LOCUS       sequence-491038-       10346 bp    DNA     circular SYN 29-OCT-2025
DEFINITION  active kinase domain of Rok fused to the light sensitive 
            dimerization domain CIBN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Bao M, Lepeta K, Aguilar G, Schnider S, Affolter M, Vigano MA
  TITLE     In vivo regulation of an endogenously tagged protein by a 
            light-regulated kinase.
  JOURNAL   G3 (Bethesda). 2025 Jun 4;15(6):jkaf073. doi: 
            10.1093/g3journal/jkaf073.
  PUBMED    40194518
REFERENCE   2  (bases 1 to 10346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 29, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2025-06-4"; volume: "15(6):jkaf073. doi"; 
            pages: " 10.1093/g3journal/jkaf073"
COMMENT     Sequence Label: pUASTattBRhokinase-CIBN
FEATURES             Location/Qualifiers
     source          1..10346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2313..2331
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     complement(2364..2383)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2365..2383)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2390..2407)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2390..2406)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2399..2421)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2548..3003
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2635..2654)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2845..2866
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3029..3133
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3134..3202,3203..3994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3352..3371)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4165..4753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4654..4673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4907..4924
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5041..5062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5077..5082,5083..5100,5101..5107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5115..5131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5120..5142
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5139..5155
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5139..5155
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5176..5194
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    5413..5482
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     polyA_signal    5511..5645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5535..5554)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5589..5608
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5917..5937)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6067..6132
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(6219..6245)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(6249..6758)
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     promoter        complement(8143..8381)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(8175..8196)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    8400..8494
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    complement(8527..8560)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(9335..9949,10020..10151,9..324,386..1040,
                     1115..1388,1790..1861))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 ccactcaccg ttgtctgaat aagtcgcact tttacgagga gtggttcctt gagcaccgac
       61 agccaggatc gccacaggac cgcccggaac tgcatgaacc aggtggcctt gtaggtgtac
      121 ccattctccg gctgctccag tggcttctcc agatttttgg tggccaacaa ctgctccata
      181 tcccgggcta ctttgctaat ggcaaaattg tcgcatatct tggcgatccg atcacgggac
      241 tcgatctccc gtccgggcac aacggccaac acctgtacgt aaaagtccgc cggattgtag
