LOCUS       sequence-491249-        8585 bp    DNA     circular SYN 31-OCT-2025
DEFINITION  Expression plasmid for 3C-mCherry-TEV-mVenus-tagged proteins with 
            FAST-Red transgenic seed selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8585)
  TITLE     Kunz Lab Plant Expression Plasmids
REFERENCE   2  (bases 1 to 8585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 31, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pG20_3C_mCherry_TEV_mVenus_FR
FEATURES             Location/Qualifiers
     source          1..8585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      63..498
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    625..647
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             complement(1097..1792)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     protein_bind    3770..3791
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3806..3811,3812..3829,3830..3836)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3844..3860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3878..3889
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3896..4573
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      4618..4689
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4705..4732
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             4760..4783
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4790..5494
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4932..4950)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             5504..5524
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(5528..5530,5531..5533,5534..6247)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5573..5594)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5834..5853)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6181..6202
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6668..6692
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6783..7371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6863..6882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7542..8357)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7542..8357)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8265..8284
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8349..8368
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
      481 ttgcccaccg tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta
      541 taaatttttt tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga
      601 tttccgatcc ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcactaaa
      661 tggagcaacc tactgttttt gaagtttggg ttgccatgta atcacttgca ataaatatat
      721 gtactaatga ctgcatcggc ttgcgtgtct aaatttgtag cttattgaaa actttttgtg
      781 gttaaagacg aatccagtta actaacataa gtttaacaaa acaagacctt ttccatatat
      841 agaccaccgt taccaactta caaagaagcc aaataaagta caacacaaca cttgcaagtt
      901 acaaaaagag aggaaaaaaa acatggtacc atttgacaac atatagaaac acacataaac
      961 attacttata acaaaaagat tcaaactcgg cttttattat ctccccctga tcaaattcta
     1021 aacactttat tcctcataaa ctaactcccc gacattggag ttattggact atgacgatga
     1081 catcagtggg gtaagctcac ttgtgcccca gtttgctagg gaggtcgcag tatctggcca
     1141 cagccacctc gtgctgctcg acgtaggttt ctttgtcggc ctccttgatt ctttccagtc
     1201 tgtggtccac atagtagacg ccgggcatct tgaggttctt agcgggtttc ttggatctgt
     1261 atgtggtctt gaagttgcag atcaggtggc ccccgcccac gagcttcagg gccatgtcgc
     1321 ttctgccttc caggccgccg tcagcggggt acagcatctc ggtgttggcc tcccagccga
     1381 gtgttttctt ctgcatcaca gggccgttgg atgggaagtt cacccctctg atcttgacgt
     1441 tgtagatgag gcagccgtcc tggaggctgg tgtcctgggt agcggtcagc acgcccccat
     1501 cttcgtatgt ggtgactctc tcccatgtga agccctcagg gaaggactgc ttaaagaagt
     1561 cggggatgcc ctgggtgtgg ttgatgaagg ttctgctgcc gtacatgaag ctggtagcca
     1621 ggatgtcgaa ggcgaagggg agagggccgc cctcgaccac cttgattctc atggtctggg
     1681 tgccctcgta gggcttgcct tcgccctcgg atgtgcactt gaagtggtgg ttgttcacgg
     1741 tgccctccat gtacagcttc atgtgcatgt tctccttaat cagctcgctc atagtagtgt
     1801 gctggccacc acgagtacgg tcacggtcat gttccccacc agtatgttgc tgtccgtagt
     1861 actgagctct gtctttcaga tcctgagctt tgcttcccaa cttcatcctt gcactgtcca
     1921 acttgtctga tccctgtggg tgctctcccg ttgcgtacct attcacaatc aataggtatc
     1981 ataccaagca ttttacctat atagatgata tacacagaca cattcaagct agctaggtca
     2041 aatttcgtat ttagttaacc aaatgttccc tagaatttgc aatttcttat tgttacattt
     2101 gttattcgac caaaaaaatt gatccaaagc tactggctca acacatgcat gcacatattg
     2161 cacaaaatta tgaagtaaga tgataaatgt gtgcttactt gtaaatccaa gagaaaacgg
     2221 ttatagcggc aatgccaaac cctccagagg aaagaaaacc ggtgatgagg agtgcaactg
     2281 tgatgagagc cgggacaagg attgggctga agataacgag cagaggtgtt gcaacagtca
     2341 aagctatgac agttccaaca agggtaaggc tggagagaac aaggagggaa ccaccagctg
     2401 tgacagcagt tgcagcttta gcaatctgcc tagacttgga gtagtcagat cctcgtccgg
     2461 acatctggta ctggtcacga tctcggccca tcatcgggta ctgatctctg ccgatgatat
     2521 cgtgatgggt tcctctagct gtatccgcca tttttttgtt cttgtttact agagagaatg
     2581 agattttgaa tgagagattg atcaaaaaga tgtgtatata ttttggaaga ggtaggagtg
     2641 tttgttagat atattaagaa aggagacgag aagtcacgtg tcaatggaga catcatgcaa
     2701 ttgtaggcca cgtgttaatg gcgtcaccat gcagccgctg catgtgttaa cgagaggagt
     2761 ctattcaaag acacgtttgt tttttgccta gctcagaatg tggcgttggc atcgaaccga
     2821 aacataaaac gcaaaacgaa cctttgggct cgtcagtatg ggtttgggcc ttcaatagat
     2881 tccttctata cggcccacat atccactatt ggcaacaaaa ttagaaaaat ttaggattta
     2941 gttttaaaat ttgttccgcg acattataaa gcttcgacga gtcagtaata aacggcgtca
     3001 aagtggttgc agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc
     3061 ctcaacctaa aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata
