LOCUS       sequence-491367-        7092 bp    DNA     circular SYN 31-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7092)
  TITLE     Pseudotyping Envelopes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 31, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-Eco
FEATURES             Location/Qualifiers
     source          1..7092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..662
                     /label=chicken beta-actin promoter
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     misc_feature    1666..2040
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1978..1994
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(4113..4132)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4178..4233
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4232..4251)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4594..4610)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4594..4610)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4607..4629)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4618..4634
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4642..4648,4649..4666,4667..4672))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4687..4708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4767..4962
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4813..4948
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4968..5102
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5005..5024)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5059..5078
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5170..5187)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5341..5929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5421..5440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6100..6891,6892..6960))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6723..6742
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6961..7065)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctacgcaa cacgccgggc ccccatggcc tcaccccata tgagatctta
     1681 tatggggcac ccccgcccct tgtaaacttc cctgaccctg acatgacaag agttactaac
     1741 agcccctctc tccaagctca cttacaggct ctctacttag tccagcacga agtctggaga
     1801 cctctggcgg cagcctacca agaacaactg gaccgaccgg tggtacctca cccttaccga
     1861 gtcggcgaca cagtgtgggt ccgccgacac cagactaaga acctagaacc tcgctggaaa
     1921 ggaccttaca cagtcctgct gaccaccccc accgccctca aagtagacgg catcgcagct
     1981 tggatacacg ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc ctctagactg
     2041 acatggcgcg ttcaacgctc tcaaaacccc ttaaaaataa ggttaacccg cgaggccccc
     2101 taatcccctt aattcttctg atgctcagag gggtcagtac tgcttcgccc ggctccagtc
     2161 ctcatcaagt ctataatatc acctgggagg taaccaatgg agatcgggag acggtatggg
     2221 caacttctgg caaccaccct ctgtggacct ggtggcctga ccttacccca gatttatgta
     2281 tgttagccca ccatggacca tcttattggg ggctagaata tcaatcccct ttttcttctc
     2341 ccccggggcc cccttgttgc tcagggggca gcagcccagg ctgttccaga gactgcgaag
     2401 aacctttaac ctccctcacc cctcggtgca acactgcctg gaacagactc aagctagacc
     2461 agacaactca taaatcaaat gagggatttt atgtttgccc cgggccccac cgcccccgag
     2521 aatccaagtc atgtgggggt ccagactcct tctactgtgc ctattggggc tgtgagacaa
     2581 ccggtagagc ttactggaag ccctcctcat catgggattt catcacagta aacaacaatc
     2641 tcacctctga ccaggctgtc caggtatgca aagataataa gtggtgcaac cccttagtta
     2701 ttcggtttac agacgccggg agacgggtta cttcctggac cacaggacat tactggggct
     2761 tacgtttgta tgtctccgga caagatccag ggcttacatt tgggatccga ctcagatacc
     2821 aaaatctagg accccgcgtc ccaatagggc caaaccccgt tctggcagac caacagccac
     2881 tctccaagcc caaacctgtt aagtcgcctt cagtcaccaa accacccagt gggactcctc
     2941 tctcccctac ccaacttcca ccggcgggaa cggaaaatag gctgctaaac ttagtagacg
     3001 gagcctacca agccctcaac ctcaccagtc ctgacaaaac ccaagagtgc tggttgtgtc
     3061 tagtagcggg acccccctac tacgaagggg ttgccgtcct gggtacctac tccaaccata
     3121 cctctgctcc agccaactgc tccgtggcct cccaacacaa gttgaccctg tccgaagtga
     3181 ccggacaggg actctgcata ggagcagttc ccaaaacaca tcaggcccta tgtaatacca
     3241 cccagacaag cagtcgaggg tcctattatc tagttgcccc tacaggtacc atgtgggctt
     3301 gtagtaccgg gcttactcca tgcatctcca ccaccatact gaaccttacc actgattatt
     3361 gtgttcttgt cgaactctgg ccaagagtca cctatcattc ccccagctat gtttacggcc
     3421 tgtttgagag atccaaccga cacaaaagag aaccggtgtc gttaaccctg gccctattat
     3481 tgggtggact aaccatgggg ggaattgccg ctggaatagg aacagggact actgctctaa
     3541 tggccactca gcaattccag cagctccaag ccgcagtaca ggatgatctc agggaggttg
     3601 aaaaatcaat ctctaaccta gaaaagtctc tcacttccct gtctgaagtt gtcctacaga
     3661 atcgaagggg cctagacttg ttatttctaa aagaaggagg gctgtgtgct gctctaaaag
     3721 aagaatgttg cttctatgcg gaccacacag gactagtgag agacagcatg gccaaattga
     3781 gagagaggct taatcagaga cagaaactgt ttgagtcaac tcaaggatgg tttgagggac
     3841 tgtttaacag atccccttgg tttaccacct tgatatctac cattatggga cccctcattg
     3901 tactcctaat gattttgctc ttcggaccct gcattcttaa tcgattagtc caatttgtta
     3961 aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc
     4021 ctatagagta cgagccatag ttctgagcgc cggtcgctac cattaccagt tggtctggtg
     4081 tcaaaaataa taataaccgg gcaaacggca gatctttttc cctctgccaa aaattatggg
     4141 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     4201 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     4261 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
     4321 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
     4381 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
     4441 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
     4501 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
     4561 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     4621 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4681 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4741 gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc
     4801 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4861 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     4921 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg
     4981 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5041 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     5101 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5161 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5221 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5281 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5341 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5401 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5461 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5521 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5581 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5641 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5701 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5761 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5821 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5881 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5941 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6001 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6061 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6121 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6181 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6241 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6301 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6361 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6421 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6481 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6541 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6601 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6661 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6721 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6781 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6841 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6901 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6961 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7021 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7081 agtgccacct gg
//
