LOCUS       sequence-491259-        7387 bp    DNA     circular SYN 31-OCT-2025
DEFINITION  Genetically encoded voltage indicator (GEVI) JEDI-2Psub expressed 
            under strong mammalian promoter (CAG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7387)
  TITLE     All-optical voltage interrogation for probing synaptic plasticity in
            vivo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 31, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1/Puro-CAG-JEDI-2Psub
FEATURES             Location/Qualifiers
     source          1..7387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    83..204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(120..139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     174..193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(253..269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(253..269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(266..288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    277..293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(301..307,308..325,326..331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    346..367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(484..501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(655..1243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(735..754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1414..2205,2206..2274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2037..2056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2275..2379)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        2411..2790
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2792..3069
                     /label=chicken beta-actin promoter
     intron          3070..4086
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4010..4032
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4094..4113
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4246..4255
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5653..5670)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5659..5883
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5929..6357
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6016..6035)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6226..6247
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6366..6386)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6371..6700
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6551..6686
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6613..6632
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6711..7310
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6711..6730)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7207..7227
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
       61 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
      121 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      181 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
      241 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      301 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      361 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      421 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      481 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      541 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      601 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      661 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      721 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      781 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      841 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      901 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      961 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1021 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1081 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1141 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1201 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1261 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1321 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1381 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1441 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1501 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1561 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1621 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1681 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1741 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1801 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1861 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1921 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1981 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2041 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2101 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2161 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2221 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2281 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2341 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2401 acctgacgtc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2461 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2521 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2581 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2641 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2701 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2761 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     2821 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     2881 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc cagggggggg gggggggggg
     2941 gagggggggg gsggggsgag gsggagaggt gsggcggcag ccaatcagag cggcgcgctc
     3001 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     3061 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     3121 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     3181 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     3241 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     3301 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     3361 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     3421 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     3481 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     3541 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     3601 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     3661 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     3721 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     3781 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     3841 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     3901 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     3961 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     4021 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     4081 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc
     4141 ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     4201 aatgccctgg ctcacaaata ccactgagat ctgcatgagc tagccgccac catggagacg
     4261 actgtgaggt atgaacaggg gtcagagctc actaaaactt cgagctctcc aacagcagat
     4321 gagcccacga taaagattga tgatggtcgt gatgagggta atgaacaaga cagctgttcc
     4381 aataccatta ggagaaaaat ttccccgttt gtgatgtcat ttggattcag agtatttgga
     4441 gttgtgctta tcattgtaga catcatagtg gtgattgtgg atctggccat cagtgagaag
     4501 aaaagaggca ttagagagat tcttgaaggt gtttccctgg ctatagcact cttcttcctt
     4561 gttgatgttc tcatgagagt gtttgttgaa ggcttcaaga actatttccg gtccaaactg
     4621 gttactttgg atgcagtcat agtagtgggc actctgctaa ttaatatgac ctactccttc
     4681 tctgaccttg ctgcctttaa cagcgagaac gtgtatatta ccgcggataa acagaaaaac
     4741 ggcattaaag cgaactttac cgtgcgccat aacgtggaag atggcagcgt gcagctggcg
     4801 gatcattatc agcagaacac cccgattggc gatggcccgg tgctgctgcc ggataaccat
     4861 tatctgagcc accagaccgt gctgagcaaa gatccgaacg aaaaacgcga tcacatggtg
     4921 ctgctggaat ttgtgaccgc agcgggcatt acacacggca tggatgaact gtatggcggc
     4981 accggcggca gcgcgagcca gggcgaagaa ctgtttaccg gcgtggtgcc gattctggtg
     5041 gaactggatg gcgatgtgaa cggccataaa tttagcgtgc gcggcgaagg cgaaggcgat
     5101 gcgaccattg gcaaactgac cctgaaattt atttgcacca ccggcaaact accggtgccg
     5161 tggccgaccc tggtgaccac cttaacctat ggcgtgcagt gctttagccg ctatccggat
     5221 catatgaaac gccatgattt ttttaaaagc gcgatgccgg aaggctatgt gcaggaacgc
     5281 accattagct ttaaagatga tggcaaatat aaaacccgcg cggtggtgaa atttgaaggc
     5341 gataccctgg tgaaccgcat tgaactgaaa ggcaccgatt ttaaagaaga tggcaacatt
     5401 ctggggcata aactggaata tgattggaca gatcagatgc cgcacatggt tactcttctt
     5461 cgagttctga aaattgttat cttaataaga atatttcgcc tggcttcaca gaagaaacaa
     5521 cttgaagtgg taacctaatg aaagcttggt accgagctcg gatccactag tccagtgtgg
     5581 tggaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa
     5641 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     5701 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     5761 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     5821 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     5881 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     5941 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6001 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6061 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6121 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6181 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6241 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6301 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     6361 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6421 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     6481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6541 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6601 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     6661 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgc atgaccgagt
     6721 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     6781 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     6841 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     6901 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     6961 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     7021 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     7081 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     7141 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     7201 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     7261 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ttcgaaatga
     7321 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     7381 aaaggtt
//
