LOCUS       sequence-491307-       11286 bp    DNA     circular SYN 31-OCT-2025
DEFINITION  Expresses the non-responsive yEPAC-R279L sensor in budding yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Botman D, O'Toole TG, Goedhart J, Bruggeman FJ, van Heerden JH, 
            Teusink B
  TITLE     A yeast FRET biosensor enlightens cAMP signaling.
  JOURNAL   Mol Biol Cell. 2021 Jun 15;32(13):1229-1240. doi: 
            10.1091/mbc.E20-05-0319. Epub 2021 Apr 21.
  PUBMED    33881352
REFERENCE   2  (bases 1 to 11286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 31, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E20-05-0319"; journalName: "Mol Biol Cell"; date: 
            "2021-06-15- 15"; volume: "32"; issue: "13"; pages: "1229-1240"
COMMENT     Sequence Label: yEPAC-R279L pDRF1-GW
FEATURES             Location/Qualifiers
     source          1..11286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    798..822
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(833..835,836..838,839..1351)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(878..899)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1139..1158)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1486..1507
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3737..5167
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(5176..5200)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5262..5281)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5263..5281)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      5429..5616
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5484..5508)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(5628..5645)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5628..5644)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5637..5659)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6080..7426
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(7537..8340)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8341..8556)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8581..8599)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8667..8771
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8772..8840,8841..9632)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8990..9009)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9803..10391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10292..10311
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10545..10562
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10679..10700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10715..10720,10721..10738,10739..10745)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10753..10769
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10758..10780
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10777..10793
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10777..10793
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(10823..11278)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10960..10981)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11172..11191
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttacctctg tggaaattgt tactctcaca ctctttagtt cgtttgtttg
      421 ttttgtttat tccaattatg accggtgacg aaacgtggtc gatggtgggt accgcttatg
      481 ctcccctcca ttagtttcga ttatataaaa aggccaaata ttgtattatt ttcaaatgtc
      541 ctatcattat cgtctaacat ctaatttctc ttaaattttt tctctttctt tcctataaca
      601 ccaatagtga aaatcttttt ttcttctata tctacaaaaa cttttttttt ctatcaacct
      661 cgttgataaa ttttttcttt aacaatcgtt aataattaat taattggaaa ataaccattt
      721 tttctctctt ttatacacac attcaaaaga aagaaaaaaa atatacccca gcctcgacta
      781 gtgaattcgc ggccatcaca agtttgtaca aaaaagcagg ctgctagccg caatggtgag
      841 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      901 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      961 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1021 caccctgtcc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     1081 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1141 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1201 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1261 gtacaactac tttagcgaca acgtctatat caccgccgac aagcagaaga acggcatcaa
     1321 ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta
     1381 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1441 cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1501 gttcgtgacc gccgcccgat atcagcccgt gggaactcat gagatggagg aggagttggc
     1561 cgaagctgtg gccctgctct cccagcgggg gcctgacgcc ctgctcactg tggcacttcg
     1621 aaagccccca ggtcagcgca cggatgaaga gctggacctc atctttgagg agctgctgca
     1681 catcaaggct gtggcccacc tctccaactc ggtgaagcga gaattagcgg ctgttctgct
     1741 ctttgaacca cacagcaagg cagggaccgt gttgttcagc cagggggaca agggcacttc
     1801 gtggtacatt atctggaagg gatctgtcaa cgtggtgacc catggcaagg ggctggtgac
     1861 caccctgcat gagggagatg attttggaca gctggctctg gtgaatgatg cacccctggc
     1921 agccaccatc atcctgcgag aagacaactg tcatttcctg cgtgtggaca agcaggactt
     1981 caaccgtatc atcaaggatg tggaggcaaa gaccatgcgg ctggaagaac atggcaaagt
     2041 ggtgctggtg ctggagagag cctctcaggg cgccggccct tcccgacccc caaccccagg
     2101 caggaaccgg tatacagtga tgtctggcac cccagagaag atcctagagc ttctgttgga
     2161 ggccatggga ccagattcca gtgctcatga cccaacagag acattcctca gcgacttcct
     2221 cctgacccac agggtcttca tgcccagcgc ccaactctgc gctgcccttc tgcaccactt
     2281 ccatgtggag cctgcgggtg gcagcgagca ggagcgcagc acctacgtct gcaacaagag
     2341 gcagcagatc ttgcggctgg tcagccagtg ggtggccctg tatggctcca tgctccacac
     2401 tgaccctgtg gccaccagct tcctccagaa actctcagac ctggtgggca gggacacccg
     2461 actcagcaac ctgctgaggg agcagtggcc agagaggcgg cgatgccaca ggttggagaa
     2521 tggctgtggg aatgcatctc ctcagatgaa ggcccggaac ttgcctgttt ggctccccaa
     2581 ccaggacgag ccccttcctg gcagcagctg tgccatccaa gttggggata aagtccccta
     2641 tgacatctgc cggccagacc actcagtgtt gaccctgcag ctgcctgtga cagcctccgt
     2701 gagagaggtg atggcagcgt tggcccagga ggatggctgg accaaggggc aggtgctggt
     2761 gaaggtcaat tctgcaggtg atgccattgg cctgcagcca gatgcccgtg gtgtggccac
     2821 atctctgggg ctcaatgagc gtctctttgt tgtcaaccca caggaagcgc atgagctgat
     2881 cccacaccct gaccagctgg ggcccactgt gggctctgct gaggggctgg acctggtgag
     2941 tgccaaggac ctggcaggcc agctgacgga ccacgactgg agcctcttca acagtatcca
     3001 ccaggtggag ctgatccact atgtgctggg cccccagcat ctgcgggatg tcaccaccgc
     3061 caacctggag cgcttcatgc gccgcttcaa tgagctgcag tactgggtgg ccaccgagct
     3121 gtgtctctgc cccgtgcccg gcccccgggc ccagctgctc aggaagttca ttaagctggc
     3181 ggcccacctc aaggagcaga agaatctcaa ttccttcttt gccgtcatgt ttggcctcag
     3241 caactcggcc atcagccgcc tagcccacac ctgggagcgg ctgccccaca aagtccggaa
     3301 gctgtactcc gccctcgaga ggctgctgga tccctcatgg aaccaccggg tataccgact
     3361 ggccctcgcc aagctctccc ctcctgtcat ccccttcatg ccccttcttc tcaaagacat
     3421 ggccgccatt catgagggaa accacacact agtggagaat ctcatcaact ttgagaagat
     3481 gagaatgatg gccagagccg cgcggatgct gcaccactgc cgaagccaca accctgtgcc
     3541 tctctcacca ctcagaagcc gagtttccca cctccacgag gacagccagg tggcgaggat
     3601 ttccacatgc tcggagcagt ccctgagcac ccggagtcca gccagcacct gggcttatgt
     3661 ccagcagctg aaggtcattg acaaccagcg ggaactctcc cgcctctccc gagagctgga
     3721 gccagcaagc gagctcatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt
     3781 caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
     3841 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     3901 gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa
     3961 gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga
     4021 gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca
     4081 ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc
     4141 cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga
