LOCUS       sequence-491582-       12612 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  Lentiviral constitutive expression construct for CIC::DUX4 (pLEX_305
            backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12612)
  TITLE     CIC-family fusion oncoprotein expression constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX_305_CIC-DUX4
FEATURES             Location/Qualifiers
     source          1..12612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2823..3033
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2845..3041
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2892..3027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3073..3675
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3073..3092)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3569..3589
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3718..3835
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3893..4393
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4312..4330
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4414..4438
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6445..6454
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7482..7499)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(9446..9470)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9522..10110
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9575..9595)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9993..10004)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10182..10415
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10493..10614
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10530..10549)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10584..10603
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10654..10789
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10809..10828)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10810..10828)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10838..10855)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10838..10854)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10847..10869)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10996..11451
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11083..11102)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11293..11314
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11477..11581
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11582..11650,11651..12442)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11800..11819)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2821 cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc
     2881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2941 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca
     3061 ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     3121 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     3181 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     3241 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg
     3301 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     3361 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     3421 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     3481 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     3541 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     3601 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     3661 cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt
     3721 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     3781 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     3841 ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca ggttgcgcct
     3901 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     3961 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4021 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4081 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4141 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4201 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     4261 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4321 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4381 cctctctccc caggggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctta
     4441 atgtattcgg cccacaggcc cctgatgccc gcgtccagcg cggcctcccg tggcctcggc
     4501 atgttcgtgt ggacgaatgt ggaacctcgc tctgtggctg tgttcccttg gcactcctta
     4561 gtccccttcc tggcacccag ccagcctgac ccctccgtgc agccgagcga ggcccagcaa
     4621 cctgccagcc acccagtggc ctccaaccag agcaaagaac ctgctgagtc ggcagctgtt
     4681 gctcatgaac ggccaccagg tgggacaggg agtgctgacc ctgagcggcc ccctggagcc
