LOCUS       sequence-491021-        6786 bp    DNA     circular SYN 29-OCT-2025
DEFINITION  Egr1 signal monomer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Zheng L, Yan Y, Zhou B, Lim J, Shi D, An B, Ko B, Klyder E, 
            Pitchiaya S, Cai DJ, Boyden ES, Wei D, Lio P, Linghu C
  TITLE     Multiplexed, scalable analog recording of gene regulation dynamics 
            over weeks using intracellular protein tapes
  JOURNAL   bioRxiv 2025.05.10.653182
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 29, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.05.10.653182"
COMMENT     Sequence Label: Egr1-1POK(E239Y,L349K)-dMBP-V5
FEATURES             Location/Qualifiers
     source          1..6786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   72..201
                     /label=AAV2 ITR
     protein_bind    complement(280..300)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1257..1277
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1304..1322
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1386..1404)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(1428..1452)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3772..3813
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    3838..4045
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4215..4344
                     /label=AAV2 ITR
     rep_origin      complement(4581..5169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4661..4680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5340..6131,6132..6200))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5963..5982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6201..6305)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6331..6786)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6468..6489)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6680..6699
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
      121 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      181 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt
      241 agccatgctc taggaagatc gtaggttaat taaacgcgtc aacttttcta tacaaagttg
      301 agacaccgtg ccatagatcg aggcccgggg tcaaggcccc gcctctcctg ggcggcccct
      361 gcccaggcgg gcccagccgc tcctcccccg cactcccggt tcgctctcac ggtccctgag
      421 gtgggcgggc gggccctgga tgacagcgat agaaccccgg cccgactcgc cctcgccccc
      481 gctctgggtc tgggcttccc cagcctagtt cacgcctagg agccgcctga gcagccgcgc
      541 gcccagcgcc acacgccacg agccctcccc gcctgggcgt ccccggatcc cgcgagcgct
      601 cgggctcccg gcttggaacc agggaggagg gagggagcga gggagcaacc agctgcgacc
      661 cggaaatgcc atataaggag caggaaggat cccccgccgg aacaaccctt atttgggcag
      721 caccttattt ggagtggccc gatatggccc ggccgcttcc ggctctggga ggagggaaga
      781 aggcggaggg aggggcaacg cgggaactcc ggagctgcgc gggtcccgga ggccccggcg
      841 gcggctagag ctctaggctt ccccgaagcc tgggcgcctg ggatgcgggc gcgggcgcgg
      901 gccctagggt gcaggatgga ggtgccgggc gctgtcggat ggggggcttc acgtcgctcc
      961 gggtcctccc ggccggtcct gccatattag ggcttcctgc ttcccatata tggccatgta
     1021 cgtcacgacg gaggcggacc cgtgccgttc cagacccttc aaatagaggc ggatccgggg
     1081 agtcgcgaga gatcccagcg cgcagaactt ggggagccgc cgccgccatc cgccgccgca
     1141 gccagcttcc gccgccgcag gaccggcccc tgccccagcc tccgcagccg cggcgcgtcc
     1201 acgcccgccc gcgcccaggg cgagtcgggg tcgccgcctg cacgcttctc agtgttcaac
     1261 tttgtataat aaagttgagc tagttgctaa gtctgccgag tagaattaac cctcactaaa
     1321 gggtaggtga cactatatca tctacaaact ttacatagac cgacaacatt ctatacttct
     1381 atactcctat agtgagtcgt attagtagac gcgagtcaat gcatgtacga cagctttctt
     1441 gtacaaagtg gttcgatatc aagatctggc ctcggcggcc aagcttggca atccggtact
     1501 gttggtaaag ccaccatgat tgactatact gctgctggtt tcaccctcct ccaaggtgca
     1561 catctgtacg ctcctgaaga cagaggtatc tgcgatgtgc tggttgccaa cggtaagatc
     1621 atcgctgtgg cctccaacat tcctagcgac atcgttccaa actgcaccgt tgtcgatctg
     1681 agcggacaga tcctctgtcc aggattcatt gatcagcacg tgcacctcat tggaggagga
     1741 ggcgaagctg gtcctactac tagaactccc gaagtcgcac tgtctcgcct cacagaggca
     1801 ggcgttacct ccgtcgtggg actcctgggt actgatagca tctctaggca ccctgagtct
     1861 ctgctggcaa agaccagagc cctcaatgag gagggtatct ccgcttggat gctgactggt
     1921 gcttatcacg tcccatctcg caccattact ggcagcgtgg agaaggacgt tgcaatcatc
     1981 gacagagtga tcggtgttaa gtgcgctatc tccgaccaca ggagcgctgc tcccgacgtc
     2041 tatcacctgg ccaacatggc agccgagagc agagtcggtg gtctgctcgg tggcaagcca
     2101 ggagttaccg tgttccacat gggtgactct aagaaggcac tgcaacccat ctatgacctg
     2161 ctggagaact gtgacgtccc aatctccaag ctgctgccta cccacgtcaa caggaacgtg
