LOCUS       sequence-491509-        8815 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  Lentiviral-based expression of EGFP with an ER retention signal 
            sequence and Q185N N186C, an STT3B-specific glcosylation sequon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Lampson BL, Ramiotarez AS, Baro M, He L, Hegde M, Koduri V, Pfaff 
            JL, Hanna RE, Kowal J, Shirole NH, He Y, Doench JG, Contessa JN, 
            Locher KP, Kaelin WG Jr
  TITLE     Positive selection CRISPR screens reveal a druggable pocket in an 
            oligosaccharyltransferase required for inflammatory signaling to 
            NF-kappaB.
  JOURNAL   Cell. 2024 Apr 25;187(9):2209-2223.e16. doi: 
            10.1016/j.cell.2024.03.022.
  PUBMED    38670073
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.03"; journalName: "Cell"; date: "2024-04-25- 
            25"; volume: "187"; issue: "9"; pages: "2209-2223.e16"
COMMENT     Sequence Label: ER-GFP Q185N N186C pLVX CMVpuro
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..531
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(67..90)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     464..483
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             552..1151
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(552..571)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1048..1068
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1165..1753
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1218..1238)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1636..1647)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1960..2593
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2722..2738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2722..2738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2735..2757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2746..2762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2770..2776,2777..2794,2795..2800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2815..2836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2953..2970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3124..3712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3204..3223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3883..4674,4675..4743))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4506..4525
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4744..4848)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4896..5030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4933..4952)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4987..5006
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5198..5831
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5878..6003
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6500..6733
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6918..6962
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7225..7340
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        7398..7701
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7702..7905
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7856..7876
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7902..7926
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(8088..8090,8091..8801)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQNCTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8130..8151)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8391..8410)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8738..8759
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agaattccga catgctctag ataattctac cgggtagggg aggcgctttt cccaaggcag
       61 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
      121 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
      181 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
      241 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
      301 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
      361 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
      421 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
      481 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
      541 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
      601 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
      661 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
      721 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
      781 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
      841 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
      901 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
      961 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     1021 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     1081 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     1141 ccggtgcctg accgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     1201 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     1261 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     1321 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     1381 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     1441 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     1501 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     1561 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     1621 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     1681 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     1741 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     1801 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     1861 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     1921 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     1981 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     2041 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     2101 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     2161 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     2221 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     2281 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     2341 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     2401 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     2461 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     2521 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     2581 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     2641 aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag
     2701 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2761 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2821 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2881 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2941 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3001 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3061 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3121 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3181 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3241 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3301 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3361 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3421 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3481 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3541 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3601 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3661 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3721 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3781 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3841 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3901 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3961 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4021 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4081 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4141 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4201 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4261 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4321 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4381 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4441 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4501 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4561 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4621 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4681 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4741 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4801 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4861 aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg
     4921 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4981 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
     5041 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
     5101 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
     5161 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
     5221 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
     5281 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     5341 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
     5401 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     5461 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
     5521 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     5581 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
     5641 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5701 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5761 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     5821 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     5881 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     5941 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     6001 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     6061 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     6121 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     6181 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     6241 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     6301 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
     6361 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     6421 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     6481 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     6541 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     6601 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     6661 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     6721 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     6781 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     6841 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     6901 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     6961 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     7021 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     7081 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     7141 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     7201 tgaacggatc tcgacggtat cgcctttaaa agaaaagggg ggattggggg gtacagtgca
     7261 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     7321 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttatcga
     7381 taagcttggg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     7441 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     7501 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     7561 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     7621 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     7681 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     7741 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     7801 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     7861 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     7921 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctac
     7981 tagaggatcg ctagcgctac cggactcaga tctcgagctc atcgggatcc gccaccatgc
     8041 tgctatccgt gccgctgctg ctcggcctcc tcggcctggc cgtcgccgtg agcaagggcg
     8101 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     8161 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     8221 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     8281 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     8341 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     8401 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     8461 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     8521 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     8581 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagaatt
     8641 gcacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     8701 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     8761 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaaggatgaa cttta
//
