LOCUS       sequence-491723-       11696 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  Express Ymd8p membrane protein cargo, peroxisomal Ynd1p truncation, 
            and Pex22 peroxisomal chauffeur.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11696)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 11696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKS1335
FEATURES             Location/Qualifiers
     source          1..11696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5666..5853
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5721..5745)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        6197..6610
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6744..7451
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     CDS             7969..8772
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(9414..10229)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9414..10229)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10137..10156
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10221..10240
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10454..11042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10943..10962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgttgat aggtcaagat caatgtaaac aattactttg
       61 ttatgtagag tttttttagc tacctatatt ccaccataac atcaatcatg cggttgctgg
      121 tgtatttacc aataatgttt aatgtatata tatatatata tatatggggc cgtatactta
      181 catatagtag atgtcaagcg taggcgcttc ccctgccggc tgtgagggcg ccataaccaa
      241 ggtatctata gaccgccaat cagcaaacta cctccgtaca ttcatgttgc acccacacat
      301 ttatacaccc agaccgcgac aaattaccca taaggttgtt tgtgacggcg tcgtacaaga
      361 gaacgtggga actttttagg ctcaccaaaa aagaaagaaa aaatacgagt tgctgacaga
      421 agcctcaaga aaaaaaaaat tcttcttcga ctatgctgga ggcagagatg atcgagccgg
      481 tagttaacta tatatagcta aattggttcc atcaccttct tttctggtgt cgctccttct
      541 agtgctattt ctggcttttc ctattttttt ttttccattt ttctttctct ctttctaata
      601 tataaattct cttgcatttt ctatttttct ctctatctat tctacttgtt tattcccttc
      661 aaggtttttt tttaaggagt acttgttttt agaatatacg gtcaacgaac tataattaac
      721 taaacagatc tatgaatagg actgtctttt tggcatttgt atttggatgg tatttctgtt
      781 caatagctct ctccatatac aatagatgga tgttcgatcc gaaggacgga ttgggtattg
      841 ggtatcccgt gttggtgaca acattccatc aggctacttt atggttacta tccggcatat
      901 acattaagct caggcataag ccagtgaaaa acgttttaag aaagaataat ggttttaact
      961 ggagtttttt tctaaaattt ttactcccta cggcagtcgc ttctgcaggt gatatcgggc
     1021 tcagtaacgt ttccttccag tatgttccct tgacaattta tacaattatc aaatcttcga
     1081 gcatagcttt tgtcctttta ttcggctgta tcttcaagct agaaaagttt cattggaagt
     1141 tggcactttc agtaataatt atgtttgttg gtgtagcatt aatggttttc aaaccaagcg
     1201 attccaccag taccaaaaac gaccaagcct tggtcatttt tggcagcttt cttgtcctag
     1261 caagtagctg tctttctggg ttaaggtggg tttatacgca gttgatgctt aggaacaacc
     1321 caattcaaac taacaccgct gcagcagtag aggaatctga tggtgccctt ttcacggaaa
     1381 atgaagataa tgtcgataat gaacctgttg ttaacctcgc caataacaaa atgctggaga
     1441 attttggaga atccaagcct cacccaattc atacaatcca ccagttagct cctataatgg
     1501 gcattactct gttgcttacc tcgctacttg tggaaaaacc atttcctggt atattcagct
     1561 ctagcatatt taggcttgat acgagcaatg gcggtgtcgg cacggaaact accgtcctat
     1621 ccattgtgag gggtattgtt ttgttaattt tacctggttt tgcagtcttt ttgttgacaa
     1681 tttgtgagtt tagtattttg gagcaaacgc ccgtgctgac cgtatctatt gtgggtatag
     1741 tgaaggaact tttgacggta atatttggta taattattct ttctgaaaga ttaagtggtt
     1801 tttacaattg gttaggaatg cttattatta tggcagatgt atgctattat aattacttca
     1861 gatataagca agatctatta caaaaatatc attcagtttc gacacaagat aatcgtaacg
     1921 agttaaaggg atttcaagac ttcgaacagt taggaagcaa gaaaatcgca ccatattcta
     1981 ttagtgtcga cctaacaaat caagagtatg agcttgatat gattgctcaa aacgttagtc
     2041 gttcatccca gcaggtcggt tctgtttcta aaggtgaaga attattcact ggtgttgtcc
