LOCUS       sequence-491172-       12794 bp    DNA     circular SYN 30-OCT-2025
DEFINITION  Evaluation of MtU6.6-104 promoter (Pol III promoter) deletion (104 
            bp) for CRISPR-Cas9 editing of mEGFP in Nicotiana benthamiana and 
            poplar reporter lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Deguchi M, Sinclair KM, Patel A, Coile M, Ortega MA, Bewg WP, Tsai 
            CJ
  TITLE     A compendium of nonredundant short polymerase III promoters for 
            CRISPR applications.
  JOURNAL   Plant Physiol. 2025 Jul 3;198(3):kiaf294. doi: 
            10.1093/plphys/kiaf294.
  PUBMED    40673482
REFERENCE   2  (bases 1 to 12794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 30, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-07-3"; volume: "198(3):kiaf294. doi"; pages: 
            " 10.1093/plphys/kiaf294"
COMMENT     Sequence Label: p305N-21
FEATURES             Location/Qualifiers
     source          1..12794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(829..1977)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2276..3070)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3282..3913
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    3993..4017
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(4532..5326)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4643..4662)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5253..5272
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_RNA        6388..6463
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             8147..12250
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8147..12250
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8147..12250
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      12290..12538
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    12557..12581
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag aggatctcag
      781 taaagcgctg gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca
      841 atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc
      901 gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat
      961 gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa
     1021 catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg
     1081 gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt
     1141 cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac
     1201 gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt
     1261 cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa
     1321 ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag
     1381 ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac
     1441 gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa
     1501 cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat
     1561 atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc
     1621 ttgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt
     1681 cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc
     1741 gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg
     1801 cacgctgtcg cgctcgatct tggccgtagc ttgctgggcc atcgagccga cggactggaa
     1861 ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga
     1921 aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca
     1981 ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga
     2041 tccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta
     2101 gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag
     2161 cgaccccttg cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg
     2221 gaagaagtcg gtgcgctcct gcttgtcgcc agcatcgttg cgccacatct aggtactaaa
     2281 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
     2341 agtcaaaaaa tagctcgaca tactgttctt ccccaatatc ctccctgatc gaccggacgc
     2401 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
     2461 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
     2521 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
     2581 gggtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
     2641 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
     2701 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
