LOCUS       sequence-491758-       12600 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12600)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 12600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-FilaminA-N-9
FEATURES             Location/Qualifiers
     source          1..12600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      8481..8490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8487..8489,8490..8492,8493..9206)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8532..8553)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8793..8812)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9140..9161
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    9328..9449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9365..9384)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9419..9438
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9456..9911)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9593..9614)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9805..9824
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9938..10042
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10044..10401
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10044..10235
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10067..10087)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10252..10387
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10436..11230
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10436..11230
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10490..10509)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11100..11119
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11418..11437)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11462..11509
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11838..12426
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12327..12346
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      541 ccatgagtag ctcccactct cgggcgggcc agagcgcagc aggcgcggct ccgggcggcg
      601 gcgtcgacac gcgggacgcc gagatgccgg ccaccgagaa ggacctggcg gaggacgcgc
      661 catggaagaa gatccagcag aacactttca cgcgctggtg caacgagcac ctgaagtgcg
      721 tgagcaagcg catcgccaac ctgcagacgg acctgagcga cgggctgcgg cttatcgcgc
      781 tgttggaggt gctcagccag aagaagatgc accgcaagca caaccagcgg cccactttcc
      841 gccaaatgca gcttgagaac gtgtcggtgg cactcgagtt cctggaccgc gagagcatca
      901 aactggtgtc catcgacagc aaggccatcg tggacgggaa cctgaagctg atcctgggcc
      961 tcatctggac cctgatcctg cactactcca tctccatgcc catgtgggac gaggaggagg
     1021 atgaggaggc caagaagcag acccccaagc agaggctcct gggctggatc cagaacaagc
     1081 tgccgcagct gcccatcacc aacttcagcc gggactggca gagcggccgg gccctgggcg
     1141 ccctggtgga cagctgtgcc ccgggcctgt gtcctgactg ggactcttgg gacgccagca
     1201 agcccgttac caatgcgcga gaggccatgc agcaggcgga tgactggctg ggcatccccc
     1261 aggtgatcac ccccgaggag attgtggacc ccaacgtgga cgagcactct gtcatgacct
     1321 acctgtccca gttccccaag gccaagctga agccaggggc tcccttgcgg cccaaactga
     1381 acccgaagaa agcccgtgcc tacgggccag gcatcgagcc cacaggcaac atggtgaaga
     1441 agcgggcaga gttcactgtg gagaccagaa gtgctggcca gggagaggtg ctggtgtacg
     1501 tggaggaccc ggccggacac caggaggagg caaaagtgac cgccaataac gacaagaacc
     1561 gcaccttctc cgtctggtac gtccccgagg tgacggggac tcataaggtt actgtgctct
     1621 ttgctggcca gcacatcgcc aagagcccct tcgaggtgta cgtggataag tcacagggtg
     1681 acgccagcaa agtgacagcc caaggtcccg gcctggagcc cagtggcaac atcgccaaca
     1741 agaccaccta ctttgagatc tttacggcag gagctggcac gggcgaggtc gaggttgtga
     1801 tccaggaccc catgggacag aagggcacgg tagagcctca gctggaggcc cggggcgaca
     1861 gcacataccg ctgcagctac cagcccacca tggagggcgt ccacaccgtg cacgtcacgt
     1921 ttgccggcgt gcccatccct cgcagcccct acactgtcac tgttggccaa gcctgtaacc
     1981 cgagtgcctg ccgggcggtt ggccggggcc tccagcccaa gggtgtgcgg gtgaaggaga
     2041 cagctgactt caaggtgtac acaaagggcg ctggcagtgg ggagctgaag gtcaccgtga
     2101 agggccccaa gggagaggag cgcgtgaagc agaaggacct gggggatggc gtgtatggct
     2161 tcgagtatta ccccatggtc cctggaacct atatcgtcac catcacgtgg ggtggtcaga
