LOCUS       sequence-491508-       10216 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  Expresses an NGI-resistant version of STT3A, STT3A F256P, in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Lampson BL, Ramiotarez AS, Baro M, He L, Hegde M, Koduri V, Pfaff 
            JL, Hanna RE, Kowal J, Shirole NH, He Y, Doench JG, Contessa JN, 
            Locher KP, Kaelin WG Jr
  TITLE     Positive selection CRISPR screens reveal a druggable pocket in an 
            oligosaccharyltransferase required for inflammatory signaling to 
            NF-kappaB.
  JOURNAL   Cell. 2024 Apr 25;187(9):2209-2223.e16. doi: 
            10.1016/j.cell.2024.03.022.
  PUBMED    38670073
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.03"; journalName: "Cell"; date: "2024-04-25- 
            25"; volume: "187"; issue: "9"; pages: "2209-2223.e16"
COMMENT     Sequence Label: STT3A F256P
FEATURES             Location/Qualifiers
     source          1..10216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             17..650
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    697..822
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1319..1552
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1737..1781
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2044..2159
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2217..2520
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2521..2724
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2675..2695
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2721..2745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        5067..5566
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5102..5125)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5499..5518
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5587..6186
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5587..5606)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6083..6103
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6200..6788
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6253..6273)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6671..6682)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6995..7628
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7757..7773)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7757..7773)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7770..7792)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7781..7797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7805..7811,7812..7829,7830..7835))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7850..7871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7988..8005)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8159..8747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8239..8258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8918..9709,9710..9778))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9541..9560
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9779..9883)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9931..10065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9968..9987)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10022..10041
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
       61 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
      121 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
      181 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
      241 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
      301 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
      361 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
      421 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
      481 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      541 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      601 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      661 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      721 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      781 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      841 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      901 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      961 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1021 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1081 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1141 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     1201 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1261 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1321 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1381 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1441 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1501 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1561 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1621 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1681 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1741 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1801 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1861 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1921 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     1981 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     2041 gcctttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2101 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     2161 cgggtttatt acagggacag cagagatcca gtttatcgat aagcttggga gttccgcgtt
     2221 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     2281 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2341 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2401 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2461 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2521 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2581 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2641 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2701 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2761 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcgc tagcgctacc
     2821 ggactcagat ctcgagctca tcgggatccg ccaccatgac taagtttgga tttttgcgat
     2881 tgtcctatga gaagcaggac acacttttga agcttctcat tctgtcaatg gctgctgtat
     2941 tatccttctc cactcgtctg tttgctgtcc tgagatttga aagtgttatc catgagtttg
     3001 atccgtactt taattatcgg actaccaggt tcctggctga ggaggggttt tataaattcc
     3061 ataactggtt tgatgaccga gcctggtacc ctttgggacg aatcattgga ggaacaattt
     3121 acccaggttt aatgatcacc tctgctgcaa tctaccatgt actccatttt ttccacatca
     3181 ccatcgacat tcggaatgtc tgtgtgttcc tggcccctct cttctcctcc ttcacaacga
     3241 tcgtcacgta ccaccttacc aaagagctca aggatgcagg ggctgggctt cttgctgctg
     3301 ccatgattgc tgtagttcct ggatatatct cccgatctgt ggctggctcc tatgataatg
     3361 aagggattgc catcttttgc atgctactca cctactacat gtggatcaag gcagtaaaga
     3421 ctggttccat ctgttgggca gctaagtgtg cccttgctta tttctacatg gtctcgtcat
     3481 ggggaggtta tgtgttcctg atcaacttaa ttcctctcca cgtcctcgtg ctgatgctca
     3541 caggccgttt ctctcaccgg atctatgtgg cctactgtac tgtttactgc ctgggcacta
     3601 tactttctat gcagatctcc cctgtgggtt tccagcctgt cctttcatca gagcacatgg
     3661 cagcctttgg ggtctttggt ctctgccaga tccatgcctt tgtggattac ctgcgcagca
     3721 agttgaatcc acaacaattt gaagttcttt tccggagcgt catctctctg gtaggctttg
     3781 tccttctcac cgtgggagct ctcctcatgc tgacaggaaa aatatctccc tggacggggc
     3841 gtttctactc gctgctagat ccctcttatg ctaagaacaa catccccatc attgcttctg
     3901 tgtctgagca tcagcccaca acctggtcct catactattt tgacctgcag ctactcgtct
     3961 tcatgtttcc agttggtctg tattactgct ttagcaacct gtctgatgcc cggattttta
     4021 tcatcatgta tggtgtgacc agcatgtact tttcagctgt aatggtgcgt ctaatgctag
     4081 tgttggcacc tgttatgtgc attctctctg gcattggagt ctcccaggtg ctgtccacat
     4141 acatgaagaa tctggacata agtcgtccag acaagaagag caagaagcaa caggattcca
     4201 cctaccctat taagaatgaa gtggcaagtg ggatgatact ggtcatggct ttctttctca
     4261 tcacctacac ctttcattca acctgggtga ccagtgaggc ctactcttct ccgtccattg
     4321 tactatctgc ccgtggtggg gatggcagta ggatcatatt tgatgacttc cgagaagcat
     4381 attattggct tcgtcataat actccagagg atgcgaaggt catgtcctgg tgggattatg
     4441 gctatcagat tacagctatg gcaaaccgaa caattttagt ggacaataac acatggaata
     4501 atacccatat ttctcgagta gggcaggcaa tggcgtccac agaggaaaaa gcctatgaga
     4561 tcatgaggga gctcgatgtc agctatgtgc tggtcatttt tggaggcctc actgggtatt
     4621 cctctgatga tatcaacaag tttctttgga tggtccggat tggagggagc acagatacag
     4681 gcaaacatat caaggagaat gactattata ctccaactgg ggagttccgt gtggaccgtg
     4741 aaggttctcc agtgctgctc aactgcctca tgtacaagat gtgttactat cgctttggac
     4801 aggtttacac agaagccaag cgtcctccag gctttgaccg tgtccgaaat gctgagattg
     4861 ggaataaaga ctttgagctt gatgtcctgg aggaagcata taccacagaa cattggctgg
     4921 tcaggatata caaggtaaag gacctggata atcgaggctt gtcaaggaca ggctcgggca
     4981 gcggtggctc tggaggttct gtgagcggct ggcggctgtt caagaagatt agctaagaat
     5041 tccgacatgc tctagataat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     5101 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     5161 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     5221 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     5281 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     5341 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     5401 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     5461 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     5521 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag
     5581 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     5641 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     5701 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5761 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     5821 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     5881 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     5941 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6001 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6061 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6121 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6181 gcctgaccgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6241 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6301 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6361 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6421 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6481 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6541 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     6601 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6661 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6721 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6781 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt
     6841 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     6901 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     6961 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga
     7021 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     7081 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     7141 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     7201 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     7261 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     7321 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     7381 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     7441 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7501 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7561 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7621 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7681 aatatcagag agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga
     7741 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7801 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7861 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7921 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7981 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8041 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8101 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8161 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8221 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8281 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8341 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8401 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8461 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8521 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8581 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8641 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8701 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8761 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8821 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8881 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9001 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9061 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9301 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9361 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9901 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
     9961 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    10021 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    10081 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    10141 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    10201 atttgttaaa tatgta
//