     4201 cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct
     4261 ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta
     4321 cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     4381 cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag
     4441 cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca aagagttcat
     4501 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     4561 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     4621 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     4681 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     4741 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     4801 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     4861 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     4921 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     4981 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     5041 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     5101 acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta
     5161 caagtaagcg gccgcaccca gctttcttgt acaaagtggt gtagttaatt catgatggcc
     5221 gctgcaggtc gacctcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt
     5281 acgcgcggat ccagctttgg acttcttcgc cagaggtttg gtcaagtctc caatcaaggt
     5341 tgtcggcttg tctaccttgc cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg
     5401 tagatacgtt gttgacactt ctaaataagc gaatttctta tgatttatga tttttattat
     5461 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
     5521 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
     5581 gcatgaggtc gctcttattg accacacctc taccggcatg ccaattcact ggccgtcgtt
     5641 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5701 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5761 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     5821 ggtatttcac accgcataat cggatcgtac ttgttaccca tcattgaatt ttgaacatcc
     5881 gaacctggga gttttccctg aaacagatag tatatttgaa cctgtataat aatatatagt
     5941 ctagcgcttt acggaagaca atgtatgtat ttcggttcct ggagaaacta ttgcatctat
     6001 tgcataggta atcttgcacg tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact
     6061 tcaatagcat atctttgtta acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa
     6121 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     6181 caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa
     6241 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     6301 aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac
     6361 aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc
     6421 ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt
     6481 tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc
     6541 ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc
     6601 gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg
     6661 atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta
     6721 cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac
     6781 tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag
     6841 tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca
     6901 gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt
     6961 ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc
     7021 ttttggtttt caaaagcgct ctgaagttcc tatactttct agctagagaa taggaacttc
     7081 ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg
     7141 cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata
     7201 tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat
     7261 ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt
     7321 atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg
     7381 gattagtctc atccttcaat gctatcattt cctttgatat tggatcgatc cgatgataag
     7441 ctgtcaaaca tgagaattgg gtaataactg atataattaa attgaagctc taatttgtga
     7501 gtttagtata catgcattta cttataatac agttttttag ttttgctggc cgcatcttct
     7561 caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc
     7621 ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc
     7681 cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt
     7741 cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc
     7801 gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt
     7861 agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt
     7921 tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc
     7981 attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac
     8041 tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga
     8101 taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg
     8161 tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt
     8221 ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag
     8281 cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat
     8341 gatttatctt cgtttcctgc atgtttttgt tctgtgcagt tgggttaaga atactgggca
     8401 atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt
     8461 ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa
     8521 tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagc taattcttga agacgaaagg
     8581 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     8641 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8701 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8761 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8821 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8881 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8941 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9001 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9061 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     9121 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     9181 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9241 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     9301 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     9361 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     9421 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     9481 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     9541 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     9601 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     9661 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     9721 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9781 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9841 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9901 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9961 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10021 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10081 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    10141 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10201 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    10261 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    10321 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    10381 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    10441 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    10501 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    10561 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    10621 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    10681 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    10741 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    10801 acgccaagct taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
    10861 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
    10921 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
    10981 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    11041 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
    11101 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    11161 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
    11221 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
    11281 attcgc
//
