LOCUS       sequence-491026-        7224 bp    DNA     circular SYN 29-OCT-2025
DEFINITION  c-fos signal monomer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Zheng L, Yan Y, Zhou B, Lim J, Shi D, An B, Ko B, Klyder E, 
            Pitchiaya S, Cai DJ, Boyden ES, Wei D, Lio P, Linghu C
  TITLE     Multiplexed, scalable analog recording of gene regulation dynamics 
            over weeks using intracellular protein tapes
  JOURNAL   bioRxiv 2025.05.10.653182
REFERENCE   2  (bases 1 to 7224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 29, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.05.10.653182"
COMMENT     Sequence Label: c-fos-1POK(E239Y,L349K)-dMBP-V5
FEATURES             Location/Qualifiers
     source          1..7224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     CDS             complement(904..915)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4139..4180
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    4205..4412
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4582..4711
                     /label=AAV2 ITR
     rep_origin      complement(4948..5536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5028..5047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5707..6498,6499..6567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6330..6349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6568..6672)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6698..7153)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6835..6856)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7047..7066
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg taggttaatt aacaagcttt cctttaggaa cagaggcttc gagcctttaa
      241 ggctgcgtac ttgcttctcc taataccaga gactcaaaaa aaaaaaaaaa gttccagatt
      301 gctggacaat gacccgggtc tcatcccttg accctgggaa ccgggtccac attgaatcag
      361 gtgcgaatgt tcgctcgcct tctctgcctt tcccgcctcc cctcccccgg ccgcggcccc
      421 ggttcccccc ctgcgctgca ccctcagagt tggctgcagc cggcgagctg ttcccgtcaa
      481 tccctccctc ctttacacag gatgtccata ttaggacatc tgcgtcagca ggtttccacg
      541 gccggtccct gttgttctgg gggggggacc atctccgaaa tcctacacgc ggaaggtcta
      601 ggagaccccc taagatccca aatgtgaaca ctcataggtg aaagatgtat gccaagacgg
      661 gggttgaaag cctggggcgt agagttgacg acagagcgcc cgcagagggc cttggggcgc
      721 gcttcccccc ccttccagtt ccgcccagtg acgtaggaag tccatccatt cacagcgctt
      781 ctataaaggc gccagctgag gcgcctacta ctccaaccgc gactgcagcg agcaactgag
      841 aagactggat agagccggcg gttccgcgaa cgagcagtga ccgcgctccc acccagctct
      901 gctctgcagc tcccaccagt gtctacccct ggaccccttg ccgggctttc cccaaacttc
      961 gaccttctcg ggtttcaacg ccgactacga ggcgtcatcc tcccgctgca gtagcgcctc
     1021 cccggccggg gacagccttt cctactacca ttccccagcc gactccttct ccagcggctc
     1081 tcctgtcaac acacaggtga gtttggcttt gtgtagccgc caggtccgcg ctgagggtcg
     1141 ccgtggagga gacactgggg tgtgactcgc aggggcgggg gggtcttcct ttttcgctct
     1201 ggagggagac tggcgcggtc agagcagcct tagcctggga acccaggact tgtctgagcg
     1261 cgtgcacact tgtcatagta agacttagtg accccttccc gcgcggcagg tttattctga
     1321 gtggcctgcc tgcattcttc tctcggccga cttgtttctg agatcagccg gggccaacaa
     1381 gtctcgagca aagagtcgct aactagagtt tgggaggcgg caaaccgcgg caatcccccc
     1441 tcccggggca gcctggagca gggaggaggg aggagggagg agggtgctgc gggcgggtgt
     1501 gtaaggcagt ttcattgata aaaagcgagt tcattctgga gactccggag cagcgcctgc
     1561 gtcagcgcag acgtcaggga tatttataac aaaccccctt tcgagcgagt gatgccgaag
     1621 ggataacggg aacgcagcag taggatggag gagaaaggct gcgctgcgga attcaaggga
     1681 ggatattggg agagctttta tctccgatga ggtgcataca ggaagacata agcagtctct
     1741 gaccggaatg cttctctctc cctgcttcat gcgacactag ggccacttgc tccacctgtg
     1801 tctggaacct cctcgctcac ctccgctttc ctctttttgt tttgtttcag gacttttgcg
     1861 cagatcgata actagtgcca ccatgattga ctatactgct gctggtttca ccctcctcca
     1921 aggtgcacat ctgtacgctc ctgaagacag aggtatctgc gatgtgctgg ttgccaacgg
     1981 taagatcatc gctgtggcct ccaacattcc tagcgacatc gttccaaact gcaccgttgt
     2041 cgatctgagc ggacagatcc tctgtccagg attcattgat cagcacgtgc acctcattgg
     2101 aggaggaggc gaagctggtc ctactactag aactcccgaa gtcgcactgt ctcgcctcac
     2161 agaggcaggc gttacctccg tcgtgggact cctgggtact gatagcatct ctaggcaccc
     2221 tgagtctctg ctggcaaaga ccagagccct caatgaggag ggtatctccg cttggatgct
     2281 gactggtgct tatcacgtcc catctcgcac cattactggc agcgtggaga aggacgttgc
     2341 aatcatcgac agagtgatcg gtgttaagtg cgctatctcc gaccacagga gcgctgctcc
     2401 cgacgtctat cacctggcca acatggcagc cgagagcaga gtcggtggtc tgctcggtgg
     2461 caagccagga gttaccgtgt tccacatggg tgactctaag aaggcactgc aacccatcta
     2521 tgacctgctg gagaactgtg acgtcccaat ctccaagctg ctgcctaccc acgtcaacag
     2581 gaacgtgcct ctcttctacc aagctctgga gttcgcaaga aagggtggca ccatcgacat
     2641 cacctctagc attgatgaac ccgtcgctcc agctgaggga atcgcaaggg ctgtccaagc
     2701 aggtatccca ctggcaaggg tgacactcag ctctgatggc aacggctccc agccattctt
