LOCUS       sequence-491548-       10577 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  RSF1010 origin of replication plasmid containing Eco2 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by Pm promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10577)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 10577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010-Eco2
FEATURES             Location/Qualifiers
     source          1..10577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             105..113
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      1559..1653
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1774..1793)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1785..2600
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2767..2875
                     /note="incP origin of transfer"
     CDS             complement(2886..3737)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(3724..4563)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(5074..6045)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      6152..6546
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(6584..6670)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(6695..7660)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        8560..8640
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
ORIGIN
        1 caacctgtcc cttacgaata gctcttcacc ccgaaggagt gcctgcatgc gtgccacctg
       61 catgatccgc ggccgcactc gatcgaaata attttgttta actttaagga ggtttggaat
      121 gaaccaaatc gtgaagttca ctgacgactc tggcctggcg gttcaagtta ctccagacga
      181 tgttcgccgt tatatctgtg agaacgctac tgaaaaagag gtgggcctct ttctgcaact
      241 ctgtcagact caacgtctga atccgtttgt gaaagacgct tacctggtga aatacggcgg
      301 tgctccagct tctatgatta cttcctatca agtttttaac cgtcgcgcgt gtcgtgatgc
      361 taactatgat ggtatcaaat ctggtgtggt tgttctgcgt gacggtgatg ttgtgcataa
      421 acgtggtgct gcgtgctaca aaaaggcggg tgaggagctc atcggtggtt gggcggaagt
      481 tcgctttaag gatggccgcg agactgcgta tgctgaggtg gcgctcgacg actattccac
      541 cggcaaatct aattgggcga aaatgccggg tgttatgatc gaaaaatgcg cgaaggctgc
      601 tgcttggcgc ctcgcgttcc cggacacttt tcagggcatg tacgctgcgg aggaaatgga
      661 tcaagcgcaa cagccagaac aggtgcgcgc tcaggcggag caaccagtgg atctccagcc
      721 aatccgcgaa ctcttcaagc catattgcga acacttcggc atcactccgg ctgagggtat
      781 gactgctgtt tgtggtgcgg tgggcgctga aggcatgcac tctatgaccg agcagcaagc
      841 tcgccgtgct cgcgcttgga tggaggaaga aatggctgcg ccagctgtgg aagcggagta
      901 tgaggttgtt gacgagggcg aggtgtttta aggatccagg agaatacaca tggccagcag
      961 aggcgtaaac aaggttattc tcgttggtaa tctgggtcag gacccggaag tacgctacat
     1021 gccaaatggt ggcgcagttg ccaacattac gctggctact tccgaatcct ggcgtgataa
     1081 agcgaccggc gagatgaaag agcagactga atggcaccgc gttgtgctgt tcggcaaact
     1141 ggcagaagtg gccagcgaat atctgcgtaa aggttctcag gtttatatcg aaggtcagct
     1201 gcgtacccgt aaatggaccg atcaatccgg tcaggatcgc tacaccacag aagtcgtggt
     1261 gaacgttggc ggcaccatgc agatgctggg tggtcgtcag ggtggtggcg ctccggcagg
     1321 tggtaatatc ggtggtggtc agccgcaggg cggttggggt cagcctcagc agccgcaggg
     1381 tggcaatcag ttcagcggcg gcgcgcagtc tcgcccgcag cagtccgctc cggcagcgcc
     1441 gtctaacgag ccgccgatgg actttgatga tgacattccg ttctgatcta gagtcgacct
     1501 gcaggcatgc aagcttgcgg ccgcgtcgtg actgggaaaa ccctggcgac tagtcttgga
     1561 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     1621 ggttgccgcc gggcgttttt tattggtgag aatccagggg tccccaataa ttacgattta
     1681 aatttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     1741 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcagc
     1801 gtgaaacgag ctgtagccgt ccgcgtctga acagcaacat ggatgcggat ctgtatggct
