LOCUS       sequence-491537-       12101 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  siRNA resistant HA-tagged mouse MRTF-A cloned into the pCW backbone 
            (Addgene #41393, with puromycin resistance) together with an IRES 
            GFP to indicate doxycycline-inducible expression by fluorescence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12101)
  TITLE     Doxycycline-inducible pCW vectors with HA-tagged genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW-MRTFA-HA
FEATURES             Location/Qualifiers
     source          1..12101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3612..3638
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3642..3668
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3737..4303
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3753..4305
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3897..3914)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4124..4143
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4317..4319,4320..4322,4323..5036)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4317..4319,4320..4322,4323..5033)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4362..4383)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4623..4642)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4970..4991
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5043..5553
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5472..5490
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5563..6159
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5563..5582)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6059..6079
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6160..6213
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6214..6831,6832..6837,6838..6873,6874..6876,6877..6912,
                     6913..6915,6916..6951,6952..6960)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7000..7020)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7418..7429)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7634..7867
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7939..8060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7976..7995)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8030..8049
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8100..8235
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8235..8306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8307..8375,8376..9167)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8525..8544)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9338..9926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9827..9846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10080..10097
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10214..10235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10250..10255,10256..10273,10274..10280)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10288..10304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10293..10315
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10312..10328
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10312..10328
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10347..10367
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10349..10367
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10395..10621
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10622..10802
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10849..10974
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11467..11700
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11885..11929
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat gcccccttcc gtcattgctg tgaatgggct ggacggagga ggggctggcg
      601 aaaatgacga cgagccagtg ctcctgtctc tgtctgcggc ccccagcccc cagagcgaag
      661 ctgttgccaa tgaactgcag gagctgtccc tgcagcccga gctgactcta ggcctccatc
      721 ctgggaggaa ccccaattta cctccactta gtgagcggaa gaatgtgctg cagttgaagc
      781 tccagcagcg gcggacccgg gaggaactgg tgagccaagg gatcatgccg cctttgaaaa
      841 gccccgctgc atttcatgag cagagaagaa gcctggagcg ggccaggacc gaggactatt
      901 tgaaacggaa gatccgttcc cggcccgaga gagcagagct ggtcaggatg cacattctgg
      961 aagagacctc ggctgagcct tcgctccagg ccaagcagct gaagctgaag agagccaggc
     1021 tggctgatga cctcaatgaa aagattgcac agaggcctgg ccccatggaa ctagtagaaa
     1081 agaatatcct gcctgtggag tccagcctga aggaggctat cattgtgggc caggtaaatt
     1141 acccaaaggt agcagacagt tcctccttcg acgaggacag cagcgatgcc ctgtctcctg
     1201 agcagcctgc cagccatgag tcccagggtt cagtgccatc acccttggag tcccgagtca
     1261 gtgatccact gcccagtgcc acctccatat cacccactca ggttctttct caactcccaa
     1321 tggctccgga tcctggagag acgctttttc tggcagagca gcctcctctg cctcccgcac
     1381 ctctgctgcc cccaagccta gccaatggaa gcatcgtccc cactgccaag cctgctccca
     1441 cactcatcaa gcaaagccaa cccaagtctg ccagcgagaa atcacagcgc agcaagaagg
     1501 ccaaggagct gaagccaaag gtgaagaagc tcaagtacca ccagtacatc cccccggacc
     1561 agaagcagga caagggggcg cccgccatgg actcctccta tgccaagatc ctgcagcagc
     1621 agcagctctt cctgcagctg cagatcctca accagcagca gcagcagcag cagcaacagc
     1681 actacaacta ccaggccatc ctgcctgccc ctcccaagcc ctcggctgag actcctggaa
     1741 gcagtgcccc taccccatca cgcagcctct ccaccagtag cagccccagc tcaggcaccc
     1801 cagggcccag cgggctggca cgccagagca gcaccgcact agctgccaaa ccaggagccc
     1861 tgccagccaa cctggatgac atgaaggtgg cagagctgaa gcaagaactg aagttgcggt
     1921 cccttcccgt ctcaggcacc aagacagagc tgatagaacg cctgcgtgcc taccaagacc
     1981 aagtcagccc agctccagga gcccccaagg cccctgccac cacctctgtg ctgtccaagg
     2041 ctggtgaggt agtggtcgcc ttccctgcgg ccctgctaag cacagggtca gctcttgtaa
     2101 cagcaggcct tgcaccagct gagatggtgg tggccacagt aaccagcaat ggcatggtga
     2161 agtttggcag cacaggctcc acaccccccg tgtctcccac cccttcagag cgctcactgc
     2221 tcagcacggg tgatgagaat tccacacctg gggatgcctt tggtgaaatg gtgacatcgc
     2281 cgctgacaca gctcaccctg caggcctccc cactgcagat cgtgaaggag gagggtgccc
     2341 gtgctgcgtc ctgctgtcta agccctggtg ctcgggctga gctggaggga ctggacaagg
     2401 accagatgct gcaggagaag gacaagcaga ttgaggagct gacccgaatg ctccaacaga
     2461 agcagcagct ggttgagctg ctgcggctac agctggagca gcagaagcgg gcccagcagc
     2521 cagccccagc cagcagccct gtgaagaggg aaagtggttt ctccagttgc cagctgagct
     2581 gccagcccca gggctctgcc catgcttttg gctctggcct agtggttccc actaccaacc
     2641 atggagacac tcaggcccca gcgccagagt ccccacctgt ggtggtgaag caggaagctg
     2701 ggccacctga gccagatctg gcccccagct cccagctgct cttgggctcc cagggcacca
     2761 gcttcctcaa gagggtcagc cctcctaccc tggtcactga ctctacaggg actcacctca
     2821 tcctcactgt gaccaataag agtgctgatg gccctggctt gcctgcaggg agcccccagc
     2881 agcccttgtc ccagcctggt tctccagccc ctggtccacc tgcccagatg gacctggagc
     2941 acccacctca gcctccgttt gcaaccccca catctctgct gaagaaggag ccccctggtt
     3001 atgaagagac tgtgacccag cagcctaagc agcaggaaaa tggctcctcc agtcagcaca
     3061 tggatgatct gtttgatatt cttattcaga gtggagagat ttcagcagat ttcaaagagc
     3121 caccatccct accaggcaag gaaaagtcac ctccagcagc agcagcgtat gggcctccat
     3181 tgacaccaca accctcgcct ttgagtgaac tcccccaagc tgctcctcca ccaggctccc
     3241 ccaccctccc agggcgcctt gaagacttcc tggagagcag cacagggctg cccctgctga
     3301 caagtgggca cgagggacca gaaccccttt ccctcattga tgacctccac agccagatgc
     3361 tgagcagctc cgccatcctg gaccaccccc catcacccat ggacacctct gaattgcact
     3421 ttgctcctga gcccagcagt ggtatgggcc tggacctggc tgttggccac ctggacagca
     3481 tggactggct ggagctgtcg tctggtggcc ctgtgctcag cctggctccc ctcagcactg
     3541 cagcccccag cctcttctcg atggacttcc tggatggaca cgacttgcag ctccactggg
     3601 attcctgctt gtacccatac gatgttcctg actatgcggg ctatccctat gacgtcccgg
     3661 actatgcagg gccggctaag gcagcggcac gggcggtgta gctcgagggc cggcgcgccg
     3721 cggccgctac gtaaattccg cccccccccc taacgttact ggccgaagcc gcttggaata