     2221 acatcgggcg cagtcccttc gaagtgaagg tgggcaccga gtgtggcaat cagaaggtac
     2281 gggcctgggg ccctgggctg gagggcggcg tcgttggcaa gtcagcagac tttgtggtgg
     2341 aggctatcgg ggacgacgtg ggcacgctgg gcttctcggt ggaagggcca tcgcaggcta
     2401 agatcgaatg tgacgacaag ggcgacggct cctgtgatgt gcgctactgg ccgcaggagg
     2461 ctggcgagta tgccgttcac gtgctgtgca acagcgaaga catccgcctc agccccttca
     2521 tggctgacat ccgtgacgcg ccccaggact tccacccaga cagggtgaag gcacgtgggc
     2581 ctggattgga gaagacaggt gtggccgtca acaagccagc agagttcaca gtggatgcca
     2641 agcacggtgg caaggcccca cttcgggtcc aagtccagga caatgaaggc tgccctgtgg
     2701 aggcgttggt caaggacaac ggcaatggca cttacagctg ctcctacgtg cccaggaagc
     2761 cggtgaagca cacagccatg gtgtcctggg gaggcgtcag catccccaac agccccttca
     2821 gggtgaatgt gggagctggc agccacccca acaaggtcaa agtatacggc cccggagtag
     2881 ccaagacagg gctcaaggcc cacgagccca cctacttcac tgtggactgc gccgaggctg
     2941 gccaggggga cgtcagcatc ggcatcaagt gtgcccctgg agtggtaggc cccgccgaag
     3001 ctgacatcga cttcgacatc atccgcaatg acaatgacac cttcacggtc aagtacacgc
     3061 cccggggggc tggcagctac accattatgg tcctctttgc tgaccaggcc acgcccacca
     3121 gccccatccg agtcaaggtg gagccctctc atgacgccag taaggtgaag gccgagggcc
     3181 ctggcctcag tcgcactggt gtcgagcttg gcaagcccac ccacttcaca gtaaatgcca
     3241 aagctgctgg caaaggcaag ctggacgtcc agttctcagg actcaccaag ggggatgcag
     3301 tgcgagatgt ggacatcatc gaccaccatg acaacaccta cacagtcaag tacacgcctg
     3361 tccagcaggg tccagtaggc gtcaatgtca cttatggagg ggatcccatc cctaagagcc
     3421 ctttctcagt ggcagtatct ccaagcctgg acctcagcaa gatcaaggtg tctggcctgg
     3481 gagagaaggt ggacgttggc aaagaccagg agttcacagt caaatcaaag ggtgctggtg
     3541 gtcaaggcaa agtggcatcc aagattgtgg gcccctcggg tgcagcggtg ccctgcaagg
     3601 tggagccagg cctgggggct gacaacagtg tggtgcgctt cctgccccgt gaggaagggc
     3661 cctatgaggt ggaggtgacc tatgacggcg tgcccgtgcc tggcagcccc tttcctctgg
     3721 aagctgtggc ccccaccaag cctagcaagg tgaaggcgtt tgggccgggg ctgcagggag
     3781 gcagtgcggg ctcccccgcc cgcttcacca tcgacaccaa gggcgccggc acaggtggcc
     3841 tgggcctgac ggtggagggc ccctgtgagg cgcagctcga gtgcttggac aatggggatg
     3901 gcacatgttc cgtgtcctac gtgcccaccg agcccgggga ctacaacatc aacatcctct
     3961 tcgctgacac ccacatccct ggctccccat tcaaggccca cgtggttccc tgctttgacg
     4021 catccaaagt caagtgctca ggccccgggc tggagcgggc caccgctggg gaggtgggcc
     4081 aattccaagt ggactgctcg agcgcgggca gcgcggagct gaccattgag atctgctcgg
     4141 aggcggggct tccggccgag gtgtacatcc aggaccacgg tgatggcacg cacaccatta
     4201 cctacattcc cctctgcccc ggggcctaca ccgtcaccat caagtacggc ggccagcccg
     4261 tgcccaactt ccccagcaag ctgcaggtgg aacctgcggt ggacacttcc ggtgtccagt
     4321 gctatgggcc tggtattgag ggccagggtg tcttccgtga ggccaccact gagttcagtg
     4381 tggacgcccg ggctctgaca cagaccggag ggccgcacgt caaggcccgt gtggccaacc
     4441 cctcaggcaa cctgacggag acctacgttc aggaccgtgg cgatggcatg tacaaagtgg
     4501 agtacacgcc ttacgaggag ggactgcact ccgtggacgt gacctatgac ggcagtcccg
     4561 tgcccagcag ccccttccag gtgcccgtga ccgagggctg cgacccctcc cgggtgcgtg
     4621 tccacgggcc aggcatccaa agtggcacca ccaacaagcc caacaagttc actgtggaga
     4681 ccaggggagc tggcacgggc ggcctgggcc tggctgtaga gggcccctcc gaggccaaga
     4741 tgtcctgcat ggataacaag gacggcagct gctcggtcga gtacatccct tatgaggctg
     4801 gcacctacag cctcaacgtc acctatggtg gccatcaagt gccaggcagt cctttcaagg
     4861 tccctgtgca tgatgtgaca gatgcgtcca aggtcaagtg ctctgggccc ggcctgagcc
     4921 caggcatggt tcgtgccaac ctccctcagt ccttccaggt ggacacaagc aaggctggtg
     4981 tggccccatt gcaggtcaaa gtgcaagggc ccaaaggcct ggtggagcca gtggacgtgg
     5041 tagacaacgc tgatggcacc cagaccgtca attatgtgcc cagccgagaa gggccctaca
     5101 gcatctcagt actgtatgga gatgaagagg taccccggag ccccttcaag gtcaaggtgc
     5161 tgcctactca tgatgccagc aaggtgaagg ccagtggccc cgggctcaac accactggcg
     5221 tgcctgccag cctgcccgtg gagttcacca tcgatgcaaa ggacgccggg gagggcctgc
     5281 tggctgtcca gatcacggat cccgaaggca agccgaagaa gacacacatc caagacaacc
     5341 atgacggcac gtatacagtg gcctacgtgc cagacgtgac aggtcgctac accatcctca
     5401 tcaagtacgg tggtgacgag atccccttct ccccgtaccg cgtgcgtgcc gtgcccaccg