     1861 ataaatgggc gcgtgataac gtgggtcaga gcggcgcgac catttatcgt ctgtatggca
     1921 aaccggatgc gccggaactg tttctgaaac atggcaaagg cagcgtggcg aacgatgtga
     1981 ccgatgaaat ggtgcgtctg aactggctga ccgaatttat gccgctgccg accattaaac
     2041 attttattcg caccccggat gatgcgtggc tgctgaccac cgcgattccg ggcaaaaccg
     2101 cgtttcaggt gctggaagaa tatccggata gcggcgaaaa cattgtggat gcgctggccg
     2161 tgtttctgcg tcgtctgcat agcattccgg tgtgcaactg cccgtttaac agcgatcgtg
     2221 tgtttcgtct ggcccaggcg cagagccgta tgaacaacgg cctggtggat gcgagcgatt
     2281 ttgatgatga acgtaacggc tggccggtgg aacaggtgtg gaaagaaatg cataaactgc
     2341 tgccgtttag cccggatagc gtggtgaccc acggcgattt tagcctggat aacctgattt
     2401 tcgatgaagg caaactgatt ggctgcattg atgtgggccg tgtgggcatt gcggatcgtt
     2461 atcaggatct ggccattctg tggaactgcc tgggcgaatt tagcccgagc ctgcaaaaac
     2521 gtctgtttca gaaatatggc attgataatc cggatatgaa caaactgcaa tttcatctga
     2581 tgctggatga atttttctaa taattaattg gaccgcggtc cgcgcgttgt ccttttccgc
     2641 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc
     2701 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg
     2761 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt
     2821 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccgtaggc
     2881 cggcctcagc ctgccgcctt gggccgggtg atgtcgtact tgcccgccgc gaactcggtt
     2941 accgtccagc ccagcgcgac cagctccggc aacgcctcgc gcacccgctg gcggcgcttg
     3001 cgcatggtcg aaccactggc ctctgacggc cagacatagc cgcacaaggt atctatggaa
     3061 gccttgccgg ttttgccggg gtcgatccag ccacacagcc gctggtgcag caggcgggcg
     3121 gtttcgctgt ccagcgcccg cacctcgtcc atgctgatgc gcacatgctg gccgccaccc
     3181 atgacggcct gcgcgatcaa ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg
     3241 ctggcgtact ccgacagcag ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg
     3301 gataccttcc aaaggcgctc gatgcagtcc tgtatgtgct tgagcgcccc accactatcg
     3361 acctctgccc cgatttcctt tgccagcgcc cgatagctac ctttgaccac catggcatca
     3421 gcggtgacgg cctcccactt gggttccagg aacagccgga gctgccgtcc gccttcggtc
     3481 ttgggttccg ggccaagcac taggccatta ggcccagcca tggccaccag cccttgcagg
     3541 atgcgcagat catcagcgcc cagcggctcc gggccgctga actcgatccg cttgccgtcg
     3601 ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct cgccccgctt gagggcacgg
     3661 aacaggccgg gggccagaca gtgcgccggg tcgtgccgga cgtggctgag gctgtgcttg
     3721 ttcttaggct tcaccacggg gcaccccctt gctcttgcgc tgcctctcca gcacggcggg
     3781 cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt
     3841 ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc
     3901 ctcggcctcg gcgctggtca tgctcgacag gtaggactgc cagcggatgt tatcgaccag
     3961 taccgagctg ccccggctgg cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt
     4021 gctggcatgg tgcaggaaca cgatagagca cccggtatcg gcggcgatgg cctccatgcg
     4081 accgatgacc tgggccatgg ggccgctggc gttttcttcc tcgatgtgga accggcgcag
     4141 cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc
     4201 cggggccatg atgttgggca ggctgccgat cagcggctgg atcagcaggc cgtcagccac
     4261 ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc
     4321 ggtgggcggg tcttcggcgg gcaggtagat caccgggccg gtgggcagtt cgcccacctc
     4381 cagcagatcc ggcccgcctg caatctgtgc ggccagttgc agggccagca tggatttacc
     4441 ggcaccaccg ggcgacacca gcgccccgac cgtaccggcc accatgttgg gcaaaacgta
     4501 gtccagcggt ggcggcgctg ctgcgaacgc ctccagaata ttgataggct tatgggtagc
     4561 cattgattgc ctcctttgca ggcagttggt ggttaggcgc tggcggggtc actacccccg
     4621 ccctgcgccg ctctgagttc ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc
     4681 agccagaact tgcgctgacg catccctttg gccttcatgc gctcggcata tcgcgcttgg