     2101 caattttggt tgaattagat ggtgatgtta atggtcacaa attttctgtc tccggtgaag
     2161 gtgaaggtga tgctacttac ggtaaattga ccttaaaatt tatttgtact actggtaaat
     2221 tgccagttcc atggccaacc ttagtcacta ctttatcttg gggtgttcaa tgttttgcaa
     2281 gatacccaga tcatatgaaa caacatgact ttttcaagtc tgccatgcca gaaggttatg
     2341 ttcaagaaag aactattttt ttcaaagatg acggtaacta caagaccaga gctgaagtca
     2401 agtttgaagg tgatacctta gttaatagaa tcgaattaaa aggtattgat tttaaagaag
     2461 atggtaacat tttaggtcac aaattggaat acaattattt ctctgacaat gtttacatca
     2521 ctgctgacaa acaaaagaat ggtatcaaag ctaacttcaa aattagacac aacattgaag
     2581 atggtggtgt tcaattagct gaccattatc aacaaaatac tccaattggt gatggtccag
     2641 tcttgttacc agacaaccat tacttatcca ctcaatctaa gttatccaaa gatccaaacg
     2701 aaaagaggga ccacatggtc ttgttagaat ttgttactgc tgctggtatt accttgggta
     2761 tggatgaatt gtacaaagga tccgcagagt atgtgcgggc cctgtttgac tttaatggga
     2821 atgatgaaga agaccttccc tttaagaaag gagacatcct gagaatccgg gataagcctg
     2881 aagagcagtg gtggaatgca gaggacagcg aaggaaagag ggggatgatt cctgtccctt
     2941 acgtggagaa gtatggatct gggagcggat ctggaggcag catggcggaa tatgtacgag
     3001 ctctttttga ttttaatggc aatgatgagg aggatctccc ttttaaaaag ggcgatattc
     3061 tgcgcattcg tgataagcca gaggaacaat ggtggaacgc cgaggatagt gaggggaaac
     3121 gcggcatgat tccggtgccg tatgtagaga agtactaatg gcgccaattg gtgcggcaat
     3181 tgataataac gaaaatgtct tttaatgatc tgggtataat gaggaatttt ccgaacgttt
     3241 ttactttata tatatatata catgtaacat atattctata cgctatagag aaaggaaatt
     3301 tttcaattaa aaaaaaaata gagaaagagt ttcacttctt gattatcgct aacactaatg
     3361 gttgaagtac tgctacttta attttatgct gccaaaccag atgtcaacac agctacaacg
     3421 tatatattaa atttgctctt gttctgtact ttcctaattc ttatgtaaaa agacaagaat
     3481 ttatgatact atttaataac aaaaaactac ctaagaaaag catcatgcag tcgaaattga
     3541 aatcgaaaag taaaacttta acggaacatg tttgaaattc taagaaagca tacatcttca
     3601 tcccttatat atagagttat gtttgatatt agtagtcatg ttgtaatctc tggcctaagt
     3661 atacgtaacg aaaatggtag cacgtcgcgt ttatggcccc caggttaatg tgttctctga
     3721 aattcgcatc actttgagaa ataatgggaa caccttacgc gtgagctgtg cccaccgctt
     3781 cgcctaataa agcggtgttc tcaaaatttc tccccgtttt caggatcacg agcgccatct
     3841 agttctggta aaatcgcgct tacaagaaca aagaaaagaa acatcgcgta atgcaacagt
     3901 gagacacttg ccgtcatata taaggttttg gatcagtaac cgttatttga gcataacaca
     3961 ggtttttaaa tatattatta tatatcatgg tatatgtgta aaattttttt gctgactggt
     4021 tttgtttatt tatttagctt tttaaaaatt ttactttctt cttgttaatt ttttctgatt
     4081 gctctatact caaaccaaca acaacttact ctacaactaa gatctatgct catagaaaac
     4141 actaatgatc ggtttggtat cgtcatagat gcagggtctt cgggttcgag aatccatgtg
     4201 tttaagtggc aggacacgga atcgcttctt catgcaacaa accaggactc acagtccata
     4261 ttacaatcag tacctcatat tcatcaagaa aaagactgga cttttaagct gaatccaggc
     4321 ttgtcgagtt ttgaaaaaaa acctcaagat gcgtacaaat ctcacatcaa gccgttacta
     4381 gattttgcta agaatattat ccctgaatca cattggtcga gttgtcccgt ttttattcaa
     4441 gcaaccgcgg gcatgcgcct tttacctcaa gacatacaat cttccatttt ggatggtttg
     4501 tgtcaggggc tcaaacaccc tgcagaattt ttggttgagg attgctcagc acaaattcaa
     4561 gtcattgatg gtgaaaccga aggtttatat ggctggcttg gcttaaacta tctatatgga
     4621 cactttaatg actataatcc agaagtctct gaccatttta catttgggtt tatggacatg
     4681 ggcggtgcct ctactcagat tgcgtttgca ccgcatgatt caggagaaat agctagacat
     4741 agagatgaca tcgccaccat cttcttgagg agtgttaacg gagatttgca gaaatgggac
     4801 gtttttgtta gtacgtggtt agggtttggt gccaatcagg ctagaagaag gtacttggct
     4861 cagttgatca atacccttcc agaaaacaca aatgattacg aaaatgatga cttctctaca
     4921 aggaacttga atgatccatg tatgccgcga ggaagcagca cggattttga atttaaagat
     4981 accatatttc acatcgcggg ttctgggaat tacgaacaat gtacaaaatc tatttatccg
     5041 ttacttttaa agaacatgcc ttgtgatgac gagccgtgct tgtttaatgg tgttcatgct
     5101 cctcgaatag actttgccaa tgacaaattt ataggtactt ctgagtactg gtacactgcc
