LOCUS       sequence-491669-       11488 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  Expresses a synNotch receptor binding to CSPG5 Neuroglycan.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Simic MS, Watchmaker PB, Gupta S, Wang Y, Sagan SA, Duecker J, 
            Shepherd C, Diebold D, Pineo-Cavanaugh P, Haegelin J, Zhu R, Ng B, 
            Yu W, Tonai Y, Cardarelli L, Reddy NR, Sidhu SS, Troyanskaya O, 
            Hauser SL, Wilson MR, Zamvil SS, Okada H, Lim WA
  TITLE     Programming tissue-sensing T cells that deliver therapies to the 
            brain.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4237. doi: 
            10.1126/science.adl4237. Epub 2024 Dec 6.
  PUBMED    39636984
REFERENCE   2  (bases 1 to 11488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4237. doi"; pages: " 
            10.1126/science.adl4237. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR_PGK_ CSPG5 Neuroglycan 12960 VLVH synNotch
FEATURES             Location/Qualifiers
     source          1..11488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(326..370)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    555..788
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1285..1410
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1457..2090
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2258..2392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2282..2301)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2336..2355
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2817..2837)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          2967..3032
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(4166..4495)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4180..4315
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4480..4500
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(4570..4587)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4741..5329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4821..4840)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5500..6291,6292..6360))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6123..6142
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6361..6465)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6533..6551
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6589..6611)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6711..6730
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6944..6963
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        7253..7271
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7253..7270
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             7296..7529
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     7619..7635
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7620..7636
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7639..8227
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7745..7756
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8154..8174
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8346..8495)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(10707..10736)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      10796..10805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10853..11352)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10901..10920)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     11294..11317
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    join(11412..11488,1..41)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
       61 tttggcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc
      121 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
      181 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
      241 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
      301 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
      361 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
      421 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
      481 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
      541 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
      601 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
      661 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg
      721 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca
      781 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg
      841 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat
      901 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccggccgctt gctgtgcggt
      961 ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     1021 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     1081 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     1141 tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat
     1201 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     1261 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
     1321 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
     1381 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
     1441 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
     1501 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     1561 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     1621 tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc
     1681 tcgccactcc ccagtcccgc ccaggccacg cctccctgga aagtccccag cggaaagtcc
     1741 cttgtagcaa gctcgatatc agcagttctt gaagtactcc ggatgcagct ctcgggccac
     1801 gtgatgaaat gctaggcggc tgtcaaacct ccactctaac acttctctct ccgggtcatc
     1861 catcccatgc aggctcacag ggtgtaacaa gctggtgttc tctcctttat tggcctcttc