     4741 cgtacagcgt cagggctggc cagcaggtcg ccggtctgct tgtccttttg gtctttcata
     4801 tcagtcaccg agaaacttgc cggggccgaa aggcttgtct tcgcggaaca aggacaaggt
     4861 gcagccgtca aggttaaggc tggccatatc agcgactgaa aagcggccag cctcggcctt
     4921 gtttgacgta taaccaaagc caccgggcaa ccaatagccc ttgtcacttt tgatcaggta
     4981 gaccgaccct gaagcgcttt tttcgtattc cataaaaccc ccttctgtgc gtgagtactc
     5041 atagtataac aggcgtgagt accaacgcaa gcactacatg ctgaaatctg gcccgcccct
     5101 gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc tcggcccgcg caagctggac
     5161 gctgggcaga cccatgacct tgctgacggt gcgctcgatg taatccgctt cgtggccggg
     5221 cttgcgctct gccagcgctg ggctggcctc ggccatggcc ttgccgattt cctcggcact
     5281 gcggccccgg ctggccagct tctgcgcggc gataaagtcg cacttgctga ggtcatcacc
     5341 gaagcgcttg accagcccgg ccatctcgct gcggtactcg tccagcgccg tgcgccggtg
     5401 gcggctaagc tgccgctcgg gcagttcgag gctggccagc ctgcgggcct tctcctgctg
     5461 ccgctgggcc tgctcgatct gctggccagc ctgctgcacc agcgccgggc cagcggtggc
     5521 ggtcttgccc ttggattcac gcagcagcac ccacggctga taaccggcgc gggtggtgtg
     5581 cttgtccttg cggttggtga agcccgccaa gcggccatag tggcggctgt cggcgctggc
     5641 cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc
     5701 tgcggcgtcg gccaccttga cccatgcctg atagttcttc gggctggttt ccactaccag
     5761 ggcaggctcc cggccctcgg ctttcatgtc atccaggtca aactcgctga ggtcgtccac
     5821 cagcaccaga ccatgccgct cctgctcggc gggcctgata tacacgtcat tgccctgggc
     5881 attcatccgc ttgagccatg gcgtgttctg gagcacttcg gcggctgacc attcccggtt
     5941 catcatctgg ccggtggtgg cgtccctgac gccgatatcg aagcgctcac agcccatggc
     6001 cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc ttcatcgggc caccaagcga
     6061 ttcccacaca ttatacgagc cggaagcata aagtgtagct agatccgaag gatgagccgg
     6121 gctgaatgat cgaccgagac aggccctgcg gggctgcaca cgcgccccca cccttcgggt
     6181 agggggaaag gccgctaaag cggctaaaag cgctccagcg tatttctgcg gggtttggtg
     6241 tggggtttag cgggctttgc ccgcctttcc ccctgccgcg cagcggtggg gcggtgtgta
     6301 gcctagcgca gcgaatagac cagctatccg gcctctggcc gggcatattg ggcaagggca
     6361 gcagcgcccc acaagggcgc tgataaccgc gcctagtgga ttattcttag ataatcatgg
     6421 atggattttt ccaacacccc gccagccccc gcccctgctg ggtttgcagg tttgggggcg
     6481 tgacagttat tgcaggggtt cgtgacagtt attgcagggg ggcgtgacag ttattgcagg
     6541 ggttcgtgac agttagggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga
     6601 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
     6661 gttttatttg atgcctttaa ttaaacgttc gtaatcaagc cacttccttt ttgcattgac
     6721 gcagggtgtc ggaaggcaac tcgccgaacg cgctcctata gttttcagcg aagcgtccca
     6781 aatgtaagaa gccgtagtct agggctatct cagttatact acgcacattg gcactgggat
     6841 cgttcaagca ggcgcggatg ctttcgagct tgcggttgcg gatgtagttc ttcggcgtgg
     6901 tgccggcgtg cttctcgaac aaattgtaga gcgagcgtgg actcatcatc gccagctccg
     6961 ctaaccgctc aaggctgata ttccgtttga gattctcctc aatgaattga acgactcgct
     7021 cgaaagacgg gttacctttg ctgaaaattt cacggctgac attgctgccc agcatttcga
     7081 gcagcttgga agcgatgatc cccgcatagt gctcttggac ccgaggcatc gactttgtat
     7141 gttccgcttc gtcacaaact aacccgagta gattgataaa gccatcgagt tgctggagat
     7201 tgtgtcgcgc ggcgaaacgg ataccctccc tcggcttgtg ccaattgttg tcactgcacg
     7261 cccgatcaag gaccactgag ggcaatttaa cgataaattt ctcgcaatct tctgaatagg
     7321 tcaggtcggc ttggtcatcc ggattgagca gcaatagttc gcccggcgca aaatagtgct
     7381 cctggccatg gccacgccac aggcaatggc ctttgagtat tatttgcaga tgataacagg
     7441 tctctaatcc aggcgagatt accctcacgc taccgccgta gctgattcga cacagatcga
     7501 ggcatccgaa gattctgtgg tgcagcctgc ctgccgggcg cccgcccttg ggcaggcgaa
     7561 tagagtgcgt accgacatac tggttaacat aatcggagac tgcatagggc tcggcgtgga
