LOCUS       sequence-491585-       11136 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  Lentiviral constitutive expression construct for HA-tagged 
            ATXN1::DUX4 (pLEX_305 backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11136)
  TITLE     CIC-family fusion oncoprotein expression constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX_305_HA-ATXN1-DUX4
FEATURES             Location/Qualifiers
     source          1..11136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2823..3033
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2845..3041
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2892..3027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3073..3675
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3073..3092)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3569..3589
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3718..3835
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3893..4393
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4312..4330
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4414..4438
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4450..4476
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4456..4482
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4483..4509
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4489..4515
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4516..4542
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4522..4548
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    complement(7970..7994)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8046..8634
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8099..8119)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8517..8528)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8706..8939
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9017..9138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9054..9073)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9108..9127
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9178..9313
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9333..9352)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9334..9352)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9362..9379)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9362..9378)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9371..9393)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9520..9975
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9607..9626)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9817..9838
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10001..10105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10106..10174,10175..10966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10324..10343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2821 cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc
     2881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2941 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca
     3061 ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     3121 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     3181 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     3241 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg
     3301 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     3361 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     3421 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     3481 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     3541 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     3601 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     3661 cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt
     3721 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     3781 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     3841 ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca ggttgcgcct
     3901 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     3961 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4021 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4081 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4141 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4201 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     4261 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4321 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4381 cctctctccc caggggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctta
     4441 atggcaggtt acccatacga cgttcctgac tatgcgtcac tctaccccta tgacgtaccg
     4501 gattatgcat ccctatatcc gtatgatgtt ccagattacg cttctctacg ttcctctaga
     4561 ggcgtccacc atatgttatc acttcgaatt cgatatcgag ctcccaggta ccgatctcga
     4621 gcggccgcca tgaaatccaa ccaagagcgg agcaacgaat gcctgcctcc caagaagcgc
     4681 gagatccccg ccaccagccg gtcctccgag gagaaggccc ctaccctgcc cagcgacaac
     4741 caccgggtgg agggcacagc atggctcccg ggcaaccctg gtggccgggg ccacgggggc
     4801 gggaggcatg ggccggcagg gacctcggtg gagcttggtt tacaacaggg aataggttta
     4861 cacaaagcat tgtccacagg gctggactac tccccgccca gcgctcccag gtctgtcccc
     4921 gtggccacca cgctgcctgc cgcgtacgcc accccgcagc cagggacccc ggtgtccccc
     4981 gtacagtacg ctcacctgcc gcacaccttc cagttcattg ggtcctccca atacagtgga
     5041 acctatgcca gcttcatccc atcacagctg atccccccaa ccgccaaccc cgtcaccagt
     5101 gcagtggcct cggccgcagg ggccaccact ccatcccagc gctcccagct ggaggcctat
     5161 tccactctgc tggccaacat gggcagtctg agccagacgc cgggacacaa ggctgagcag
     5221 cagcacctca gcagggctcc ggggcttatc accccggggt cccccccacc agcccagcag
     5281 aaccagtacg tccacatttc cagttctccg cagaacaccg gccgcaccgc ctctcctccg
     5341 gccatccccg tccacctcca cccccaccag acgatgatcc cacacacgct caccctgggg
     5401 cccccctccc aggtcgtcat gcaatacgcc gactccggca gccactttgt ccctcgggag
     5461 gccaccaaga aagctgagag cagccggctg cagcaggcca tccaggccaa ggaggtcctg
     5521 aacggtgaga tggagaagag ccggcggtac ggggccccgt cctcagccga cctgggcctg
     5581 ggcaaggcag gcggcaagtc ggttcctcac ccgtacgagt ccaggcacgt ggtggtccac
     5641 ccgagcccct cagactacag cagtcgtgat ccttcggggg tccgggcctc tgtgatggtc
     5701 ctgcccaaca gcaacacgcc cgcagctgac ctggaggtgc aacaggccac tcatcgtgaa
