LOCUS       sequence-491197-       12756 bp    DNA     circular SYN 30-OCT-2025
DEFINITION  Evaluation of PtU6.2c4 promoter (nonfunctional) (Pol III promoter) 
            sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana 
            benthamiana reporter line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12756)
  AUTHORS   Deguchi M, Sinclair KM, Patel A, Coile M, Ortega MA, Bewg WP, Tsai 
            CJ
  TITLE     A compendium of nonredundant short polymerase III promoters for 
            CRISPR applications.
  JOURNAL   Plant Physiol. 2025 Jul 3;198(3):kiaf294. doi: 
            10.1093/plphys/kiaf294.
  PUBMED    40673482
REFERENCE   2  (bases 1 to 12756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 30, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-07-3"; volume: "198(3):kiaf294. doi"; pages: 
            " 10.1093/plphys/kiaf294"
COMMENT     Sequence Label: p305N-31 (nonfunctional)
FEATURES             Location/Qualifiers
     source          1..12756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(829..1977)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2276..3070)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3282..3913
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    3993..4017
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(4532..5326)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4643..4662)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5253..5272
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_RNA        6350..6425
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             8109..12212
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8109..12212
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8109..12212
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      12252..12500
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    12519..12543
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag aggatctcag
      781 taaagcgctg gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca
      841 atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc
      901 gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat
      961 gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa
     1021 catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg
     1081 gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt
     1141 cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac
     1201 gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt
     1261 cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa
     1321 ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag
     1381 ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac
     1441 gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa
     1501 cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat
     1561 atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc
     1621 ttgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt
     1681 cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc
     1741 gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg
     1801 cacgctgtcg cgctcgatct tggccgtagc ttgctgggcc atcgagccga cggactggaa
     1861 ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga
     1921 aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca
     1981 ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga
     2041 tccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta
     2101 gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag
     2161 cgaccccttg cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg
     2221 gaagaagtcg gtgcgctcct gcttgtcgcc agcatcgttg cgccacatct aggtactaaa
     2281 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
     2341 agtcaaaaaa tagctcgaca tactgttctt ccccaatatc ctccctgatc gaccggacgc
     2401 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
     2461 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
     2521 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
     2581 gggtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
     2641 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