     4741 acatgccctg agagcccagg acccggaccc ccacaccctt tgggggtggt ggaatctggt
     4801 aagggtccgc ctcccaccac ggaggaggag gcctccggcc ccccaggaga gccccggctg
     4861 gacagtgaga cagagagtga ccatgatgat gccttcctct ccatcatgtc tcctgagatc
     4921 cagttgcctc taccgcccgg aaaacgtcgg acccagtccc tcagtgccct acccaaggaa
     4981 cgggactcat cttctgagaa ggatggacgc agccccaaca agcgggagaa ggaccacatc
     5041 cggcggccca tgaatgcctt catgatcttc agcaagcggc accgggccct ggtccaccag
     5101 cgtcatccca accaggacaa ccggaccgtc agcaagatcc tgggcgagtg gtggtatgcc
     5161 ctggggccca aggagaagca gaagtaccac gacctggcct tccaggtgaa ggaggcccac
     5221 ttcaaggccc acccagattg gaagtggtgt aacaaggacc gaaagaagtc cagctcagag
     5281 gccaagccca cgagcctggg gctggcagga gggcacaagg agacgcggga gcggagcatg
     5341 tcggagacgg gcactgctgc tgcccctggg gtgtcctctg agctcctgtc cgttgcagcc
     5401 cagacactcc tgagctcaga caccaaggct ccggggagca gctcctgtgg ggcagaacgg
     5461 ctacacacag ttgggggacc tggctcagcc cggccccgag ctttctccca cagcggggta
     5521 cacagcctgg acggcggaga agtagacagt caggcgctac aggaactgac gcagatggtg
     5581 tctggccctg catcgtactc tggcccaaag ccttctaccc agtatggagc tccaggaccc
     5641 tttgcagccc ctggtgaggg aggtgccttg gcggccactg ggcggccccc gctgctgccc
     5701 acccgagctt ctcgttctca gcgtgcggcc agtgaggaca tgacgagtga tgaggagcgc
     5761 atggtcatct gtgaggagga aggggatgat gatgtcattg ctgacgatgg cttcggcacc
     5821 actgacattg atctcaagtg caaggagcgg gtgaccgaca gcgagagtgg ggacagctct
     5881 ggggaggacc cagagggcaa caagggcttt ggtcggaagg tgttttcacc tgtgatccgt
     5941 tcctccttta cccactgccg ccccccactg gaccctgagc ccccagggcc cccggatcct
     6001 cctgtagcct ttggcaaagg ctatggttcc gccccatcct cctctgcgtc ctcgcctgct
     6061 tcctcctcag cctcggcagc cacctccttc tcactgggct caggaacctt caaggcccag
     6121 gagtctggtc agggcagcac agcgggcccc ctacggcccc caccccctgg ggctgggggt
     6181 ccagcgacac cttccaaggc aacccggttc ctcccaatgg atcctgccac cttccggcgc
     6241 aagagacccg aaagtgtggg tggcctggag ccaccaggcc cctcagtcat cgcggcccct
     6301 cccagcggag gaggaaacat cctgcagaca ctggtgctgc ccccaaacaa ggaggagcaa
     6361 gagggcggcg gagccagagt gccctccgcc cccgccccat cactggccta tggggcccca
     6421 gcagctcccc tgtcccgtcc tgccgccacc atggtcacca atgtggtgcg gcctgtcagc
     6481 agcactcctg tgcccatcgc ctctaagccc ttccccacct ctggccgggc tgaggcgtct
     6541 ccaaatgaca cagcaggtgc caggactgaa atgggcactg ggtctcgggt gcctgggggc
     6601 tccccgctgg gtgtcagctt agtgtattcg gacaagaagt cggcagcagc cacctcacca
     6661 gccccacact tggtggctgg acccctgctg ggcactgtgg ggaaggcgcc tgccactgtc
     6721 actaacctac tggtgggcac cccggggtat ggggcccctg cgccccctgc tgtccagttc
     6781 attgcccagg gggcccctgg tggtgggacc actgcgggct caggagcagg tgctgggagt
     6841 ggccccaatg ggccagtacc cctgggcatc ctgcaaccag gtgccctggg caaggctggg
     6901 ggaatcaccc aggtacagta catcctgccc acgctgcccc agcagcttca ggtggcacct
     6961 gccccagcac cagcccctgg gaccaaggca gcggctccca gcggccctgc acccaccacc
     7021 agcatccgtt tcaccctccc accgggcact tccaccaacg gcaaagtcct ggctgccact
     7081 gcacccactc ctggcatccc catcctgcag tctgtaccct ccgccccacc ccccaaagcc
     7141 cagtcagttt ctcccgtgca ggccccgccc ccgggtggct cagcccagct gctgcctggg
     7201 aaggtcctag tgcctctggc cgcccctagc atgtcagtgc ggggtggagg ggccggccag
     7261 ccactgccac tggtgagccc gcccttctca gtacctgtgc agaatggtgc ccagcccccc
     7321 agcaagatca tccagctgac cccggtgcct gtgagcacac ccagcggcct ggtgccgccc
     7381 ctgagcccag ccacactccc tggacccacc tctcagcctc agaaggtcct gttgccctcc
     7441 tccaccagaa tcacctatgt gcagtcagcg ggcgggcacg cgctgcccct gggtaccagc
     7501 cctgcgtcca gccaggctgg aacagtcacc tcgtacgggc ccacgagctc tgtagctcta
     7561 ggcttcacct cgctggggcc cagcggcccc gccttcgtgc agcccctgct ctcagcaggc
     7621 caagccccac tgctggctcc cggtcaggtg ggcgtgtcac ctgtgcccag tccccagctg
     7681 ccgcctgcct gtgcagcccc cggaggtcct gtcataacag cattttactc tggcagccct
     7741 gcacccacct cctcagcacc cctggcccag ccatcccagg cccccccaag cctggtctac
     7801 actgtggcca ccagcacaac cccacctgca gccaccattc tgcccaaggg cccgccagcc
     7861 cctgccactg ccaccccagc cccgactagc cctttcccca gcgccacagc aggttccatg
     7921 acctacagct tagtggcccc caaggcccag cggcccagcc cgaaggcccc ccagaaagtg
     7981 aaggcagcca tcgccagcat tcccgtgggg tcctttgagg caggtgcctc tgggcggcct
     8041 ggccctgcac cccggcagcc tctggagcct ggcccagtcc gagagccaac tgccccagag
     8101 tctgagcttg aggggcagcc cacaccacca gcccctccac ccctgccaga gacctggact
     8161 cccacggccc ggagcagccc cccactgccc ccacctgctg aggagcggac cagcgccaag
     8221 ggccctgaga ccatggccag caaattcccc agctcatctt cagactggcg cgtccctggg
     8281 cagggcctgg agaatcgtgg ggagcctccc actcctccca gcccggcccc agctccagct
     8341 gtagcccctg gtggcagcag cgagagcagc agtgggcggg cagccgggga caccccggag
     8401 cgcaaggagg cggctggtac tggcaagaag gtgaaggtgc ggcccccgcc cctgaagaag