     2761 cgatgatgaa ggaaatctga ctcacattgg cgtggctgga tttgaaactc tcctggaaac
     2821 agttcaggtt ctggttaagg actacgactt cagcatttct gacgctctca gaccactcac
     2881 ctctagcgtc gctggcttcc tgaacctgac tggtaaaggc gaaatcaaac ctggtaatga
     2941 cgctgatctg ctggtgatga cacccgaact gcgcattgag caggtctatg caagaggaaa
     3001 gctgatggtt aaggacggaa aggcttgtgt caagggcaca ttcgagactg ctggcaaact
     3061 ggttatctgg atcaatggcg ataagggcta caatggcctg gcagaggtcg gtaagaagtt
     3121 cgagaaagat acaggcatca aagttactgt ggaacaccca gataagctgg aggagaagtt
     3181 ccctcaggtc gcagcaactg gcgacggtcc agacatcatc ttctgggcac acgataggtt
     3241 cggtggctat gcccaatctg gcctgctggc tgagattact cctgataagg ctttccagga
     3301 caagctgtat ccattcacct gggatgctgt gagatacaat ggcaagctga tcgcataccc
     3361 tattgctgtg gaagctctga gcctgatcta caacaaggac ctgctgccta atcctcctaa
     3421 gacatgggaa gaaatccctg cactggacaa ggaactgaag gccaaaggca agtccgcact
     3481 gatgttcaac ctgcaggagc cttactttac ctggccactg attgctgccg acggtggtta
     3541 cgctttcaag tacgagaatg gtaagtacga catcaaagat gtgggtgtgg acaatgctgg
     3601 tgccaaagct ggtctgactt tcctggtgga tctgatcaag aacaagcaca tgaatgcaga
     3661 cactgactat tctatcgcag aggctgcttt caacaaaggc gaaaccgcaa tgactatcaa
     3721 tggtccttgg gcatggtcta acatcgacac tagcaaagtc aactacggtg tcaccgttct
     3781 gccaaccttc aagggtcagc caagcaaacc tttcgttggc gtgctgagcg caggtatcaa
     3841 cgctgcctct cctaacaaag agctggctaa ggagtttctg gagaactacc tgctgacaga
     3901 cgaaggtctg gaggcagtga acaaggacaa gccactgggt gccgtggctc tgaagagcta
     3961 cgaagaagaa ctggccaagg accctcgcat cgctgctaca atggagaacg cacagaaggg
     4021 tgagatcatg ccaaacatcc cacagatgag cgcattctgg tatgccgtga ggaccgctgt
     4081 tatcaacgca gcttctggca gacagaccgt ggatgaagcc ctgaaagacg cacagaccgg
     4141 taagcctatc cctaaccctc tgctcggtct cgattctacc taagcggccg ctcataaagc
     4201 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4261 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4321 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4381 gattgggaag acaatagcag gcatgctggg gatgcggtcc ggactgtaca attgcattca
     4441 ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta
     4501 caaatgtggt aaaatcgata aggatcttcc tagagcatgg ctacgtagat aagtagcatg
     4561 gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc
     4621 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     4681 gggcggcctc agtgagcgag cgagcgcgca gctgcattaa tgaatcggcc aacgcgcggg
     4741 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4801 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4861 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4921 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4981 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5041 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5101 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5161 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5221 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5281 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5341 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5401 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5461 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5521 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5581 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5641 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5701 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5761 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5821 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     5881 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     5941 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6001 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6061 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6121 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6181 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6241 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6301 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6361 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6421 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6481 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6541 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6601 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6661 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     6721 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     6781 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     6841 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     6901 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     6961 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     7021 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     7081 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     7141 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     7201 cgggcctctt cgctattacg ccag
//
