LOCUS       sequence-491713-        9330 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  Expresses Venus-NUP107 under EF1 promoter and can be integrated into
            FRT site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Nagashima R, Hibino K, Ashwin SS, Babokhov M, Fujishiro S, Imai R, 
            Nozaki T, Tamura S, Tani T, Kimura H, Shribak M, Kanemaki MT, Sasai 
            M, Maeshima K
  TITLE     Single nucleosome imaging reveals loose genome chromatin networks 
            via active RNA polymerase II.
  JOURNAL   J Cell Biol. 2019 Mar 1. pii: jcb.201811090. doi: 
            10.1083/jcb.201811090.
  PUBMED    30824489
REFERENCE   2  (bases 1 to 9330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2019 Mar 1. pii: jcb.201811090. doi: 10.1083/jcb.201811090."
COMMENT     Sequence Label: pEF-1-alpha-Venus-Nup107-FRT
FEATURES             Location/Qualifiers
     source          1..9330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    69..116
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             124..1146
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             124..1146
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    1276..1397
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1313..1332)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1367..1386
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1446..1462)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1446..1462)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1459..1481)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1470..1486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1494..1500,1501..1518,1519..1524))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1539..1560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1677..1694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1848..2436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1928..1947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2607..3398,3399..3467))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3230..3249
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3468..3572)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3647..3666)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3954..5132
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4185..5123
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5080..5100
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     5149..5168
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5149..5167
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(5252..5254,5255..5257,5258..5968)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5297..5318)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5558..5577)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5905..5926
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8806..8847
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(8885..8902)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8891..9115
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9248..9267)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
       61 cgtacctaga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttccttg
      121 gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
      181 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
      241 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
      301 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
      361 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
      421 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
      481 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
      541 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
      601 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
      661 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
      721 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
      781 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
      841 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
      901 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
      961 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     1021 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     1081 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     1141 gaatagcacg tactacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     1201 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     1261 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1321 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1381 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     1441 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1501 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1561 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1621 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1681 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1741 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1801 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1861 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1921 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1981 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2041 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2101 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2161 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2221 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2281 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2341 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2401 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2461 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2521 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2581 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2641 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2701 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2761 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2821 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2881 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2941 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3001 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3061 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3121 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3181 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3241 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3301 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3361 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3421 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3481 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3541 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3601 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     3661 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     3721 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     3781 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     3841 attgattatt gactaggctt ttgcaaaaag ctttgcaaag atggataaag ttttaaacag
