LOCUS       sequence-491402-        7013 bp    DNA     circular SYN 03-NOV-2025
DEFINITION  5' AAVLINK plasmid for SETBP1 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Zhonghua Lu Lab
  TITLE     AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery 
            in Gene Therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVLINK-EF1alpha-HA-5'-SETBP1
FEATURES             Location/Qualifiers
     source          1..7013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     repeat_region   4276..4416
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4276..4405
                     /label=AAV2 ITR
     rep_origin      4491..4946
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4578..4597)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4788..4809
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4963..4982)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5082..5104
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5142..5160)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5228..5332
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5333..5401,5402..6193)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5551..5570)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6364..6952
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6853..6872
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctga gtccagggaa accttaagca gctcccggca
      781 aagagggggc gagtcagact tcctgccggt ctcctcagcc aagcccccag ctgctcctgg
      841 ctgtgcagga gaacctttgc tctccactcc aggacctggg aaggggatcc cggtgggcgg
      901 agagcgcatg gagccagagg aggaggatga actaggctca gggcgggatg tggattccaa
      961 ctccaacgcg gacagtgaga aatgggtggc aggagatggt ttggaagagc aggaattttc
     1021 tatcaaggag gcaaacttca cagagggaag tctgaagcta aagattcaga ccacaaagcg
     1081 ggctaagaaa cccccaaaga atttggagaa ctatatatgt ccacctgaga tcaagatcac
     1141 catcaagcag tctggggacc agaaggtgtc ccgtgctgga aaaaatagca aagccacgaa
     1201 ggaggaagaa agaagccact ccaaaaagaa gctcctcaca gccagtgacc ttgcagccag
     1261 tgacctcaaa ggatttcagc cacaggctta cgagaggccc cagaaacatt caactctcca
     1321 ttatgacacg ggcctcccac aggacttcac cggtgacacc ttaaaaccaa agcaccagca
     1381 aaaaagcagc agccagaacc acatggactg gtccaccaac tctgacagcg gacccgtcac
     1441 tcagaattgc ttcatcagtc cagagtctgg cagagaaact gcaagcacca gcaagatccc
     1501 cgctcttgag cccgtggctt cctttgcaaa ggcccagggt aagaaaggca gtgcagggaa
     1561 cacgtggagt cagttgtcta acaataacaa agatctgctc ttgggaggtg tggctccatc
     1621 cccaagcagc cacagctcac cagccccacc cagcagctct gctgagtgca acgggcttca
     1681 gcccttggtg gatcaagatg gaggaggtac aaaggagccc ccagaaccac ctacggtggg
     1741 cagcaagaaa aagtccagta aaaaagatgt gataagtcag accataccaa acccagacct
     1801 ggattgggtc aagaatgccc agaaagcatt tgacaataca gaagggaaaa gggaaggtta
     1861 ttccgcagat agtgcccaag aggcatcacc agccaggcag aacgtgagtt ctgccagtaa
     1921 tcctgaaaat gactcaagtc atgtccggat tactatcccc atcaaggcac cctctctgga
     1981 tccaaccaac cataagagga aaaaaagaca gtccattaaa gcggtggtgg aaaagatcat
     2041 gccagagaaa gccttggctt ctggaatcac catgagcagt gaagtagtta acaggatact
     2101 ttccaactct gaggggaata agaaggatcc ccgtgtccct aagttgagta aaatgataga
     2161 gaatgagtcc ccctcagttg gccttgaaac tggtggaaat gctgagaaag ttatcccagg
     2221 aggtgtgtct aagccgcgga agccacccat ggtcatgaca cctccaacgt gcacagatca
     2281 ctctccatcc agaaagctgc cagaaatcca gcatccaaaa tttgctgcaa aacgaaggtg
     2341 gacttgcagc aaaccaaaac ctagcaccat gcttcgagag gcagttatgg ccacctctga
     2401 taaactgatg ctggagcccc cgtctgcata tcccatcacc acatccagcc ctctctacac
     2461 caacacagac agtcttactg tgatcactcc agtcaaaaag aagcggggac gaccaaagaa
     2521 gcagcctttg cttacagtcg agacgattca tgagggaact tccaccagcc ccgtcagtcc
     2581 catcagccga gagtttcctg gcactaagaa aagaaagcga cgacgcaatt tagcgaagtt
     2641 ggcccagcta gtgccgggag aggacaaacc catgagcgag atgaaatttc acaagaaagt
     2701 tggaaagctc ggcgtgttgg ataagaagac catcaaaact atcaataaga tgaagacact
     2761 caagaggaaa aacatcttga atcagatctt gtcctgttcc agcagcgttg ctctgaaggc
     2821 aaaagctccc ccagagacca gccctggggc agcagccatt gaaagcaaac tgggcaagca
     2881 gattaatgtc agcaagaggg gaaccatcta cattggcaag aagcggggca ggaagccaag
     2941 agcagagctg ccacccccat ccgaagaacc caaaacagcc atcaagcacc ccaggcctgt
     3001 ttctagccag ccggatgttc cagccgtgcc ttccaacttt cagtcacttg tggcgtcttc
