LOCUS       sequence-49113-e         469 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 469)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FDX1
FEATURES             Location/Qualifiers
     source          1..469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             398..418
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             419..436
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             437..460
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttaagaagg agatatacta tgagcagctc agaagataaa ataacagtcc actttataaa
       61 ccgtgatggt gaaacattaa caaccaaagg aaaagttggt gattctctgc tagatgttgt
      121 ggttgaaaat aatctagata ttgatggctt tggtgcatgt gagggaaccc tggcttgttc
      181 aacctgtcac ctcatctttg aagatcacat atatgagaag ttagatgcaa tcactgatga
      241 ggagaatgac atgctcgatc tggcatatgg actaacagac agatcacggt tgggctgcca
      301 aatctgtttg acaaaatcta tggacaatat gactgttcga gtgcctgaaa cagtggctga
      361 tgccagacaa tccattgatg tgggcaagac ctccgcagag aacctctact tccaatcgca
      421 ccatcatcac caccatgatt acaaggatga cgacgataag tgaggatcc
//
