LOCUS       sequence-491273-        9938 bp    DNA     circular SYN 31-OCT-2025
DEFINITION  Dual sgRNA targeting TIMM23 expressing BFP with Puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Thayer JA, Petersen JD, Huang X, Hawrot J, Ramos DM, Sekine S, Li Y,
            Ward ME, Narendra DP
  TITLE     Novel reporter of the PINK1-Parkin mitophagy pathway identifies its 
            damage sensor in the import gate.
  JOURNAL   bioRxiv [Preprint]. 2025 Feb 20:2025.02.19.639160. doi: 
            10.1101/2025.02.19.639160.
  PUBMED    40027798
REFERENCE   2  (bases 1 to 9938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 31, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Feb 20:2025.02.19.639160. doi: 
            10.1101/2025.02.19.639160."
COMMENT     Sequence Label: pLenti_DualsgRNA_TIMM23_Puro_T2A_BFP2
FEATURES             Location/Qualifiers
     source          1..9938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(41..61)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(286..306)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    310..343
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    596..713
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1011..1055)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1240..1473
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1970..2095
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2142..2322
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2340..2538)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2365..2385)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2540..2919
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3091..3110
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3185..3289
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3290..3358,3359..4150)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3508..3527)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4321..4909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4810..4829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4971..5151
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     5660..5676
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5661..5677
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5680..6268
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5786..5797
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6195..6215
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    6324..6357
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(6377..7075)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(7088..7141)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(7151..7747)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7231..7251)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7728..7747
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7759..8937)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7768..8706
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(7791..7811)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(9809..9828)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct
       61 cttcctgccc gaccttgggg cgcgccatag ctgagtgtag attcgagcaa aaaaagcacc
      121 cgactcgggt gccacttttt caagttgtaa acggactagc cttatttcaa cttgctatgc
      181 actcttgtgc ttagctctga aacctgggcc gttactcctc aaccaacaag gtggttctcc
      241 aagggatact tatagtctca aaacacacaa ttactttaca gttagggtga gtttcctttt
      301 gtgctgttta taacttcgta taatgtatgc tatacgaagt tatagaaatc caagcctatc
      361 atgtaaaatg tagctagtat taaaaagaac agattatctg tcttttatcg cacattaagc
      421 ctctatagtt actaggaaat attatatgca aattaaccgg ggcaggggag tagccgagct
      481 tctcccacaa gtccgtgcga gggggccggc gcgggcctag agatggcgcg tcggatctct
      541 agagcgggcc cggtactaac caaactggat ctctgctgtc cctgtaataa acccgaaaat
      601 tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt
      661 ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga
      721 tgaatactgc catttgtctg cagaattggc gcacgcagtg ccgatccgtt cactaatcga
      781 atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt
      841 cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta
      901 ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt
      961 atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc
     1021 catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca
     1081 attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc
     1141 gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca
     1201 gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg
     1261 tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc
     1321 aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca
     1381 gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct
     1441 gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc
     1501 accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta
     1561 tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc
     1621 agcggccggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt
     1681 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
     1741 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
     1801 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
     1861 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
     1921 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
     1981 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
     2041 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
     2101 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
     2161 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     2221 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     2281 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg caaaacgcgc
     2341 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
     2401 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
     2461 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
     2521 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     2581 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     2641 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     2701 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     2761 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     2821 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     2881 taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat ctggcccgta
     2941 catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt cttcatgcaa ttgtcggtca
     3001 agccttgcct tgttgtagct taaattttgc tcgcgcacta ctcagcgacc tccaacacac
     3061 aagcagggag cagatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     3121 accatagggg atcgggagat ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa
