LOCUS       sequence-491531-        9932 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  HA-tagged mouse CDK1 cloned into the pCW backbone (Addgene #41393, 
            with puromycin resistance) together with an IRES GFP to indicate 
            doxycycline-inducible expression by fluorescence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9932)
  TITLE     Doxycycline-inducible pCW vectors with HA-tagged genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW-CDK1-HA
FEATURES             Location/Qualifiers
     source          1..9932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        73..144
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(145..213,214..1005)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(363..382)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1176..1764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1665..1684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1918..1935
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2052..2073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2088..2093,2094..2111,2112..2118)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2126..2142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2131..2153
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2150..2166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2150..2166
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2185..2205
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2187..2205
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2233..2459
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2460..2640
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2687..2812
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3305..3538
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3723..3767
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3980..4097
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4151..4465
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4159..4177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4195..4213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4230..4248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4266..4284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4302..4320
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4337..4355
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4373..4391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4438..4462
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4482..4491
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5382..5408
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5412..5438
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5507..6073
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5523..6075
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5667..5684)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5894..5913
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6087..6089,6090..6092,6093..6806)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6087..6089,6090..6092,6093..6803)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6132..6153)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6393..6412)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6740..6761
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6813..7323
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7242..7260
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7333..7929
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7333..7352)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7829..7849
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7930..7983
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7984..8601,8602..8607,8608..8643,8644..8646,8647..8682,
                     8683..8685,8686..8721,8722..8730)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(8770..8790)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9188..9199)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9404..9637
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9709..9830
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9746..9765)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9800..9819
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      join(9870..9932,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
       61 ttttttggag gcctttcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      121 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      181 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      241 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      301 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      361 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      421 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      481 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      541 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      601 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      661 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
      721 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      781 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      841 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      901 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
      961 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1021 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1081 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1141 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1201 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1261 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1321 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1381 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1441 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1501 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1561 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1621 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1681 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1741 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1801 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1861 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1921 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     1981 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2041 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     2101 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     2161 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     2221 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     2281 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     2341 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     2401 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     2461 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     2521 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     2581 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     2641 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     2701 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     2761 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     2821 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     2881 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     2941 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     3001 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     3061 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     3121 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     3181 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     3241 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     3301 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     3361 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     3421 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     3481 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     3541 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     3601 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     3661 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     3721 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     3781 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     3841 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     3901 cattatcgtt tcagacccac ctcccaaccc cgaggggaca attctcgacc tcgagacaaa
     3961 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     4021 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     4081 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcgaa
     4141 tcgatatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     4201 tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
     4261 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     4321 acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     4381 agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
     4441 tcgtttagtg aaccgtcaga tcgcctggag aattggctag cgccaccatg gaagactata
     4501 tcaaaataga gaaaattgga gaaggtactt acggtgtggt gtataagggt agacacagag
     4561 tcactggcca gatagtggcc atgaagaaga tcagacttga aagcgaggaa gaaggagtgc
     4621 ccagtactgc aattcgggaa atctctctat taaaagaact tcgacatcca aatatagtca
     4681 gcctgcagga tgtgctcatg caggactcca ggctgtatct catctttgag ttcctgtcca
     4741 tggacctcaa gaagtacctg gactccatcc ctcctgggca gttcatggat tcttcactcg
     4801 ttaagagtta cttacaccaa atcctccagg gaattgtgtt ttgccactcc cggcgagttc
     4861 ttcacagaga cttgaaacct caaaatctat tgattgatga caaaggaaca atcaaactgg
     4921 ctgatttcgg ccttgccaga gcgtttggaa taccgatacg agtgtacaca cacgaggtag
     4981 tgacgctgtg gtaccgatct ccagaagtgt tgctgggctc ggctcgttac tccactccgg
     5041 ttgacatctg gagtataggg accatatttg cagaactggc caccaagaag ccgcttttcc
     5101 acggcgactc agagattgac cagctcttca ggatcttcag agctctgggc actcctaaca
     5161 acgaagtgtg gccagaagtc gagtccctgc aggactacaa gaacaccttt cccaagtgga
     5221 agccggggag cctcgcatcc cacgtcaaga acctggacga gaacggcttg gatttgctct
     5281 caaaaatgct agtctatgat cctgccaaac gaatctctgg caaaatggcc ctgaagcacc
     5341 cgtactttga tgacttggac aatcagatta agaagatggg atacccatac gatgttcctg
     5401 actatgcggg ctatccctat gacgtcccgg actatgcagg gccggctaag gcagcggcac
     5461 gggcggtgta gctcgagggc cggcgcgccg cggccgctac gtaaattccg cccccccccc
     5521 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     5581 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     5641 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     5701 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     5761 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     5821 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     5881 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     5941 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     6001 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     6061 aacacgatga taatatggcc acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     6121 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     6181 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     6241 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     6301 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     6361 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     6421 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     6481 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     6541 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     6601 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     6661 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     6721 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     6781 tcggcatgga cgagctgtac aagtaaggat ccggggttgg ggttgcgcct tttccaaggc
     6841 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     6901 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     6961 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     7021 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     7081 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     7141 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     7201 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     7261 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     7321 cagcaattca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     7381 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     7441 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     7501 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     7561 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     7621 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     7681 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     7741 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     7801 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     7861 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     7921 cccggtgccg aaggtagagg ttctctcctc acttgtggtg atgttgaaga aaaccctggt
     7981 ccaatgtcta gactggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt
     8041 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
     8101 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg
     8161 ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg
     8221 cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
     8281 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
     8341 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
     8401 tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag
     8461 acacctacca ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg
     8521 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
     8581 cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg
     8641 ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac
     8701 gattttgacc ttgacatgct ccccgggtaa ctaagtaagg atcgatccaa gatatcgtat
     8761 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     8821 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     8881 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     8941 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     9001 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     9061 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     9121 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     9181 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     9241 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     9301 cccgcctgtt tcgcctcggc gtccggacta gaggtacctt taagaccaat gacttacaag
     9361 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     9421 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     9481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     9541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     9601 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     9661 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     9721 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9781 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     9841 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     9901 attctccgcc ccatggctga ctaatttttt tt
//