      301 ttggtaggac actgggcacc cacgctggat aggagttgag atgttatgta atactagata
      361 cccttaataa acacatcgaa ctcactagga aaagaagtcg acggcttcgc tgggagtgcc
      421 caagaaagct accctgccct cggccatcag aaggatcttg tcaaagagct caaacagctc
      481 ggaagacggc tgatgaatgg tcaggatgac ggtcttgccc ttctgcgaca gcttcttcag
      541 cacctggacg acgctgtggg cggtaaagga gtccagtccg gaggtgggct catcgcagat
      601 cagaagcggc ggatcggtta gagcctcgga ggcgaatgcc agacgcttcc tttctccgcc
      661 ggacagacct ttcaccctgc cgggcacacc gatgatcgtg tgctgacatt tgctgagcga
      721 aagctcctgg atcacctgat ccacgcgggc cactcgctgc cgataggtca gatgtcgtgg
      781 catccgcacc atggcttgga aaatcaggtg ttccctggcc gttagggagc cgataaagag
      841 gtcatcctgc tggacatagg cgcacctggc ctgcatctcc ttggcgtcca caggttggcc
      901 attgagcagt cgcatcccgg atggcgatac ttggatgccc tgcggcgatc gaaaggcaag
      961 ggcattcagc agggtcgtct ttccggcacc ggaactgccc atcacggcca aaagttcgcc
     1021 cggataggcc acgccgcaaa ctgagtttca aattggtaat tggacccttt attaagattt
     1081 cacacagatc agccgactgc gaatagaaac tcaccgttct tgagcaaatg tttcctgggc
     1141 gccggtatgt gtcgctcgtt gcagaatagt ccgcgtgtcc ggttgaccag ctgccgccat
     1201 ccggagcccg gctgattgac cgccccaaag atgtccatat tgtgccaggc ataggtgagg
     1261 ttctcggcta gttggccgct ccctgaaccg gagtcctccg gcggactggg tggccggagc
     1321 gtgccgtagt ttttggcctg cccgaagccc tggttaatgc agctctgcga agccgctccg
     1381 ctgtcaccct gcaatgatag gggatctcaa atatcaacta caagcgttat gctcatctaa
     1441 ccccgaacaa aaagtacccc gaagtatcct acgaagtagg tttatacttt tatttatttt
     1501 ttgtgcatct aggatcagct taaaatatct ggttgttata ttttttgtaa aaaagaatat
     1561 agtcgaaaat gaatgccttt agatgtcttg atcatgatat gatctcaaaa attgtcttat
     1621 atagcgagaa cagctaccag aataatctgt ttcgtgtcac tatttgtttg tgcaattgcg
     1681 gtttgggatt tttgtgggtc gcagttctca cgccgcagac aatttgatgt tgcaatcgca
     1741 gttcctatag atcaagtgaa cttaagatgt atgcacatgt actactcaca ttgttcagat
     1801 gctcggcaga tgggtgtttg ctgcctccgc gaattaatag ctcctgatcc tcttggccca
     1861 ttgccgggat ttttcacact ttcccctgct tacccaccca aaaccaatca ccaccccaat
     1921 cactcaaaaa acaaacaaaa ataagaagcg agaggagttt tggcacagca ctttgtgttt
     1981 aattgatggc gtaaaccgct tggagcttcg tcacgaaacc gctgacaaaa tgcaactgaa
     2041 ggcggacatt gacgctacgt aacgctacaa acggtggcga aagagatagc ggacgcagcg
     2101 gcgaaagaga cggcgatatt tctgtggaca gagaaggagg caaacagcgc tgactttgag
     2161 tggaatgtca ttttgagtga gaggtaatcg aaagaacctg gtacatcaaa tacccttgga
     2221 tcgaagtaaa tttaaaactg atcagataag ttcaatgata tccagtgcag taaaaaaaaa
     2281 aaatgttttt tttatctact ttccgcaaaa atgggtttta ttaacttaca tacatactag
     2341 gggcccggta cgtacccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
     2401 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     2461 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     2521 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     2581 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2641 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2701 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     2761 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2821 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2881 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2941 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     3001 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3061 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3121 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3181 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3241 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3301 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3361 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3421 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3481 