LOCUS       sequence-491763-        5945 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  5' AAVLINK plasmid for SETD5 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Zhonghua Lu Lab
  TITLE     AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery 
            in Gene Therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVLINK-EF1alpha-HA-5'-SETD5
FEATURES             Location/Qualifiers
     source          1..5945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      3423..3878
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3510..3529)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3720..3741
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3895..3914)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4014..4036
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4074..4092)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4160..4264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4265..4333,4334..5125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4483..4502)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5296..5884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5785..5804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctagca ttgcaatccc tctgggagtc accacatcag atacatccta
      781 ctcagatatg gctgctggat cagaccctga atctgtggag gctagtccag cagttaatga
      841 gaagagcgtg tattccactc ataattatgg gaccactcag aggcatgggt gtcgaggact
      901 gccttatgct acgatcatcc ctcgttctga cctgaatggc ctgccgtcgc ctgtagagga
      961 acgctgtgga gacagcccga actctgaagg agaaactgta cctacctggt gtccttgtgg
     1021 tctttctcag gatggcttcc ttctcaactg tgacaagtgc aggggaatga gcagggggaa
     1081 ggttattaga cttcatcggc ggaagcagga caacatatca ggtggggata gcagtgcaac
     1141 agaaagctgg gatgaggagc tttctccttc cactgtgttg tatacagcaa cacagcacac
     1201 acctacaagc atcaccttaa ctgttagaag aaccaaaccc aagaagcgga aaaagagtcc
     1261 agaaaagggt cgtgcagcac caaagacgaa gaaaatcaag aattctccct ctgaagcaca
     1321 gaatttagat gagaatacaa ctgagggctg ggaaaatcgg ataagactat ggactgacca
     1381 gtatgaagaa gctttcacta atcagtacag tgcagatgta cagaacgcgc ttgaacaaca
     1441 cctacattct agcaaggaat ttgtgggcaa acctactatt ttagacacta ttaataagac
     1501 tgaattggcc tgtaataaca cagttattgg ttcccaaatg cagttacagc tgggaagagt
     1561 cactcgtgtt caaaagcacc ggaagatcct gagggctgca agagatttgg ctttggacac
     1621 tcttataata gagtatcgtg ggaaagtcat gttacgacag caatttgagg tcaatgggca
     1681 tttcttcaaa aaaccatacc cctttgtgct cttctactca aaattcaatg gtgtagagat
     1741 gtgtgtggat gcccgtactt tcggtaatga tgctcggttc atcagaagat catgtacacc
     1801 aaatgcagag gtgcgacaca tgattgcaga tgggatgatt cacctgtgca tctatgctgt
     1861 gtctgccatc accaaggatg ctgaggtcac catagcattt gattatgagt atagtaactg
     1921 taattataaa gtggactgtg cctgtcacaa gggaaaccgg aattgtccta tacaaaaaag
     1981 gaatcctaat gctacagaac tgccactcct accacctcct ccaagcctac ccaccattgg
     2041 agcagagact agacgtagaa aagcacgacg gaaagagcta gagatggagc agcagaatga
     2101 ggcttcagag gagaataatg accagcaatc acaagaagtt ccagaaaaag taactgtatc
     2161 cagtgatcat gaggaagtag acaatccaga agaaaaacca gaagaagaga aagaagaggt
     2221 tatagatgac caggagaacc tagctcatag caggaggacc agggaagata gaaaggtaga
     2281 agccatcatg catgcttttg aaaacttaga gaaaagaaag aagcggcggg atcagccctt
     2341 ggaacagagc aactctgatg tagagattac tacaaccacc tcagagactc ctgttggtga
     2401 agagacaaaa actgaagccc ctgaatctga agttagcaac tctgtttcaa atgttaccat
     2461 cccaagcacc ccacagagtg ttggtgtgaa tacccggagg tcttcccaag caggggatat
     2521 tgctgcagaa aaactagtcc ccaagccacc tccagcaaag ccttctaggc cccggccgaa
     2581 gagtcgaatt tctcggtaca ggaccagttc agcccaaaga ctaaagcgtc agaagcaggc
     2641 caatgcacag caggcagaat tgtcacaagc tgccttggaa gagggaggaa gtaacagttt
     2701 agtaactcct actgaagctg gaagtctaga cagttcagga gaaaacaggc cattaacagg
     2761 gtctgaccca actgtggtgt caattactgg atcccatgtc aaccgtgctg catctaaata
     2821 ccccaaaacc aaaaagtatc tagttacaga atggttgaat gacaaagcag agaagcaaga
     2881 gtgccctgtt gagtgccctt tacgtatcac aacggatcca actgtactgg caacgaccct
     2941 aaacatgtta ccaggtctta tccattcccc gttaatttgc accaccccca aacactacat
     3001 tcgctttggc tcacccttta tccctgagag acgtcgaagg ccccttctgc ctgatggcac
     3061 attcagctcc tgtaagaagc gctggataaa acaagcctta gaagaaggta agtatcaagg
     3121 ttacaagaca ggtttaagga gaccaataag ctattattcg tataggatac tttatacgaa
     3181 gttattaagt tatggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta
     3241 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     3301 aaggtcgccc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     3361 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     3421 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3481 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3541 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3601 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     3661 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3721 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
     3781 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
     3841 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     3901 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     3961 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     4021 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     4081 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     4141 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4201 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4261 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4321 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4381 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4441 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4501 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4561 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4621 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4681 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4741 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4801 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4861 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4921 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4981 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5041 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5101 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5161 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5221 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5281 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5341 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5401 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5461 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5521 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5581 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5641 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5701 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5761 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5821 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5881 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5941 catgt
//
