LOCUS       sequence-491425-        9434 bp    DNA     circular SYN 03-NOV-2025
DEFINITION  Encodes PhiC31 recombinase driven by the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Schick E, McCaffery SD, Keblish EE, Thakurdin C, Emerson MM
  TITLE     Lineage tracing analysis of cone photoreceptor associated 
            cis-regulatory elements in the developing chicken retina.
  JOURNAL   Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.1038/s41598-019-45750-7.
  PUBMED    31249345
REFERENCE   2  (bases 1 to 9434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-06-27"; volume: "9(1):9358. doi"; pages: " 
            10.1038/s41598-019-45750-7"
COMMENT     Sequence Label: CAG::PhiC31
FEATURES             Location/Qualifiers
     source          1..9434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    47..95
                     /note="synthetic polyadenylation signal"
     misc_feature    109..200
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     149..168
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     enhancer        211..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        592..867
                     /label=chicken beta-actin promoter
     intron          868..1884
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1808..1830
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1892..1911
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2019..3833
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             3834..3854
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3898..4450
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4042..4059)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4269..4288
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4462..5961
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     primer_bind     complement(6456..6475)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6521..6576
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6575..6594)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6937..6953)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6937..6953)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6950..6972)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6961..6977
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6985..6991,6992..7009,7010..7015))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7030..7051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7110..7305
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7156..7291
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7311..7445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7348..7367)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7402..7421
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7513..7530)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7684..8272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7764..7783)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8443..9234,9235..9303))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9066..9085
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9304..9408)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgagaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
       61 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      121 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      181 aggtgccaga acatttctct atcgatagtc gacattgatt attgactagt tattaatagt
      241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
      601 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
      661 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gsgcgcgcca
      721 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
      781 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
      841 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
      901 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
      961 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
     1021 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
     1081 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
     1141 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     1201 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
     1261 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
     1321 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
     1381 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
     1441 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
     1501 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
     1561 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
     1621 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
     1681 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
     1741 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
     1801 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
     1861 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
     1921 cattttggca aagaattcta tataatggaa gcttgaccgg agccgttagt tgcccgtcgg
     1981 acgcgatttg cgaggcgctt tatgcgcgca ccgggaccat ggatacctac gccggagcct
     2041 acgacagaca gagccgggag agagagaaca gcagcgccgc cagccccgcc acccagagaa
     2101 gcgccaacga ggataaggcc gccgatctgc agagagaggt ggagagggac ggcggcagat
     2161 tcagatttgt gggccacttc agcgaggccc ctggcaccag cgccttcggc accgccgaga
     2221 gacccgagtt cgagagaatc ctgaacgagt gtagggccgg caggctgaac atgatcatcg
     2281 tgtacgacgt gtcccggttc agcaggctga aggtgatgga cgccatccct atcgtgtccg
     2341 agctgctggc cctgggcgtg accatcgtgt ccacccagga aggcgtcttt agacagggca
     2401 acgtgatgga cctgatccac ctgatcatga ggctggacgc cagccacaag gagagcagcc
     2461 tgaagagcgc caagatcctg gacaccaaga acctgcagag ggagctgggc ggctatgtgg
     2521 gcggcaaggc cccctacggc ttcgagctgg tgtccgagac caaggagatc acccggaacg
     2581 gcaggatggt gaacgtggtg atcaacaagc tggcccacag caccaccccc ctgaccggcc
     2641 ccttcgagtt tgagcccgac gtgatcaggt ggtggtggcg ggagatcaag acccacaagc
     2701 acctgccttt caagcccggc agccaggccg ccatccaccc cggcagcatc accggcctgt
     2761 gtaagagaat ggacgccgac gccgtgccca ccagaggcga gaccatcggc aagaaaaccg
     2821 ccagcagcgc ctgggacccc gccaccgtga tgagaatcct gagggaccct aggatcgccg
     2881 gcttcgccgc cgaggtgatc tacaagaaga agcccgacgg cacccccacc accaagatcg
     2941 agggctacag aatccagaga gaccccatca ccctgagacc tgtggagctg gactgtggcc
     3001 ctatcatcga gcctgccgag tggtacgagc tgcaggcctg gctggacggc agaggcagag
     3061 gcaagggcct gagcagaggc caggccatcc tgagcgccat ggacaagctg tactgtgagt
     3121 gtggcgccgt gatgaccagc aagagaggcg aggagagcat caaggacagc taccggtgcc
     3181 ggagaagaaa ggtggtggac cccagcgccc ctggccagca cgagggcacc tgtaatgtga
     3241 gcatggccgc cctggacaag ttcgtggccg agcggatctt caacaagatc cggcacgccg
     3301 agggcgacga ggagaccctg gccctgctgt gggaggccgc cagaagattc ggcaagctga