     2221 cctctcttct accaagctct ggagttcgca agaaagggtg gcaccatcga catcacctct
     2281 agcattgatg aacccgtcgc tccagctgag ggaatcgcaa gggctgtcca agcaggtatc
     2341 ccactggcaa gggtgacact cagctctgat ggcaacggct cccagccatt cttcgatgat
     2401 gaaggaaatc tgactcacat tggcgtggct ggatttgaaa ctctcctgga aacagttcag
     2461 gttctggtta aggactacga cttcagcatt tctgacgctc tcagaccact cacctctagc
     2521 gtcgctggct tcctgaacct gactggtaaa ggcgaaatca aacctggtaa tgacgctgat
     2581 ctgctggtga tgacacccga actgcgcatt gagcaggtct atgcaagagg aaagctgatg
     2641 gttaaggacg gaaaggcttg tgtcaagggc acattcgaga ctgctggcaa actggttatc
     2701 tggatcaatg gcgataaggg ctacaatggc ctggcagagg tcggtaagaa gttcgagaaa
     2761 gatacaggca tcaaagttac tgtggaacac ccagataagc tggaggagaa gttccctcag
     2821 gtcgcagcaa ctggcgacgg tccagacatc atcttctggg cacacgatag gttcggtggc
     2881 tatgcccaat ctggcctgct ggctgagatt actcctgata aggctttcca ggacaagctg
     2941 tatccattca cctgggatgc tgtgagatac aatggcaagc tgatcgcata ccctattgct
     3001 gtggaagctc tgagcctgat ctacaacaag gacctgctgc ctaatcctcc taagacatgg
     3061 gaagaaatcc ctgcactgga caaggaactg aaggccaaag gcaagtccgc actgatgttc
     3121 aacctgcagg agccttactt tacctggcca ctgattgctg ccgacggtgg ttacgctttc
     3181 aagtacgaga atggtaagta cgacatcaaa gatgtgggtg tggacaatgc tggtgccaaa
     3241 gctggtctga ctttcctggt ggatctgatc aagaacaagc acatgaatgc agacactgac
     3301 tattctatcg cagaggctgc tttcaacaaa ggcgaaaccg caatgactat caatggtcct
     3361 tgggcatggt ctaacatcga cactagcaaa gtcaactacg gtgtcaccgt tctgccaacc
     3421 ttcaagggtc agccaagcaa acctttcgtt ggcgtgctga gcgcaggtat caacgctgcc
     3481 tctcctaaca aagagctggc taaggagttt ctggagaact acctgctgac agacgaaggt
     3541 ctggaggcag tgaacaagga caagccactg ggtgccgtgg ctctgaagag ctacgaagaa
     3601 gaactggcca aggaccctcg catcgctgct acaatggaga acgcacagaa gggtgagatc
     3661 atgccaaaca tcccacagat gagcgcattc tggtatgccg tgaggaccgc tgttatcaac
     3721 gcagcttctg gcagacagac cgtggatgaa gccctgaaag acgcacagac cggtaagcct
     3781 atccctaacc ctctgctcgg tctcgattct acctaagcgg ccgctcataa agctcgactg
     3841 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3901 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3961 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     4021 aagacaatag caggcatgct ggggatgcgg tccggactgt acaattgcat tcattttatg
     4081 tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     4141 ggtaaaatcg ataaggatct tcctagagca tggctacgta gataagtagc atggcgggtt
     4201 aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     4261 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     4321 ctcagtgagc gagcgagcgc gcagctgcat taatgaatcg gccaacgcgc ggggagaggc
     4381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     4441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     4501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4741 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4981 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     5041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     5101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     5281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     5401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     5461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     5521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     5581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     5641 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     5701 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     5761 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     5821 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     5881 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     5941 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     6001 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     6061 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     6121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     6181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     6241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     6301 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     6361 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     6421 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     6481 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     6541 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     6601 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     6661 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     6721 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     6781 gcgcgt
//