     2701 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
     2761 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
     2821 catcatgccg ttcaaggtgc aggacctttg gaacaggcag ctttccttcc agccatagca
     2881 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
     2941 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
     3001 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
     3061 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
     3121 taattataac aagacgaact ccaattcact gttccttgca ttctaaaact ttaaatacca
     3181 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
     3241 gattttgaaa ccacaattat gggtgatgct gccaacgcga gagcgggccg ggagggttcg
     3301 agaagggggg gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt
     3361 aaaaacaagg tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc
     3421 gaaaaacggg cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa
     3481 tgtcaatagg tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc
     3541 ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag
     3601 gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga
     3661 ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc
     3721 gccgggtgag tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa
     3781 gttttccgcg aggtatccac aacgccggcg gctgcggtgt ctcgcacacg gcttcgacgg
     3841 cgtttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag
     3901 cccggtgagc gtctctagtg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
     3961 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
     4021 ttgacgattt aaatggtagg cgcggcttaa ttaagcgctt aattaagcgc ttaattaagc
     4081 gcttaataag ccgcgccgta ccccaatagt ctcgacagac acatagcacc taacattatt
     4141 gcttaaattt actatttttt tactatatta tactcaaccc aatgagcata aagactgtaa
     4201 aatctcaaat tcctgagaag catatttatc gatcccacag acttgatagt tccataatcc
     4261 atacgctgca gccaaattgc tagtgtgttg aacatttaac acgtagagaa ctagaaaaga
     4321 tataaaacta agattgatat ccaaaataga cgagaacaat aagcaaaaac tcttagtttt
     4381 gaaataaatc aacaatcccg agggttgtca catacatcaa aaacgaaaat ccatatagca
     4441 aaaaaaactc taaattaccg ttcgacaaaa agagaaaact gataggacat ttgctaaaca
     4501 ttaaaatcaa aatttggatc aagccgaatt ctcagaagaa ctcgtcaaga aggcgataga
     4561 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     4621 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     4681 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     4741 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc atcgccgtcg ggcatgcgcg
     4801 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     4861 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     4921 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     4981 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     5041 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     5101 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg
     5161 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     5221 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     5281 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatctcg agacgtaccg
     5341 cgcgatgcat tcgaagatcg atccttcgcc tggaggagag aaatcagtgg cgctgcggct
     5401 tttagggttt ctttgttgat ggaatgagag tgtaagctct gccagtgcca ctttattagg
     5461 gttttacaag cccttttctt cgtaattggg cctgacattt tgtgccactt gggcctttag
     5521 agatgaaaat gtatattggg cttaagttga cttgaaggat aaattagttt aggatattac
     5581 gttttttatg agaattggtg tgtcggatac atgtatatga tgcattcaaa tatatgtatt
     5641 ctagatacat ttaagtttag atacaatcta aaatgtgtct ttaattacag gactgtaact
     5701 aaaatactta atgtaagaag aatattactc ctttaatagc ttttgagtat atctagtcta
     5761 acatctttta aaaaagtcta atttctttca tttatttttc gagcaatagc aaagtgcata
     5821 attatttttt tcttctagaa attcagattt gtttctctaa attttgagat tcttttctca
     5881 attttgtatg tctagagaac aatgtgtatt tttcactcta gttggttgtt actttgttga
     5941 atgttctgat aaaagtatat tgttatttct gaagtagata taaaccttca tttggaaatt
     6001 atacataaat caaaatcgtt aattatctag atcaagatat atgccctttt cctaatgtat
     6061 ttgatacatg cacctaattt cactagatgt atcttttcta ttttttaaat tatgaatagt
     6121 taattttttc catatgtgta tttgatacat acttcatgac tttaaaaaat taattatata
