LOCUS       sequence-491528-        8815 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  Lentiviral-based expression of EGFP with an ER retention signal 
            sequence in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Lampson BL, Ramiotarez AS, Baro M, He L, Hegde M, Koduri V, Pfaff 
            JL, Hanna RE, Kowal J, Shirole NH, He Y, Doench JG, Contessa JN, 
            Locher KP, Kaelin WG Jr
  TITLE     Positive selection CRISPR screens reveal a druggable pocket in an 
            oligosaccharyltransferase required for inflammatory signaling to 
            NF-kappaB.
  JOURNAL   Cell. 2024 Apr 25;187(9):2209-2223.e16. doi: 
            10.1016/j.cell.2024.03.022.
  PUBMED    38670073
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.03"; journalName: "Cell"; date: "2024-04-25- 
            25"; volume: "187"; issue: "9"; pages: "2209-2223.e16"
COMMENT     Sequence Label: ER-GFP pLVX CMVpuro
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    669..794
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1291..1524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1709..1753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2016..2131
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2189..2492
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2493..2696
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2647..2667
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2693..2717
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2879..2881,2882..3592)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2879..2881,2882..3592)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2921..2942)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3182..3201)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3529..3550
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3638..4137
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3673..3696)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4070..4089
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4158..4757
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4158..4177)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4654..4674
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4771..5359
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4824..4844)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5242..5253)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5566..6199
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6328..6344)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6328..6344)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6341..6363)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6352..6368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6376..6382,6383..6400,6401..6406))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6421..6442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6559..6576)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6730..7318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6810..6829)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7489..8280,8281..8349))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8112..8131
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8350..8454)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8502..8636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8539..8558)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8593..8612
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(8804..8815,1..622)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
       61 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
      121 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
      181 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
      241 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
      301 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
      361 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
      421 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
      481 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      541 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
      601 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
      661 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
      721 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
      781 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
      841 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
      901 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
      961 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     1021 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     1081 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
     1141 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     1201 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     1261 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     1321 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     1381 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     1441 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     1501 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     1561 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     1621 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     1681 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     1741 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     1801 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     1861 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     1921 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     1981 cattcgatta gtgaacggat ctcgacggta tcgcctttaa aagaaaaggg gggattgggg
     2041 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2101 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2161 cagtttatcg ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     2221 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     2281 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     2341 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     2401 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     2461 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2521 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2581 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2641 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     2701 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     2761 accgactcta ctagaggatc gctagcgcta ccggactcag atctcgagct catcgggatc
     2821 cgccaccatg ctgctatccg tgccgctgct gctcggcctc ctcggcctgg ccgtcgccgt
     2881 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2941 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3001 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3061 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     3121 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3181 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3241 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3301 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     3361 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     3421 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3481 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3541 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agaaggatga
     3601 actttaagaa ttccgacatg ctctagataa ttctaccggg taggggaggc gcttttccca
     3661 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     3721 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     3781 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     3841 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     3901 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     3961 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     4021 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     4081 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     4141 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     4201 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     4261 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     4321 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     4381 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     4441 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     4501 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     4561 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     4621 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     4681 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     4741 gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa aatttgtgaa
     4801 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4861 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4921 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4981 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5041 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5101 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5161 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     5221 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5281 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5341 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
     5401 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     5461 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     5521 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc
     5581 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     5641 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     5701 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     5761 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     5821 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     5881 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     5941 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     6001 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6061 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6121 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6181 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6241 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac
     6301 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6361 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6421 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6541 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6601 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6661 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6781 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6841 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6901 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6961 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7021 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7081 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7141 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7201 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7261 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7321 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7381 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7441 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7501 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7561 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7621 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7681 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7741 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7801 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7861 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7921 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7981 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8041 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8101 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8161 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8221 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8281 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8341 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8461 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
     8521 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8581 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     8641 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
     8701 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
     8761 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaa
//