     3181 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3241 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3301 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3361 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3421 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3481 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3541 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3601 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3661 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3721 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3781 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3841 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3901 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3961 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4021 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4081 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4141 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4201 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4261 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4321 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4381 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4441 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4501 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4561 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4621 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4681 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4741 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4801 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4861 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4921 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
     4981 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     5041 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     5101 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
     5161 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
     5221 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
     5281 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     5341 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     5401 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     5461 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     5521 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     5581 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     5641 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg
     5701 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     5761 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     5821 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     5881 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     5941 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     6001 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     6061 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     6121 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     6181 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     6241 cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt
     6301 ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag
     6361 gtccctcgac gaattcttaa ttaagcttgt gccccagttt gctagggagg tcgcagtatc
     6421 tggccactgc cacctcgtgc tgctcgacgt aggtctcgtt gttggcctcc ttgattcttt
     6481 ccagtctgta gtccacatag tagacgccag gcatcttgag gttcttagcg ggtttcttgg
     6541 atctatatgt ggtcttgatg tttgcgatca gatggctccc gcccacgagc ttcagggcca
     6601 tgtcgtttct gccttccagg ccgccgtcag cggggtacag ggtctcggtg aaggcctccc
     6661 agccgagtgt tttcttctgc atcacagggc cgttggatgt gaagttcacc cctctgatct
     6721 tgacgttgta gatgaggcag ccgtcctgga ggctggtgtc ctgggtagcg gtcagcacgc
     6781 ccccgtcttc gtatgtggtg actctctccc atgtgaagcc ctcagggaag gactgcttga
     6841 agaagtcggg gatgccctgg gtgtggttga tgaaggtctt gctgccgtag aggaagctag
     6901 tagccaggat gtcgaaggcg aaggggagag ggccgccctc gaccaccttg attctcatgg
     6961 tctgggtgcc ctcgtagggc ttgccttcgc cctcggatgt gcacttgaag tgatggttgt
     7021 ccacggtgcc ctccatgtac agcttcatgt gcatgttctc cttaatcagc tcgctggtgg
     7081 cgaccggtgg gccaggattc tcctcgacgt caccgcatgt tagcagactt cctctgccct
     7141 ctcccgatcc ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
     7201 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
     7261 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
     7321 cggcgctgcc cagacccttg ccctggtggt cgggcgacac gccgacggtg gccaggaacc
     7381 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
     7441 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
     7501 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
     7561 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
     7621 tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
     7681 gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
     7741 cggtcatggt gggctagctc acgacacctg aaatggaaga aaaaaacttt gaaccactgt
     7801 ctgaggcttg agaatgaacc aagatccaaa ctcaaaaagg gcaaattcca aggagaatta
     7861 catcaagtgc caagctggcc taacttcagt ctccacccac tcagtgtggg gaaactccat
     7921 cgcataaaac ccctcccccc aacctaaaga cgacgtactc caaaagcgcg agaactaatc
     7981 gaggtgcctg gacggcgccc ggtactccgt ggagtcacat gaagcgacgg ctgaggacgg
     8041 aaaggccctt ttcctttgtg tgggtgactc acccgcccgc tctcccgagc gccgcgtcct
     8101 ccattttgag ctccctgcag cagggccggg aagcggccat ctttccgctc acgcaactgg
     8161 tgccgaccgg gccagccttg ccgcccaggg cggggcgata cacggcggcg cgaggccagg
     8221 caccagagca ggccggccag cttgagacta cccccgtccg attctcggtg gccgcgctcg
     8281 caggccccgc ctcgccgaac atgtgcgctg ggacgcacgg gccccgtcgc cgcccgcggc
     8341 cccaaaaacc gaaataccag tgtgcagatc ttggcccgca tttacaagac tatcttgcca
     8401 gaaaaaaagc gtcgcagcag gtcatcaaaa attttaaatg gctagagact tatcgaaagc
     8461 agcgagacag gcgcgaaggt gccaccagat tcgcacgcgg cggccccagc gcccaggcca
     8521 ggcctcaact caagcacgag gcgaaggggc tccttaagcg caaggcctcg aactctccca
     8581 cccacttcca acccgaagct cgggatcaag aatcacgtac tgcagccagg tggaagtaat
     8641 tcaaggcacg caagggccat aacccgtaaa gaggccaggc ccgcgggaac cacacacggc
     8701 acttacctgt gttctggcgg caaacccgtt gcgaaaaaga acgttcacgg cgactactgc
     8761 acttatatac ggttctcccc caccctcggg aaaaaggcgg agccagtaca cgacatcact
     8821 ttcccagttt accccgcgcc accttctcta ggcaccggtt caattgccga cccctccccc
     8881 caacttctcg gggactgtgg gcgatgtgcg ctctgcccac tgacgggcac atgcatctcc
     8941 gcgcctacag ctcaagccac atccgaaggg ggagggagcc gggagctgcg cgcggggccg
     9001 ccggggggag gggtggcacc gcccacgccg ggcggccacg aagggcgggg cagcgggcgc
     9061 gcgcccggcg gggggagggg ccgcgcgccg cgcccgctgg gaattggggc cctaggggga
     9121 gggcggaggc gccgacgacc gcggcactta ccgttcgcgg cgtggcgccc ggtggtcccc
     9181 aaggggaggg aagggggagg cggggcgagg acagtgaccg gagtctcctc agcggtggct
     9241 tttctgcttg gcagcctcag cggctggcgc caaaaccgga ctccgcccac ttcctcgccc
     9301 ctgcggtgcg agggtgtgga atcctccaga cgctggggga gggggagttg ggagcttaaa
     9361 aactagtacc cctttgggac cactttcagc agcgaactct cctgtacacc aggggtcagt
     9421 tccacagacg cgggccaggg gtgggtcatt gcggcgtgaa caataatttg actagaagtt
     9481 gattcgggtg tttccggaag gggccgagtc aatccgccga gttggggcac ggaaaacaaa
     9541 aagggaaggc tactaagatt tttctggcgg gggttatcat tggcgtaact gcagggacca
     9601 cctcccggcg gccgcctaat ggatcctagt actcgagaaa aaaagcaccg actcggtgcc
     9661 actttttcaa gttgataacg gactagcctt atttaaactt gctatgctgt ttccagctta
     9721 gctcttaaac acttcacacg ctgaccttcc catgtttctg gctttccaca agatatataa
     9781 agccaagaaa tcgaaatact ttcaagttac ggtaagcata tgatagtcca ttttaaaaca
     9841 taattttaaa actgcaaact acccaagaaa ttattacttt ctacgtcacg tattttgtac
     9901 taatatcttt gtgtttacag tcaaattaat tccaatta
//
