LOCUS       sequence-491105-       12070 bp    DNA     circular SYN 30-OCT-2025
DEFINITION  Overexpression of a bacterial bifunctional salicylate synthase, 
            Irp9, targeted to the chloroplast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12070)
  AUTHORS   Xue LJ, Guo W, Yuan Y, Anino EO, Nyamdari B, Wilson MC, Frost CJ, 
            Chen HY, Babst BA, Harding SA, Tsai CJ
  TITLE     Constitutively elevated salicylic acid levels alter photosynthesis 
            and oxidative state but not growth in transgenic populus.
  JOURNAL   Plant Cell. 2013 Jul;25(7):2714-30. doi: 10.1105/tpc.113.112839. 
            Epub 2013 Jul 31.
  PUBMED    23903318
REFERENCE   2  (bases 1 to 12070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 30, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.113.112839"; journalName: "Plant Cell"; date: 
            "2013-07"; volume: "25"; issue: "7"; pages: "2714-30"
COMMENT     Sequence Label: pCAMBIA1302-Pro35S:Fd-Irp9
FEATURES             Location/Qualifiers
     source          1..12070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(4997..5249)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5281..5298)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(5305..6015)
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(5345..5365)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     5960..5988
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(7568..7913)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(8147..8374)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     primer_bind     8275..8297
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8289..8306
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8290..8306
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8310..8366
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8375..8391)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8375..8391)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8388..8410)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8399..8415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8423..8429,8430..8447,8448..8453))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8468..8489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8680..9356
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             9423..10448
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    10489..10663
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10741..10765
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11190..11984
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta attcccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     5041 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     5101 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     5161 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
     5221 aaactttatt gccaaatgtt tgaacgatcg gggaaattcg agctggtcac caattcacac
     5281 gtggtggtgg tggtggtggc tagctttgta tagttcatcc atgccatgtg taatcccagc
     5341 agctgttaca aactcaagaa ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag
     5401 ggcagattgt gtggacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg
     5461 agtattttgt tgataatgat cagcgagttg cacgccgccg tcttcgatgt tgtggcgggt
     5521 cttgaagttg gctttgatgc cgttcttttg cttgtcggcc atgatgtata cgttgtggga
     5581 gttgtagttg tattccaact tgtggccgag gatgtttccg tcctccttga aatcgattcc
     5641 cttaagctcg atcctgttga cgagggtgtc tccctcaaac ttgacttcag cacgtgtctt
     5701 gtagttcccg tcgtccttga agaagatggt cctctcctgc acgtatccct caggcatggc
     5761 gctcttgaag aagtcgtgcc gcttcatatg atctgggtat cttgaaaagc attgaacacc
     5821 ataagagaaa gtagtgacaa gtgttggcca cggaacaggt agttttccag tagtgcaaat
     5881 aaatttaagg gtaagttttc cgtatgttgc atcaccttca ccctctccac tgacagaaaa
     5941 tttgtgccca ttaacatcac catctaattc aacaagaatt gggacaactc cagtgaaaag
     6001 ttcttctcct ttactagtaa cggccgccag tgtgctggaa ttcgcccttg ctagcctaca
     6061 ccattaaata gggcgcaatg ctcgctaatt tctcccgggt ttccgtcagt tcgcgttccg
     6121 gtgtagattg cgcgatgatt cccgcccccg cctgtatcca gcagcgctgg ctgtcctgaa
     6181 atacggaacg cagaactagc gccgcatcga agcgcgtatc gtccagcagc agaattgcgc
     6241 cggaataaag ctctcgcggc gtcttctcaa tttgcataat cgcgttcagg gcagcgtttt
     6301 tagggatacc tgaggcggta atcgacggaa acagcacggt aaacgcatcc caggcatcct
     6361 tgttttccgc aagctgaccg ctcacgccag accccagatg ctgaacgctg ccgcgctggc
     6421 gaaccgacat taaatcttca accaccacgc tgcccggcag gcaaacggcc tccagttcag
     6481 caatagcttc tttgacagaa aggatatgct caagcacctc tttactatcg tgcagcagtt
     6541 ctgcctcttt cgccttatta tgctccgggt ttcccatgcg atcgcgggtg cccgcaagcg
     6601 gttcagtaac cactttattg cccgtcactg acatcaccag ttccgggcta aagcccagcg
     6661 cttcgcgtcc ttcctgacgg aacataaacg agcgcactgg cgtgtttgcc tgtcgcccgt
     6721 ataacagcgt ggcgggcata tcaatccgcg atggcagggg aatggcgcgc gagacaatca
     6781 ctttgacata ctcgccacgg cggatctccg caacggcgcg cgcaacctgt tgtttatacg
     6841 cctcaccatt gagggcggta tccaccgcca gtggtgcgtt ctgcgtcgtt gtactggcct
     6901 cttttaccca ctcgcacaaa cgtcggcacc cgtcggcgga gtccgcataa acggtgacat
     6961 ttcccttttc aaaaataagt tcttcacggg gaacggttaa cgtcagcagc ggccactccc
     7021 cggcgttaaa ggcaatcccc cgcgcatgcg ccgcaaagtt gaacccaacc tgtccatata
     7081 cccgacgtcc tttcacctga ggatgtgcca taaaacgacg cgcgcagtcg gcaatggaat
     7141 ccaccgccca tttttgctcc ccggcatcat caataaatgt cgcctgcgtt ccgtcggcat
     7201 taatgtgcag ccgtgcctgg caccctttgc ctaaatacca acagggttgg cgctcataga
