LOCUS       sequence-491920-        8701 bp    DNA     circular SYN 05-NOV-2025
DEFINITION  Expression vector for mouse Pgc-1alpha with EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Chandra R, Engeln M, Francis TC, Konkalmatt P, Patel D, Lobo MK
  TITLE     A Role for Peroxisome Proliferator-Activated Receptor Gamma 
            Coactivator-1alpha in Nucleus Accumbens Neuron Subtypes in Cocaine 
            Action.
  JOURNAL   Biol Psychiatry. 2017 Apr 1;81(7):564-572. doi: 
            10.1016/j.biopsych.2016.10.024. Epub 2016 Oct 28.
  PUBMED    27939396
REFERENCE   2  (bases 1 to 8701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.biopsych.2016.10.024"; journalName: "Biol Psychiatry"; 
            date: "2017-04-1- 1"; volume: "81"; issue: "7"; pages: "564-572"
COMMENT     Sequence Label: pAAV-Ef1a-DIO-Pgc-1alpha-EYFP
FEATURES             Location/Qualifiers
     source          1..8701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   674..803
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(865..1453)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(945..964)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1624..2415,2416..2484))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2247..2266
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2485..2589)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2657..2675
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2713..2735)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2835..2854
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2871..3326)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3008..3029)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3220..3239
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   3401..3541
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3412..3541
                     /label=AAV2 ITR
     polyA_signal    3581..4057
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     3839..3858
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     misc_feature    4089..4677
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4195..4206
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4604..4624
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    complement(4702..4735)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(4786..4819)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             join(7220..7222,7223..7225,7226..7939)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7265..7286)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7526..7545)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7873..7894
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    7948..7981
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    8032..8065
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     promoter        complement(join(8096..8701,1..573))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(8105..8701,1..342)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(8128..8148)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cagatcttgg cccgcattta caagactatc ttgccagaaa aaaagcgtcg cagcaggtca
       61 tcaaaaattt taaatggcta gagacttatc gaaagcagcg agacaggcgc gaaggtgcca
      121 ccagattcgc acgcggcggc cccagcgccc aggccaggcc tcaactcaag cacgaggcga
      181 aggggctcct taagcgcaag gcctcgaact ctcccaccca cttccaaccc gaagctcggg
      241 atcaagaatc acgtactgca gccaggtgga agtaattcaa ggcacgcaag ggccataacc
      301 cgtaaagagg ccaggcccgc gggaaccaca cacggcactt acctgtgttc tggcggcaaa
      361 cccgttgcga aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc
      421 ctcgggaaaa aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct
      481 tctctaggca ccggttcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga
      541 tgtgcgctct gcccactgac gggcaccgga gccaattccc actcctttca agacctagaa
      601 ggtccattag ctgcaaagat tcctctctgt ttaaaacttt atccatcttt gcaaagctta
      661 cgcgtgcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
      721 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggcggcct cagtgagcga