     5461 gggacgccag caagtgcact gtcacaggtg ctggcatcgg ccccaccatt cagattgggg
     5521 aggagacggt gatcactgtg gacactaagg cggcaggcaa aggcaaagtg acgtgcaccg
     5581 tgtgcacgcc tgatggctca gaggtggatg tggacgtggt ggagaatgag gacggcactt
     5641 tcgacatctt ctacacggcc ccccagccgg gcaaatacgt catctgtgtg cgctttggtg
     5701 gcgagcacgt gcccaacagc cccttccaag tgacggctct ggctggggac cagccctcgg
     5761 tgcagccccc tctacggtct cagcagctgg ccccacagta cacctacgcc cagggcggcc
     5821 agcagacttg ggccccggag aggcccctgg tgggtgtcaa tgggctggat gtgaccagcc
     5881 tgaggccctt tgaccttgtc atccccttca ccatcaagaa gggcgagatc acaggggagg
     5941 ttcggatgcc ctcaggcaag gtggcgcagc ccaccatcac tgacaacaaa gacggcaccg
     6001 tgaccgtgcg gtatgcaccc agcgaggctg gcctgcacga gatggacatc cgctatgaca
     6061 acatgcacat cccaggaagc cccttgcagt tctatgtgga ttacgtcaac tgtggccatg
     6121 tcactgccta tgggcctggc ctcacccatg gagtagtgaa caagcctgcc accttcaccg
     6181 tcaacaccaa ggatgcagga gaggggggcc tgtctctggc cattgagggc ccgtccaaag
     6241 cagaaatcag ctgcactgac aaccaggatg ggacatgcag cgtgtcctac ctgcctgtgc
     6301 tgccggggga ctacagcatt ctagtcaagt acaatgaaca gcacgtccca ggcagcccct
     6361 tcactgcccg ggtcacaggt gacgactcca tgcgtatgtc ccacctaaag gtcggctctg
     6421 ctgccgacat ccccatcaac atctcagaga cggatctcag cctgctgacg gccactgtgg
     6481 tcccgccctc gggccgggag gagccctgtt tgctgaagcg gctgcgtaat ggccacgtgg
     6541 ggatttcatt cgtgcccaag gagacggggg agcacctggt gcatgtgaag aaaaatggcc
     6601 agcacgtggc cagcagcccc atcccggtgg tgatcagcca gtcggaaatt ggggatgcca
     6661 gtcgtgttcg ggtctctggt cagggccttc acgaaggcca cacctttgag cctgcagagt
     6721 ttatcattga tacccgcgat gcaggctatg gtgggctcag cctgtccatt gagggcccca
     6781 gcaaggtgga catcaacaca gaggacctgg aggacgggac gtgcagggtc acctactgcc
     6841 ccacagagcc aggcaactac atcatcaaca tcaagtttgc cgaccagcac gtgcctggca
     6901 gccccttctc tgtgaaggtg acaggcgagg gccgggtgaa agagagcatc acccgcaggc
     6961 gtcgggctcc ttcagtggcc aacgttggta gtcattgtga cctcagcctg aaaatccctg
     7021 aaattagcat ccaggatatg acagcccagg tgaccagccc atcgggcaag acccatgagg
     7081 ccgagatcgt ggaaggggag aaccacacct actgcatccg ctttgttccc gctgagatgg
     7141 gcacacacac agtcagcgtc aagtacaagg gccagcacgt gcctgggagc cccttccagt
     7201 tcaccgtggg gcccctaggg gaagggggag cccacaaggt ccgagctggg ggccctggcc
     7261 tggagagagc tgaagctgga gtgccagccg aattcagtat ctggacccgg gaagctggtg
     7321 ctggaggcct ggccattgct gtcgagggcc ccagcaaggc tgagatctct tttgaggacc
     7381 gcaaggacgg ctcctgtggt gtggcttatg tggtccagga gccaggtgac tacgaagtct
     7441 cagtcaagtt caacgaggaa cacattcccg acagcccctt cgtggtgcct gtggcttctc
     7501 cgtctggcga cgcccgccgc ctcactgttt ctagccttca ggagtcaggg ctaaaggtca
     7561 accagccagc ctcttttgca gtcagcctga acggggccaa gggggcgatc gatgccaagg
     7621 tgcacagccc ctcaggagcc ctggaggagt gctatgtcac agaaattgac caagataagt
     7681 atgctgtgcg cttcatccct cgggagaatg gcgtttacct gattgacgtc aagttcaacg
     7741 gtacccacat ccctggaagc cccttcaaga tccgagttgg ggagcctggg catggagggg
     7801 acccaggctt ggtgtctgct tacggagcag gtctggaagg cggtgtcaca gggaacccag
     7861 ctgagttcgt cgtgaacacg agcaatgcgg gagctggtgc cctgtcggtg accattgacg
     7921 gcccctccaa ggtgaagatg gattgccagg agtgccctga gggctaccgc gtcacctata
     7981 cccccatggc acctggcagc tacctcatct ccatcaagta cggcggcccc taccacattg
     8041 ggggcagccc cttcaaggcc aaagtcacag gcccccgtct cgtcagcaac cacagcctcc
     8101 acgagacatc atcagtgttt gtagactctc tgaccaaggc cacctgtgcc ccccagcatg
     8161 gggccccggg tcctgggcct gctgacgcca gcaaggtggt ggccaagggc ctggggctga
     8221 gcaaggccta cgtaggccag aagagcagct tcacagtaga ctgcagcaaa gcaggcaaca
     8281 acatgctgct ggtgggggtt catggcccaa ggaccccctg cgaggagatc ctggtgaagc
     8341 acgtgggcag ccggctctac agcgtgtcct acctgctcaa ggacaagggg gagtacacac
     8401 tggtggtcaa atgggggcac gagcacatcc caggcagccc ctaccgcgtt gtggtgccca
     8461 gcggcagcgc ctcaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     8521 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     8581 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     8641 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct
     8701 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     8761 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     8821 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     8881 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct
     8941 atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca
     9001 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     9061 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
     9121 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     9181 tcggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc
     9241 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     9301 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     9361 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9421 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt
     9481 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     9541 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     9601 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     9661 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     9721 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     9781 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     9841 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     9901 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     9961 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
    10021 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
    10081 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    10141 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10201 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    10261 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    10321 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    10381 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
    10441 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
    10501 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
    10561 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
    10621 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
    10681 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
    10741 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
    10801 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
    10861 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
    10921 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
    10981 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
    11041 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
    11101 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
    11161 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
    11221 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
    11281 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
    11341 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    11401 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
    11461 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
    11521 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
    11581 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
    11641 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
    11701 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    11761 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    11821 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    11881 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    11941 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    12001 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    12061 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    12121 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    12181 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    12241 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    12301 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    12361 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    12421 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    12481 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
    12541 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
//
