LOCUS       sequence-491261-        6879 bp    DNA     circular SYN 31-OCT-2025
DEFINITION  Soma and AIS-localized genetically encoded voltage indicator (GEVI) 
            JEDI-2Psub in AAV production vector for expression in excitatory 
            glutamatergic neurons using the promoter CaMKIIa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6879)
  TITLE     All-optical voltage interrogation for probing synaptic plasticity in
            vivo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 31, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CaMKIIa-JEDI-2Psub-Kv-WPRE
FEATURES             Location/Qualifiers
     source          1..6879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        149..1437
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      1458..1467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3006..3594
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3059..3079)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3477..3488)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3626..4102
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3825..3844)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4142..4282
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4142..4271
                     /label=AAV2 ITR
     rep_origin      4357..4812
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4444..4463)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4654..4675
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4829..4848)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4948..4970
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5008..5026)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5094..5198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5199..5267,5268..6059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5417..5436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6230..6818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6719..6738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttaaca ttatggcctt aggtcacttc atctccatgg
      181 ggttcttctt ctgattttct agaaaatgag atgggggtgc agagagcttc ctcagtgacc
      241 tgcccagggt cacatcagaa atgtcagagc tagaacttga actcagatta ctaatcttaa
      301 attccatgcc ttgggggcat gcaagtacga tatacagaag gagtgaactc attagggcag
      361 atgaccaatg agtttaggaa agaagagtcc agggcagggt acatctacac cacccgccca
      421 gccctgggtg agtccagcca cgttcacctc attatagttg cctctctcca gtcctacctt
      481 gacgggaagc acaagcagaa actgggacag gagccccagg agaccaaatc ttcatggtcc
      541 ctctgggagg atgggtgggg agagctgtgg cagaggcctc aggaggggcc ctgctgctca
      601 gtggtgacag ataggggtga gaaagcagac agagtcattc cgtcagcatt ctgggtctgt
      661 ttggtacttc ttctcacgct aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg
      721 agagctggaa agaaacaagg acagagacag aggccaagtc aaccagacca attcccagag
      781 gaagcaaaga aaccattaca gagactacaa gggggaaggg aaggagagat gaattagctt
      841 cccctgtaaa ccttagaacc cagctgttgc cagggcaacg gggcaatacc tgtctcttca
      901 gaggagatga agttgccagg gtaactacat cctgtctttc tcaaggacca tcccagaatg
      961 tggcacccac tagccgttac catagcaact gcctctttgc cccacttaat cccatcccgt
     1021 ctgttaaaag ggccctatag ttggaggtgg gggaggtagg aagagcgatg atcacttgtg
     1081 gactaagttt gttcgcatcc ccttctccaa ccccctcagt acatcaccct gggggaacag
     1141 ggtccacttg ctcctgggcc cacacagtcc tgcagtattg tgtatataag gccagggcaa
     1201 agaggagcag gttttaaagt gaaaggcagg caggtgttgg ggaggcagtt accggggcaa
     1261 cgggaacagg gcgtttcgga ggtggttgcc atggggacct ggatgctgac gaaggctcgc
     1321 gaggctgtga gcagccacag tgccctgctc agaagcccca agctcgtcag tcaagccggt
     1381 tctccgtttg cactcaggag cacgggcagg cgagtggccc ctagttctgg gggcagctct
     1441 agagcggtac cggatccgcc accatggaga cgactgtgag gtatgaacag gggtcagagc
     1501 tcactaaaac ttcgagctct ccaacagcag atgagcccac gataaagatt gatgatggtc
     1561 gtgatgaggg taatgaacaa gacagctgtt ccaataccat taggagaaaa atttccccgt
     1621 ttgtgatgtc atttggattc agagtatttg gagttgtgct tatcattgta gacatcatag
     1681 tggtgattgt ggatctggcc atcagtgaga agaaaagagg cattagagag attcttgaag
     1741 gtgtttccct ggctatagca ctcttcttcc ttgttgatgt tctcatgaga gtgtttgttg
     1801 aaggcttcaa gaactatttc cggtccaaac tggttacttt ggatgcagtc atagtagtgg
     1861 gcactctgct aattaatatg acctactcct tctctgacct tgctgccttt aacagcgaga
     1921 acgtgtatat taccgcggat aaacagaaaa acggcattaa agcgaacttt accgtgcgcc
     1981 ataacgtgga agatggcagc gtgcagctgg cggatcatta tcagcagaac accccgattg
     2041 gcgatggccc ggtgctgctg ccggataacc attatctgag ccaccagacc gtgctgagca
     2101 aagatccgaa cgaaaaacgc gatcacatgg tgctgctgga atttgtgacc gcagcgggca
     2161 ttacacacgg catggatgaa ctgtatggcg gcaccggcgg cagcgcgagc cagggcgaag
     2221 aactgtttac cggcgtggtg ccgattctgg tggaactgga tggcgatgtg aacggccata
     2281 aatttagcgt gcgcggcgaa ggcgaaggcg atgcgaccat tggcaaactg accctgaaat
     2341 ttatttgcac caccggcaaa ctaccggtgc cgtggccgac cctggtgacc accttaacct
     2401 atggcgtgca gtgctttagc cgctatccgg atcatatgaa acgccatgat ttttttaaaa
     2461 gcgcgatgcc ggaaggctat gtgcaggaac gcaccattag ctttaaagat gatggcaaat
     2521 ataaaacccg cgcggtggtg aaatttgaag gcgataccct ggtgaaccgc attgaactga
     2581 aaggcaccga ttttaaagaa gatggcaaca ttctggggca taaactggaa tatgattgga
     2641 cagatcagat gccgcacatg gttactcttc ttcgagttct gaaaattgtt atcttaataa
     2701 gaatatttcg cctggcttca cagaagaaac aacttgaagt ggtaaccggt tcttcaggtt
     2761 catctggatc ttccacgcgt cagtcccagc ccatcctcaa caccaaggag atggccccgc
     2821 agagcaagcc tccagaggag ctggagatga gcagcatgcc cagccccgtg gcccctctgc
     2881 ccgcacgcac ggagggcgtc atcgacatgc ggagcatgtc cagcattgac agcttcatca
     2941 gctgtgccac ggacttccct gaagccacca gattctgata agaattcgat atcaagctta
     3001 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3061 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3121 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3181 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3241 ccactggttg gggcattgcc accacctgtc agctcctttc cggaactttc gctttccccc
     3301 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3361 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     3421 tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3481 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3541 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     3601 accgagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct
     3661 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg
     3721 catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg
     3781 gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag
     3841 ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat
     3901 tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta
     3961 atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc
     4021 ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac
     4081 cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg
     4141 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4201 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4261 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4321 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4381 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     4441 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4501 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4561 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4621 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4681 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     4741 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4801 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4861 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4921 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4981 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5041 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5101 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5161 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5221 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5281 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5341 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5401 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5461 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5521 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5581 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5641 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5701 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5761 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5821 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5881 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5941 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6001 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6061 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6121 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6181 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6241 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6301 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6361 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6421 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6481 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6601 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6661 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6721 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6781 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6841 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