     7621 cgaagatctg acttttctcg ttcaataagc aaaaatccat agttcacggt tctcttattt
     7681 taatgtgggc tgcttggtgt gatgtagaaa ggcgccaagt cgatgaaaat gcatctcgac
     7741 gtgatgcgta tacgggttac ccccattgcc acgttgcgcc atcctttttg caatcagtga
     7801 ccacttttcc aagcaaaaat aacgccaagc agaacgaaga cgttcttttt aagaagcgag
     7861 aacaccagaa gttcgtgctg tcggggcatg gggcgacgaa ttggcggata aaggggatct
     7921 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     7981 ctttattcac attcttgccc gcctgatgaa tgctcatccg taattacgta tggcaatgaa
     8041 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     8101 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     8161 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     8221 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     8281 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgcatg ggaattagct
     8341 tgatctgacc aacgaccggt agcggagcta tccaacggcg gtataccagg aaaacacaca
     8401 gcaggtacat cagaacagta ccatgactga agaacaaata gttttttcct gatccataaa
     8461 gcagaacggc ctgctccatg acaaatctgg ctccccaact aatgccccat gcagccagca
     8521 taaccagcat aaagtgcagt gtccggtttg atagggataa gtccagcctt gcaagaagcg
     8581 gatacaggag tgcaaaaaat ggctatctct agtaaggcct accccttagg ctttatgcaa
     8641 cctctaggat ccgaattcga aggagatata catatgacaa aaacatctaa acttgacgca
     8701 cttagggctg ctacttcacg tgaagacttg gctaaaattt tagatattaa gttggtattt
     8761 ttaactaacg ttctatatag aatcggctcg gataatcaat acactcaatt tacaataccg
     8821 aagaaaggaa aaggggtaag gactatttct gcacctacag accggttgaa ggacatccaa
     8881 cgaagaatat gtgacttact ttctgattgt agagatgaga tctttgctat aaggaaaatt
     8941 agtaacaact attcctttgg ttttgagagg ggaaaatcaa taatcctaaa tgcttataag
     9001 catagaggca aacaaataat attaaatata gatcttaagg atttttttga aagctttaat
     9061 tttggacgag ttagaggata ttttctttcc aatcaggatt ttttattaaa tcctgtggtg
     9121 gcaacgacac ttgcaaaagc tgcatgctat aatggaaccc tcccccaagg aagtccatgt
     9181 tctcctatta tctcaaatct aatttgcaat attatggata tgagattagc taagctggct
     9241 aaaaaatatg gatgtactta tagcagatat gctgatgata taacaatttc tacaaataaa
     9301 aatacatttc cgttagaaat ggctactgtg caacctgaag gggttgtttt gggaaaagtt
     9361 ttggtaaaag aaatagaaaa ctctggattc gaaataaatg attcaaagac taggcttacg
     9421 tataagacat caaggcaaga agtaacggga cttacagtta acagaatcgt taatattgat
     9481 agatgttatt ataaaaaaac tcgggcgttg gcacatgctt tgtatcgtac aggtgaatat
     9541 aaagtgccag atgaaaatgg tgttttagtt tcaggaggtc tggataaact tgaggggatg
     9601 tttggtttta ttgatcaagt tgataagttt aacaatataa agaaaaaact gaacaagcaa
     9661 cctgatagat atgtattgac taatgcgact ttgcatggtt ttaaattaaa gttgaatgcg
     9721 cgagaaaaag catatagtaa atttatttac tataaatttt ttcatggcaa cacctgtcct
     9781 acgataatta cagaagggaa gactgatcgg atatatttga aggctgcttt gcattctttg
     9841 gagacatcat atcctgagtt gtttagagaa aaaacagata gtaaaaagaa agaaataaat
     9901 cttaatatat ttaaatctaa tgaaaagacc aaatattttt tagatctttc tgggggaact
     9961 gcagatctga aaaaatttgt agagcgttat aaaaataatt atgcttctta ttatggttct
    10021 gttccaaaac agccagtgat tatggttctt gataatgata caggtccaag cgatttactt
    10081 aattttctgc gcaataaagt taaaagctgc ccagacgatg taactgaaat gagaaagatg
    10141 aaatatattc atgttttcta taatttatat atagttctca caccattgag tccttccggc
    10201 gaacaaactt caatggagga tcttttccct aaagatattt tagatatcaa gattgatggt
    10261 aagaaattca acaaaaataa tgatggagac tcaaaaacgg aatatgggaa gcatattttt
    10321 tccatgaggg ttgttagaga taaaaagcgg aaaatagatt ttaaggcatt ttgttgtatt
    10381 tttgatgcta taaaagatat aaaggaacat tataaattaa tgttaaatag ctaataccgt
    10441 tcatatgagg ctagagcagg tgatacgcat gtaggcagat ttgttggttg tgaatcgcaa
    10501 ccagtggcct taatggcagg aggaatcgcc tccctaaaat ccttgaagtg aagagcgagt
    10561 gtgagccgtt gtagctt
//