     2761 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
     2821 catcatgccg ttcaaggtgc aggacctttg gaacaggcag ctttccttcc agccatagca
     2881 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
     2941 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
     3001 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
     3061 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
     3121 taattataac aagacgaact ccaattcact gttccttgca ttctaaaact ttaaatacca
     3181 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
     3241 gattttgaaa ccacaattat gggtgatgct gccaacgcga gagcgggccg ggagggttcg
     3301 agaagggggg gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt
     3361 aaaaacaagg tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc
     3421 gaaaaacggg cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa
     3481 tgtcaatagg tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc
     3541 ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag
     3601 gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga
     3661 ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc
     3721 gccgggtgag tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa
     3781 gttttccgcg aggtatccac aacgccggcg gctgcggtgt ctcgcacacg gcttcgacgg
     3841 cgtttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag
     3901 cccggtgagc gtctctagtg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
     3961 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
     4021 ttgacgattt aaatggtagg cgcggcttaa ttaagcgctt aattaagcgc ttaattaagc
     4081 gcttaataag ccgcgccgta ccccaatagt ctcgacagac acatagcacc taacattatt
     4141 gcttaaattt actatttttt tactatatta tactcaaccc aatgagcata aagactgtaa
     4201 aatctcaaat tcctgagaag catatttatc gatcccacag acttgatagt tccataatcc
     4261 atacgctgca gccaaattgc tagtgtgttg aacatttaac acgtagagaa ctagaaaaga
     4321 tataaaacta agattgatat ccaaaataga cgagaacaat aagcaaaaac tcttagtttt
     4381 gaaataaatc aacaatcccg agggttgtca catacatcaa aaacgaaaat ccatatagca
     4441 aaaaaaactc taaattaccg ttcgacaaaa agagaaaact gataggacat ttgctaaaca
     4501 ttaaaatcaa aatttggatc aagccgaatt ctcagaagaa ctcgtcaaga aggcgataga
     4561 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     4621 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     4681 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     4741 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc atcgccgtcg ggcatgcgcg
     4801 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     4861 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     4921 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     4981 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     5041 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     5101 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg
     5161 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     5221 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     5281 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatctcg agacgtaccg
     5341 cgcgatgcat tcgaagatcg atccttcgcc tggaggagag aaatcagtgg cgctgcggct
     5401 tttagggttt ctttgttgat ggaatgagag tgtaagctct gccagtgcca ctttattagg
     5461 gttttacaag cccttttctt cgtaattggg cctgacattt tgtgccactt gggcctttag
     5521 agatgaaaat gtatattggg cttaagttga cttgaaggat aaattagttt aggatattac
     5581 gttttttatg agaattggtg tgtcggatac atgtatatga tgcattcaaa tatatgtatt
     5641 ctagatacat ttaagtttag atacaatcta aaatgtgtct ttaattacag gactgtaact
     5701 aaaatactta atgtaagaag aatattactc ctttaatagc ttttgagtat atctagtcta
     5761 acatctttta aaaaagtcta atttctttca tttatttttc gagcaatagc aaagtgcata
     5821 attatttttt tcttctagaa attcagattt gtttctctaa attttgagat tcttttctca
     5881 attttgtatg tctagagaac aatgtgtatt tttcactcta gttggttgtt actttgttga
     5941 atgttctgat aaaagtatat tgttatttct gaagtagata taaaccttca tttggaaatt
     6001 atacataaat caaaatcgtt aattatctag atcaagatat atgccctttt cctaatgtat
     6061 ttgatacatg cacctaattt cactagatgt atcttttcta ttttttaaat tatgaatagt
     6121 taattttttc catatgtgta tttgatacat acttcatgac tttaaaaaat taattatata