     3901 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
     3961 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     4021 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     4081 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     4141 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     4201 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     4261 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     4321 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     4381 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     4441 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     4501 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     4561 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     4621 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     4681 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     4741 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     4801 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     4861 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     4921 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     4981 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     5041 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     5101 aaagtttttt tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca
     5161 ctatagggag acccaagctg gctaggtaag cttggtaccg agctcggatc cactagtcca
     5221 gtgtggtgga attctgcaga tctagctggc gatggtgagc aagggcgagg agctgttcac
     5281 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     5341 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac
     5401 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca
     5461 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5521 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     5581 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     5641 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     5701 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     5761 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     5821 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     5881 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     5941 cactctcggc atggacgagc tgtacaagct cgagatggac aggagtggct ttggagagat
     6001 atcatcccct gtaatccggg aggcagaggt gacacggact gcacggaaac agagtgctca
     6061 gaaaagagtt ttacttcagg catctcaaga tgaaaatttt ggtaatacta caccaagaaa
     6121 ccaggttatc cctcgaactc ctagctcatt tcgacagcct tttaccccaa caagccgaag
     6181 cttactaagg cagccagata tttcctgcat tcttggaaca ggagggaagt cgccccgact
     6241 tacgcagtct tcagggttct ttggaaatct ctccatggtt actaatctgg atgacagtaa
     6301 ctgggcagct gcattttcat cacagcgttc cgggctgttc acaaacacag agccccacag
     6361 tataacagaa gatgtaacta tcagtgctgt tatgttacgt gaggatgatc ctggagaagc
     6421 tgcatccatg agtatgtttt ctgatttcct gcagtctttt ctgaagcact cttcgagtac
     6481 agtttttgat cttgtggaag agtatgaaaa catctgtggt agtcaggtga atatactgag
     6541 taaaatagtg agtcgagcaa cacctggact tcaaaaattt tcaaaaacag ccagtatgct
     6601 ctggcttctt caacaggaga tggtcacatg gaggctgctg gcttctttgt atagagacag
     6661 aatacagtct gcattagaag aggaaagcgt attcgcagtt actgctgtta atgccagtga
     6721 aaaaacagtt gtggaagcgt tatttcagag ggattcactt gttcgacaaa gtcagctggt
     6781 ggtagattgg ttagagagta ttgccaaaga tgaaattgga gaattttctg ataatattga
     6841 gttttatgca aaatcagtat attgggaaaa tactctgcat accttaaaac aacggcagct
     6901 gacttcttac gttggaagtg ttcgtccgct tgtcactgaa ttggaccctg atgctcccat
     6961 aagacagaaa atgccccttg atgatctgga tagagaagat gaagttagat tactcaaata
     7021 tctctttact ctaatccgtg ctggaatgac agaagaggca caacgactct gtaaacgctg
     7081 tggtcaagca tggagagctg caacacttga aggctggaaa ctgtaccatg accctaatgt
     7141 taatggagga acagaattag aacctgttga agggaatcca tatagacgca tttggaaaat
     7201 aagttgctgg agaatggcag aagatgagct ttttaataga tacgagagag caatttatgc
     7261 agctttaagt gggaatctta agcagctgct tcctgtctgt gacacctggg aagacacagt
     7321 ttgggcctac ttccgggtga tggtggacag tctggtagaa caggagatcc agacatcagt
     7381 agcaactctg gatgaaactg aagaactccc tagagaatat ctgggagcaa actggacgtt
     7441 agaaaaggtt tttgaggaac ttcaagctac tgacaaaaag agagttctgg aagagaatca
     7501 agaacattat catatagttc aaaagtttct tatcctggga gacattgatg gtttgatgga
     7561 tgagtttagc aaatggcttt ccaaaagcag aaacaatcta cctggacacc tgcttcgctt
     7621 tatgacccac cttattttgt ttttccgtac tctgggacta cagaccaagg aggaagtttc
     7681 tattgaagtt ttaaagacat acatacagct tttaataaga gagaaacata caaatcttat
     7741 agcattttat acctgtcatt tgcctcaaga cctagctgtt gcccagtatg cattattttt
     7801 ggaaagtgtt acagaatttg aacagcgcca ccattgcctg gagttggcta aagaagcaga
     7861 tttggatgtt gcaacaataa caaaaactgt agttgagaat attcgaaaga aagataatgg
     7921 tgaatttagt catcatgacc tggccccagc cctagatact ggcactactg aggaggatcg
     7981 tttaaaaatt gatgtaattg actggttggt atttgaccca gcgcagaggg cagaagcact
     8041 gaaacagggc aatgcaatta tgagaaaatt cttggcatca aaaaagcacg aagctgcaaa
     8101 agaagtattt gtgaaaattc ctcaggattc tatagcagaa atctataatc agtgcgagga
     8161 acaaggaatg gaaagtccac ttcctgctga agatgataat gctatccgag aacatttgtg
     8221 catcagagct tatttggaag cccatgaaac ctttaatgag tggtttaagc atatgaattc
     8281 agttccacaa aaacctgctt tgatacctca accaactttt actgagaaag tggctcatga
     8341 acacaaagaa aagaaatatg aaatggattt tggtatttgg aaagggcatt tggatgccct
     8401 aactgctgat gtgaaggaga aaatgtataa cgtcttgttg tttgttgatg gagggtggat
     8461 ggtggatgtt agagaggatg ccaaagaaga ccatgaaaga acacatcaaa tggtcttact
     8521 gagaaagctt tgtctgccaa tgttgtgttt tctgcttcat acgatattgc acagtactgg
     8581 tcagtatcag gaatgcctac agttagcaga tatggtatcc tctgagcgcc acaaactgta
     8641 cctggtattt tctaaggaag agctaaggaa gttgctgcag aagctcagag agtcctctct
     8701 aatgctccta gaccagggac ttgacccatt agggtatgaa attcagttat agggatcatc
     8761 cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct
     8821 aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagttta aacccgctga
     8881 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     8941 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     9001 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     9061 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     9121 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     9181 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9241 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     9301 caagctctaa atcgggggct ccctttaggg
//