     5161 aacgacgtat tcaaacttgg gggcgaatac aactttgaca aatttagcaa aagcctaagg
     5221 gagttttgca attccaattg gacgcaaata ttagcgaaca gtgataaagg cgtgtataat
     5281 tctattccgg agaatttttt gaaagatgca tgctttaagg gcaattgggt ccttaatata
     5341 cttcatgaag ggtttgatat gcctcgaata gatgtcgacg cagaaaatgt caatgacagg
     5401 cccttatttc aaagtgtgga aaaagtcgaa gaacgagagt tatcgtggac actaggcaga
     5461 attttacttt atgcctcagg tagcatattg gcaggtaatg atgacttcat ggtgggtatt
     5521 gcgcccagtg aaagaagaac taagctcact ggtaagaaat tcatacctgg caagttactg
     5581 gagtctgatc aactacgcaa gcaaagttcc ggatccggtt cattgggaag aggtagaaga
     5641 tccaaattgt aataactcga gtggcgcgaa tttcttatga tttatgattt ttattattaa
     5701 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
     5761 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca
     5821 tgaggtcgct cttattgacc acacctctac cggcatgccg agcaaatgcc tgcaaatcgc
     5881 tccccatttc gctggatgca cacactggct taagatgaca acgccttgcc aacagggagt
     5941 tcttcagaga catggaggct caaaacgaaa ttattgacag cctagacatc aatagtcata
     6001 caacagaaag cgaccaccca actttggctg ataatagcgt ataaacaatg catactttgt
     6061 acgttcaaaa tacaatgcag tagatatatt tatgcatatt acatataata catatcacat
     6121 aggaagcaac aggcgcgttg gacttttaat tttcgaggac cgcgaatcct tacatcacac
     6181 ccaatccccc acaagtgatc ccccacacac catagcttca aaatgtttct actccttttt
     6241 tactcttcca gattttctcg gactccgcgc atcgccgtac cacttcaaaa cacccaagca
     6301 cagcatacta aatttcccct ctttcttcct ctagggtgtc gttaattacc cgtactaaag
     6361 gtttggaaaa gaaaaaagac accgcctcgt ttctttttct tcgtcgaaaa aggcaataaa
     6421 aatttttatc acgtttcttt ttcttgaaaa tttttttttt tgattttttt ctctttcgat
     6481 gacctcccat tgatatttaa gttaataaac ggtcatcaat ttctcaagtt tcagtttcat
     6541 ttttcttgtt ctattacaac tttttttact tcttgctcat tagaaagaaa gcatagcaat
     6601 ctaatctaag ttttaattac aaaagatcta tgccaccacc atcaagaagt agaataaaca
     6661 aaacaagaac attaggaata gtgggtacag ctatagcagt gttggtcacg tcctactata
     6721 tatatcaaaa ggtgacaggt tctgtgtcca aaggagagga gttaatcaag gaaaacatga
     6781 gaatgaaagt tgtcatggag ggctccgtta atggtcacca attcaagtgt acaggggaag
     6841 gtgaaggtaa tccttacatg ggtacacaaa ctatgagaat taaagtaatt gaaggcggac
     6901 cactaccatt tgcatttgac attctggcaa cgtcattcat gtacggatca cgaactttca
     6961 tcaagtaccc taaaggtata ccagactttt tcaagcaatc ttttccagag ggttttacat
     7021 gggaaagggt tacaagatac gaagatgggg gtgtcgtcac agttatgcaa gatacttcat
     7081 tagaagatgg ctgccttgtc tatcatgtgc aagtaagagg ggtgaatttt ccttctaacg
     7141 gacctgtgat gcagaaaaag accaaaggtt gggaaccaaa tactgaaatg atgtacccag
     7201 ctgatggagg tttgagaggc tacacacaca tggcgcttaa agttgatggt ggaggtcatt
     7261 tgtcttgtag ttttgttacc acttatcgtt ctaaaaagac tgttggcaat atcaaaatgc
     7321 caggaataca tgctgtagac cacagactag aaagactcga agagagcgat aacgaaatgt
     7381 tcgttgtaca gagagagcat gccgtagcca aatttgctgg cttaggcggt ggtatggatg
     7441 aattgtataa gggatccggg tcaggctccg gatctggccc tcccccagct cttccgccaa
     7501 aaagacgaag gtaatggcag tgcttttaac taagaattat tagtcttttc tgcttatttt
     7561 ttcatcatag tttagaacac tttatattaa cgaatagttt atgaatctat ttaggtttaa
     7621 aaattgatac agttttataa gttacttttt caaagactcg tgctgtctat tgcataatgc
     7681 actggaaggg gaaaaaaaag gtgcacacgc gtggcttttt cttgaatttg cagtttgaaa
     7741 aatgctgagc agttacagag atgttacgaa ccactagtgc actgcagtac acggtttcct
     7801 tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga
     7861 cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc
     7921 ttaacccaac tgcacagaac aaaaacgtgc aggaaacgaa gataaatcat gtcgaaagct
     7981 acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc
     8041 atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta
     8101 ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc
     8161 ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac
     8221 aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag
     8281 tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg
     8341 gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct
     8401 agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat
     8461 actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct
     8521 caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg
     8581 ggtttagatg acaagggaga tgcattgggt caacagtata gaaccgtgga tgatgtggtt
     8641 tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct
     8701 aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc
     8761 cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga
     8821 gcttcaattt aattatatca gttattaccc gagtagagca cttgaatcca ctgccccggg
     8881 aatctcggtc gtaatgattt ctataatgac gaaaaaaaaa aaattggaaa gaaaaagctt
     8941 catggccttt ataaaaagga actatccaat acctcgccag aaccaagtaa cagtatttta
     9001 cggggcacaa atcaagaaca ataagacagg actgtaaaga tggacgcatt gaactccaaa
     9061 gaacaacaag agttccaaaa agtagtggaa caaaagcaaa tgaaggattt catgcgtttg
     9121 tactctaatc tggtagaaag atgtttcaca gactgtgtca atgacttcac aacatcaaag
     9181 ctaaccaata aggaacaaac atgcatcatg aagtgctcag aaaagttctt gaagcatagc
     9241 gaacgtgtag ggcagcgttt ccaagaacaa aacgctgcct tgggacaagg cttgggccga
     9301 taaggtgtac tggcgtatat atatctaatt atgtatctct ggtgtagccc atttttagca
     9361 tgtaaatata aagaccgagc ggccgcgtgt tacaaccaat taaccaattc tgattagaaa
     9421 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     9481 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     9541 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     9601 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     9661 ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta
     9721 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     9781 gcgaggcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     9841 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     9901 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     9961 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
    10021 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
    10081 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
    10141 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctggag
    10201 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
    10261 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
    10321 tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagtca
    10381 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10441 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10501 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10561 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10621 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10681 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10741 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10801 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10861 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10921 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10981 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    11041 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    11101 tgttctttcc tgcgttatcc cctgattctg tggataaccg tgcggccgcc aatgctaaat
    11161 tcgagtgaaa cacaggaaga tcagaaaatc ctcatttcat ccatattaac aataatttca
    11221 aatgtttatt tgcattattt gaaactaggc aagacaagca acgaaacgtt tttgaaaatt
    11281 ttgagtattt tcaataaatt tgtagaggac tcagatattg aaaaaaagct acagcaatta
    11341 atacttgata agaagagtat tgagaagggc aacggttcat catctcatgg atctgcacat
    11401 gaacaaacac cagagtcaaa cgacgttgaa attgaggcta ctgcgccaat tgatgacaat
    11461 acagacgatg ataacaaacc gaagttatct gatgtagaaa aggattaaag atgctaagag
    11521 atagtgatga tatttcataa ataatgtaat tctatatatg ttaattacct tttttgcgag
    11581 gcatatttat ggtgaaggat aagttttgac catcaaagaa ggttaatgtg gctgtggttt
    11641 cagggtccat aaagcccaca tggataacat tacccctgaa ttcgcatcta gactga
//