     6181 ccagatatat gtatttaaaa tttgttatgt atttaaagta tgtatatgat tattcgatat
     6241 taatctcttc gatgaaccta ggcgagaagg atttgcatgt tactattaca cttgcttttt
     6301 agtcccacat cgtctgaaac ataaaatatt tcagcgttta aatacttcaa gcgaaccagt
     6361 aggcttgcat gcccgaaggc tacgtccgtt ttagagctag aaatagcaag ttaaaataag
     6421 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt actagtggag
     6481 ctcaactttt gattcgctat ttgcagtgca cctgtggcgt tcatcacatc ttttgtgaca
     6541 ctgtttgcac tggtcattgc tattacaaag gaccttcctg atgttgaagg agatcgaaag
     6601 taagtaactg cacgcataac cattttcttt ccgctctttg gctcaatcca tttgacagtc
     6661 aaagacaatg tttaaccagc tccgtttgat atattgtctt tatgtgtttg ttcaagcatg
     6721 tttagttaat catgcctttg attgatcttg aataggttcc aaatatcaac cctggcaaca
     6781 aaacttggag tgagaaacat tgcattcctc ggttctggac ttctgctagt aaattatgtt
     6841 tcagccatat cactagcttt ctacatgcct caggtgaatt catctatttc cgtcttaact
     6901 atttcggtta attaaagcac gaacaccatt actgcatgta gaagcttgat aaactatcgc
     6961 caccaattta tttttgttgc gatattgtta ctttcctcag tatgcagctt tgaaaagacc
     7021 aaccctctta tcctttaaca atgaacaggt ttttagaggt agcttgatga ttcctgcaca
     7081 tgtgatcttg gcttcaggct taattttcca ggtaaagcat tatgagatac tcttatatct
     7141 cttacatact tttgagataa tgcacaagaa cttcataact atatgcttta gtttctgcat
     7201 ttgacactgc caaattcatt aatctctaat atctttgttg ttgatctttg gtagacatgg
     7261 gtactagaaa aagcaaacta caccaaggta aaatactttt gtacaaacat aaactcgtta
     7321 tcacggaaca tcaatggagt gtatatctaa cggagtgtag aaacatttga ttattgcagg
     7381 aagctatctc aggatattat cggtttatat ggaatctctt ctacgcagag tatctgttat
     7441 tccccttcct ctagctttca atttcatggt gaggatatgc agttttcttt gtatatcatt
     7501 cttcttcttc tttgtagctt ggagtcaaaa tcggttcctt catgtacata catcaaggat
     7561 atgtccttct gaatttttat atcttgcaat aaaaatgctt gtaccaattg aaacaccagc
     7621 tttttgagtt ctatgatcac tgacttggtt ctaaccaaaa aaaaaaaaat gtttaattta
     7681 catatctaaa agtaggttta gggaaaccta aacagtaaaa tatttgtata ttattcgaat
     7741 ttcactcatc ataaaaactt aaattgcacc ataaaatttt gttttactat taatgatgta
     7801 atttgtgtaa cttaagataa aaataatatt ccgtaagtta accggctaaa accacgtata
     7861 aaccagggaa cctgttaaac cggttcttta ctggataaag aaatgaaagc ccatgtagac
     7921 agctccatta gagcccaaac cctaaatttc tcatctatat aaaaggagtg acattagggt
     7981 ttttgttcgt cctcttaaag cttctcgttt tctctgccgt ctctctcatt cgcgcgacgc
     8041 aaacgatctt caggtgatct tctttctcca aatcctctct cataactctg atttcgtact
     8101 tgtgtatttg agctcacgct ctgtttctct caccacagca gctagcatgg acaagaagta
     8161 ctccattggg ctcgatatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta
     8221 caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa
     8281 gaacctcatt ggcgccctcc tgttcgactc cggggagacg gccgaagcca cgcggctcaa
     8341 aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat
     8401 ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt
     8461 tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga
     8521 ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag
     8581 tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg
     8641 gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt
     8701 tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg
     8761 agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct
     8821 catcgcacag ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc
     8881 actcgggctg acccccaact ttaaatctaa cttcgacctg gccgaagatg ccaagcttca
     8941 actgagcaaa gacacctacg atgatgatct cgacaatctg ctggcccaga tcggcgacca
     9001 gtacgcagac ctttttttgg cggcaaagaa cctgtcagac gccattctgc tgagtgatat
     9061 tctgcgagtg aacacggaga tcaccaaagc tccgctgagc gctagtatga tcaagcgcta
     9121 tgatgagcac caccaagact tgactttgct gaaggccctt gtcagacagc aactgcctga
     9181 gaagtacaag gaaattttct tcgatcagtc taaaaatggc tacgccggat acattgacgg
     9241 cggagcaagc caggaggaat tttacaaatt tattaagccc atcttggaaa aaatggacgg
     9301 caccgaggag ctgctggtaa agcttaacag agaagatctg ttgcgcaaac agcgcacttt
     9361 cgacaatgga agcatccccc accagattca cctgggcgaa ctgcacgcta tcctcaggcg
     9421 gcaagaggat ttctacccct ttttgaaaga taacagggaa aagattgaga aaatcctcac
     9481 atttcggata ccctactatg taggccccct cgcccgggga aattccagat tcgcgtggat