      781 gcgagcgcgc agctgcctgc aggacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      841 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      901 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      961 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1021 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1081 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1141 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1201 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1261 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1321 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1381 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1441 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1501 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1561 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1621 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1681 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     1741 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     1801 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     1861 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     1921 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     1981 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2041 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2101 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2161 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2221 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2281 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2341 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2401 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2461 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2521 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2581 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     2641 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     2701 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     2761 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     2821 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata aaattgtaaa
     2881 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     2941 atagaccgaa atcggcaaaa tcccttataa atcaaaagaa tagcccgaga tagagttgag
     3001 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3061 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt
     3121 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     3181 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     3241 gggcgctaag gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     3301 gcttaatgcg ccgctacagg gcgcgtacta tggttgcttt gacgtatgcg gtgtgaaata
     3361 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc cctgcaggca gctgcgcgct
     3421 cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg
     3481 gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc
     3541 tgcggccgct cggtccgcac gtggttacct acaaaatcag aaggacaggg aagggagcag
     3601 tggttcacgc ctgtaatccc agcaatttgg gaggccaagg tgggtagatc acctgagatt
     3661 aggagttgga gaccagcctg gccaatatgg tgaaaccccg tctctaccaa aaaaacaaaa
     3721 attagctgag cctggtcatg catgcctgga atcccaacaa ctcgggaggc tgaggcagga
     3781 gaatcgcttg aacccaggag gcggagattg cagtgagcca agattgtgcc actgcactcc
     3841 agcttggttc ccaatagacc ccgcaggccc tacaggttgt cttcccaact tgccccttgc
     3901 tccataccac ccccctccac cccataatat tatagaagga cacctagtca gacaaaatga
     3961 tgcaacttaa ttttattagg acaaggctgg tgggcactgg agtggcaact tccagggcca
     4021 ggagaggcac tggggagggg tcacagggat gccacccgta gatctctcga gcagcgctcg
     4081 gtatcgatgc ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag
     4141 acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag
     4201 ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cataggcgag
     4261 cagccaagga aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag
     4321 ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg
     4381 gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt
     4441 gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa
     4501 ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga aagccatacg
     4561 ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc
     4621 aacatagtta agaataccag tcaatctttc acaaattttg taatccagag gttgattatc
     4681 gataagcttg atatcgaatt cataacttcg tatagcatac attatacgaa gttattcttt
     4741 gcaccattct aaagaataac agtgataatt tctgggttaa ggcaaataac ttcgtatagg
     4801 atactttata cgaagttatg ctagtatggc ttgggacatg tgcagccaag actctgtatg
     4861 gagtgacata gagtgtgctg ctctggttgg tgaggaccag cctctttgcc cagatcttcc
     4921 tgaacttgac ctttctgaac ttgatgtgaa tgacttggat acagacagct ttctgggtgg
     4981 attgaagtgg tgtagcgacc aatcggaaat catatccaac cagtacaaca atgagcctgc
     5041 gaacatattt gagaagatag atgaagagaa tgaggcaaac ttgctagcgg tcctcacaga