     6181 ccagatatat gtatttaaaa tttgttatgt atttaaagta tgtatatgat tattcgatat
     6241 taatctcttc gatgaaggcc tagatatccc acatcgtctg gttagagaaa catgacgtat
     6301 aagtaagcga agcagagacc tgttagtcgc atgcccgaag gctacgtccg ttttagagct
     6361 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     6421 ggtgcttttt ttactagtgg agctcaactt ttgattcgct atttgcagtg cacctgtggc
     6481 gttcatcaca tcttttgtga cactgtttgc actggtcatt gctattacaa aggaccttcc
     6541 tgatgttgaa ggagatcgaa agtaagtaac tgcacgcata accattttct ttccgctctt
     6601 tggctcaatc catttgacag tcaaagacaa tgtttaacca gctccgtttg atatattgtc
     6661 tttatgtgtt tgttcaagca tgtttagtta atcatgcctt tgattgatct tgaataggtt
     6721 ccaaatatca accctggcaa caaaacttgg agtgagaaac attgcattcc tcggttctgg
     6781 acttctgcta gtaaattatg tttcagccat atcactagct ttctacatgc ctcaggtgaa
     6841 ttcatctatt tccgtcttaa ctatttcggt taattaaagc acgaacacca ttactgcatg
     6901 tagaagcttg ataaactatc gccaccaatt tatttttgtt gcgatattgt tactttcctc
     6961 agtatgcagc tttgaaaaga ccaaccctct tatcctttaa caatgaacag gtttttagag
     7021 gtagcttgat gattcctgca catgtgatct tggcttcagg cttaattttc caggtaaagc
     7081 attatgagat actcttatat ctcttacata cttttgagat aatgcacaag aacttcataa
     7141 ctatatgctt tagtttctgc atttgacact gccaaattca ttaatctcta atatctttgt
     7201 tgttgatctt tggtagacat gggtactaga aaaagcaaac tacaccaagg taaaatactt
     7261 ttgtacaaac ataaactcgt tatcacggaa catcaatgga gtgtatatct aacggagtgt
     7321 agaaacattt gattattgca ggaagctatc tcaggatatt atcggtttat atggaatctc
     7381 ttctacgcag agtatctgtt attccccttc ctctagcttt caatttcatg gtgaggatat
     7441 gcagttttct ttgtatatca ttcttcttct tctttgtagc ttggagtcaa aatcggttcc
     7501 ttcatgtaca tacatcaagg atatgtcctt ctgaattttt atatcttgca ataaaaatgc
     7561 ttgtaccaat tgaaacacca gctttttgag ttctatgatc actgacttgg ttctaaccaa
     7621 aaaaaaaaaa atgtttaatt tacatatcta aaagtaggtt tagggaaacc taaacagtaa
     7681 aatatttgta tattattcga atttcactca tcataaaaac ttaaattgca ccataaaatt
     7741 ttgttttact attaatgatg taatttgtgt aacttaagat aaaaataata ttccgtaagt
     7801 taaccggcta aaaccacgta taaaccaggg aacctgttaa accggttctt tactggataa
     7861 agaaatgaaa gcccatgtag acagctccat tagagcccaa accctaaatt tctcatctat
     7921 ataaaaggag tgacattagg gtttttgttc gtcctcttaa agcttctcgt tttctctgcc
     7981 gtctctctca ttcgcgcgac gcaaacgatc ttcaggtgat cttctttctc caaatcctct
     8041 ctcataactc tgatttcgta cttgtgtatt tgagctcacg ctctgtttct ctcaccacag
     8101 cagctagcat ggacaagaag tactccattg ggctcgatat cggcacaaac agcgtcggct
     8161 gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata
     8221 ccgatcgcca cagcataaag aagaacctca ttggcgccct cctgttcgac tccggggaga
     8281 cggccgaagc cacgcggctc aaaagaacag cacggcgcag atatacccgc agaaagaatc
     8341 ggatctgcta cctgcaggag atctttagta atgagatggc taaggtggat gactctttct
     8401 tccataggct ggaggagtcc tttttggtgg aggaggataa aaagcacgag cgccacccaa
     8461 tctttggcaa tatcgtggac gaggtggcgt accatgaaaa gtacccaacc atatatcatc
     8521 tgaggaagaa gcttgtagac agtactgata aggctgactt gcggttgatc tatctcgcgc
     8581 tggcgcatat gatcaaattt cggggacact tcctcatcga gggggacctg aacccagaca
     8641 acagcgatgt cgacaaactc tttatccaac tggttcagac ttacaatcag cttttcgaag
     8701 agaacccgat caacgcatcc ggagttgacg ccaaagcaat cctgagcgct aggctgtcca
     8761 aatcccggcg gctcgaaaac ctcatcgcac agctccctgg ggagaagaag aacggcctgt
     8821 ttggtaatct tatcgccctg tcactcgggc tgacccccaa ctttaaatct aacttcgacc
     8881 tggccgaaga tgccaagctt caactgagca aagacaccta cgatgatgat ctcgacaatc
     8941 tgctggccca gatcggcgac cagtacgcag accttttttt ggcggcaaag aacctgtcag
     9001 acgccattct gctgagtgat attctgcgag tgaacacgga gatcaccaaa gctccgctga
     9061 gcgctagtat gatcaagcgc tatgatgagc accaccaaga cttgactttg ctgaaggccc
     9121 ttgtcagaca gcaactgcct gagaagtaca aggaaatttt cttcgatcag tctaaaaatg
     9181 gctacgccgg atacattgac ggcggagcaa gccaggagga attttacaaa tttattaagc
     9241 ccatcttgga aaaaatggac ggcaccgagg agctgctggt aaagcttaac agagaagatc
     9301 tgttgcgcaa acagcgcact ttcgacaatg gaagcatccc ccaccagatt cacctgggcg
     9361 aactgcacgc tatcctcagg cggcaagagg atttctaccc ctttttgaaa gataacaggg
     9421 aaaagattga gaaaatcctc acatttcgga taccctacta tgtaggcccc ctcgcccggg