     1921 taccttatct ggctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct
     1981 gacccctggc cctggtgtgt agttctgcta atcagggaag tagccttgtg tgtggtagat
     2041 ccacagatca aggatatctt gtcttctttg ggagtgaatt agcccttcca actactaagt
     2101 ttgtagtaca tatttaacaa atacaatttc tttaaaatga aaataattca gaggaatcac
     2161 aggtttagag taaatgaaac cacaggtaat tggcagtggt aatagggtat ggggtgggaa
     2221 gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga taagatacat
     2281 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     2341 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     2401 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     2461 gtaaaacctc tacaaatgtg gtatggctga ttatgatctc tagtcaaggc actatacatc
     2521 aaatattcct tattaacccc tttacaaatt aaaaagctaa aggtacacaa tttttgagca
     2581 tagttattaa tagcagacac tctatgcctg tgtggagtaa gaaaaaacag tatgttatga
     2641 ttataactgt tatgcctact tataaaggtt acagaatatt tttccataat tttcttgtat
     2701 agcagtgcag ctttttcctt tgtggtgtaa atagcaaagc aagcaagagt tctattacta
     2761 aacacagcat gactcaaaaa acttagcaat tctgaaggaa agtccttggg gtcttctacc
     2821 tttctcttct tttttggagg agtagaatgt tgagagtcag cagtagcctc atcatcacta
     2881 gatggcattt cttctgagca aaacaggttt tcctcattaa aggcattcca ccactgctcc
     2941 cattcatcag ttccataggt tggaatctaa aatacacaaa caattagaat cagtagttta
     3001 acacattata cacttaaaaa ttttatattt accttagagc tttaaatctc tgtaggtagt
     3061 ttgtccaatt atgtcacacc acagaagtaa ggttccttca caaagatccg gggcccactc
     3121 ataaatccag ttgccgccac ggtagccaat caccgtatcg tataaatcat cgtcggtacg
     3181 ttcggcatcg ctcatcacaa tacgtgcctg gacgtcgagg atttcgcgtg ggtcaatgcc
     3241 gcgccagatc cacatcagac ggttaatcat gcgataccag tgagggatgg ttttaccatc
     3301 aagggccgac tgcacaggcg gttgtgcgcc gtgattaaag cggcggacta gcgtcgaggt
     3361 ttcaggatgt ttaaagcggg gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga
     3421 tgcagcctcc agaatactta ctggaaacta ttgtaacccg cctgaagtta aaaagaacaa
     3481 cgcccggcag tgccaggcgt tgaaaagatt agcgaccgga gattggcggg acgaatacga
     3541 cgcccatatc ccacggctgt tcaatccagg tatcttgcgg gatatcaaca acatagtcat
     3601 caaccagcgg acgaccagcc ggttttgcga agatggtgac aaagtgcgct tttggataca
     3661 tttcacgaat cgcaaccgca gtaccaccgg tatccaccag gtcatcaata acgatgaagc
     3721 cttcgccatc gccttctgcg cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt
     3781 agctggaaat acaaacggta tcgacatgac gaatacccag ttcacgcgcc agtaacgcac
     3841 ccggtacaga ccgccacggc ttacggcaat aatgcctttc cattgttcag aaggcatcag
     3901 tcggcttgcg agtttacgtg catggatctg caacatgtcc caggtgacga tgtatttttc
     3961 gctcatgtga agtgtcccag cctgtttatc tacggcttaa aaagtgttcg aggggaaaat
     4021 aggttgcgcg agattataga gatctggcgc actaaaaacc agtatttcac atgagtccgc
     4081 gtctttttac gcactgcctc tccctgacgc gggataaagt ggtattctca aacatatctc
     4141 gcaagcctgt cttgtgtcca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     4201 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     4261 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     4321 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4381 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4441 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4501 acagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4561 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4621 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4681 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4741 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4801 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4861 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4921 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4981 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5041 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5101 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5161 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5221 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5281 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5341 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5401 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5461 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5521 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5581 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5641 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5701 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5761 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5821 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5881 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5941 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6001 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6061 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6121 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6181 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6241 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6301 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6361 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6421 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6481 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     6541 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     6601 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     6661 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     6721 gcagattgta ctgagagtgc accattcgac gctctccctt atgcgactcc tgcattagga
     6781 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
     6841 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
     6901 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