     3061 accagcagct atgcacccac tttcaacaca gttaggtggg tccaatggca acctgagccc
     3121 tgccagcact gaaaccaatt tttcagagtt gaaaactatg ccaaatctcc agcccatcag
     3181 tgctcttcca accaaaaccc aaaagggaat acacagtgga acctggaagc tgtctccacc
     3241 cagactgatg gccaactccc cttcacacct gtgcgagatt ggctccctaa aggaaatcac
     3301 gctgtcccct gtgagcgagt cccacagtga ggagacgatc cccagcgaca gcggcattgg
     3361 gacagacaac aacagcactt ctgaccaagc ggagaagagc tcagaatccc gaaggaggta
     3421 ctcttttgat ttctgctccc tggacaaccc ggaggccatt ccgtccgaca ccagcacaaa
     3481 gaaccggcat ggccaccggc aaaagcatct cattgtggac aactttctgg cccacgaaag
     3541 cctcaagaag ccaaagcaca agaggaaacg gaaaagcctg caaaaccgcg atgacctcca
     3601 gtttctggca gacctggagg agctaatcac caagttccaa gtgttcagaa tctcccaccg
     3661 gagttacacc ttctaccacg agaatccata tcccagcatt tttcggatta attttgatca
     3721 ctattacccg gtgccatata tccagtatga cccgttgctc tatcttcgta ggacttcaga
     3781 cttgaagtca aagaagaagc gtggtaggcc tgcaaaaacc aatgacacca tgacaaaggt
     3841 gcctttttta caagggttca gctaccctat tcccagtgga agttactatg caccctatgg
     3901 aatgccttac acatcaatgc ctatgatgaa ccttggttat tacggtcagt acccagctcc
     3961 tttgtaccta tcgcacacgc ttggagcagc ttccccattc atgaggccaa cagtgccacc
     4021 acctcagttc cacacaaact cccacgtaaa gatgtccggt gcagctaagc ataaagccaa
     4081 gcatggagta cacctgcagg gacctgttag catgggcctt ggtgacatgc agccttctct
     4141 gaaccctccc aaggtaagta tcaaggttac aagacaggtt taaggagacc aataagctat
     4201 tattcgtata ggatacttta tacgaagtta ttaagttatg gagaccggtg tggcacgtgc
     4261 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     4321 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     4381 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     4441 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     4501 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4561 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4621 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4681 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     4741 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4801 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
     4861 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4921 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     4981 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     5041 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     5101 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     5161 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5221 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5281 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5341 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5401 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5461 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5521 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5581 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5641 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5701 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5761 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5821 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5881 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5941 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6001 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6061 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6121 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6181 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6241 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6301 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6361 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6421 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6481 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6541 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6601 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6661 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6721 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6781 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6841 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6901 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6961 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