     9481 gaaattccag attcgcgtgg atgactcgca aatcagaaga gaccatcact ccctggaact
     9541 tcgaggaagt cgtggataag ggggcctctg cccagtcctt catcgaaagg atgactaact
     9601 ttgataaaaa tctgcctaac gaaaaggtgc ttcctaaaca ctctctgctg tacgagtact
     9661 tcacagttta taacgagctg accaaggtca aatacgtcac agaagggatg agaaagccag
     9721 cattcctgtc tggagagcag aagaaagcta tcgtggacct cctcttcaag acgaaccgga
     9781 aagttaccgt gaaacagctc aaagaagact atttcaaaaa gattgaatgt ttcgactctg
     9841 ttgaaatcag cggagtggag gatcgcttca acgcatccct gggaacgtat cacgatctcc
     9901 tgaaaatcat taaagacaag gacttcctgg acaatgagga gaacgaggac attcttgagg
     9961 acattgtcct cacccttacg ttgtttgaag atagggagat gattgaagaa cgcttgaaaa
    10021 cttacgctca tctcttcgac gacaaagtca tgaaacagct caagaggcgc cgatatacag
    10081 gatgggggcg gctgtcaaga aaactgatca atggcatccg agacaagcag agtggaaaga
    10141 caatcctgga ttttcttaag tccgatggat ttgccaaccg gaacttcatg cagttgatcc
    10201 atgatgactc tctcaccttt aaggaggaca tccagaaagc acaagtttct ggccaggggg
    10261 acagtcttca cgagcacatc gctaatcttg caggtagccc agctatcaaa aagggaatac
    10321 tgcagaccgt taaggtcgtg gatgaactcg tcaaagtaat gggaaggcat aagcccgaga
    10381 atatcgttat cgagatggcc cgagagaacc aaactaccca gaagggacag aagaacagta
    10441 gggaaaggat gaagaggatt gaagagggta taaaagaact ggggtcccaa atccttaagg
    10501 aacacccagt tgaaaacacc cagcttcaga atgagaagct ctacctgtac tacctgcaga
    10561 acggcaggga catgtacgtg gatcaggaac tggacatcaa tcggctctcc gactacgacg
    10621 tggatcatat cgtgccccag tcttttctca aagatgattc tattgataat aaagtgttga
    10681 caagatccga taaaaataga gggaagagtg ataacgtccc ctcagaagaa gttgtcaaga
    10741 aaatgaaaaa ttattggcgg cagctgctga acgccaaact gatcacacaa cggaagttcg
    10801 ataatctgac taaggctgaa cgaggtggcc tgtctgagtt ggataaagcc ggcttcatca
    10861 aaaggcagct tgttgagaca cgccagatca ccaagcacgt ggcccaaatt ctcgattcac
    10921 gcatgaacac caagtacgat gaaaatgaca aactgattcg agaggtgaaa gttattactc
    10981 tgaagtctaa gctggtctca gatttcagaa aggactttca gttttataag gtgagagaga
    11041 tcaacaatta ccaccatgcg catgatgcct acctgaatgc agtggtaggc actgcactta
    11101 tcaaaaaata tcccaagctt gaatctgaat ttgtttacgg agactataaa gtgtacgatg
    11161 ttaggaaaat gatcgcaaag tctgagcagg aaataggcaa ggccaccgct aagtacttct
    11221 tttacagcaa tattatgaat tttttcaaga ccgagattac actggccaat ggagagattc
    11281 ggaagcgacc acttatcgaa acaaacggag aaacaggaga aatcgtgtgg gacaagggta
    11341 gggatttcgc gacagtccgg aaggtcctgt ccatgccgca ggtgaacatc gttaaaaaga
    11401 ccgaagtaca gaccggaggc ttctccaagg aaagtatcct cccgaaaagg aacagcgaca
    11461 agctgatcgc acgcaaaaaa gattgggacc ccaagaaata cggcggattc gattctccta
    11521 cagtcgctta cagtgtactg gttgtggcca aagtggagaa agggaagtct aaaaaactca
    11581 aaagcgtcaa ggaactgctg ggcatcacaa tcatggagcg atcaagcttc gaaaaaaacc
    11641 ccatcgactt tctcgaagcg aaaggatata aagaggtcaa aaaagacctc atcattaagc
    11701 ttcccaagta ctctctcttt gagcttgaaa acggccggaa acgaatgctc gctagtgcgg
    11761 gcgagctgca gaaaggtaac gagctggcac tgccctctaa atacgttaat ttcttgtatc
    11821 tggccagcca ctatgaaaag ctcaaagggt ctcccgaaga taatgagcag aagcagctgt
    11881 tcgtggaaca acacaaacac taccttgatg agatcatcga gcaaataagc gagttctcca
    11941 aaagagtgat cctcgccgac gctaacctcg ataaggtgct ttctgcttac aataagcaca
    12001 gggataagcc catcagggag caggcagaaa acattatcca cttgtttact ctgaccaact
    12061 tgggcgcgcc tgcagccttc aagtacttcg acaccaccat agacagaaag cggtacacct
    12121 ctacaaagga ggtcctggac gccacactga ttcatcagtc aattacgggg ctctatgaaa
    12181 caagaatcga cctctctcag ctcggtggag acggaacaag aaagagaaga aaggtgtgac
    12241 ggatcctcga atatgaagat gaagatgaaa tatttggtgt gtcaaataaa aagcttgtgt
    12301 gcttaagttt gtgttttttt cttggcttgt tgtgttatga atttgtggct ttttctaata
    12361 tcaaatgaat gtaagatctc attataatga ataaacaaat gtttctataa tccattgtga
    12421 atgttttgtt ggatctcttc tgcagcatat aactactgta tgtgctatgg tatggactat
    12481 ggaatatgat taaagataag gtttaaacta tcagtgtttg acaggatata ttggcgggta
    12541 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
    12601 ttatccgttc gtccatttgt atgtgcatgc caaccacagg agatccaaaa ggatctaggt
    12661 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    12721 agcgtcagac cccgtagaaa agatcaaagg atcttc
//