     8461 acctttgact ctgtggacaa cagggtcctg tcagaagtgg acttcgaaga gcgctttgct
     8521 gagttgcctg agtttcggcc tgaggaggtg ctgccctccc ccaccctgca gtctctggcc
     8581 acctcacccc gggccatcct gggctcttac cgcaagaaga ggaagaactc cacggacctg
     8641 gattcagcac ccgaggaccc cacctcgccc aagcgcaaga tgagaagacg ctccagctgc
     8701 agctcggagc ccaacacccc caagagtgcc aagtgcgagg gggacatctt cacctttgac
     8761 cgtacagaag ccgaggacgt gcttggggag ctagagtatg acaaggtgcc atactcctcc
     8821 ctgcggcgca ccctggacca gcgccgggcc ctggtcatgc agctctttca ggaccatggc
     8881 ttcttcccgt cagcccaggc cacagccgcc ttccaggccc gctatgcaga catctttccc
     8941 tccaaggttt gtctgcagtt gaagatccgt gaggtgcgcc agaagatcat gcaggctgcc
     9001 actcccacgg agcagccccc tggagctgag gctcctctcc ctgtaccgcc ccccactggc
     9061 accgctgctg cccctgcccc cactcccagc cccgcagggg gccctgaccc cacctcaccc
     9121 agctcggact ctgggggtgg accccaagcc ggggcagctc cacctcccca gcccgcgccc
     9181 ccggacgcct ccgcctccgc gcggcagggg cagatgcaag gcatcccggc gccctcccag
     9241 gcgctccagg agccggcgcc ctggtctgca ctcccctgcg gcctgctgct ggatgagctc
     9301 ctggcgagcc cggagtttct gcagcaggcg caacctctcc tagaaacgga ggccccgggg
     9361 gagctggagg cctcggaaga ggccgcctcg ctggaagcac ccctcagcga ggaagaatac
     9421 cgggctctgc tggaggagct ttaaaaccca gctttcttgt acaaagtggt taccggttag
     9481 taatgaacta gttaagtcga caatcaacgc gttaagtcga caatcaacct ctggattaca
     9541 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     9601 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     9661 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     9721 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     9781 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     9841 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     9901 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     9961 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
    10021 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
    10081 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc
    10141 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
    10201 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
    10261 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10321 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10381 cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct
    10441 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
    10501 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10561 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10621 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
    10681 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    10741 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
    10801 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
    10861 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
    10921 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
    10981 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
    11041 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
    11101 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
    11161 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
    11221 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
    11281 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
    11341 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
    11401 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
    11461 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    11521 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    11581 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    11641 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    11701 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    11761 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    11821 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    11881 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    11941 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    12001 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    12061 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    12121 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    12181 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    12241 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    12301 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    12361 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    12421 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    12481 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    12541 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    12601 caaaggatct tc
//
