LOCUS       sequence-491510-       10216 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  Expresses STT3A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Lampson BL, Ramiotarez AS, Baro M, He L, Hegde M, Koduri V, Pfaff 
            JL, Hanna RE, Kowal J, Shirole NH, He Y, Doench JG, Contessa JN, 
            Locher KP, Kaelin WG Jr
  TITLE     Positive selection CRISPR screens reveal a druggable pocket in an 
            oligosaccharyltransferase required for inflammatory signaling to 
            NF-kappaB.
  JOURNAL   Cell. 2024 Apr 25;187(9):2209-2223.e16. doi: 
            10.1016/j.cell.2024.03.022.
  PUBMED    38670073
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.03"; journalName: "Cell"; date: "2024-04-25- 
            25"; volume: "187"; issue: "9"; pages: "2209-2223.e16"
COMMENT     Sequence Label: STT3A
FEATURES             Location/Qualifiers
     source          1..10216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             86..719
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    926..1514
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1032..1043
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1441..1461
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1528..2127)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1611..1631)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2108..2127
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2148..2647)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2196..2215)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     2589..2612
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(4969..4993)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4990..5193)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5019..5039)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5194..5497
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    5555..5670
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(5933..5977)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6162..6395
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6892..7017
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7064..7697
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7865..7999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7889..7908)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7943..7962
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8047..8151
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8152..8220,8221..9012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8370..8389)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9183..9771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9672..9691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9925..9942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10059..10080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10095..10100,10101..10118,10119..10125)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10133..10149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10138..10160
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10157..10173
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10157..10173
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tagcaatgtc aaggcctctc actctctgat attcatttct ttgcaagtta taaatactga
       61 ataataagat gacatgaact actactgcta gagattttcc acactgacta aaagggtctg
      121 agggatctct agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa
      181 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
      241 cagatctggt ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga
      301 tctgagggct cgccactccc cagtcccgcc caggccacgc ctccctggaa agtccccagc
      361 ggaaagtccc ttgtagcaag ctcgatatca gcagttcttg aagtactccg gatgcagctc
      421 tcgggccacg tgatgaaatg ctaggcggct gtcaaacctc cactctaaca cttctctctc
      481 cgggtcatcc atcccatgca ggctcacagg gtgtaacaag ctggtgttct ctcctttatt
      541 ggcctcttct accttatctg gctcaactgg tactagcttg tagcaccatc caaaggtcag
      601 tggatatctg acccctggcc ctggtgtgta gttctgctaa tcagggaagt agccttgtgt
      661 gtggtagatc cacagatcaa ggatatcttg tcttcgttgg gagtgaatta gcccttccag
      721 tcccctcttt tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct
      781 taaaggtacc tgaggtgtga ctggaaaacc cacctcctcc tcctcttgtg cttctagcca
      841 ggcacaatca gcattggtag ctgctgtatt gctacttgtg attgctccat gtttttctag
      901 gtctcgactg cagaattaat tccaggcggg gaggcggccc aaagggagat ccgactcgtc
      961 tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga
     1021 aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag
     1081 gtggcaacac aggcgagcag ccatggaaag gacgtcagct tccccgacaa caccacggaa
     1141 ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt
     1201 tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg
     1261 gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc
     1321 ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag
     1381 aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca
     1441 catagcgtaa aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa
     1501 tccagaggtt gattgttcca gacgcggtca ggcaccgggc ttgcgggtca tgcaccaggt
     1561 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
     1621 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
     1681 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac
     1741 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
     1801 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
     1861 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
     1921 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
     1981 cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta
     2041 gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag
     2101 gcgcaccgtg ggcttgtact cggtcatggt aagcttgggc tgcaggtcga aaggcccgga
     2161 gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg
     2221 cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag cccgcccccg
     2281 gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg ctattggccg
     2341 ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg cacgagacta
     2401 gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg
     2461 aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa gaacggagcc
     2521 ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc
     2581 caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa aagcgcctcc
     2641 cctacccggt agaattatct agagcatgtc ggaattctta gctaatcttc ttgaacagcc
     2701 gccagccgct cacagaacct ccagagccac cgctgcccga gcctgtcctt gacaagcctc
     2761 gattatccag gtcctttacc ttgtatatcc tgaccagcca atgttctgtg gtatatgctt
     2821 cctccaggac atcaagctca aagtctttat tcccaatctc agcatttcgg acacggtcaa
     2881 agcctggagg acgcttggct tctgtgtaaa cctgtccaaa gcgatagtaa cacatcttgt
     2941 acatgaggca gttgagcagc actggagaac cttcacggtc cacacggaac tccccagttg
     3001 gagtataata gtcattctcc ttgatatgtt tgcctgtatc tgtgctccct ccaatccgga
     3061 ccatccaaag aaacttgttg atatcatcag aggaataccc agtgaggcct ccaaaaatga
     3121 ccagcacata gctgacatcg agctccctca tgatctcata ggctttttcc tctgtggacg
     3181 ccattgcctg ccctactcga gaaatatggg tattattcca tgtgttattg tccactaaaa
     3241 ttgttcggtt tgccatagct gtaatctgat agccataatc ccaccaggac atgaccttcg