     3121 cacgtgtcat tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg
     3181 tcctcgtctt ttcttcttct tcttctataa aacaataccc aaagacctct tcttcttcac
     3241 aattcagatt tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat
     3301 caaggtaaat ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc
     3361 ccaatttcgt atatgttctt tggtttagat tctgttaatc ttagatcgaa gtcgattttc
     3421 tgggtttgat cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga
     3481 tttgttcaaa taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta
     3541 gatctggtgt tagtttctag tttgtgcgat cgaatttgtc gattaatctg agtttttctg
     3601 attaacagac tagagttaac cgggctcagg cctggcgcgc cactagtgga tccgatggtg
     3661 tcttcgattc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3721 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     3781 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     3841 gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat actagatggc
     3901 ttcctccgag gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
     3961 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
     4021 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
     4081 cccgcagttc cagtacggtt ccaaggctta cgttaaacac ccggctgaca tcccggacta
     4141 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaggacgg
     4201 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
     4261 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaacgatggg
     4321 ttgggaagca tccaccgaac gtatgtaccc ggaggacggt gctctgaaag gtgaaatcaa
     4381 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
     4441 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
     4501 ctcccacaac gaggactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     4561 caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa
     4621 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     4681 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttataga
     4741 agacactgga cccgggagtc tggaagttct gttccagggg ccctccgggg tgagcaaggg
     4801 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     4861 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     4921 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     4981 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     5041 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     5101 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     5161 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaaaac
     5221 gatgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     5281 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     5341 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     5401 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     5461 ccactccacc ggcggcatgg acgagctgta caagtccggg agtgagaacc tctacttcca
     5521 aggctccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     5581 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     5641 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
     5701 gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca
     5761 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     5821 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     5881 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     5941 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
     6001 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
     6061 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     6121 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     6181 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     6241 caagtaagag ctcatatgaa gatgaagatg aaatatttgg tgtgtcaaat aaatagcttg
     6301 tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg gctttttcta
     6361 atattaaatg aatgtaagat ctcattataa tgaataaaca aatgtttcta taatccattg
     6421 tgaatgtttt gttggatctc ttctgcagca tataactact gtatgtgcta tggtatggac
     6481 tatggaatat gattaaagat aaggaattca gatcggctga gtggctcctt caacgttgcg
     6541 gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt
     6601 gactccctta attctccgct catgatcaga ttgtcgtttc ccgccttcag tttaaactat
     6661 cagtgtttga caggatatat tggcgggtaa actaagtcgc tgtatgtgtt tgtttgagat
     6721 ctcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7201 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7261 accttcggaa gaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7441 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7501 aaatcaatct aaagtatata tgtgtaacat tggtctagtg attagaaaaa ctcatcgagc
     7561 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     7621 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     7681 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     7741 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     7801 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     7861 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     7921 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     7981 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     8041 gctgttttcc ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     8101 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     8161 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     8221 ttcccataca atcggtagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     8281 tacccatata aatcagcatc catgttggaa tttaatcgcg gccttgagca agacgtttcc
     8341 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     8401 gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg
     8461 tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc
     8521 gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac
     8581 aacaa
//
