LOCUS       sequence-491028-        5942 bp    DNA     circular SYN 29-OCT-2025
DEFINITION  Structure monomer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Zheng L, Yan Y, Zhou B, Lim J, Shi D, An B, Ko B, Klyder E, 
            Pitchiaya S, Cai DJ, Boyden ES, Wei D, Lio P, Linghu C
  TITLE     Multiplexed, scalable analog recording of gene regulation dynamics 
            over weeks using intracellular protein tapes
  JOURNAL   bioRxiv 2025.05.10.653182
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 29, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.05.10.653182"
COMMENT     Sequence Label: UbC-1POK(E239Y,L349K)-dMBP-HA
FEATURES             Location/Qualifiers
     source          1..5942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   72..201
                     /label=AAV2 ITR
     promoter        274..672
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             2943..2969
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2994..3201
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3371..3500
                     /label=AAV2 ITR
     rep_origin      complement(3737..4325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3817..3836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4496..5287,5288..5356))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5119..5138
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5357..5461)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5487..5942)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5624..5645)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5836..5855
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
      121 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      181 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt
      241 agccatgctc taggaagatc gtaggttaat taaggcctcc gcgccgggtt ttggcgcctc
      301 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc
      361 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
      421 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
      481 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
      541 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
      601 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
      661 atcgtcactt ggcgactagt gccaccatga ttgactatac tgctgctggt ttcaccctcc
      721 tccaaggtgc acatctgtac gctcctgaag acagaggtat ctgcgatgtg ctggttgcca
      781 acggtaagat catcgctgtg gcctccaaca ttcctagcga catcgttcca aactgcaccg
      841 ttgtcgatct gagcggacag atcctctgtc caggattcat tgatcagcac gtgcacctca
      901 ttggaggagg aggcgaagct ggtcctacta ctagaactcc cgaagtcgca ctgtctcgcc
      961 tcacagaggc aggcgttacc tccgtcgtgg gactcctggg tactgatagc atctctaggc
     1021 accctgagtc tctgctggca aagaccagag ccctcaatga ggagggtatc tccgcttgga
     1081 tgctgactgg tgcttatcac gtcccatctc gcaccattac tggcagcgtg gagaaggacg
     1141 ttgcaatcat cgacagagtg atcggtgtta agtgcgctat ctccgaccac aggagcgctg
     1201 ctcccgacgt ctatcacctg gccaacatgg cagccgagag cagagtcggt ggtctgctcg
     1261 gtggcaagcc aggagttacc gtgttccaca tgggtgactc taagaaggca ctgcaaccca
     1321 tctatgacct gctggagaac tgtgacgtcc caatctccaa gctgctgcct acccacgtca
     1381 acaggaacgt gcctctcttc taccaagctc tggagttcgc aagaaagggt ggcaccatcg
     1441 acatcacctc tagcattgat gaacccgtcg ctccagctga gggaatcgca agggctgtcc
     1501 aagcaggtat cccactggca agggtgacac tcagctctga tggcaacggc tcccagccat
     1561 tcttcgatga tgaaggaaat ctgactcaca ttggcgtggc tggatttgaa actctcctgg
     1621 aaacagttca ggttctggtt aaggactacg acttcagcat ttctgacgct ctcagaccac
     1681 tcacctctag cgtcgctggc ttcctgaacc tgactggtaa aggcgaaatc aaacctggta
     1741 atgacgctga tctgctggtg atgacacccg aactgcgcat tgagcaggtc tatgcaagag
     1801 gaaagctgat ggttaaggac ggaaaggctt gtgtcaaggg cacattcgag actgctggca
     1861 aactggttat ctggatcaat ggcgataagg gctacaatgg cctggcagag gtcggtaaga
     1921 agttcgagaa agatacaggc atcaaagtta ctgtggaaca cccagataag ctggaggaga
     1981 agttccctca ggtcgcagca actggcgacg gtccagacat catcttctgg gcacacgata
     2041 ggttcggtgg ctatgcccaa tctggcctgc tggctgagat tactcctgat aaggctttcc
     2101 aggacaagct gtatccattc acctgggatg ctgtgagata caatggcaag ctgatcgcat
     2161 accctattgc tgtggaagct ctgagcctga tctacaacaa ggacctgctg cctaatcctc
     2221 ctaagacatg ggaagaaatc cctgcactgg acaaggaact gaaggccaaa ggcaagtccg
     2281 cactgatgtt caacctgcag gagccttact ttacctggcc actgattgct gccgacggtg
     2341 gttacgcttt caagtacgag aatggtaagt acgacatcaa agatgtgggt gtggacaatg
     2401 ctggtgccaa agctggtctg actttcctgg tggatctgat caagaacaag cacatgaatg
     2461 cagacactga ctattctatc gcagaggctg ctttcaacaa aggcgaaacc gcaatgacta
     2521 tcaatggtcc ttgggcatgg tctaacatcg acactagcaa agtcaactac ggtgtcaccg
     2581 ttctgccaac cttcaagggt cagccaagca aacctttcgt tggcgtgctg agcgcaggta
     2641 tcaacgctgc ctctcctaac aaagagctgg ctaaggagtt tctggagaac tacctgctga
     2701 cagacgaagg tctggaggca gtgaacaagg acaagccact gggtgccgtg gctctgaaga
     2761 gctacgaaga agaactggcc aaggaccctc gcatcgctgc tacaatggag aacgcacaga
     2821 agggtgagat catgccaaac atcccacaga tgagcgcatt ctggtatgcc gtgaggaccg
     2881 ctgttatcaa cgcagcttct ggcagacaga ccgtggatga agccctgaaa gacgcacaga
     2941 cctacccata tgacgtccct gactatgcat aagcggccgc tcataaagct cgactgtgcc
     3001 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3061 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     3121 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     3181 caatagcagg catgctgggg atgcggtccg gactgtacaa ttgcattcat tttatgtttc
     3241 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     3301 aaatcgataa ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc
     3361 attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     3421 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     3481 gtgagcgagc gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3541 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3601 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3661 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3721 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3781 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3841 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3901 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3961 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4021 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4081 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4141 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4201 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4261 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4321 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4381 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4441 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4501 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4561 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4621 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4681 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4741 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4801 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4861 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4921 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4981 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5041 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5101 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5161 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5221 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5281 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5341 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5401 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5461 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     5521 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     5581 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     5641 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     5701 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     5761 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     5821 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     5881 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     5941 gt
//