     7261 cataacactc ttcttctgcg aactggcgta aaacgccaga aatcgtcggc agccattgtt
     7321 cctctggtaa cgccaggtgt agaaattcac tgattttcat gtcgacgacc ttgtatgtag
     7381 ccatggctgt gacacgtcca ccacgagcgg tgcctgattt gagaccgaag agggattgtg
     7441 tgttggctga tggaagggaa cggagactga ttggagctgg ggaacgacgg atgaatgaag
     7501 ttccgacgat ggcgcttgag agagcagtgg aagccatttt agatctacca tggtcaagag
     7561 tcccccgtgt tctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
     7621 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
     7681 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     7741 ctttgggacc actgtcggca gaggcatctt caacgatggc ctttccttta tcgcaatgat
     7801 ggcatttgta ggagccacct tccttttcca ctatcttcac aataaagtga cagatagctg
     7861 ggcaatggaa tccgaggagg tttccggata ttaccctttg ttgaaaagtc tcaattgccc
     7921 tttggtcttc tgagactgta tctttgatat ttttggagta gacaagtgtg tcgtgctcca
     7981 ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa gagactctgt atgaactgtt
     8041 cgccagtctt tacggcgagt tctgttaggt cctctatttg aatctttgac tccatgaagc
     8101 taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctgctctag cattcgccat
     8161 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     8221 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     8281 cacgacgttg taaaacgacg gccagtgcca agcttgcatg cctgcaggtc gactctagag
     8341 gatccccggg taccgagctc gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt
     8401 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8461 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8521 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8581 tgcgtattgg ctagagcagc ttgccaacat ggtggagcac gacactctcg tctactccaa
     8641 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
     8701 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
     8761 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
     8821 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     8881 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg
     8941 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg
     9001 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
     9061 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc
     9121 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg
     9181 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     9241 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
     9301 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaaatc
     9361 accagtctct ctctacaaat ctatctctct cgagctttcg cagatccggg gggcaatgag
     9421 atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     9481 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     9541 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     9601 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     9661 ttggggagtt tagcgagagc ctgacctatt gcatctcccg ccgttcacag ggtgtcacgt
     9721 tgcaagacct gcctgaaacc gaactgcccg ctgttctaca accggtcgcg gaggctatgg
     9781 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     9841 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     9901 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     9961 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
    10021 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
    10081 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
    10141 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
    10201 cacgactccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
    10261 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
    10321 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
    10381 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
    10441 agaaatagag tagatgccga ccgggatctg tcgatcgaca agctcgagtt tctccataat
    10501 aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt
    10561 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
    10621 tttctaattc ctaaaaccaa aatccagtac taaaatccag atcccccgaa ttaattcggc
    10681 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
    10741 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
    10801 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
    10861 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
    10921 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
    10981 cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta
    11041 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
    11101 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
    11161 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
    11221 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
    11281 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
    11341 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
    11401 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
    11461 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
    11521 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
    11581 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
    11641 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
    11701 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
    11761 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
    11821 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
    11881 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
    11941 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gaatgcatga
    12001 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    12061 aaggatcttc
//
