LOCUS       sequence-491519-        7143 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  GIB-Cre Retrovirus expressing N-terminal giberellin inducible Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, 
            Love KS, Ploessl DS, Galloway KE
  TITLE     Programmable promoter editing for precise control of transgene 
            expression.
  JOURNAL   Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y.
  PUBMED    41083901
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y."
COMMENT     Sequence Label: pMXs-GIB-Cre-N-WPRE_pKG3943
FEATURES             Location/Qualifiers
     source          1..7143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     117..133
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    222..246
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2090..2110
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2124..2148)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2165..2753
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2218..2238)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2636..2647)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2933..3402
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3532..3549)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3703..4291)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3783..3802)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4455..5246,5247..5315))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5078..5097
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5316..5420)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5450..6043
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6106..6463
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             6522..6938
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6868..6890
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6906..6922
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    join(6948..7143,1..179)
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
ORIGIN
        1 tcggcgacac agtgtgggtc cgccgacacc agactaagaa cctagaacct cgctggaaag
       61 gaccttacac agtcctgctg accaccccca ccgccctcaa agtagacggc atcgcagctt
      121 ggatacacgc cgcccacgtg aaggctgccg accccggggg tggaccatcc tctagactgc
      181 cggatctagc tagttaatcg acggtatcga taagctatca aacaagtttg tacaaaaaag
      241 caggctccac catgggaacc aattcagtcg actggatccg gtactactag gctgtctcgt
      301 ctcgtctcgc acgcgtgccg ccaccatgtc caacctgctg actgtgcacc aaaacctgcc
      361 tgccctccct gtggatgcca cctctgatga agtcaggaag aacctgatgg acatgttcag
      421 ggacaggcag gccttctctg aacacacctg gaagatgctc ctgtctgtgt gcagatcctg
      481 ggctgcctgg tgcaagctga acaacaggaa atggttccct gctgaacctg aggatgtgag
      541 ggactacctc ctgtacctgc aagccagagg cctggctgtg aagaccatcc aacagcacct
      601 gggccagctc aacatgctgc acaggagatc tggcctgcct cgcccttctg actccaatgc
      661 tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg agagagccaa
      721 gcaggccctg gcctttgaac gcactgactt tgaccaagtc agatccctga tggagaactc
      781 tgacagatgc caggacatca ggaacctggc cttcctgggc attgcctaca acaccctgct
      841 gcgcattgcc gaaattgcca gaatcagagt gaaggacatc tcccgcaccg atggtgggag
      901 aatgctgatc cacattggca ggaccaagac cctggtgtcc acagctggtg tggagaaggc
      961 cctgtccctg ggggttacca agctggtgga gagatggatc tctgtgtctg gttccggagg
     1021 gtctggctcc ggatcaagtg gtggcagcgg taccatggcc gccagcgacg aagtgaacct
     1081 gatcgagagc agaaccgtgg tgcccctgaa cacctgggtg ctgatctcca acttcaaggt
     1141 ggcctacaac atcctgcgga ggcccgacgg caccttcaac agacacctgg ccgagtacct
     1201 ggaccggaaa gtgaccgcca acgccaaccc tgtggacggc gtgttcagct tcgacgtgct
     1261 gatcgaccgg cggatcaacc tgctgagccg ggtgtacaga cccgcctacg ccgatcagga
     1321 acagcccccc tctatcctgg atctggaaaa gcccgtggat ggcgacatcg tgcccgtgat
     1381 cctgttcttc cacggcggca gctttgccca cagcagcgcc aatagcgcca tctacgacac
     1441 cctgtgcaga cggctcgtgg gcctgtgcaa atgcgtggtg gtgtccgtga actaccgcag
     1501 agcccccgag aacccttacc cctgcgccta cgatgatggc tggatcgccc tgaactgggt
     1561 caacagcaga agctggctga agtccaagaa agacagcaag gtgcacatct ttctggccgg
     1621 cgatagcagc ggcggcaata tcgcccataa cgtggccctg agagccggcg agtctggcat
     1681 cgatgtgctg ggcaatatcc tgctgaaccc catgttcggc ggcaacgagc ggaccgagag
     1741 cgagaagtct ctggacggca agtacttcgt gaccgtgcgg gaccgggact ggtactggaa
     1801 ggcctttctg cccgagggcg aggacagaga gcaccccgcc tgcaatccct tcagccccag
     1861 aggcaaaagc ctggaaggcg tgtccttccc aaagtccctg gtggtggtgg ccggcctgga
     1921 cctgatcaga gattggcagc tggcctatgc cgagggcctg aagaaagccg gccaggaagt
     1981 gaagctgatg cacctggaaa aggccaccgt gggcttctac ctgctgccca acaacaacca
     2041 cttccacaac gtgatggacg agatcagcgc cttcgtgaac gccgagtgcc ccaagaaaaa
     2101 gcggaaggtg cgctgatatc tagacccagc tttcttgtac aaagtggttc gatagcttat
     2161 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2221 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     2281 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     2341 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     2401 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     2461 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     2521 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     2581 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     2641 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     2701 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     2761 ccgtcgacga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     2821 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     2881 acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat
     2941 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     3001 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     3061 gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat
     3121 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     3181 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     3241 ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
     3301 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     3361 tctcctctga gtgattgact acccgtcagc gggggtcttt cacatgcagc atgtatcaaa
     3421 attaatttgg ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat
     3481 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3541 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3601 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3661 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3721 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3781 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3841 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3901 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3961 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4021 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4081 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4141 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4201 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4261 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4321 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4381 gatcttcacc tagatccttt tgcggccggc cgcaaatcaa tctaaagtat atatgagtaa
     4441 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4501 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4561 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4621 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4681 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4741 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4801 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4861 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4921 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4981 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5041 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     5101 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5161 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5221 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     5281 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     5341 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5401 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgcata atgaaagacc
     5461 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
     5521 cataactgag aatagaaaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
     5581 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     5641 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     5701 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
     5761 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     5821 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     5881 cacaacccct cactcggcgc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
     5941 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     6001 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
     6061 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
     6121 ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta
     6181 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttcggaac
     6241 acccggccgc aaccctggga gacgtcccag ggacttcggg ggccgttttt gtggcccgac
     6301 ctgagtccaa aaatcccgat cgttttggac tctttggtgc acccccctaa taggagggat
     6361 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
     6421 tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt
     6481 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
     6541 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     6601 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     6661 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     6721 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     6781 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     6841 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     6901 cctcccttta tccagccctc actccttctc taggcgcccc catatggcca tatgagatct
     6961 tatatggggc acccccgccc cttgtaaact tccctgaccc tgacatgaca agagttacta
     7021 acagcccctc tctccaagct cacttacagg ctctctactt agtccagcac gaagtctgga
     7081 gacctctggc ggcagcctac caagaacaac tggaccgacc ggtggtacct cacccttacc
     7141 gag
//