     5761 gcctcccctt ctaccctcaa cgacaaaagt ggcctgcatt tagggaagcc tggccaccgg
     5821 tcctacgcgc tctcacccca cacggtcatt cagaccacac acagtgcttc agagccactc
     5881 ccggtgggac tgccagccac ggccttctac gcagggactc aaccccctgt catcggctac
     5941 ctgagcggcc agcagcaagc aatcacctac gccggcagcc tgccccagca cctggtgatc
     6001 cccggcacac agcccctgct catcccggtc ggcagcactg acatggaagc gtcgggggca
     6061 gccccggcca tagtcacgtc atccccccag tttgctgcag tgcctcacac gttcgtcacc
     6121 accgcccttc ccaagagcga gaacttcaac cctgaggccc tggtcaccca ggccgcctac
     6181 ccagccatgg tgcaggccca gatccacctg cctgtggtgc agtccgtggc ctccccggcg
     6241 gcggctcccc ctacgctgcc tccctacttc atgaaaggct ccatcatcca gttggccaac
     6301 ggggagctaa agaaggtgga agacttaaaa acagaagatt tcatccagag tgcagagata
     6361 agcaacgacc tgaagatcga ctccagcacc gtagagagga ttgaagacag ccatagcccg
     6421 ggcgtggccg tgatacagtt cgccgtcggg gagcaccgag cccaggtcag cgttgaagtt
     6481 ttggtagagt atcctttttt tgtgtttgga cagggctggt catcctgctg tccggagaga
     6541 accagccagc tctttgattt gccgtgttcc aaactctcag ttggggatgt ctgcatctcg
     6601 cttaccctca agaacctgaa gaacggctct gttaaaaagg gccagcccgt ggatcccgcc
     6661 agcgtcctgc tgaagcactc aaaggccgac ggcctggcgg gcagcagaca caggtatgcc
     6721 gagcaggaaa acggaatcaa ccaggggagt gcccagatgc tctctgagaa tggcgaactg
     6781 aagtttccag agaaaatggg attgcctgca gcgcccttcc tcaccaaaat agaacccagc
     6841 aagcccgcgg caacgaggaa gaggaggtgg tcggcgccag agagccgcaa actggagaag
     6901 tcagaagacg aaccactgag gcagcaccgg cgggaatctc ggccctggcc cgggagacgc
     6961 ggcccgccag aaggccggcg aaagcggacc gccgtcaccg gatcccagac cgccctgctc
     7021 ctccgagcct ttgagaagga tcgctttcca ggcatcgccg cccgggagga gctggccaga
     7081 gagacgggcc tcccggagtc caggattcag atctggtttc agaatcgaag ggccaggcac
     7141 ccgggacagg gtggcagggc gcccgcgcag gcaggcggcc tgtgcagcgc ggcccccggc
     7201 gggggtcacc ctgctccctc gtgggtcgcc ttcgcccaca ccggcgcgtg gggaacgggg
     7261 cttcccgcac cccacgtgcc ctgcgcgcct ggggctctcc cacagggggc tttcgtgagc
     7321 caggcagcga gggccgcccc cgcgctgcag cccagccagg ccgcgccggc agaggggatc
     7381 tcccaacctg ccccggcgcg cggggatttc gcctacgccg ccccggctcc tccggacggg
     7441 gcgctctccc accctcaggc tcctcggtgg cctccgcacc cgggcaaaag ccgggaggac
     7501 cgggacccgc agcgcgacgg cctgccgggc ccctgcgcgg tggcacagcc tgggcccgct
     7561 caagcggggc cgcagggcca aggggtgctt gcgccaccca cgtcccaggg gagtccgtgg
     7621 tggggctggg gccggggtcc ccaggtcgcc ggggcggcgt gggaacccca agccggggca
     7681 gctccacctc cccagcccgc gcccccggac gcctccgcct ccgcgcggca ggggcagatg
     7741 caaggcatcc cggcgccctc ccaggcgctc caggagccgg cgccctggtc tgcactcccc
     7801 tgcggcctgc tgctggatga gctcctggcg agcccggagt ttctgcagca ggcgcaacct
     7861 ctcctagaaa cggaggcccc gggggagctg gaggcctcgg aagaggccgc ctcgctggaa
     7921 gcacccctca gcgaggaaga ataccgggct ctgctggagg agctttagaa cccagctttc
     7981 ttgtacaaag tggttaccgg ttagtaatga actagttaag tcgacaatca acgcgttaag
     8041 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     8101 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     8161 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     8221 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     8281 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     8341 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     8401 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     8461 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     8521 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     8581 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     8641 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8701 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8761 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8821 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8881 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8941 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     9001 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     9061 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9121 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     9181 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9241 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9301 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     9361 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     9421 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     9481 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     9541 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     9601 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     9661 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     9721 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     9781 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     9841 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     9901 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     9961 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    10021 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    10081 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
    10141 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
    10201 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
    10261 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
    10321 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
    10381 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
    10441 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    10501 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    10561 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    10621 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    10681 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    10741 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    10801 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    10861 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    10921 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    10981 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    11041 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    11101 agcgtcagac cccgtagaaa agatcaaagg atcttc
//