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3541 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3661 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3721 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3781 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3841 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3961 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4021 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4081 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4741 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4801 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4861 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     4921 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     4981 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     5041 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     5101 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     5161 ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggcta gaactagtgt
     5221 cgacatgccc gccgtgaccg tcgagaaccc gctgacgctg ccccgcgtat ccgcacccgc
     5281 cgacgccgtc gcacgtcccg tgctcaccgt gaccaccgcg cccagcggtt tcgagggcga
     5341 gggcttcccg gtgcgccgcg cgttcgccgg gatcaactac cgccacctcg acccgttcat
     5401 catgatggac cagatgggtg aggtggagta cgcgcccggg gagcccaagg gcacgccctg
     5461 gcacccgcac cgcggcttcg agaccgtgac ctacatcgtc gacactagtg gatccagaca
     5521 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     5581 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     5641 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     5701 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ctctagtcaa
     5761 ggcactatac atcaaatatt tttccataat tttcttgtat agcagtgcag ctttttcctt
     5821 tgtggtgtaa atagcaaagc aagcaagagt tctattacta aacacagcat gactcaaaaa
     5881 acttagcaat tctgaaggaa agtccttggg gtcttctacc tttctcttct tttttggagg
     5941 agtagaatgt tgagagtcag cagtagcctc atcatcacta gatggcattt cttctgagca
     6001 aaacaggttt tcctcattaa aggcattcca ccactgctcc cattcatcag ttccataggt
     6061 tggaatctaa aatacacaaa caattagaat cagtagttta acacattata cacttaaaaa
     6121 ttttatattt accttagagc tttaaatctc tgtaggtagt ttgtccaatt atgtcacacc
     6181 acagaagtaa ggttccttca caaagatcct ctagattacg cgtagtctgg cacgtcgtag
     6241 gggtaatgaa tataatccgt tttctccaat tccttcgtca ctttagatga atcattagag
     6301 aaattgttct cttctttctt ggctttgtgt ttcatcttct tgatgctttt tgtgctccca
     6361 ttgttttgct ctgttatttt cgacttctct tcttctcctt cttctactag gtcatctctg
     6421 ttcatcgtca tcttcttcgc cgcctcatta caatccttag tctctgtatc aaacttcgct
     6481 gccttgaaat ttccagtccc aagcgtcgtt tccggcgaaa ttgagagtct tgaatcacct
     6541 tccaccgtcg tctcaccgta catcatcgga agattcaaac cggcagtcga aagatagctg
     6601 tccatctcgc cgccggtaat cattgaagaa tcggcaaaga agccggcgag aggagaatca
     6661 aaggtgggat tgaggtactt gaggtgagcc ctttggcgct ctaggaccga catgtcagga
     6721 aaattgagca aaaggtcacc tcctatagct ccattcatct cgagcgacgg ccgatggcgg
     6781 tgattatgac cgtggccgtg attattgctg gccgccccgc tgtttgccac attggcctcc
     6841 ttcgactccg catccacggt gtcgctagac agcagctgat agtcacccgt atacgtaaag
     6901 ccgatgaagg gcaagtgatt gccatcgaag cccttgggta cgggaaacac ttcctccggc
     6961 ttctcatcgc gctcaatgtc ctcgaaattt cgcgtatcat cgtcggagga gagttcgggc
     7021 accacaggcg gcacactctc gcgaatgttg tcgaacgacc aggtatcatt tcggaaaaac
     7081 ggatgcgcct taatgtcctc gatgccgtag cgacccaaac gttgcgttcg gtccgtgaga
     7141 aaggcgcgga tcagtgcctt cgcctgttcg ctgatctcaa cctcgggtgg aaagctaagc
     7201 gagttcttgt ggtccatgat cttgccgtag gtgcccacca gcgagtctgc gtagaatggt
     7261 gtctcgccga acagcatctc gtagaggaaa atgcccaccg accaccagtc gcattcccgc
     7321 ccgtactcgt tgtccacgcc ctgcgactgc agcacctccg ggctgatgta gtccggcgtg