     3781 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     3841 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     3901 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     3961 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     4021 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     4081 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     4141 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     4201 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     4261 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg
     4321 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4381 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     4441 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4501 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     4561 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4621 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4681 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4741 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     4801 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     4861 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4921 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4981 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaggat
     5041 ccggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     5101 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     5161 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     5221 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     5281 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     5341 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     5401 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     5461 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     5521 ctcgttgacc gaatcaccga cctctctccc cagcaattca ccatgaccga gtacaagccc
     5581 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     5641 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     5701 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     5761 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     5821 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     5881 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     5941 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6001 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6061 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6121 ggaccgcgca cctggtgcat gacccgcaag cccggtgccg aaggtagagg ttctctcctc
     6181 acttgtggtg atgttgaaga aaaccctggt ccaatgtcta gactggacaa gagcaaagtc
     6241 ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa
     6301 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     6361 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     6421 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct
     6481 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     6541 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     6601 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     6661 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     6721 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     6781 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc
     6841 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     6901 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     6961 ctaagtaagg atcgatccaa gatatcgtat tcttaactat gttgctcctt ttacgctatg
     7021 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7081 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7141 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7201 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7261 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7321 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7381 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7441 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7501 gacgagtcgg atctcccttt gggccgcctc cccgcctgtt tcgcctcggc gtccggacta
     7561 gaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7621 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     7681 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7741 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7801 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7861 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7921 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7981 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8041 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8101 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8161 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8221 gcttttttgg aggcctttca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8281 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8341 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8401 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8461 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8521 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     8581 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8641 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8701 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8761 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8821 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8881 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8941 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9001 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9061 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9121 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9181 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9241 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9481 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9961 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10021 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10081 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10141 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    10201 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    10261 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    10321 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    10381 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
    10441 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
    10501 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
    10561 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
    10621 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    10681 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    10741 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    10801 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    10861 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    10921 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    10981 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    11041 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    11101 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    11161 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    11221 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    11281 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
    11341 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
    11401 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
    11461 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
    11521 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
    11581 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
    11641 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
    11701 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
    11761 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
    11821 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
    11881 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
    11941 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
    12001 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
    12061 accattatcg tttcagaccc acctcccaac cccgagggga c
//