     6961 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
     7021 ggatctggct agcgatgacc ctgctgattg gttcgctgac catttccggg tgcgggacgg
     7081 cgttaccaga aactcagaag gttcgtccaa ccaaaccgac tctgacggca gtttacgaga
     7141 gagatgatag ggtctgcttc agtaagccag atgctacaca attaggcttg tacatattgt
     7201 cgttagaacg cggctacaat taatacataa ccttatgtat catacacata cgatttaggt
     7261 gacactatag aatacacgga attaattcta gatgctgcta gagattttcc acactgacta
     7321 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     7381 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     7441 ctcccaggct cagatctggt ctaaccagag agacccagta caagcaaaaa gcagatcttg
     7501 tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag
     7561 atctacagct gccttgtaag tcattggtct taaaggtacc gagctcgaat taattccctc
     7621 gaggtcgacg gtatcgatgc ggggaggcgg cccaaaggga gatccgactc gtctgagggc
     7681 gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg
     7741 ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa
     7801 cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg gaattgtcag
     7861 tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg
     7921 tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc
     7981 cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa
     8041 cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga
     8101 aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg
     8161 taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg taatccagag
     8221 gttgattatc gataagcttg atatcaagct tgcatgcctg caggtcgact ctagagtcgc
     8281 ggcctcagtc agtcacgcat gttgcaggtg ggagttgcgg ccgcaagtca aggatctcat
     8341 gatccgagca tgtccaggtc aaagtcatcc agtgcatctg atcccaacat gtcgagatcg
     8401 aaatcgtcca gagcgtcaga tcccagcatg tccagatcaa aatcatccag ggcgtcgctg
     8461 cccagcatat ccaggtcgaa gtcgtccagg gcatcagatc cgccagagcc tccgcttccg
     8521 cctgcagcag cagacactgt cagctgccgc tggcccttgt tgctgctttc ctcgctgctg
     8581 ctggtggcgc tgattctgtg ctgccgcagg gtcaggggca tgtcggtttc cacgctggcc
     8641 agtctgtcgg tcacggcgtc cttgttcacg ttgtcctgca cgaacaggcc ggtcagcagg
     8701 gccttgatgt cctgcaggct gtccatcttc aggatcatgt ccaggtcctc gcgtgggaag
     8761 atcagcagaa acagctgttc cagtctttcc agccggcttt ccacctcggt caggtgggct
     8821 ctggtcaggg ggcttctctt ggtcttgggg ctgtaccggc actcccagtt gttcttcagg
     8881 cacttggcgc acttgggctt ttctttgctg cacttcagtt tcttcagccg gcagatgtca
     8941 caggcctgct cgatgctgct cagcagcttc atccgccggc gcttgcggga cagcagcacc
     9001 ccacagccca caaagaacag gagcacgaag gcggccgctg ccacgtacat gaggtgcagc
     9061 tgcgagggca gcggaggctc caccggctca ctcttcacgg cctcaatctt gtaaggaata
     9121 ttgaggctgc caagtgacgc aagagcacct aggaaggcag ccacatcggt ggcactctgg
     9181 aagcactgcg aggatgactg cacacattgc cggttgtcga tctccaggta gacaatggag
     9241 ccacggatgt ccatggggtc cagctccctg cgctggcgcc caccactggt accaggaagc
     9301 agtgaagagg tggcccaacc cactgtagag cgcttgattg ggtgcttgcg cagctcttcc
     9361 tcgtggccat agtacgggaa gatcatctgc tggccttgcg catcacgctt gaagaccacg
     9421 ttggtgtgca gcacgtggct gagctcccgc agaaagtgga aggagttgtt ccgtagctgg
     9481 tcgggtggaa gcagcaccac cagcaccagg gtgccggctg ccagccgctc gggtacatgc
     9541 tcagcacagt ctaggccatc ccactcacat tcggcactgt tacagccctg gtcgcagtgg
     9601 ccatcactga agtggtcctt gcagtactgg tcatacaggg ggttgcactg tccctcggtg
     9661 agctggcagt cgaagccatc aaagaggcag ccggccgagt tgcactggct gtcacagtgg
     9721 ccgtcgctaa aatacttcca gcactgtaga gactgcgtgc agttcttcca ggggtcattg
     9781 aagttgaggg agcagtcgcc accatcccag ccacatgcgt gattattaca ctgcaggttg
     9841 cagaccttat tgcctgcatc cacctggcac tcaggcagct cacaggcctc ctcaatctgc
     9901 ggtgggggaa tgtcgcgccc agcgccacct gtgaagctgt agtccaggat cgaggagacg
     9961 gtgaccaggg ttccttgacc ccagtagtca aaagcagcag agaagtagta gtaactccag
    10021 taccagtagt cgtagaagta gcgagcacaa taatagacgg cagtgtcctc agctcttaag
    10081 ctgttcattt gtaggtaggc tgtgtttttg gatgtgtctg cgcttatagt gaaacggccc
    10141 ttgacgctat cggcataagc agtagcgcct ccagaagcgt aaatatatgc aacccattcc
    10201 aggcccttac ccggggcctg acgcacccag tggatcgaat aagcagcgat atcgaagcca
    10261 gaagctgcac aggacaaacg gagtgagccc cctggctgca ccaggccacc gccagactcc
    10321 accagctgaa cctcggcacc actgctactg ccaccactac taccactagc ggcagtagta
    10381 cctttgatct ccaccttggt accctgtccg aacgtgatca gagaatgcgg gaaagattgc
    10441 tgacagtaat aagttgcgaa gtcttccggc tgcagactgc tgatggtcag agtgaaatcc
    10501 gtcccggaac ggctaccaga gaagcgagaa gggactccag agtagagatc ggatgccgag
    10561 taaatcagaa gcttcggagc ttttcctggt ttctgttgat accaggctac agcgctggac
    10621 acggactgac tggcacggca ggtgatggtg accctatcgc ccacagaggc ggacagggag
    10681 ctcggggact gggtcatctg gatatccaga tcctcttcgc tgataagttt ttgttcaggc
    10741 cttgcagcgt gcagcagcag cgcgagtggg agcaacagtg ccgtcacggg caacgccatg
    10801 gtggcggcga tccactgtcc acctgccttg ctccacttga cgcgtgagaa ttcgaaaggc
    10861 ccggagatga ggaagaggag aacagcgcgg cagacgtgcg cttttgaagc gtgcagaatg
    10921 ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct gagcccgccc ctgagcccgc
    10981 ccccggaccc accccttccc agcctctgag cccagaaagc gaaggagcaa agctgctatt
    11041 ggccgctgcc ccaaaggcct acccgcttcc attgctcagc ggtgctgtcc atctgcacga
    11101 gactagtgag acgtgctact tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg
    11161 gggggaactt cctgactagg ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg
    11221 gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt
    11281 agcgccaagt gcccagcggg gctgctaaag cgcatgctcc agactgcctt gggaaaagcg
    11341 cctcccctac ccaattcgat atcaagctta tcgatccaaa ctggatctct gctgtccctg
    11401 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
    11461 tctgttgcta ttatgtctac tattcttt
//