     7381 ccaacggcat tgctggacac cacttggcca ttggcgccca ttcgcataca ggtgccaaag
     7441 tcggccaatt tcaggtggcc atagctgtcg aggagcatat tatccggctt aacgtcccga
     7501 tgcacgaagc ccatattgtg aatggtgtcc agcgcgagaa ccacctccat tgtgtaaaag
     7561 atggcccact tctccgggat gtcgtagtca cccatcagcg agactatatc gccgccgggc
     7621 ataaagtcca tcaccatgta taggtatttg gcatcctgaa atgcaaagtg cagctgaacg
     7681 atccattcgg agttggcgtg cgccataatg tgtcgctcct cccagaagaa cgcggagtcc
     7741 ggtcgcttca tcatctcgaa tttggacagc cgcttcatgg cgtacacctg gctggaggac
     7801 ttgtgccgca ccagctgcac ctctccaaag gcgccggcgc cgatgagttt gataaaatgg
     7861 aaatcctcga cgttcatgcg cagctgattg atctgcattg ccaatggttt gtatttggcc
     7921 gcatactgct cgatgttctt aagccgcctc agggactcgt ggtcacaatc gctgaccaag
     7981 gccgacaccg tgtccagcag gcagtccaca ttgcagatgc tggtcggatc gcgcatctcg
     8041 cgctcgagcg tattcgcccg gcgccttcgt tccacatcca tgctgcgctg cttggtcaca
     8101 gtttctcgtc cagctggcat tttgaattcc caattcccta ttcagagttc tcttcttgta
     8161 ttcaataatt acttcttggc agatttcagt agttgcagtt gatttacttg gttgctggtt
     8221 acttttaatt gattcacttt aacttgcact ttactgcaga ttgtttagct tgttcagctg
     8281 cgcttgttta tttgcttagc tttcgcttag cgacgtgttc actttgcttg tttgaattga
     8341 attgtcgctc cgtagacgaa gcgcctctat ttatactccg gcgctcgcta gagtctccgc
     8401 tcggaggaca gtactccgct cggaggacag tactccgctc ggaggacagt actccgctcg
     8461 gaggacagta ctccgctcgg aggacagtac tccgacctgc aggcatgcaa gcttggatcc
     8521 gctagcataa cttcgtatag catacattat acgaagttat gctagcctag ttccagtgaa
     8581 atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt tttgatatat
     8641 ataaacaaca ctattatgcc caccattttt ttgagatgca tctacacaag gaacaaacac
     8701 tggatgtcac tttcagttca aattgtaacg ctaatcactc cgaacaggtc acaaaaaatt
     8761 accttaaaaa gtcataatat taaattagaa taaatatagc tgtgagggaa atatatacaa
     8821 atatattgga gcaaataaat tgtacataca aatatttatt actaatttct attgagacga
     8881 aatgaaccac tcggaaccat ttgagcgaac cgaatcgcgc ggaactaacg acagtcgctc
     8941 caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac agcgaaagag
     9001 caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga tttgaaaagt
     9061 atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt ttgatgtaag
     9121 gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact tgtggggatg
     9181 ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg cacaaatata
     9241 attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata atggtaaaaa
     9301 aaaaaaacaa gcagttattt cggatatatg tcggctactc cttgcgtcgg gcccgaagtc
     9361 ttagagccag atatgcgagc acccggaagc tcacgatgag aatggccaga cccacgtagt
     9421 ccagcggcag atcggcggcg gagaagttaa gcgtctccag gatgaccttg cccgaactgg
     9481 ggcacgtggt gttcgacgat gtgcagctaa tttcgcccgg ctccacgtcc gcccattggt
     9541 taatcagcag accctcgttg gcgtaacgga accatgagag gtacgacaac catttgaggt
     9601 atactggcac cgagcccgag ttcaagaaga agccgccaaa gagcaggaat ggtatgataa
     9661 ccggcggacc cacagacagc gccatcgagg tcgaggagct ggcgcaggat attagatatc
     9721 cgaaggacgt tgacacattg gccaccagag tgaccagcgc caggcagttg aagaagtgca
     9781 gcactccggc ccgcagtccg atcatcggat aggcaatcgc cgtgaagacc agtggcactg
     9841 tgagaaaaag cggcaattcg gcaatcgttt tgcccagaaa gtatgtgtca cagcgataaa
     9901 gtcgacttcg ggcctccctc ataaaaactg gcagctctga ggtgaacacc taaatcgaat
     9961 cgattcatta gaaagttagt aaattattga aatgcaaatg tattctaaac atgacttaca
    10021 tttatcgtgg caaagacgtt ttgaaaggtc atgttggtca ggaagaggaa gatggctccg
    10081 ttgatattca tcacacccac ttgcgtgagt tgttggccca aaaagatgag gccaatcaag
    10141 atggcaacca tctgcaaatt aaaatgttac tcgcatctca ttaatattcg cgagttaaat
    10201 gaaatttatt tatcttctgc aaaactataa actatacatc tcattgaaaa aaactaagaa
    10261 gggtgtggaa tcaggcaatt ctatctaaaa tctagcgaat ttgtttccaa gaattgtaag
    10321 cgttatatca tttgtttcca ctggaa
//
