LOCUS       sequence-491516-       10687 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  380-bp DIAL (YB_TATA) Reporter Lentivirus (with divergent SNAP) 
            expressing mCherry-HRasG12V in the presence of ZFa and editable by 
            Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, 
            Love KS, Ploessl DS, Galloway KE
  TITLE     Programmable promoter editing for precise control of transgene 
            expression.
  JOURNAL   Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y.
  PUBMED    41083901
REFERENCE   2  (bases 1 to 10687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y."
COMMENT     Sequence Label: 
            LentiX_((380-bp-DIAL)-mCh-HRasG12V-BGH)rev_DIV_pKG3848
FEATURES             Location/Qualifiers
     source          1..10687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    3002..3226
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(join(3386..3919,3920..3922,3923..3955))
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     CDS             complement(3986..4693)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4261..4280)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4530..4548
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    4800..4833
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     4861..4880
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5094..5113
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    5214..5247
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5610..5629)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     promoter        5739..6920
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5969..6911
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6868..6888
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6922..6946
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6976..6985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6982..7527
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             7549..7569
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7583..8171
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7636..7656)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8054..8065)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8263..8496
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8568..8689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8605..8624)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8659..8678
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8729..8864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8884..8903)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8885..8903)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8913..8930)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8913..8929)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8922..8944)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9071..9526
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9158..9177)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9368..9389
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9552..9656
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9657..9725,9726..10517)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9875..9894)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaattcaaa attttatcgt ccagtgtggt ggctactagc
     3001 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     3061 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     3121 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     3181 acgggggagg ggcaaacaac agatggctgg caactagaag gcacaggtgc aacgttgtta
     3241 ggagagcaca cacttgcagc tcatgcagcc ggggccactc tcatcaggag ggttcagctt
     3301 ccgcagcttg tgctgccgga tctcacgcac caacgtgtag aaggcatcct ccactccctg
     3361 ccgggtcttg gccgaggtct cgatgttagg agagcacaca cttgcagctc atgcagccgg
     3421 ggccactctc atcaggaggg ttcagcttcc gcagcttgtg ctgccggatc tcacgcacca
     3481 acgtgtagaa ggcatcctcc actccctgcc gggtcttggc cgaggtctcg atgtagggga
     3541 tgccgtagct tcgggcgagg tcctgagcct gccgagattc cacagtgcgt gcagccaggt
     3601 cacacttgtt ccccaccagc accatgggca cgtcatccga gtccttcacc cgtttgatct
     3661 gctccctgta ctggtggatg tcctcaaaag acttggtgtt gttgatggca aacacacaca
     3721 ggaagccctc cccggtgcgc atgtactggt cccgcatggc gctgtactcc tcctggccgg
     3781 cggtatccag gatgtccaac aggcacgtct ccccatcaat gaccacctgc ttccggtagg
     3841 aatcctctat agtggggtcg tattcgtcca caaaatggtt ctggatcagc tggatggtca
     3901 gcgcactctt gcccacaccg acggcgccca ccaccaccag cttgtattcg gtcatcgaac
     3961 ctcctcctcc gcttccccca ccacccttgt acagctcgtc catgccgccg gtggagtggc
     4021 ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt
     4081 ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg
     4141 tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct
     4201 cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg
     4261 tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt
     4321 agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct
     4381 cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg
     4441 ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga
     4501 tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc
     4561 cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc
     4621 cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc
     4681 ccttgctcac cattgaatga ttgctagcga tgagctctgg tagtagacta gtggccccca
     4741 ttatataccc tctagagact ataatgctta gagtggcttg cttgatcaga tcaaatccga
     4801 taacttcgta taatgtatgc tatacgaagt tatttatgtg tcggggctgg cttaactatg
     4861 cggcatcaga gcagattgta ctgagagtgc accattcgac gctctccctt atgcgactcc
     4921 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     4981 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     5041 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     5101 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc agtcccgggc
     5161 ccgcggtacc gtcgactgca gaattcaatt taaatcgtac cgagcgactc gagataactt
     5221 cgtataatgt atgctatacg aagttattcg gatttgatcc agagtttacg agggtatgta
     5281 gcgaattcgg atttgatcca gaggagggca cgtcctcact ctacacgtcc tcactccgca
     5341 cgtcctcact ctacacgtcc tcactccgca cgtcctcact ctacacgtcc tcactccaat
     5401 tcagatctcc cgatccgagt aagtagcagt ataatgagta cgaacatggt agctagtgga
     5461 tccttcgtcg acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga
     5521 acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt
     5581 ccccgataac ggagaccggc acactggcgc atatcggtgg tcatcatgcg ccagctttca
     5641 tccccgatat gcaccaccgg gtaaagttca cgcatatgaa gctggacatc acctcccaca
     5701 acctcgaggt ggaacagtac gctgatatct cttatactgc tccggtgccc gtcagtgggc
     5761 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     5821 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     5881 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     5941 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     6001 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacgcccc tggctgcagt
     6061 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     6121 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcttgggcgc tggggccgcc
     6181 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     6241 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     6301 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     6361 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     6421 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     6481 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     6541 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     6601 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     6661 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     6721 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     6781 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     6841 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     6901 ttccatttca ggtgtcgtga gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     6961 tccacgctgt aatgagccac catggacaaa gactgcgaaa tgaagcgcac caccctggat
     7021 agccctctgg gcaagctgga actgtctggg tgcgaacagg gcctgcaccg tatcatcttc
     7081 ctgggcaaag gaacatctgc cgccgacgcc gtggaagtgc ctgccccagc cgccgtgctg
     7141 ggcggaccag agccactgat gcaggccacc gcctggctca acgcctactt tcaccagcct
     7201 gaggccatcg aggagttccc tgtgccagcc ctgcaccacc cagtgttcca gcaggagagc
     7261 tttacccgcc aggtgctgtg gaaactgctg aaagtggtga agttcggaga ggtcatcagc
     7321 tacagccacc tggccgccct ggccggcaat cccgccgcca ccgccgccgt gaaaaccgcc
     7381 ctgagcggaa atcccgtgcc cattctgatc ccctgccacc gggtggtgca gggcgacctg
     7441 gacgtggggg gctacgaggg cgggctcgcc gtgaaagagt ggctgctggc ccacgagggc
     7501 cacagactgg gcaagcctgg gctgggtgag ccagcgaatt cctgcaggcc caagaaaaaa
     7561 cgcaaggtgt agcaacgccg ataatcaacc tctggattac aaaatttgtg aaagattgac
     7621 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     7681 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7741 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7801 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7861 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7921 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7981 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     8041 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     8101 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     8161 cgcctccccg ccgctcgctg atatagggcc cggtaccttt aagaccaatg acttacaagg
     8221 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact
     8281 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     8341 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8401 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8461 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     8521 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     8581 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8641 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     8701 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8761 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8821 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     8881 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     8941 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     9001 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     9061 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     9121 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     9181 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     9241 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     9301 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     9361 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     9421 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     9481 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     9541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9601 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     9661 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     9721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     9781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     9841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     9901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     9961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    10021 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    10081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    10141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    10201 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    10261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    10321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    10381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    10441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    10561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    10621 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    10681 gatcttc
//
