gaaggccaaa ggcaagtccg cactgatgtt caacctgcag
     2641 gagccttact ttacctggcc actgattgct gccgacggtg gttacgcttt caagtacgag
     2701 aatggtaagt acgacatcaa agatgtgggt gtggacaatg ctggtgccaa agctggtctg
     2761 actttcctgg tggatctgat caagaacaag cacatgaatg cagacactga ctattctatc
     2821 gcagaggctg ctttcaacaa aggcgaaacc gcaatgacta tcaatggtcc ttgggcatgg
     2881 tctaacatcg acactagcaa agtcaactac ggtgtcaccg ttctgccaac cttcaagggt
     2941 cagccaagca aacctttcgt tggcgtgctg agcgcaggta tcaacgctgc ctctcctaac
     3001 aaagagctgg ctaaggagtt tctggagaac tacctgctga cagacgaagg tctggaggca
     3061 gtgaacaagg acaagccact gggtgccgtg gctctgaaga gctacgaaga agaactggcc
     3121 aaggaccctc gcatcgctgc tacaatggag aacgcacaga agggtgagat catgccaaac
     3181 atcccacaga tgagcgcatt ctggtatgcc gtgaggaccg ctgttatcaa cgcagcttct
     3241 ggcagacaga ccgtggatga agccctgaaa gacgcacaga ccggtaagcc tatccctaac
     3301 cctctgctcg gtctcgattc tacctaagcg gccgctcata aagctcgact gtgccttcta
     3361 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     3421 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     3481 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     3541 gcaggcatgc tggggatgcg gtccggactg tacaattgca ttcattttat gtttcaggtt
     3601 cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     3661 gataaggatc ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa
     3721 ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     3781 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     3841 cgagcgagcg cgcagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3901 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3961 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4021 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4081 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4141 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4201 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4261 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4321 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4381 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4441 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4501 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4561 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4621 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4681 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4741 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4801 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4861 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4921 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4981 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5041 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5101 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5161 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5221 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5281 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5341 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5401 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5461 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5521 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5581 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5641 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5701 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5761 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5821 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     5881 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     5941 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     6001 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     6061 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     6121 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     6181 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     6241 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgt
//