     5101 gacactggac agtctccccg tggatgaaga cggattgccc tcatttgatg cactgacaga
     5161 tggagccgtg accactgaca acgaggccag tccttcctcc atgcctgacg gcacccctcc
     5221 ccctcaggag gcagaagagc cgtctctact taagaagctc ttactggcac cagccaacac
     5281 tcagctcagc tacaatgaat gcagcggtct tagcactcag aaccatgcag caaaccacac
     5341 ccacaggatc agaacaaacc ctgccattgt taagaccgag aattcatgga gcaataaagc
     5401 gaagagcatt tgtcaacagc aaaagccaca aagacgtccc tgctcagagc ttctcaagta
     5461 tctgaccaca aacgatgacc ctcctcacac caaacccaca gaaaacagga acagcagcag
     5521 agacaaatgt gcttccaaaa agaagtccca tacacaaccg cagtcgcaac atgctcaagc
     5581 caaaccaaca actttatctc ttcctctgac cccagagtca ccaaatgacc ccaagggttc
     5641 cccatttgaa aacaagacta ttgagcgaac cttaagtgtg gaactctctg gaactgcagg
     5701 cctaactcct cccacaactc ctcctcataa agccaaccaa gataaccctt tcaaggcttc
     5761 gccaaagctg aagccctctt gcaagaccgt ggtgccaccg ccaaccaaga gggcccggta
     5821 cagtgagtgt tctggtacca aaggcagcca ctccaccaag aaagggcccg agcaatctga
     5881 gttgtacgca caactcagca agtcctcagg gctcagccga ggacacgagg aaaggaagac
     5941 taaacggccc agtctccggc tgtttggtga ccatgactac tgtcagtcac tcaattccaa
     6001 aacggatata ctcattaaca tatcacagga gctccaagac tctagacaac tagacttcaa
     6061 agatgcctcc tgtgactggc aggggcacat ctgttcttcc acagattcag gccagtgcta
     6121 cctgagagag actttggagg ccagcaagca ggtctctcct tgcagcacca gaaaacagct
     6181 ccaagaccag gaaatccgag cggagctgaa caagcacttc ggtcatccct gtcaagctgt
     6241 gtttgacgac aaatcagaca agaccagtga actaagggat ggcgacttca gtaatgaaca
     6301 attctccaaa ctacctgtgt ttataaattc aggactagcc atggatggcc tatttgatga
     6361 cagtgaagat gaaagtgata aactgagcta cccttgggat ggcacgcagc cctattcatt
     6421 gttcgatgtg tcgccttctt gctcttcctt taactctccg tgtcgagact cagtgtcacc
     6481 accgaaatcc ttattttctc aaagacccca aaggatgcgc tctcgttcaa gatccttttc
     6541 tcgacacagg tcgtgttccc gatcaccata ttccaggtca agatcaaggt ccccaggcag
     6601 tagatcctct tcaagatcct gttactacta tgaatcaagc cactacagac accgcacaca
     6661 ccgcaattct cccttgtatg tgagatcacg ttcaaggtca ccctacagcc gtaggcccag
     6721 gtacgacagc tatgaagcct atgagcacga aaggctcaag agggatgaat accgcaaaga
     6781 gcacgagaag cgggagtctg aaagggccaa acagagagag aggcagaagc agaaagcaat
     6841 tgaagagcgc cgtgtgattt acgttggtaa aatcagacct gacacaacgc ggacagaatt
     6901 gagagaccgc tttgaagttt ttggtgaaat tgaggaatgc accgtaaatc tgcgggatga
     6961 tggagacagc tatggtttca tcacctaccg ttacacctgt gacgctttcg ctgctcttga
     7021 gaatggatat actttacgca ggtcgaacga aactgacttc gagctgtact tttgtggacg
     7081 gaagcaattt ttcaagtcta actatgcaga cctagatacc aactcagacg attttgaccc
     7141 tgcttccacc aagagcaagt atgactctct ggattttgat agtttactga aggaagctca
     7201 gagaagcttg cgcaggttca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     7261 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     7321 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     7381 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg
     7441 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     7501 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     7561 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     7621 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     7681 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     7741 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     7801 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     7861 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     7921 ggacgagctg tacaagtaag gcgcgccata acttcgtata atgtatgcta tacgaagtta
     7981 ttaagaggtt tcatattgct aatagcagct acaatccagc taccattctg cataacttcg
     8041 tataaagtat cctatacgaa gttattccgg agtcgactct agaggatccg gtacctcacg
     8101 acacctgaaa tggaagaaaa aaactttgaa ccactgtctg aggcttgaga atgaaccaag
     8161 atccaaactc aaaaagggca aattccaagg agaattacat caagtgccaa gctggcctaa
     8221 cttcagtctc cacccactca gtgtggggaa actccatcgc ataaaacccc tccccccaac
     8281 ctaaagacga cgtactccaa aagctcgaga actaatcgag gtgcctggac ggcgcccggt
     8341 actccgtgga gtcacatgaa gcgacggctg aggacggaaa ggcccttttc ctttgtgtgg
     8401 gtgactcacc cgcccgctct cccgagcgcc gcgtcctcca ttttgagctc cctgcagcag
     8461 ggccgggaag cggccatctt tccgctcacg caactggtgc cgaccgggcc agccttgccg
     8521 cccagggcgg ggcgatacac ggcggcgcga ggccaggcac cagagcaggc cggccagctt
     8581 gagactaccc ccgtccgatt ctcggtggcc gcgctcgcag gccccgcctc gccgaacatg
     8641 tgcgctggga cgcacgggcc ccgtcgccgc ccgcggcccc aaaaaccgaa ataccagtgt
     8701 g
//