     3361 ccgaggcccc cgagaagagc ggcgagaggg ccaacctggt ggccgagaga gccgacgccc
     3421 tgaacgccct ggaggagctg tacgaggaca gagccgccgg agcctatgac ggccctgtgg
     3481 gcaggaagca cttcagaaag cagcaggccg ccctgaccct gagacagcag ggcgccgagg
     3541 aaagactggc cgagctggag gccgccgagg cccctaagct gcccctggat cagtggttcc
     3601 ccgaggatgc cgacgccgac cccaccggcc ccaagtcctg gtggggcaga gccagcgtgg
     3661 acgacaagag ggtgttcgtg ggcctgttcg tggataagat cgtggtgacc aagagcacca
     3721 ccggcagggg ccagggcacc cccatcgaga agagagccag catcacctgg gccaagcctc
     3781 ccaccgacga cgacgaggat gacgcccagg acggcaccga ggacgtggcc gcccctaaga
     3841 aaaagcggaa agtgtgatgt acaagtaaag cggccaattc csccyccccc ccccctaacg
     3901 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     3961 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     4021 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     4081 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     4141 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     4201 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     4261 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     4321 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     4381 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     4441 gatgataata tggggtcgag gctgctgctg ctgctgctgc tgggcctgag gctacagctc
     4501 tccctgggca tcatcccagt tgaggaggag aacccggact tctggaaccg cgaggcagcc
     4561 gaggccctgg gtgccgccaa gaagctgcag cctgcacaga cagccgccaa gaacctcatc
     4621 atcttcctgg gcgatgggat gggggtgtct acggtgacag ctgccaggat cctaaaaggg
     4681 cagaagaagg acaaactggg gcctgagata cccctggcca tggaccgctt cccatatgtg
     4741 gctctgtcca agacatacaa tgtagacaaa catgtgccag acagtggagc cacagccacg
     4801 gcctacctgt gcggggtcaa gggcaacttc cagaccattg gcttgagtgc agccgcccgc
     4861 tttaaccagt gcaacacgac acgcggcaac gaggtcatct ccgtgatgaa tcgggccaag
     4921 aaagcaggga agtcagtggg agtggtaacc accacacgag tgcagcacgc ctcgccagcc
     4981 ggcacctacg cccacacggt gaaccgcaac tggtactcgg acgccgacgt gcctgcctcg
     5041 gcccgccagg aggggtgcca ggacatcgct acgcagctca tctccaacat ggacattgac
     5101 gtgatcctag gtggaggccg aaagtacatg tttcgcatgg gaaccccaga ccctgagtac
     5161 ccagatgact acagccaagg tgggaccagg ctggacggga agaatctggt gcaggaatgg
     5221 ctggcgaagc gccagggtgc ccggtatgtg tggaaccgca ctgagctcat gcaggcttcc
     5281 ctggacccgt ctgtgaccca tctcatgggt ctctttgagc ctggagacat gaaatacgag
     5341 atccaccgag actccacact ggacccctcc ctgatggaga tgacagaggc tgccctgcgc
     5401 ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg agggtggtcg catcgaccat
     5461 ggtcatcatg aaagcagggc ttaccgggca ctgactgaga cgatcatgtt cgacgacgcc
     5521 attgagaggg cgggccagct caccagcgag gaggacacgc tgagcctcgt cactgccgac
     5581 cactcccacg tcttctcctt cggaggctac cccctgcgag ggagctccat cttcgggctg
     5641 gcccctggca aggcccggga caggaaggcc tacacggtcc tcctatacgg aaacggtcca
     5701 ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg agagcgagag cgggagcccc
     5761 gagtatcggc agcagtcagc agtgcccctg gacgaagaga cccacgcagg cgaggacgtg
     5821 gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg gcgtgcagga gcagaccttc
     5881 atagcgcacg tcatggcctt cgccgcctgc ctggagccct acaccgcctg cgacctggcg
     5941 ccccccgccg gcaccaccga cgccgcgcac ccggggcggt ccgtggtccc cgcgttgctt
     6001 cctctgctgg ccgggaccct gctgctgctg gagacggcca ctgctccctg agtgtcccgt
     6061 ccctggggct cctgcttccc catcccggag ttctcctgct ccccacctcc tgtcgtcctg
     6121 cctggcctcc agcccgagtc gtcatccccg gagtccctat acagaggtcc tgccatggaa
     6181 ccttcccctc cccgtgcgct ctggggactg agcccatgac accaaacctg ccccttggct
     6241 gctctcggac tccctacccc aaccccaggg actgcaggtt gtgccctgtg gctgcctgca
     6301 ccccaggaaa ggagggggct caggccatcc agccaccacc tacagcccag tggggtcgag
     6361 gatatcgcgg ccggccgcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     6421 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     6481 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     6541 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     6601 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     6661 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     6721 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     6781 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     6841 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     6901 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6961 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7021 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7081 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     7141 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7201 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7261 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
     7321 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7381 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7441 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7501 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7561 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7621 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7681 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7741 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7801 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7861 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7921 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7981 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8041 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8101 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8161 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8221 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8281 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8341 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8401 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8461 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8521 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8581 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8641 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8701 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8761 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8821 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8881 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8941 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9001 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9061 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9121 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9181 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9241 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9301 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9361 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9421 aaaagtgcca cctg
//