     3301 catcctctgg agtattatga cgaagccaat aatatgcttc tcggaagtca tcaaatatga
     3361 tcctactgcc atccccacca cgggcagata gtacaatgga cggagaagag taggcctcac
     3421 tggtcaccca ggttgaatga aaggtgtagg tgatgagaaa gaaagccatg accagtatca
     3481 tcccacttgc cacttcattc ttaatagggt aggtggaatc ctgttgcttc ttgctcttct
     3541 tgtctggacg acttatgtcc agattcttca tgtatgtgga cagcacctgg gagactccaa
     3601 tgccagagag aatgcacata acaggtgcca acactagcat tagacgcacc attacagctg
     3661 aaaagtacat gctggtcaca ccatacatga tgataaaaat ccgggcatca gacaggttgc
     3721 taaagcagta atacagacca actggaaaca tgaagacgag tagctgcagg tcaaaatagt
     3781 atgaggacca ggttgtgggc tgatgctcag acacagaagc aatgatgggg atgttgttct
     3841 tagcataaga gggatctagc agcgagtaga aacgccccgt ccagggagat atttttcctg
     3901 tcagcatgag gagagctccc acggtgagaa ggacaaagcc taccagagag atgacgctcc
     3961 ggaaaagaac ttcaaattgt tgtggattca acttgctgcg caggtaatcc acaaaggcat
     4021 ggatctggca gagaccaaag accccaaagg ctgccatgtg ctctgatgaa aggacaggct
     4081 ggaaacccac aaaggagatc tgcatagaaa gtatagtgcc caggcagtaa acagtacagt
     4141 aggccacata gatccggtga gagaaacggc ctgtgagcat cagcacgagg acgtggagag
     4201 gaattaagtt gatcaggaac acataacctc cccatgacga gaccatgtag aaataagcaa
     4261 gggcacactt agctgcccaa cagatggaac cagtctttac tgccttgatc cacatgtagt
     4321 aggtgagtag catgcaaaag atggcaatcc cttcattatc ataggagcca gccacagatc
     4381 gggagatata tccaggaact acagcaatca tggcagcagc aagaagccca gcccctgcat
     4441 ccttgagctc tttggtaagg tggtacgtga cgatcgttgt gaaggaggag aagagagggg
     4501 ccaggaacac acagacattc cgaatgtcga tggtgatgtg gaaaaaatgg agtacatggt
     4561 agattgcagc agaggtgatc attaaacctg ggtaaattgt tcctccaatg attcgtccca
     4621 aagggtacca ggctcggtca tcaaaccagt tatggaattt ataaaacccc tcctcagcca
     4681 ggaacctggt agtccgataa ttaaagtacg gatcaaactc atggataaca ctttcaaatc
     4741 tcaggacagc aaacagacga gtggagaagg ataatacagc agccattgac agaatgagaa
     4801 gcttcaaaag tgtgtcctgc ttctcatagg acaatcgcaa aaatccaaac ttagtcatgg
     4861 tggcggatcc cgatgagctc gagatctgag tccggtagcg ctagcgatcc tctagtagag
     4921 tcggtgtctt ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt
     4981 cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     5041 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     5101 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     5161 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     5221 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     5281 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     5341 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     5401 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     5461 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactcccaag cttatcgata
     5521 aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt
     5581 ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact
     5641 gtacccccca atcccccctt ttcttttaaa ggcgataccg tcgagatccg ttcactaatc
     5701 gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg
     5761 gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc
     5821 tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca
     5881 ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg
     5941 cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt
     6001 caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg
     6061 tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag
     6121 cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta
     6181 ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt
     6241 gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac
     6301 cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc
     6361 ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct
     6421 gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt
     6481 tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga
     6541 tcagcggccg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt
     6601 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     6661 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     6721 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
     6781 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     6841 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     6901 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     6961 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     7021 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
     7081 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     7141 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     7201 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaaagc
     7261 agctgcttat atgcaggatc tgagggctcg ccactcccca gtcccgccca ggccacgcct
     7321 ccctggaaag tccccagcgg aaagtccctt gtagcaagct cgatatcagc agttcttgaa
     7381 gtactccgga tgcagctctc gggccacgtg atgaaatgct aggcggctgt caaacctcca
     7441 ctctaacact tctctctccg ggtcatccat cccatgcagg ctcacagggt gtaacaagct
     7501 ggtgttctct cctttattgg cctcttctac cttatctggc tcaactggta ctagcttgta
     7561 gcaccatcca aaggtcagtg gatatctgac ccctggccct ggtgtgtagt tctgctaatc
     7621 agggaagtag ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc ttctttggga
     7681 gtgaattagc ccttccaact actaagtttg tagtacatat ttaacaaata caatttcttt
     7741 aaaatgaaaa taattcagag gaatcacagg tttagagtaa atgaaaccac aggtaattgg
     7801 cagtggtaat agggtatggg gtgggaagtt tgggatgatt ttggttagct tgagttatcc
     7861 agttgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     7921 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     7981 aagctgcaat aaacaagttg atctcccgat ccgtcgacgt caggtggcac ttttcgggga
     8041 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8101 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8161 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8221 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8281 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8341 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8401 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8461 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8521 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8581 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8641 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8701 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8761 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8821 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8881 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8941 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9001 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9061 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9121 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9301 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9781 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9841 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     9961 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    10021 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    10081 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    10141 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
    10201 gtcgacggta aaacgc
//