     9541 gactcgcaaa tcagaagaga ccatcactcc ctggaacttc gaggaagtcg tggataaggg
     9601 ggcctctgcc cagtccttca tcgaaaggat gactaacttt gataaaaatc tgcctaacga
     9661 aaaggtgctt cctaaacact ctctgctgta cgagtacttc acagtttata acgagctgac
     9721 caaggtcaaa tacgtcacag aagggatgag aaagccagca ttcctgtctg gagagcagaa
     9781 gaaagctatc gtggacctcc tcttcaagac gaaccggaaa gttaccgtga aacagctcaa
     9841 agaagactat ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg gagtggagga
     9901 tcgcttcaac gcatccctgg gaacgtatca cgatctcctg aaaatcatta aagacaagga
     9961 cttcctggac aatgaggaga acgaggacat tcttgaggac attgtcctca cccttacgtt
    10021 gtttgaagat agggagatga ttgaagaacg cttgaaaact tacgctcatc tcttcgacga
    10081 caaagtcatg aaacagctca agaggcgccg atatacagga tgggggcggc tgtcaagaaa
    10141 actgatcaat ggcatccgag acaagcagag tggaaagaca atcctggatt ttcttaagtc
    10201 cgatggattt gccaaccgga acttcatgca gttgatccat gatgactctc tcacctttaa
    10261 ggaggacatc cagaaagcac aagtttctgg ccagggggac agtcttcacg agcacatcgc
    10321 taatcttgca ggtagcccag ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga
    10381 tgaactcgtc aaagtaatgg gaaggcataa gcccgagaat atcgttatcg agatggcccg
    10441 agagaaccaa actacccaga agggacagaa gaacagtagg gaaaggatga agaggattga
    10501 agagggtata aaagaactgg ggtcccaaat ccttaaggaa cacccagttg aaaacaccca
    10561 gcttcagaat gagaagctct acctgtacta cctgcagaac ggcagggaca tgtacgtgga
    10621 tcaggaactg gacatcaatc ggctctccga ctacgacgtg gatcatatcg tgccccagtc
    10681 ttttctcaaa gatgattcta ttgataataa agtgttgaca agatccgata aaaatagagg
    10741 gaagagtgat aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt attggcggca
    10801 gctgctgaac gccaaactga tcacacaacg gaagttcgat aatctgacta aggctgaacg
    10861 aggtggcctg tctgagttgg ataaagccgg cttcatcaaa aggcagcttg ttgagacacg
    10921 ccagatcacc aagcacgtgg cccaaattct cgattcacgc atgaacacca agtacgatga
    10981 aaatgacaaa ctgattcgag aggtgaaagt tattactctg aagtctaagc tggtctcaga
    11041 tttcagaaag gactttcagt tttataaggt gagagagatc aacaattacc accatgcgca
    11101 tgatgcctac ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc ccaagcttga
    11161 atctgaattt gtttacggag actataaagt gtacgatgtt aggaaaatga tcgcaaagtc
    11221 tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt tacagcaata ttatgaattt
    11281 tttcaagacc gagattacac tggccaatgg agagattcgg aagcgaccac ttatcgaaac
    11341 aaacggagaa acaggagaaa tcgtgtggga caagggtagg gatttcgcga cagtccggaa
    11401 ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc gaagtacaga ccggaggctt
    11461 ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga
    11521 ttgggacccc aagaaatacg gcggattcga ttctcctaca gtcgcttaca gtgtactggt
    11581 tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg
    11641 catcacaatc atggagcgat caagcttcga aaaaaacccc atcgactttc tcgaagcgaa
    11701 aggatataaa gaggtcaaaa aagacctcat cattaagctt cccaagtact ctctctttga
    11761 gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga
    11821 gctggcactg ccctctaaat acgttaattt cttgtatctg gccagccact atgaaaagct
    11881 caaagggtct cccgaagata atgagcagaa gcagctgttc gtggaacaac acaaacacta
    11941 ccttgatgag atcatcgagc aaataagcga gttctccaaa agagtgatcc tcgccgacgc
    12001 taacctcgat aaggtgcttt ctgcttacaa taagcacagg gataagccca tcagggagca
    12061 ggcagaaaac attatccact tgtttactct gaccaacttg ggcgcgcctg cagccttcaa
    12121 gtacttcgac accaccatag acagaaagcg gtacacctct acaaaggagg tcctggacgc
    12181 cacactgatt catcagtcaa ttacggggct ctatgaaaca agaatcgacc tctctcagct
    12241 cggtggagac ggaacaagaa agagaagaaa ggtgtgacgg atcctcgaat atgaagatga
    12301 agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct
    12361 tggcttgttg tgttatgaat ttgtggcttt ttctaatatc aaatgaatgt aagatctcat
    12421 tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttctg
    12481 cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta aagataaggt
    12541 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
    12601 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
    12661 gtgcatgcca accacaggag atccaaaagg atctaggtga agatcctttt tgataatctc
    12721 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    12781 atcaaaggat cttc
//
